Yudi Mulyana Hidayat
DIVISI ONKOLOGI-GINEKOLOGI DEPARTEMEN OBSTETRI & GINEKOLOGI RUMAH SAKIT HASAN SADIKIN BANDUNG

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Akt–the Mammalian Target of Rapamycin (mTOR) Pathway Inhibition Increases Cervical Cancer Cell Chemosensitivity to Active Form of Irinotecan (SN-38) Septiani, Leri; Hidayat, Yudi Mulyana; Effendi, Yusuf Sulaeman; Djuwantono, Tono; Luftimas, Dimas Erlangga; Faried, Ahmad
International Journal of Integrated Health Sciences Vol 1, No 1 (2013)
Publisher : International Journal of Integrated Health Sciences

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Abstract

Objective: To investigate the molecular pathway of the cytotoxic effect of SN-38 in human cervical cancer cell lines.Methods: Two human cervical cancer cell lines were treated with various concentrations of irinotecan for 24–72 hours and the sensitivity was analysed using the MTT assay. Apoptosis was further observed through microscopic examinations. The protein expression was determined using Western blot analysis.  Results: CaSki cells demonstrated the highest sensitivity to SN-38, whereas HeLa cells showed the lowest. In cervical cancer cells, SN-38 induced apoptosis through an intrinsic- and extrinsic-pathways. In addition, we showed that SN-38 downregulated the phosphorylation of Akt-mTOR pathways in CaSki cells, but not in HeLa cells. Interestingly, in HeLa cells, which were more suggestive of a resistant phenotype, pre-treatment with LY294002 and rapamycin inhibited activation of Akt-mTOR signaling and significantly enhanced the sensitivity of HeLa cells to SN-38. Conclusions: Irinotecan exerts its anti-neoplastic effects on cervical cancer cells by inducing apoptosis through caspase-cascade. Inhibition of Akt-mTOR, LY294002 and rapamycin, which is targeted to Akt-mTOR pathways, may sensitize irinotecan-resistant cervical cancer cells.Keywords: Akt-mTOR pathways anti-neoplastic drugs, cervix cancer cells, LY294002, rapamycin DOI: 10.15850/ijihs.v1n1.103
Hubungan antara Ekspresi mRNA Gen Epidermal Growth Factor Receptor (EGFR) dengan Penurunan Kadar β-hCG Serum Pasca-evakuasi Mola Hidatidosa Komplit PAULUS, LAURENS DAVID; HIDAYAT, YUDI MULYANA; GANDAMIHARJA, SUPRIADI
Indonesian Journal of Cancer Vol 9, No 3 (2015): Jul - Sept 2015
Publisher : "Dharmais" Cancer Center Hospital

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Abstract

In 2008, the incidence of hydatidiform mole that occurred in West Java around 1:28. Malignant transformation of Complete Hydatidiform Mole (CHM) is influenced by many factors, among others, is the expression of the epidermal growth factor receptor (EGFR). This study uses Historical Cohort to retrieve CHM patients data and trophoblastic tissue paraffin block in 2007-2012, and perform examination of Polymerase chain reaction.Results of the study: 40 CHM patients, 24 with EGFR (-) and 16 with EGFR (+), at 12 weeks monitoring post evacuation using the Mochizuki regression curve was not obtained persistent mole in the group with EGFR (-), while the group of EGFR (+), all showed an increase in β-hCG levels. Relative risk (RR) of 3.4 in the CHM group with EGFR (+) into a persistent mole compared with EGFR (-) at 6th week and RR of 13.0 in CHM group with EGFR (+) into a persistent mole compared with EGFR (-) at 8th week. The conclusion of this study demonstrate the suitability of the hypothesis that the higher mRNA gene expression of EGFR, the slower decrease in serum levels of β-hCG after CHM evacuation and expression EGFR (+) may be a predictor of the occurrence of persistent mole. Pada 2008, insiden mola hidatidosa (MH) yang terjadi di Jawa Barat sekitar 1:28. Transformasi keganasan dari mola hidatidosa komplit (MHK) dipengaruhi oleh banyak faktor, antara lain ekspresi Epidermal Growth Factor Receptor (EGFR). Penelitian ini menggunakan metode historical cohort dengan mengambil data dan parafin block jaringan trofoblas pasien MHK tahun 2007−2012, dan dilakukan pemeriksaan POLYMERASE CHAIN REACTion. Hasil penelitian menunjukkan dari 40 pasien MHK, 24 di antaranya EGFR (-) dan 16 EGFR (+) pada 12 minggu pascaevakuasi menggunakan kurva regresi Mochizuki. Tidak didapatkan mola persisten pada kelompok EGFR (-), sedangkan kelompok EGFR (+) semuanya menunjukkan peningkatan β-hCG (mola persisten). Risiko relatif (RR) sebesar 3,4 pada EGFR (+) menjadi mola persisten dibandingkan dengan EGFR (-) pada minggu ke-6 dan RR sebesar 13,0 pada EGFR (+) menjadi mola persisten dibandingkan dengan EGFR (-) pada minggu ke-8. Hasil penelitian ini menunjukkan kesesuaian dengan hipotesis bahwa semakin tinggi ekspresi EGFR maka semakin lambat penurunan kadar β-hCG serum pasca-evakuasi MHK. Ekspresi EGFR dapat menjadi prediktor terjadinya mola persisten.
Hubungan antara Karakteristik Klinis Pasien Mola Hidatidosa dengan Performa Reproduksi Pascaevakuasi di Rumah Sakit Hasan Sadikin Bandung Harjito, Vanessa Natasha; Hidayat, Yudi Mulyana; Amelia, Indah
Jurnal Sistem Kesehatan Vol 3, No 1 (2017): Volume 3 Nomor 1 September 2017
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (292.166 KB) | DOI: 10.24198/jsk.v3i1.13958

