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The Carrageenan Dietary Fiber Suplementation in Feed to Improving Blood Lipid Parameters of Hypercholesterolemic Mice Hernawati Hernawati; Wasmen Manalu; Agik Suprayogi; Dewi Apri Astuti
Makara Journal of Health Research Vol 17, No 1 (2013): June
Publisher : Universitas Indonesia

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Abstract

Carrageenan is example of food with high content of fiber. An experiment was conducted to study the effects of carrageenan supplementations on blood lipid parameters of hypercholesterolemic male mice. The experiment were done at animal cages Departemen of Biology Education, Indonesia University of Education and Fisiology Laboratory Faculty of Veteriner Medicine, Bogor Agricultural Institute on August 2011 until March 2012. The experimental mice were assigned into a completely randomized design with 5 treatments i.e., negative control group; normocholesterolemic mice fed with a standard diet, positive control group; hypercholesterolemic mice fed with a standard diet without carrageenan supplementation, treatments group; hypercholesterolemic mice feds with supplemented with 15%, 30%, and 46% carrageenan. The nonsoluble content of dietary fiber in the experimental treatments were 6.92, 8.75, 10.48, 12.27, and 14.05%, respectively. The parameters measured were body weight, cholesterol levels of blood serum, liver and feces, triglyceride, high density lipoprotein (HDL), low density lipoprotein (LDL) and glucose levels. Supplementation of carrageenan as a source of dietary fiber increased serum HDL concentrations, and decreased body weight, serum cholesterol, triglyceride, and LDL concentrations of hypercholesterolemic male mice without a significant effect on serum glucose consentrations. Hypercholesterolemic mice fed with a supplemented with 46% carrageenan decreased body weight by 7.99%, total serum cholesterol by 18.78%, triglyceride by 17.53%, LDL by 71.33%, and increased HDL by 15.59−20.47%. Carrageenan supplementation reduce liver cholesterol levels by 38.46% and increased cholesterol excretion through feces by 57.07%. Supplementation of 46% carrageenan in hipercholesterolemic fed is effective in improving blood lipid parameters of hypercholesterolemic male mice.
Uji Hayati Lima Jenis Minuman Kemasan Gelas dengan Hewan Uji Daphnia Magna dan Mencit Jantan (Mus musculus) Hertien Koosbandiah Surtikanti; Hernawati .; Diah Frisda; Muhamad Taufiq Hidayah
Jurnal Biodjati Vol 3, No 1 (2018): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v3i1.2348

Abstract

Lima jenis minuman yang terpilih yaitu panther, meico, teh zeggar, degan, dan teh bukit sudah dilakukan uji hayati. Salah satu untuk pengujian kualitas minuman tersebut, yaitu uji hayati dengan menggunakan neonate Daphnia magna  (berumur <24 jam) dan mencit jantan (Mus musculus). Pengujian menggunakan D. magna, dilakukan dalam dua tahap yaitu Range Finding test (RFT) dan Range Definitive Test (RDT), sementara uji hayati mencit jantan galur Deutschland Denken Yoken (DDY) dilakukan dengan Rancangan Acak Lengkap, dua faktorial dengan 18 kelompok perlakuan. Pengambilan sampel darah mencit jantan diambil pada bagian ekor hewan dan diperiksa dengan menggunakan metode strip dan glucometer autocheck. Hasil RFT menunjukan 50% kematian neonate D. magna terdapat pada pengenceran 10-100%. Hasil analisis menunjukkan nilai LC50-24h untuk minuman Panther (P), Meico (M), Teh Bukit (B), Teh Zegar (Z) dan Degan (D). berturut-turut adalah 39,99; 51,5; 40,9; 40,93; dan 49,74%. Hasil uji hayati menggunakan mencit jantan, terjadi kenaikan berat badan yang signifikan pada saat diberi Z, D dan B. Kenaikan kadar gula darah mencit jantan signifikan pada waktu perlakuan selama dua dan tiga minggu dengan P, M, B, Z dan D. Hal ini disebabkan kadar gula minuman dapat meningkatkan kadar gula darah. Dapat disimpulkan bahwa kelima sampel minuman tersebut bersifat toksik, karena nilai LC50 kurang dari 50% (hasil uji D. magna) dan dapat meningkatkan kadar gula darah pada mencit jantan.
Analisis kemampuan klasifikasi mahasiswa pendidikan biologi pada pembelajaran biosistematik hewan Hernawati Hernawati; Yayan Sanjaya; Fransisca Sudargo; Any Aryani; Zamzam Nursani; Dita Puspitasari; Try Kurniawan; Rahadian Deden Juansa
Assimilation: Indonesian Journal of Biology Education Vol 4, No 1 (2021): March 2021
Publisher : Department of Biology Education, Universitas Pendidikan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17509/aijbe.v4i1.34821

