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All Journal HAYATI Journal of Biosciences Jurnal Penelitian Sains (JPS) Microbiology Indonesia SEMIRATA 2015 Jurnal Penelitian Sains (JPS) IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) Jurnal Penelitian Sains Sainmatika: Jurnal Ilmiah Matematika dan Ilmu Pengetahuan Alam Molekul: Jurnal Ilmiah Kimia JPPM (Jurnal Pengabdian dan Pemberdayaan Masyarakat) Jurnal Pengabdian Masyarakat: Humanity and Medicine SRIWIJAYA JOURNAL OF ENVIRONMENT BIOVALENTIA: Biological Research Journal Joong-Ki : Jurnal Pengabdian Masyarakat Jurnal Riset Rumpun Ilmu Kesehatan (JURRIKES) Jurnal Pepadu Journal of Local Therapy Joong-Ki
Heni Yohandini
Department Of Chemistry, Faculty Of Mathematics And Natural Sciences, University Of Sriwijaya, Indralaya, Ogan Ilir, South Sumatra, 30662 Indonesia
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences

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ISOLASI DAN IDENTIFIKASI BAKTERI TERMO-LIPOLITIK DENGAN PENDEKATAN BIOLOGI MOLEKULER BERBASIS GEN 16S rRNA Meita Anggraini, Muharni, Heni Yohandini,
SEMIRATA 2015 Prosiding Bidang Biologi
Publisher : SEMIRATA 2015

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (209.151 KB)

Abstract

Bakteri termo-lipolitik merupakan bakteri pendegradasi lipid yang mampu beraktivitas pada suhu tinggi. Bakteri tersebut memiliki peran penting dalam perkembangan bioteknologi pada saat ini, karena dapat diaplikasikan pada berbagai industri baik industri pangan maupun non pangan.  Salah satu upaya yang dilakukan pada penelitian ini adalah mencari dan menyeleksi isolat-isolat bakteri termofilik yang memiliki aktivitas lipolitik, serta mengetahui jenis dari masing-masing isolat tersebut. Seleksi dilakukan pada medium ZoBell padat yang mengandung olive oil 1% dengan menggunakan metode pour plate dan identifikasi isolat melalui analisis sekuen gen 16S-rRNA menggunakan DNA sequencer.  Hasil isolasi memperoleh 6 isolat bakteri termofilik, dan hanya 4 isolat yang memiliki aktivitas lipolitik dengan indeks lipolitik tertinggi 2,65.  Analisis similaritas berdasarkan sekuen gen 16S-rRNA menunjukkan bahwa dari isolat bakteri termo-lipolitik pada penelitian ini memiliki kemiripan dengan Anoxybacillus pushchinoensis 99% (untuk isolat AA1 dan AA3), Anoxybacillus sp. 99% (untuk isolat AB2) dan Bacillus licheniformis 99% (untuk isolat LB1). Kata kunci : Identifikasi, bakteri termo-lipolitik, gen 16S rRNA
β- Sitosterol and Betulonic Acid from n-Hexane Extract the Stem Bark of Tetracera indica Muharni, Muharni; elfita, elfita; Julunar, Julinar; Yohandini, Heni; oktaviani, muthia
Molekul Vol 14, No 2 (2019)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (615.289 KB) | DOI: 10.20884/1.jm.2019.14.2.507

Abstract

The Tetraceraindicahasbeen used traditionally to treat various diseases including skin medications, lower blood pressure, fever, headache, wound andgout medicine.   The objective of the research was isolatedand characterizedthe ?-sitosterol (1) and betulonicacid (2) fromn-hexaneextract of the stem bark of Tetraceraindica.  The dry powder thestembark (550 g)wasmaceration with n-hexane for 24 hrs.Thisextraction process was repeated three times.  The n-hexane extract was separated and purified by chromatographytechnique. The structure of isolatedcompound wasdetermined by spectroscopy data UV, IR, NMR and compared with reported data.  Two compounds have been isolated and base on spectroscopy data thecompounds is ?-sitosterol (1) andbetulonic acid (2).   Betulonic acid is being reported for the first time from T.indica,meanwhile the?-sitosterolpreviously reported from MeOHextractof leaves of T. indicabut  is being reported for the first time from the stembarkof  T.indica.
CONDITION OPTIMIZATION PRODUCTION XYLANASE THERMOSTABLE BY Bacillus licheniformis TS10 USING SUBSTRATE OIL PALM EMPTY FRUIT BUNCHES Safitri, Ayu; Muharni, -; Kusumawati, Heni Yohandini
BIOVALENTIA: Biological Research Journal Vol. 3 No. 1 (2017)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (133.065 KB) | DOI: 10.24233/BIOV.3.1.2017.51

