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All Journal Microbiology Indonesia Jurnal Keperawatan Soedirman Kesmas Indonesia: Jurnal Ilmiah Kesehatan Masyarakat Biosaintifika: Journal of Biology & Biology Education Indonesian Journal of Obstetrics and Gynecology (Majalah Obstetri dan Ginekologi Indonesia) Journal of Bionursing

. HERNAYANTI

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Author-ID : 280782

Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Suplemen antioksidan VCO (Virgin Coconut Oil) yang diperkaya dengan Zn bagi penderita Candidiasis Vagina WINARSI, H; HERNAYANTI, HERNAYANTI; PURWANTO, A.
Indonesian Journal of Obstetrics and Gynecology Volume. 32, No. 3, July 2008
Publisher : Indonesian Socety of Obstetrics and Gynecology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (163.523 KB)

Tujuan: Mengetahui aktivitas SOD, katalase, dan GSH-PX limfosit, serta kadar MDA plasma penderita Candidiasis Vagina yang diintervensi VCO yang diperkaya dengan Zn selama 2 bulan. Rancangan/rumusan data: Penelitian eksperimental dengan rancangan acak lengkap. Analisis data menggunakan anova single faktor, dilanjutkan dengan uji Duncan bila terdapat signifikansi. Tempat: Sampel sekret diperoleh dari RS Sinar Kasih, tempat prostitusi (Gang Sadar), Puskesmas, dan praktik dokter di Purwokerto. Pengujian aktivitas enzim antioksidan dan kadar MDA di laboratorium Klinik Mediko Purwokerto. Bahan dan cara kerja: Penelitian dilakukan mulai Oktober 2006 sampai April 2007. Sebanyak 30 responden dipilih berdasar kriteria seperti berikut, sekret vagina mengandung C. albicans lebih dari 105 cfu/ml, sukarela, sehat, bersedia menandatangani informed consent, dan tinggal di Purwokerto. Responden dibagi dalam 3 kelompok; kelompok A sebanyak 10 orang diintervensi dengan VCO yang diperkaya Zn dengan dosis 2 sdm/hari; kelompok B sebanyak 10 orang diintervensi dengan dosis 1 sdm/hari; dan kelompok C sebanyak 10 orang sebagai kontrol. Pengambilan sampel darah dilakukan 3 kali yaitu pada baseline, 1 dan 2 bulan setelah intervensi. Uji aktivitas enzim antioksidan dan kadar MDA menggunakan spektrofotometer. Hasil: Setelah 2 bulan intervensi, aktivitas enzim SOD meningkat dari 87,32 menjadi 348,70 unit/mg protein (p=0,00016), katalase meningkat dari 20,42 menjadi 40,12 Unit/mg protein (p=0,031); demikian pula glutation peroksidase limfosit juga meningkat dari 5,77 menjadi 10,64 Î¼mol/gr protein (p=0,036). Sebaliknya kadar MDA plasma menurun dari 1.858,86 menjadi 1.214,36 pmol/ml (p=0,019). Kesimpulan: Intervensi VCO yang disuplementasi dengan Zn kepada penderita Candidiasis Vagina berpotensi sebagai antioksidan. Selanjutnya, mereka disarankan mengkonsumsi suplemen dengan dosis 1 sdmx1. [Maj Obstet Ginekol Indones 2008; 32-3: 177-82] Kata kunci: SOD, katalase, GSH-PX, MDA, candidiasis vagina.

Effects of Ganoderma lucidum Extract on Diabetic Rats Ratnaningtyas, Nuniek Ina; Hernayanti, Hernayanti; Andarwanti, Suci; Ekowati, Nuraeni; Purwanti, Endang Sri; Sukmawati, Dalia
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Diabetes mellitus (DM) is a metabolic syndrome which occurs when insulin is insufficiently produced or insulin cannot well serve its function. Diabetes is marked with increase in blood glucose level followed by increase in glycosylated hemoglobin level and decrease in insulin level. This research aims to examining the effect of Ganoderma lucidum extract on the blood glucose, insulin and glycosylated hemoglobin (HbA1c) level of diabetic white rat and determining the most effective dose of extract to be a diabetic agent. This research was experimentally conducted by employing Completely Randomized Design (CRD) with 6 treatments and 4 repetitions. The treatment groups consisted of healthy rats group (K1), rats with diabetes as negative control (K2), diabetic rats with the administration of metformin 45 mg/kg BW as the comparison (K3) and diabetic rats with the administration of G. lucidum extract with dose of 250, 500 and 1000 mg /kg BW (K4, K5 and K6 respectively). Blood glucose level examination was conducted after the alloxan induction with single dose of 125 mg/kg BW by intraperitoneal injection. The results show that mushroom G. lucidum extract administration with dose of 1000 mg/kg BW (K6) is the best dose to be an anti-diabetic agent. The benefit of the research is developing anti-diabetic agent from herbal resources.

