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Efek Perlindungan Susu Kedelai (Glycine max) Ultra High Temperature (UHT) Terhadap Lambung Mencit Ginong Pratidina Wijnaputri; Endang Listyaningsih; Jarot Subandono
Nexus Biomedika Vol 1, No 2 (2012): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: White Soybean (Glycine max) Milk Ultra High Temperature (UHT) is potential to protect gaster because it contains flavonoid. This study aimed to investigate the protective effect of white soybean milk UHT on mices gaster due to aspirin. Higher dose of the milk reduces the damaged on gastric mucous induced by aspirin Methods: This was a labroratory experimental research with posttest only controlled group design conducted in in Histology Laboratory of Sebelas Maret University. The samples were thirty five mice divided into 5 groups. Negative control group (KN) was given normal food and aquadest, positive control group (KP) was given aspirin dose 2,275 mg/20g Body weight (W), dan first treatment group (P1) was given cimetidine dose 0,78 mg/20g W second treatment group (P2) and third treatment group (P3) was given soybean milk UHT dose 0,7ml/20 g W and 1,4 ml/20 g W. All treatments for KN, KP, P1 , P2, P3 was given in 10 days. Aspirin was given to KP, P1 , P2, P3 with dose 2,275 mg/20g weight of mice on day 8, 9 and 10. to evaluate the level of damage based on histologic appearance, gastric staining was performed using Haem eos. Results: Kruskal-Wallis test result showed significant result among four groups. Mann-Whitney showed significant result for KN compared to KP,P1,P2 , P3 and KP compared to P1, P2 and P3 ; while P1 compared to P2, P3 and P2 compared to P3 had no significant result. Conclusion: White Soybean (Glycine max) Milk Ultra High Temperature(UHT) protect mices gastric from gastritis. However, higher dose of White Soybean (Glycine max) Milk Ultra High Temperature(UHT) does not increase the protective effect of white soybean milk UHT on mices gaster due to aspirin. Keywords: White Soybean (Glycine max) Milk Ultra High Temperature(UHT), gastric protection, gastritis, aspirin.

Efek Pemberian Sari Buah Belimbing Manis (Averrhoa carambola Linn.)terhadap Kerusakan Histologis Hepar Tikus Putih(Rattus norvegicus) yang Diinduksi Parasetamol Handayani Putri Cahyaningrum; Endang Listyaningsih; Selfi Handayani
Nexus Biomedika Vol 2, No 1 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: Star fruit (Averrhoa carambola Linn.) juice contains flavonoids, epicatechins, proanthocyanidins, and tanins that can protect liver from free radicals. The objectives of this research were to know the effect of star fruit (Averrhoa carambola Linn.) juice to liver histological damage of albino rat induced by paracetamol. Methods: This was laboratory experimental research with post test only controlled group design. Samples were 28 male rats from Wistar type, 2-3 months old and + 200 g of each weight. Samples were divided into 4 groups of 7 rats each. Sampling technique used in this research was incidental sampling. The control group (K) and the first treatment group (P1), rats were administered aquadest for 14 days. The second treatment group (P2), rats were administered star fruits juice with the concentration of 50% 2ml/200 g body weight of rat and the third group (P3), rats were administered star fruits juice with the concentration of 100% 2ml/200 g body weight of rat for 14 days. Paracetamol was administered to groups of P1, P2, and P3 on the 12th, 13th, and 14th day. On the 15th day, rats were sacrificed and their livers were made for preparation with paraffin block method which were stained HE. Liver histological features were assessed based on quantifying of pyknosis, karyorhexis, and karyolysis. Data were analyzed using the One-Way ANOVA test (? = 0.05) and continued with Post Hoc Multiple Comparisons (LSD) test (? = 0.05). Results: Statistically, the One-Way ANOVA test showed that there were significant differences of liver damage score between K P1, K P2, K P3, P1 P2, P1 P3, and P2 P3. Conclusion: Star fruit juice decreased the liver histological damage of albino rat (Rattus norvegicus) induced by paracetamol. Key words: star fruit juice, paracetamol, liver histological damage, albino rat