Abstract

This study aims to analyze the classification ability in animal biosystematics learning as the basis for making numerical taxonomies in biology education students. The method in this research consists of qualitative and quantitative analysis. Descriptive method is used for qualitative analysis and correlation method for quantitative analysis. The mixed methods strategy uses simultaneous triangulation because both qualitative and quantitative data are collected at the same time, then compared to identify convergence of differences or combinations of data. The number of students was 57 people who were divided into 11 groups. Ability classification is determined based on indicators that refer to science process skills. The classification ability indicator is made into six indicators, where each indicator is broken down into four sub indicators. The results of the analysis show that the classification abilities of the six indicators are: (1) recording each observation separately 89.04%, (2) looking for similarities and differences of 66.23%, (3) contrasting the features of 57.89%, (4) comparing of group characteristics 67.32%, (5) determining the basis for grouping 73.46%, and (6) connecting the results of observations 76.32%. The ability of individuals to make classifications in animal biosystematic learning reached 71.72%, while in groups it was 71.98%. Based on the results above, it can be concluded that the classification ability of participants in animal biosystematic learning is good with the medium category, this can support in making numerical taxonomy.
Uji Mikrobiologi Biskuit Dengan Penambahan Tepung Kulit Pisang Hernawati, Hernawati; Aryani, Any; Shintawati, Rita
Life Science Vol 7 No 2 (2018): November 2018
Publisher : Universitas Negeri Semarang

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Abstract

Permintaan terhadap produk makanan kesehatan seperti makanan bebas gula, rendah kalori, dan kaya serat semakin meningkat. Telah dibuat biskuit berbahan tepung kulit pisang yang memiliki kandungan serat pangan yang cukup tinggi. Tujuan penelitian yaitu mengidentifikasi jumlah mikroorganisme pada biskuit tepung kulit pisang. Metode pengujian mikrobiologi sampel makanan dilakukan yaitu uji TPC (Total Plate Count) untuk menghitung bakteri, Most Probability Number (MPN) untuk menghitung jumlah Escherchia coli, Salmonella sp., dan hitungan cawan untuk menghitung jumlah Staphylococcus sp. serta kapang/khamir. Analisis data mengacu pada persyaratan makanan yang sudah ditetapkan sesuai Standar Nasional Indonesia (SNI 01-2897-1992). Hasil penelitian menunjukkan bahwa pengujian mikroba biskuit tepung kulit pisang normal tidak melebihi ketentuan Standar Nasional Indonesia (SNI) yang mempersyaratkan maksimal sebesar 1,0x104 koloni/g bakteri. Uji Salmonella sp. diperoleh hasil negatif. Hasil uji kandungan E. coli kurang dari 3/g. Hasil uji kandungan Staphylococcus aureus sebanyak <1,0x101 koloni/g. Hasil uji jumlah kapang/khamir kurang dari 1,0x101 koloni/g. Kesimpulan hasil identifikasi mikrobiologi biskuit tepung kulit pisang dinyatakan dalam kategori normal sesuai Standar Nasional Indonesia (SNI, 2009) dan BPOM (2009), sehingga dapat dikonsumsi oleh masyarakat sebagai makanan kesehatan. Demand for health food products such as sugar-free foods, low in calories and fiber-rich foods is on the rise. It is known that made biscuits made from banana peel flour is known to have high dietary fiber content. The aim of this research was to identify the number of microorganisms on the biscuits with addition banana peel flour. Microbiological test method of food samples was done by TPC (Total Plate Count) test to calculate bacteria, Most Probability Number (MPN) to calculate the amount of Escherchia coli, Salmonella sp., and plate count to calculate the amount of Staphylococcus sp. and mold/yeasts. The results of data analysis refers to food requirements that have been established according to Indonesian National Standard (SNI 01-2897-1992). The results showed that the testing of microbial biscuits of banana peel flour did not exceed the provisions of Indonesian National Standard (SNI) which required a maximum of 1.0x104 colony/g bacteria. Salmonella sp. obtained negative results. The results of Escherchia coli content test less than 3/g. The results of the Staphylococcus aureus content test were <1.0x101 colony/g. The test result of the amount of mold/yeast is less than 1.0x101 colony/g. The conclusion of microbiology identification result stated that biscuit of banana peel flour was normal category according to Indonesian National Standard (SNI, 2009) and BPOM (2009), so it can be consumed by society as health food.
STUDI TENTANG KETERAMPILAN PEMBENTUKAN KLAIM MENGENAI ISU SOSIO-SAINTIFIK SISWA SEKOLAH MENENGAH ATAS PADA KELOMPOK BUDAYA SUNDA Bambang Ekanara; Nuryani Y Rustaman; Hernawati Hernawati
BIODIDAKTIKA: JURNAL BIOLOGI DAN PEMBELAJARANNYA Vol 11, No 2 (2016)
Publisher : Universitas Sultan Ageng Tirtayasa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30870/biodidaktika.v11i2.1585

Abstract

ABSTRACTThis case study purposes to investigate Sundanese high school student 11th grade argumentation skills about socio-scientific issue based on gender and family caring pattern. Specifically, this qualitative research explores the quality of Sundanese student’ claim-forming and evidence-supplying in an argumentation process. Research instrument were given to 44 year-11 students, argumentation worksheets were used to get student’ written argumentation skills (WAS) data; semi-structured interview were used to get student’ oral argumentation skills (OAS) data; questionnaire and interview were used to get family caring pattern and family gender awareness data; furthermore, field note were used as data triangulation tool. Student’ argumentation skills were determined by justification of argument based on four-scale rubric. Inductive analysis was used to examine the data. Most Sundanese student had capable to form an argument with a simple justification; student’ OAS better than WAS relatively; and other  result of this study shows that no argumentation skills significance difference between boys and girl. The findings suggest several factors influencing Sundanese student’ argumentation skills such as freedom of opinion-forming in family life, student’ role in family, the language ability to articulate ideas, and  cultural influences. Methodological issues arising from the study and implications for teaching and assessment are discussed.