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All Journal Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Medical Journal of Indonesia
Jenny Hidayat
Departemen Biokimia - Kimia, Fakultas Kedokteran Unika Atma Jaya
Immunology & microbiology Medicine & Pharmacology Neuroscience

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Plasma lipid pattern and red cell membrane structure in β-thalassemia patients in Jakarta Freisleben, Seruni K.U.; Hidayat, Jenny; Freisleben, Hans-Joachim; Poertadji, Soehardjo; Kurniawan, Budi; Bo, Na P.; Handayani, Sri; Wahidiyat, Pustika A.; Soegianto, Rondang R.
Medical Journal of Indonesia Vol 20, No 3 (2011): August
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (219.552 KB) | DOI: 10.13181/mji.v20i3.448

Abstract

Background: Over the last 10 years, we have investigated thalassemia patients in Jakarta to obtain a comprehensive picture of iron overload, oxidative stress, and cell damage.Methods: In blood samples from 15 transfusion-dependent patients (group T), 5 non-transfused patients (group N) and 10 controls (group C), plasma lipids and lipoproteins, lipid-soluble vitamin E, malondialdehyde (MDA) and thiol status were measured. Isolated eryhtrocyte membranes were investigated with electron paramagnetic resonance (EPR) spectroscopy using doxyl-stearic acid and maleimido-proxyl spin lables. Data were analyzed statistically with ANOVA.Results: Plasma triglycerides were higher and cholesterol levels were lower in thalassemic patients compared to controls. Vitamin E, group C: 21.8 vs T: 6.2 μmol/L) and reactive thiols (C: 144 vs. T: 61 μmol/L) were considerably lower in transfused patients, who exert clear signs of oxidative stress (MDA, C: 1.96 vs T: 9.2 μmol/L) and of tissue cell damage, i.e., high transaminases plasma levels. Non-transfused thalassemia patients have slight signs of oxidative stress, but no significant indication of cell damage. Erythrocyte membrane parameters from EPR spectroscopy differ considerably between all groups. In transfusion-dependent patients the structure of the erythrocyte membrane and the gradients of polarity and fluidity are destroyed in lipid domains; binding capacity of protein thiols in the membrane is lower and immobilized.Conclusion: In tranfusion-dependent thalassemic patients, plasma lipid pattern and oxidative stress are associated with structural damage of isolated erythrocyte membranes as measured by EPR spectroscopy with lipid and proteinthiol spin labels. (Med J Indones 2011; 20:178-84)Keywords: electron paramagnetic resonance spectroscopy, erythrocyte membrane, lipoproteins, oxidative stress, thalassemia, plasma lipids.
Effects of resistant starch of mixed tubers snacks on glucose metabolism, leptin, visceral fat and body mass index in type 2 diabetes mellitus (T2DM) Hidayat, Jenny; Sunarti, .; Mustofa, .; Sadewa, Ahmad Hamim
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 51, No 1 (2019)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (259.686 KB) | DOI: 10.19106/JMedSci005101201906

Abstract

Resistant starch could lower blood glucose, decrease adipocyte in adipose tissue and affect satiety hormones such as leptin. Tubers and pumpkin have high content of resistant starch, but their effectiveness to type 2 diabetes mellitus (T2DM) has not been known clearly. This research was conducted to determine the effectiveness of snack consumption made from tubers and pumpkins to BMI, visceral fat, glucose and leptin levels in the blood of T2DM patients and the correlation between the variables. The research method was pre-post clinical trial. Sixteen T2DM patients were in treatment (RS) and control groups. Subjects in RS group were given snack twice daily for 4 weeks. After following wash out process for 4 weeks, the same subjects was continued as subjects’ control. Paired t-test and/or Wilcoxon-test was used to analyze the differences between values before and after treatment in the group and between groups. Pearson test was used to analyze the correlation of BMI, visceral fat, glucose and leptin level. The visceral fat was increased in RS group (p=0.04) after 4 weeks consuming snack but decrease in control group (p=0.04) without significant change of BMI. Leptin level was decreased (p=0.00) in RS group. Blood glucose significantly decreased (p=0.01) and leptin level increased slightly in control group. Comparing the RS and control group at the end of study, there were significantly different in the variation of visceral fat in the female groups (p=0.05) and leptin (p=0.05). Visceral fat correlated with BMI in the RS and control group. In conclusion, the mixed tubers and pumpkin snack decreased the leptin level but increased visceral fat.
Co-Authors Ahmad Hamim Sadewa Budi Kurniawan HANS-JOACHIM FREISLEBEN Mustofa Mustofa Na P. Bo Pustika A. Wahidiyat Rondang R. Soegianto Seruni K.U. Freisleben Soehardjo Poertadji Sri Handayani Sunarti Sunarti