Efek Proteksi Biji Mahoni (Swietenia mahagoni Jacq.) terhadap Kerusakan Hepar Mencit yang Diinduksi Parasetamol Dahniar Endahfuri; Endang Listyaningsih; Yulia Sari
Nexus Kedokteran Translasional Vol 1, No 1 (2012): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Background: Seedof Swietenia mahagoni Jacq. contains alkaloids, saponins, flavonoids, triterpenoids, steroids, and tannins which were thought to protect liver from free radicals.In present study, Swietenia mahagony Jacq.seed extract was evaluated for its protective effect on paracetamol-induced liver damage in mice. Methods:To evaluate thehepatoprotectiveeffects of Swietenia mahagony Jacq. seed extract, 28 male Swiss webster micewere equallydividedinto fourgroups. Animals of group I (K) and group II (P1) were given aquadest for 14 days. Group III (P2) received 14 mg/25 g body weight of mice of Swietenia mahagony Jacq. seed extract and the last group (P3)received 28 mg/25 g body weight of mice of Swietenia mahagony Jacq. seed extract for 14 days.Paracetamol was given to groups of P1, P2, andP3.Mice were sacrificedon the 15th day and histological preparation was made to evaluate histological damage on liver. Liver histological features were assessed by counting the number of hepatocyte on centrolobuler zone undergoing pyknosis, karyorhexis, and karyolysis. Data were analyzed using the OneWay ANOVA test (? = 0.05) and then were continued with Post Hoc Multiple Comparisons (LSD) test (? = 0.05). Results:The mean liver histological damage score was significantly higher on group of P1=60.71+7.521, whilegroup of K= 9.86+1.574 as the less.P2grouphad fewer numbers of liver histological damage (32.57+2.573) compared to the P3group(43.57+3.101). The results of OneWay ANOVA and LSD test showed that there was significant difference between the four groups (p = 0.000). Conclusions:Swietenia mahagony Jacq.seed extract showed protective effect against the hepatotoxicity induced by paracetamolin mice.Swietenia mahagony Jacq.seedextract with the dose of 14 mg/25 g bodyweight of mice showed a higher hepatoprotective effect than the dose of 28 mg/25 g body weight of mice. Keywords:Swietenia mahagony Jacq. seed, liver damage,paracetamol

The Effect of Mango (Mangifera indica L.) Juice on Paracetamol Induced Liver Cell Damage in Mice (Mus musculus) Samiaji Abbas Ras; Endang Listyaningsih; Yulia Sari
Nexus Kedokteran Translasional Vol 3, No 1 (2014): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Background: Mango (Mangifera indica L.) contains beta-carotene and vitamin C which were thought to protect the liver from free radicals. In present study, Mango (Mangifera indica L.) juice with certain dose was evaluated for its protective effect on paracetamol induced liver damage in mice. Methods: This study used 28 male Swiss Webster mice were equally divided into four groups. Animals of Control Group (KK) were given aquadest and Treatment Group I (KP1) received paracetamol for 3 days. Treatment Group II and III (KP2 and KP3) not only received paracetamol, but also received multilevel doses of Mango (Mangifera indica L.) juice (0,2 mg and 0,4 mg) for 14 days. Mice were sacrificed on the 15th day and histological preparation was made to evaluate histological damage on liver. Liver histological features were assessed by counting the number of hepatocyte on centrolobuler zone undergoing pyknosis, karyorhexis, and karyolysis. Data were analyzed using the One Way ANOVA test (? = 0.05) and the Post Hoc Multiple Comparisons (LSD) test (? = 0.05). Results: The mean liver histological damage score was significantly higher on group of KP1= 61,87.991, while group of KK= 26,214.828 as the least. KP3 group had fewer numbers of liver histological damage (40,097.635) compared to the KP2 group (37,815.653). The results of One Way ANOVA test showed significant difference between the five groups (p = 0.000) and the results of LSD test showed significant differences between each other group (p < 0.005). Conclusion: Mango (Mangifera indica L.) juice showed protective effect against the hepatotoxicity induced by paracetamol in mice. Mango (Mangifera indica L.) juice with the dose of 0,4 mg/20 g body weight of mice showed a higher hepatoprotective effect than the dose of 0,2 mg/20 g body weight of mice. Keywords: Mango juice, paracetamol, histologic liver damage.