Abstract

Optimization conditions for thermostable xylanase production of Bacillus licheniformis TS10 by using substrate oil palm empty fruit bunches  has been conducted from November 2015 to January 2016 in the Laboratory of Genetics and Biotechnology, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Sriwijaya, Indralaya. The aims of this study was to determine the potential of oil palm empty fruit bunches ( EFB ) as a substrate for the production of thermostable xylanase by Bacillus licheniformis TS10 and the optimum conditions of temperature, pH and substrate concentration in the fermentation process. The research method is to make the growth curve and the curve of Bacillus licheniformis TS10 enzyme production on EFB substrate. The number of bacterial cells was determined by using a UV-VIS spectrophotometer and total plate count (TPC) through a standard curve. Optimization performed at various pH (5, 6, 7, 8, 9), temperature (50 °C, 60 °C, 70 °C, 80 °C) and substrate (1%, 2%, 3%, 4%) , Test activity at each pH, temperature and substrate using methods DNS by measuring enzyme activity based on the reducing sugar released by the substrate by using dinitrosalicylic acid (DNS). Based on the results of this research showed that oil palm empty fruit bunches (EFB ) has potential as a substrate for the production of thermostable xylanase by Bacillus licheniformis TS10, thermostable xylanase production of Bacillus licheniformis TS10 on the substrate EFB has an optimum condition at pH 6, 80 °C and the substrate concentration of 4%.
CONDITION OPTIMIZATION PRODUCTION XYLANASE THERMOSTABLE BY Bacillus licheniformis TS10 USING SUBSTRATE OIL PALM EMPTY FRUIT BUNCHES Safitri, Ayu; Muharni, -; Kusumawati, Heni Yohandini
BIOVALENTIA: Biological Research Journal Vol. 3 No. 1 (2017)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (133.065 KB) | DOI: 10.24233/BIOV.3.1.2017.51

Abstract

Optimization conditions for thermostable xylanase production of Bacillus licheniformis TS10 by using substrate oil palm empty fruit bunches  has been conducted from November 2015 to January 2016 in the Laboratory of Genetics and Biotechnology, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Sriwijaya, Indralaya. The aims of this study was to determine the potential of oil palm empty fruit bunches ( EFB ) as a substrate for the production of thermostable xylanase by Bacillus licheniformis TS10 and the optimum conditions of temperature, pH and substrate concentration in the fermentation process. The research method is to make the growth curve and the curve of Bacillus licheniformis TS10 enzyme production on EFB substrate. The number of bacterial cells was determined by using a UV-VIS spectrophotometer and total plate count (TPC) through a standard curve. Optimization performed at various pH (5, 6, 7, 8, 9), temperature (50 °C, 60 °C, 70 °C, 80 °C) and substrate (1%, 2%, 3%, 4%) , Test activity at each pH, temperature and substrate using methods DNS by measuring enzyme activity based on the reducing sugar released by the substrate by using dinitrosalicylic acid (DNS). Based on the results of this research showed that oil palm empty fruit bunches (EFB ) has potential as a substrate for the production of thermostable xylanase by Bacillus licheniformis TS10, thermostable xylanase production of Bacillus licheniformis TS10 on the substrate EFB has an optimum condition at pH 6, 80 °C and the substrate concentration of 4%.
BIODECOLORIZATION OF TEXTILE INDUSTRIAL WASTE BY THERMOPHILIC BACTERIA Anoxybacillus rupiensis TS04 AND Anoxybacillus flavithermus TS15 Muharni, Muharni; Yohandini, Heni; Rivai, M Yunus
BIOVALENTIA: Biological Research Journal Vol. 4 No. 1 (2018)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (351.91 KB) | DOI: 10.24233/BIOV.4.1.2018.96

Abstract

Wastewater of textile industries contained a high content of synthetic dyes that could damage the aquatic ecosystem and environmental aesthetics. The use of microorganisms on the biodecolorization of textile industrial waste had advantages such as low cost and environmentally friendly. The purpose of the study was to determine biodecolorization capability of Anoxybacillus rupiensis TS04 and Anoxybacillus flavithermus TS15 for industrial waste of tie-dye fabrics. Completely randomized design with factorial pattern was used in the research; factor I and II were concentrations of wastewater and types of bacteria, respectively Anoxybacillus rupiensis TS04 showed the highest decolorization ability by 83.25% for wastewater concentration of 80% (v/v) and Anoxybacillus flavithermus TS15 by 69% at 40% (v/v) waste concentration.The highest cell number of Anoxybacillus rupiensis TS04 was obtained as 1.52 x 106 cfu/mL and biodecolorization, textile industrial waste, thermophilic bacteria,Anoxybacillus flavithermus TS15 3.70 x 105cfu/mL.
Isolation and Phylogenetic Analysis of Thermophile Community Within Tanjung Sakti Hot Spring, South Sumatera, Indonesia Heni Yohandini; . Julinar; . Muharni
HAYATI Journal of Biosciences Vol. 22 No. 3 (2015): July 2015
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2020.716 KB) | DOI: 10.4308/hjb.22.3.143