Antidiabetic Potentials of Button Mushroom (Agaricus bisporus) on Alloxan-Induced Diabetic Rats Ekowati, Nuraeni; Yuniati, Nilasari Indah; Hernayanti, Hernayanti; Ratnaningtyas, Nuniek Ina
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Button mushrooms (Agaricus bisporus) is an edible mushroom that is most widely cultivated in the world. It contains bioactive compounds that might provide beneficial effects on diabetes mellitus patient. The study aimed to determine the effects of A. bisporus administration on the blood glucose, and malondyaldehyd (MDA) levels as well as superoxide dismutase (SOD) activity of alloxan-induced diabetic rats. This study was also conducted to determine the secondary metabolites produced by A. bisporus. The method used was experimental methods with Completely Randomized Design. A. bisporus extract at the doses of 250, 500 and 750 mg/kg body weight (BW) per day were orally applied to alloxan-induced diabetic rats for a period of 14 days after the rats became diabetes. The results showed that the extract of A. bisporus could decrease blood glucose, and MDA levels as well as increase SOD activity (p < 0.05). A. bisporus extract 500 mg/kg BW is the most effective dose to be used. Based on Thin Layer Chromatography (TLC) test, it was known that secondary metabolites produced by A. bisporus are flavonoids, alkaloids, terpenoids and saponins. A. bisporus has potential as an antidiabetic through the ability to decrease blood glucose, and MDA levels, as well as increase SOD activity in diabetic rats. This research is able to provide information about the antidiabetic potential of A. bisporus extract so that it can be used as an alternative natural antidiabetic agent and can be applied in the community with ease and in a more controlled industrial scale.

Antioxidant Effect of Chlorella vulgaris on Physiological Response of Rat Induced by Carbon Tetrachloride Hernayanti, Hernayanti; Simanjuntak, Sorta Basar Ida
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 1 (2019): April 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i1.16393

Chlorella vulgaris is an algae with high nutrition content. Carbon tetrachloride (CCl4) is a hepatotoxic chemical.The aims of this study were to determine the effect of C. vulgaris extract on the physiological response of liver such as MDA, SOD and GPx activity on rat after induced by CCl4 exposure as well as to determine the effective dose of C. vulgaris extract as antioxidant that can neutralize CCl4 exposure. This research was conducted experimentally with Completely Randomized Design that consists of 6 treatment and 5 times repetition. The doses of C. vulgaris extract used were 3, 4, and 5 mg per /100 g of rat’sbody weight (BW).The administration of C. vulgaris extract was performed within 30 days, while the CCl4 (0.25 ml/100 g BW) was administered orally on the day 9, 12, 16, 19, 23, and 26. Parameters measured were levels of MDA, SOD and GPx of rat blood serum.The results showed that the administration of C. vulgaris extract can inhibit lipid peroxidation indicated by decrease in MDA activities and oxidative stress by increasing SOD and GPx activity. In conclusion, 5 mg/100 g BW of C.vulgaris extract is an effective dose to be uses as endogenous antioxidant to protect the liver cell from damage caused by CCl4exposure. The benefit of C. vulgaris as a supplement for antihepatotoxin in humans.

The Cytotoxic and Apoptosis Effects of Chloroform Extracts of Auricularia auricula on Cervical Cancer Cells Hikam, Arif Rahman; Ekowati, Nuraeni; Hernayanti, Hernayanti
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 1 (2019): April 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Auricularia auricula is an edible mushroom cultivated in Indonesia that has been known to have potential properties of bioactive compounds than can be used for medicinal purpose. This study aimed to examine the cytotoxic and apoptosis effect of chloroform extract of A. auricula on cervical cancer cells in vitro. The research design was in vitro experimental research. Cytotoxic tests was using an MTT [3-(4,5-dimetiltiazol-2-yl)-2.5-diphenyl tertrazolium bromide] assay and apoptosis test was using double staining method. Test of bioactive compounds was carried out using GCMS. Cytotoxic effect were analyzed by linear regression and apoptosis test was analyzed descriptively. Chloroform extract of mycelium A. auricula showed the best results with IC50 = 264.87 µg/ml. An important finding obtained after the double staining process was that chloroform extract of A. auricula can induce HeLa cells death by apoptosis. GCMS test results showed that the extracts containing limonene and piperidinone which are the anticancer bioactive compounds. In conclusion, the chloroform extracts of A. auricula has the potential to inhibit the growth of cervical cancer cells. The benefit of this study are expected to provide information about the anticancer potential of extract of A. auricula against cervical cancer cells, thus contributing to the development of alternative anticancer treatments from natural product.