Efek Nefroprotektor Ekstrak Daun Kemangi (Ocimum sanctum) Terhadap Kerusakan Sel Ginjal Mencit (Mus musculus) Yang Diinduksi Parasetamol M Abdul Basith; Endang Listyaningsih; Selfi Handayani
Nexus Biomedika Vol 1, No 2 (2012): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: Today, consuming paracetamol as an analgesic antipyretic drugs has increased. Paracetamol which used in inappropriate dose has bad effect to our body, such as nefrotoxic. Basil leaf extract has antioxidant as a protection of free radicals and reducing NAPQI which produced by paracetamol. The objectives of this research are to know the nefroprotector effect and this research will shown the multilevel dose of basil leaf extract as a nefroprotector in the renal cell damage induced by parasetamol. Methods: This was experimental laboratory with the post test only controlled group design. Sample group consisted of male mice Swiss Webster, 2-3 month, + 20 g. Samples divided into 4 groups, each group has seven mice. Mice for control group (K) and the first treatment group (P1) were given aquades for 14 days. The second treatment group (P2) will be given basil leaf extract dose I for 14 days. The third treatment group (P3) will be given basil leaf extract dose II for 14 days. Paracetamol will be given to P1, P2, and P3, with dose 0,1 ml/20 gr weight of mice on the day 12, 13, and 14. Finally on day 15th, mice are sacrificed with neck dislocation then the renal of mice was made preparations with paraffin blocks methods and Hematoxillin Eosin staining. Renal cell damage observed and counted a mount of scored on renal histological karyopyknosis, karyorrhexis, and karyolysis. Data are analized by One-Way ANOVA test (?= 0,05) and continued by Post Hoc Multiple Comparisons test (LSD) (? = 0,05). Results: Result of One-Way ANOVA shown that there was a significant of degree between 4 groups. Result of LSD method there was a significant of degree between K-P1, K-P2, P1-P2, and P1-P3 groups; and also it wasnt a significant of degree between K-P3 and P2-P3. Conclusions: The basil leaf extracts was able to decrease the renal cell damaging of mice and the increase of basil leaf extracts dose followed by the increase of protection effect to the renal cell damaging of mice which is induced by paracetamol. Keywords: basil leaf extract, paracetamol, renal cell damage.

The Effect of Soy Bean (Glycine max) to mice (Mus musculus) Renal Cells Histophatology Induced with Reused Palm Oil miftah nurizzahid prabowo; Endang Listyaningsih; Zulaika Nur Afifah
Nexus Biomedika Vol 4, No 2 (2015): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: The reused palm oil is usually used by people to cook especially for cooking food. In the society the palm oil is usually used by deep frying method. This methode usually destroys the chemical chain in the palm oil.As results from this deep frying methode many toxic and free radical are formed, this can be dangerous for human body. Soy bean (Glycine max) one of food that contains fitoestrogen and the descents isoflavon. The isoflavone will protect human body from free radicals and has antiinflammatory actitivity. Method: This study was an experimental laboratory using the posttest only controlled group design. The sample used 27 mice, 8-10 weeks old and weights 20 g. Samples were divided into 4 groups. Group of KKand KP1, in order, was only given distilled water and 0.06 ml of reused palm oil in addition to distilled waterfor 14 days. whilegroup of KP2 and KP3 was respectively given soy bean powder at a dose of 5.6 mg and 11.2 mg per 20 g of body weight each of mice alongside with 0.06 ml of reused palm oil and distilled water for 14 days. On 15th day, samples were sacrificed to be made preparations of left and right kidney by methode of parafin block and HE staining. The number of tubule proximal cell was calculated based on quantifying of pyknosis, karyorrhexis, and karyolysis. Data were analyzed using the Kruskal Walis test (? = 0.05) and continued with Mann-Whitney test (? = 0.05). Result: : The Kruskal Wallis test showed that there was significant difference between the four groups. Mann-Whitney test showed there was significant difference between groups of KK-KP1, KK-KP2, KK-KP3, KP1-KP2, KP1-KP3, and KP2-KP3. Conclusion: Soy bean (Glycine max.) can prevent the renal cell damage of mice which are induced by reused palm oil and the increase of soy bean dosage can increase protection effect to renal cell damage of mice which are induced by reused palm oil. Keywords: soy bean, reused palm oil, the renal cell damage

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