Abstract

Pada penderita mola hidatidosa terdapat beberapa karakteristik klinis yang dapat memengaruhi performa reproduksi, yaitu gambaran histopatologi proliferasi berlebih, besar uterus≥20minggu, kadarβ–hCG>100.000mIU/mL, dan kista lutein praevakuasi.Banyak penderita mola hidatidosa merasa khawatir kondisinya dapat memengaruhi fungsi reproduksinya di kemudian hari.Penelitian ini bertujuan untuk mengetahui hubungan antara karakteristik klinis pasien mola hidatidosa dengan luaran kehamilan pascaevakuasi di Rumah Sakit Hasan Sadikin Bandung. Penelitan analitik komparatif ini menggunakan desain kohort retrospektif. Subjek penelitian adalah penderita mola hidatidosa di Rumah Sakit Hasan Sadikin Bandung pada periode 1 Januari 2010-31 Desember 2015 yang memenuhi kriteria penerimaan penelitian. Penelitian mencari hubungan antara variabel karakteristik klinis risiko tinggi (gambaran histopatologi proliferasi berlebih, besar uterus, kista lutein, dan kadar β-hCG) dengan variabel luaran kehamilan pascaevakuasi. Analisis data menggunakan uji Eksak Fisher dengan interval kepercayaan 95%.Subjek penelitian berjumlah 51orang, terdapat 28orang berkarakteristik klinis risiko tinggi. Hasil penelitian menunjukkan karakteristik klinis dan luaran kehamilan pada tipe mola komplit dan mola parsial tidak memiliki perbedaan signifikan(p>0,05). Tidak terdapat hubungan yang signifikan antara gambaran histopatologi proliferasi berlebih, besar uterus, kista lutein, dan kadar β-hCG dengan luaran kehamilan pascaevakuasi(p>0,05). Penelitian ini menyimpulkan tidak terdapat hubungan antara karakteristik klinis pasien mola hidatidosa dengan performa reproduksi pascaevakuasi di Rumah Sakit Hasan Sadikin Bandung.Kata kunci: karakteristik klinis risiko rendah, karakteristik klinis risiko tinggi, luaran kehamilan, mola hidatidosa
Hubungan Kadar βHCG Praevakuasi, Gambaran Histopatologi, dan Kista Lutein dengan Performa βHCG pada Penderita Mola Hidatidosa yang Berkembang Menjadi PTG dan Kembali Normal Hidayat, Yudi Mulyana; Gandamihardja, Supriadi; Krisnadi, Sofie Rifayani
Majalah Kedokteran Bandung Vol 46, No 4 (2014)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Insidensi penyakit trofoblas di Indonesia maupun negara berkembang masih cukup tinggi dibandingkan dengan negara maju. Hal yang perlu diwaspadai adalah terjadinya penyakit trofoblast gestasional (PTG) pascaevakuasi mola hidatidosa berkisar 10-20%. Beberapa variabel klinis telah diteliti sebagai variabel faktor risiko keganasan seperti kadar β-human chorionic gonadotropin (βHCG) praevakuasi, gambaran histopatologi, dan terdapat kista lutein. Tujuan penelitian ini adalah mengetahui hubungan kadar βHCG praevakuasi, gambaran histopatologi dan kista lutein dengan performa penurunan βHCG pada penderita mola. Metode penelitian yang digunakan adalah case control study pada penderita mola hidatidosa komplet di Rumah Sakit Dr. Hasan Sadikin Bandung selama periode tahun 2007-2011. Hasil penelitian menunjukkan hubungan bermakna kadar βHCG ≥100.000 mIU/mL dengan keganasan pascamola (p<0,05), terdapat hubungan bermakna gambaran histopatologi proliferasi berlebih dengan keganasan pascamola (p<0,05), dan terdapat hubungan bermakna kista lutein positif dengan keganasan pascamola (p<0,05). Simpulan penelitian ini adalah variabel kadar βHCG praevakuasi ≥100.000 mIU/mL, gambaran histopatologi proliferasi berlebih, dan kista lutein positif memiliki korelasi dengan keganasan pascaevakuasi mola. Variabel faktor risiko tersebut  dapat digunakan untuk memilahkan penderita mola hidatidosa komplet risiko tinggi atau risiko rendah untuk kejadian keganasan dan variabel faktor risiko keganasan tersebut berpengaruh pada performa penurunan kurva regresi βHCG.Kata kunci: Gambaran histopatologi, kadar βHCG, kista lutein, mola hidatidosa komplit, PTGRelationship between Pre-Evacuation βHCG Level, Histopathologycal View, Lutein Cysts and βHCG Performance in Patients with Hydatidiform Mole which Developed into Gestational Trophoblastic Disease (GTD) and Back to NormalAbstractThe incidence of trophoblastic diseases in Indonesia and developing countries is relatively high compared to the developed countries. The incidence of gestational trophoblast tumors (GTT) after the evacuation of a hydatidiform mole ranges from 10% to 20%. Several clinical variables have been studied as the risk factors for malignancy, including the pre-evacuation level of beta human chorionic gonadotropin (βHCG), histopathological appearance, and the presence of lutein cysts. The purpose of this study was to determine the relationship between βHCG decline and pre-evacuation βHCG levels, histopathological features, and the lutein cysts status in patients with moles. This study was a case control study of patients with complete hydatidiform mole in Dr. Hasan Sadikin General Hospital during the period of 2007-2011. The results revealed that there was a significant correlation between the level of βHCG ≥100,000 mIU/mL and post-molar malignancy (p<0.05). There was also a significant relationship between the histopathologic feature of excessive post-molar cell proliferation and malignancy (p<0.05) and between the presence of lutein cyst and post-molar malignancy (p<0.05). This study concludes that the pre-evacuation βHCG level ≥100.000 mIU/mL, excessive proliferation, and the presence of lutein cysts are correlated with malignancy after molar evacuation. These risk factors are useful to differentiate whether a complete hydatidiform mole will become malignant or remain benign.Key words: Beta human chorionic gonadotropin levels, histopathologic features, lutein cysts, complete hydatidiform mole, gestational trophoblast tumors DOI: 10.15395/mkb.v46n4.345
Hubungan antara total asupan energi dan komponen makrontrien dengan penambahan berat badan ibu hamil di Kecamatan Pedurungan Kota Semarang Astuti, Yuli; Hidayat, Yudi Mulyana; Rohmawati, Enny
Jurnal Gizi Indonesia (The Indonesian Journal of Nutrition) Vol 9, No 1 (2020)
Publisher : Department of Nutrition Science, Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jgi.9.1.33-41