Abstract

A community of thermophiles within Tanjung Sakti Hot Spring (South Sumatera) have been cultivated and identified based on 16S ribosomal RNA gene sequence. The hot spring has temperature 80C-91C and pH 7-8. We used a simple method for culturing the microbes, by enriching the spring water with nutrient broth media. Phylogenetic analysis showed that the method could recover microbes, which clustered within four distinct taxonomic groups: Anoxybacillus, Geobacillus, Brevibacillus, and Bacillus. These microbes closely related to Anoxybacillus rupiensis, Anoxybacillus flavithermus, Geobacillus pallidus, Brevibacillus thermoruber, Bacillus licheniformis, and Bacillus thermoamylovorans. The 16S ribosomal RNA gene sequence of one isolate only had 96% similarity with Brevibacillus sequence in GenBank.
Isolation and Phylogenetic Analysis of Thermophile Community Within Tanjung Sakti Hot Spring, South Sumatera, Indonesia Heni Yohandini; . Julinar; . Muharni
HAYATI Journal of Biosciences Vol. 22 No. 3 (2015): July 2015
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2020.716 KB) | DOI: 10.4308/hjb.22.3.143

Abstract

A community of thermophiles within Tanjung Sakti Hot Spring (South Sumatera) have been cultivated and identified based on 16S ribosomal RNA gene sequence. The hot spring has temperature 80C-91C and pH 7-8. We used a simple method for culturing the microbes, by enriching the spring water with nutrient broth media. Phylogenetic analysis showed that the method could recover microbes, which clustered within four distinct taxonomic groups: Anoxybacillus, Geobacillus, Brevibacillus, and Bacillus. These microbes closely related to Anoxybacillus rupiensis, Anoxybacillus flavithermus, Geobacillus pallidus, Brevibacillus thermoruber, Bacillus licheniformis, and Bacillus thermoamylovorans. The 16S ribosomal RNA gene sequence of one isolate only had 96% similarity with Brevibacillus sequence in GenBank.
Analisis Kualitatif dan Kuantitatif Asam Lemak Tak Jenuh Omega-3, Omega-6 dan Karakterisasi Minyak Ikan Patin (Pangasius pangasius) Almunady T. Panagan; Heni Yohandini; Mila Wulandari
Jurnal Penelitian Sains Vol 15, No 3 (2012)
Publisher : Faculty of Mathtmatics and Natural Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (146.582 KB) | DOI: 10.56064/jps.v15i3.105

Abstract

Telah dilakukan analisis kualitatif dan kuantitatif asam lemak tak jenuh Omega-3 dan Omega-6 pada minyak ikan patin (Pangasius pangasius) dengan menggunakan metode kromatografi gas. Kadar Omega-3 yang diperoleh dari minyak ikan patin yaitu berkisar antara 1,16-12,44(%W/W) dan kadar Omega-6 berkisaran antara 12,278-15,961(%W/W). Dari kadar Omega-3 dan dari kadar Omega-6 yang diperoleh dari penelitian ini, ikan patin dapat digunakan sebagai sumber Omega-3 dan Omega-6 alternatif. Dalam penelitian ini juga dilakukan karakterisasi minyak ikan patin yang meliputi kadar minyak, bilangan asam, bilangan penyabunan, dan bilangan peroksida dengan metode Badan Standarisasi Nasional. Dari hasil penelitian pada ikan patin dengan berat 650-879 gram diperoleh kadar minyak rata-rata 3,827(%W/W), bilangan asam berkisar antara 3,667-19,521 mgKOH/gr, bilangan penyabunan berkisar antara91,319-192,656 mg KOH/gr, dan bilangan peroksida berkisar antara 0,778-17,78 mek/kg.
Analisis Kualitatif dan Kuantitatif Asam Lemak Tak Jenuh Omega-3 dari Minyak Ikan Patin (Pangasius pangasius) dengan Metoda Kromatografi Gas Almunady T. Panagan; Heni Yohandini; Jojor Uli Gultom
Jurnal Penelitian Sains Vol 14, No 4 (2011)
Publisher : Faculty of Mathtmatics and Natural Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (941.197 KB) | DOI: 10.56064/jps.v14i4.204