EFFECT CADMIUM EXPOSURE ON RENAL FUNCTION OF WELDING WORKERS hernayanti, hernayanti; Santoso, Slamet; Lestari, Sri; Prayoga, Lucky; Kamsinah, Kamsinah; Rochmatino, Rochmatino
Kesmas Indonesia: Jurnal Ilmiah Kesehatan Masyarakat Vol 11 No 1 (2019): Jurnal Kesmas Indonesia
Publisher : Jurusan Kesehatan Masyarakat dan Fakultas Ilmu-Ilmu Kesehatan Universitas Jenderal Soedirman

ABSTRACT Cadmium is one of the heavy metals that has nephroxic effect and  dangerous to  human health, especially welding workers. If cadmium enters the body will bind to metallotionein protein.  This process can increase the formation of free radical compounds, thereby induced lipid peroxidation which damage kidney organ and may cause impaired the renal function. Decreased renal function is characterized by an increase of ?2-Microglobulin and creatinine level. The aim of this study are to know effect of Cd exposure to renal function indicated by increasing of Cd, creatinine and ?2-M level. Research design used case control. Research subjects consist of thirty welding workers in Purwokerto with a period of working more than 3 years as case subjects and control subjects come from Pamijen village Baturaden.  Data of Cd, creatinine and ?2-M level were analyzed by t independent. Result of this research showed that in case subjects occur an increase of Cd, creatinine and ?2-M level and have higher level compare with control subjects.It can be concluded that Cd exposure has an effect on renal disfunction which indicated by increasing  blood Cd level, creatinine and ?2-M level.             

PENURUNAN TOKSISITAS KADMIUM DENGAN KELATOR ALAMI PEGAGAN (CENTELLA ASIATICA) DITINJAU DARI KADAR MALONDIALDEHID (MDA) DAN SUPEROKSIDA DISMUTASE (SOD) Hernayanti, Hernayanti; Lestari, Sri
Journal of Bionursing Vol 2, No 1 (2020)
Publisher : Fakultas Ilmu-ilmu Kesehatan Universitas Jenderal Soedirman

Cadmium (Cd) is a heavy metal that is toxic to humans. Cadmium will bind to metalotionin in the liver to form Cd+MT bonds, which triggers the formation of free radicals and causes oxidative stress. Oxidative stress characterized by elevating of MDA and decreasing of antioxidant enzyme such as SOD. Gotu kola(Centella asiatica) contains active compounds medecassoside that can function to chelate Cd, decreasing MDA level caused by Cd-Mt bounded and generate SOD level.The aim of this study is to know the effect of Pegagan as natural chelator for Cd poisoning and as antioxidant. Twenty four rats were used in this research and divided into six groups with four replications. Group 1 (C1) as healthy. Group 2 (C2) was only given 14 mg/200 gBW of CdSO4 for 35 days. Group 3, 4, 5 and 6 were given CdSO4 14 mg / 200kgBB and gotu kola extract dose of 20 mg/200gBB, 40 mg/200gBB, 60 mg/200gBB, 80 mg / 200gBB, for 21 days. The parameters studied were blood MDA and SOD level. Measuring parameters were done on the 36th day after administration of Centella extract. Data were analyzed by Anova and followed by Duncan test The results showed that Centella all doses can reduce MDA level and increase SOD level. A dose of 40mg / 200gBB Centella extract has been effective in reducing MDA level as well as increasing SOD level. It can be concluded that dose of 40mg/200gBB can use as a natural chelator of Cd and as antioxidant for Cd poisoning.

Blood Hematological and Biochemical Parameters of Osteochilus vittatus with Spirulina platensis Supplementation in Biofloc System Rijal, Muhammad Azharul; Susanto, Susanto; Simanjuntak, Sorta Basar Ida; Hernayanti, Hernayanti; Sukardi, Purnama
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.20936

Spirulina platensis is a microalgae that contains nutrients such us iron and phycocianin higher than other microalgae. Research on suplementation of S. platensis in Osteochilus vittatus cultured in biofloc system has never been done. The aim of this study was to determine the blood hematological and biochemical parameters of O. vittatus fed with S. platensis supplementation and maintained in biofloc system. The study was conducted experimentally with Completely Randomized Design, four treatments and five replications. O. vittatus were fed with S. platensis level 0, 2, 4, and 6 g.kg-1 for 56 days. Blood hematological and biochemical parameters were measured on days 0 and 56. Values of those parameters were then analyzed using ANOVA with a confidence level of 95%. The result showed that the highest red blood cells, white blood cells, and hemoglobin counts were 1.57x106ÂÂÂÂ cell.mm-3, 2.37x105 cell.mm-3, and 6.77 g.dl-1 respectivley, while the highest hematocrit value was 17.5 %. The highest total protein, albumin, and globulin in blood were 7.96 g.dL-1, 4.31 g.dL-1, and 3.79 g.dL-1 respectively, and the best for ratio A/G was 1.14. S. platensis suplementation level of 4g.kg-1 feed was the most optimum level (P<0.05). Cultivation of fish with S. platensis supplementation in feed can increase fish health indicated by the hematological and biochemical parameters of the blood and the fish maintenance in biofloc systems can improve water quality. The benefit of this research is to make a good condition for cultivation and efficiently used not only water but also feed.