Abstract

Background: Gestational weight gain (GWG) is physiological change in pregnancy. Inadequate gestational weight gain (IGWG) and excessive gestational weight gain (EGWG) can pose a risk of complication such as gestational hypertention, low birth weight, giant baby, and premature birth. Food intake (energy and macronutrient) play important role in providing pregnancy needs such as fetal and placental growth, and also play role in fat accumulation in some part of mother’s body. Objectives: This study aims to analyze the relationship between energy intake and macronutrient and gestational weight gain of pregnant woman and to know the consumption of food groups.Methods: This study was quantitative study using cohort prospective approach. 73 healthy pregnant women with normal body fat mass who met inclusion and exclusion criteria was weighed twice in a period of 8 weeks. 24 hours food recall in 2 unsecutive days was done between those times. The instruments used for this study were weight scale, stadiometer, and questionnaire. Nutritional analysis used Nutrisurvey and statistic analysis used Chi Square test.Result: There was a relationship between total energy intake and gestational weight gain ( p = 0.031) and there was no relationship between macronutrient component intake (carbohydrate, fat, and protein) and gestational weight gain (p = 0.175, p = 0.97, and p = 0.089 respectively). Respondent characteristic that has corelation with GWG is number of pregnancy. Most pregnant woman experienced EGWG (56.2%) and their food consumption not fulfilled the balance nutrient recommendation guidelines especially less in vegetables and fruits consumption.Conclusion:There is a significant corelation between energy intake and GWG.