Abstract

Telah dilakukan analisis kualitatif dan kuantitatif asam lemak tak jenuh Omega-3 pada minyak ikan patin (Pangasius pangasius) dengan menggunakan metode kromatografi gas. Kadar Omega-3 yang diperoleh dari minyak ikan patin yaitu berkisar antara 1,16-12,44%. Dari kadar Omega-3 yang diperoleh dari penelitian ini, ikan patin dapat digunakan sebagai sumber Omega-3 alternatif. Dalam pnelitian ini juga dilakukan karakterisasi minyak ikan patin yang meliputi kadar minyak, bilangan asam, bilangan penyabunan, dan bilangan peroksida dengan metode Badan Standarisasi Nasional. Dari hasil penelitian pada ikan patin dengan berat 650-870 gram diperoleh kadar minyak rata-rata 3,827%, bilangan asam berkisar antara 3,667-19,521 mgKOH/gr, bilangan penyabunan berkisar antara 91,319-192,656 mg KOH/gr, dan bilangan peroksida berkisar antara 0,778-17,78 mek/kg.
Analisis Kualitatif dan Kuantitatif Asam Lemak Tak Jenuh Omega-3, Omega-6 dan Karakterisasi Minyak Ikan Patin (Pangasius pangasius) Almunady T. Panagan; Heni Yohandini; Mila Wulandari
Jurnal Penelitian Sains (JPS) Vol 15, No 3: Juli 2012
Publisher : Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (146.582 KB)

Abstract

Telah dilakukan analisis kualitatif dan kuantitatif asam lemak tak jenuh Omega-3 dan Omega-6pada minyak ikan patin (Pangasius pangasius) dengan menggunakan metode kromatografi gas. Kadar Omega-3yang diperoleh dari minyak ikan patin yaitu berkisar antara 1,16-12,44(%W/W) dan kadar Omega-6 berkisaranantara 12,278-15,961(%W/W). Dari kadar Omega-3 dan dari kadar Omega-6 yang diperoleh dari penelitianini, ikan patin dapat digunakan sebagai sumber Omega-3 dan Omega-6 alternatif. Dalam penelitian ini jugadilakukan karakterisasi minyak ikan patin yang meliputi kadar minyak, bilangan asam, bilangan penyabunan,dan bilangan peroksida dengan metode Badan Standarisasi Nasional. Dari hasil penelitian pada ikan patindengan berat 650-879 gram diperoleh kadar minyak rata-rata 3,827(%W/W), bilangan asam berkisar antara3,667-19,521 mgKOH/gr, bilangan penyabunan berkisar antara91,319-192,656 mg KOH/gr, dan bilangan peroksida berkisar antara 0,778-17,78 mek/kg.
Co-Authors . AKHMALOKA Abd. Rasyid Syamsuri Adik Ahmadi Alfian Hasbi Almunady T. Panagan Almunady T. Panagan Ani Sarah Arini, Feggy Augesti Erisna Ayu Lestari Ayu Safitri Bijak Riyandi Ahadito Budiono Budiono Dasril Basir Dirli Fahmi Rizal Elfita Elfita Elfita Elfita Elfita Elfita Elfita Elfita Elfita Elfita Elfita Elfita Elfita Eliza Eliza Elvi Elvi Esthy Rahman Asih Evi Kaderani Barutu Feggy Arini Ferlinahayati Ferlinahayati Ferlinahayati Ferlinahayati FIDA MADAYANTI Fitri Fitri Fitrya . Fitrya Fitrya Fitrya Fitrya Hary Widjajanti Hermansyah Hermansyah Hermansyah Hermansyah Hermansyah Hermansyah Herwin Purnomo IHSAN IVANTRI Isrowiyatun Daiyah Jasmi Jasmi Jojor Uli Gultom Julinar Julinar Julinar Julinar Juraida Roito Hrp Jurnal Pepadu Lily Restusari Lily Restusari Maghfirahmah Amsyah Putri Marieska Verawaty Maryadi Maryadi Maryadi Maryadi Maryadi Maryadi Melinda Susanti S Melly Wardanis Melsa Nilmalasari Miksusanti Miksusanti Mila Wulandari Mila Wulandari Mita Puspitasari Mudinillah, Adam Muharni Muharni Muharni Muharni Muharni Muharni Muharni Muharni Muharni Muharni Muharni Muharni Muharni Muharni Muharni Muharni Munawar Munawar Nirwan Syarif Nirwan Syarif, Nirwan Nova Yuliasari Novilla Rezka Sjahjadi oktaviani, muthia PINGKAN ADITIAWATI Pramoe Wibowo Putra Setia Aji Candra Rahmi, Junaida Rifdah Nabila Riska Adillah Rivai, M Yunus Rivai, M Yunus Rr. Kusuma Nurin Husna Seni Metasari Seni Metasari Stephani Stephani Suheyanto Suheryanto Susilawati Susilawati Syndi N.K Yeni Aryani Yulia Fitri Yundari, Yundari