Efek Daun Alpukat (Persea Americana M.) dan Daun Kelor (Moringa Oleifera L.) terhadap Peningkatan Kadar HDL Pada Model Tikus Putih Hiperlipidemia Handayani, Sri; Saryono,; Hernayanti,
Jurnal Keperawatan Soedirman Vol 12, No 1 (2017)
Publisher : Jurusan Keperawatan FIKES UNSOED

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jks.2017.12.1.717

Hyperlipidemia is a lipid metabolism disorder which is characterized by decreasing of High Density Lipoprotein (HDL) level. Avocado (Persea americana) and Kelor (Moringa oleifera) leaves can be proposed for increasing HDL level. This research aimed to determine the steeping combination of avocado and kelor leaves effect to against HDL level for hyperlipidemia rat model. This research used true experimental pre and post test with control design. Amount of 36 rats were divided into negative control (A), single dose of Persea leaves steeping 36mg/200gramBB (B), combination of 36mg/200gramBW Persea leaves and 54 mg/200gramBW Moringa leaves (C), combination of 18mg/200gramBB Persea leaves and 27 mg/200gramBW Moringa leaves (D), single dose of Moringa leaves steeping 54mg/200gramBW (E), and simvastatin 0,018 mg/day (F). Data were analyzed by ANOVAÃ‚Â test and continued by Duncan post-hoc test. ANOVA tests showed there are significant differences bethween group (p = 0.008). Duncan post hoc test showed that the combination group and simvastatin control group are equally strong to increase HDL levels. The steeping combination of Persea and Moringa leaves has an effect to increasing HDL level.

A Supplement Based on Zn-Enriched Virgin Coconut Oil as an Antifungal agent for Vaginal Candidiasis Patients . HERY WINARSI; . HERNAYANTI; AGUS PURWANTO
Microbiology Indonesia Vol. 2 No. 2 (2008): August 2008
Publisher : Indonesian Society for microbiology

This research was conducted to investigate the amount of Candida albicans in vaginal secretion of Vaginal Candidiasis patients administered with Zn-enriched virgin coconut oil. Thirty respondents were selected based on several criteria as follows: the number of C. albicans colonies in the vaginal secretion was more than 105 cfu.ml-1, voluntary, healthy, willing to sign the informed consent and resided in Purwokerto. In Group A, 10 women were administered 2 tablespoons per day of Zn-enriched virgin coconut oil. In Group B, 10 women were administered 1 tablespoon per day of Zn-enriched virgin coconut oil; and in Group C, 10 women served as control group. Vaginal secretions were taken 3 times, before intervention (at baseline time), at 1 month and 2 months after intervention. Samples were taken by collecting vaginal secretions from the vaginal proximal region using a sterile cotton bud, which was then put into a tube containing sterile carrier media. The vaginal secretions were tested for the number of total C. albicans using Pour Plate Method. Two months after treatment, the number of colonies decreased from 4.4x106 to 2.5x106 cfu ml-1 (p=0.03) in Group A. There was no significant difference in the number of colonies between Group A and Group B, the number of C. albicans colonies was still above the normal range. Therefore, the recommended dosage of intervention with Zn-enriched virgin coconut oil is one tablespoon a day.

Co-Authors . HERY WINARSI A. Purwanto A.A. Ketut Agung Cahyawan W Agung Saprasetya Dwi Laksana, Agung Saprasetya Dwi Agus Purwanto Ahmad Hamim Sadewa Anak Agung Gede Sugianthara Andarwanti, Suci Bambang Hariono Dalia Sukmawati Endang Sri Purwanti Endang Widyastuti Handayani, Sri I Gusti Wayan Murjana Yasa Kamsinah Kamsinah Lucky Prayoga Nuniek Ina Ratnaningtyas Nuraeni Ekowati, Nuraeni Purnama Sukardi Rijal, Muhammad Azharul Rochmatino Rochmatino, Rochmatino Saefuddin Aziz Saryono, Siti Hidayati Sorta Basar Ida Simanjuntak Sri Lestari Subagus Wahyuono Sukarti Moeljopawiro Susanto Susanto WINARSI, H Yuniati, Nilasari Indah

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