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Aplikasi Pupuk Hayati Konsorsium Dan Inokulan Padat Trichoderma harzianum Terhadap Produktivitas Tanaman Sawi Pada Lahan Terkontaminasi Rhizoctonia solani Kalay, A Marthin; Uluputty, M Riadh; Leklioy, Juniart; Hindersah, Reginawanti; Talahaturuson, Abraham
Agrologia Vol 5, No 2 (2016)
Publisher : Pattimura University, Ambon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/a.v5i2.185

Abstract

Choy sum (Brassica rapa L.) cultivation main obstacle in Maluku was soil fertility and plant diseases. Biological agent and Biofertilizers will play a significant role to reduce the intensity of disease attacks, and increase crop productivity. The objective of this experiment  was to study the effects of nitrogen fixing and phosphate solubilizing microbe (Bion-UP) and Trichoderma harzianum solid inokulan (Bokelas Plus) on choy sum productivity grown on land Rhizoctonia solani-contaminated land. The experimental treatments  were liquid  biofertilizer and Bokelas Plus which has been combined into 6 treatments: Without biofertilizer and Bokelas Plus, 50 kg Bokelas Plus/ha without biofertilizer, 15 mL biofertilizer per plant without Bokales Plus, 15 mL biofertilizer per plant + 50 Kg Bokelas Plus/ha, 15 mL biofertilizer  per plant + 25 kg Bokelas Plus/ha, and 7.5 mL biofertilizer per plant + 50 kg Bokales Plus/ha. The results showed that giving of Bokelas Plus and biofertilizer with different dose levels decreased  leaf blight intensity and increasing fresh weight of choy sum. Biofertilizer application with and without Bokelas Plus decreased the intensity of leaf blight disease by 39.14% - 46, 93%, and increase the fresh weight of choy sum by 15.19% - 36.25%. 
Kestabilan Inokulan Azotobacter selama Penyimpanan pada Dua Suhu Hindersah, Reginawanti; Sudirja, Rija
Jurnal Natur Indonesia Vol 14, No 1 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (40.395 KB) | DOI: 10.31258/jnat.14.1.52-55

Abstract

Azotobacter might be used as biological agents in bioremediation of heavy metal-contaminated soil since this rhizobacteria produceexopolysachharides (EPS) that mobilize soil heavy metals, and phytohormones that regulate root growth. So that heavy metal uptake bythe roots could be increased. The objective of this research was to verify the stability of EPS and phytohormones in Azotobacter liquidinoculants during four months in different temperature storage. Liquid inoculants has been produced in EPS-induced media and stored in200C and room temperature (24-270C) during four months. The results showed that the better temperature storage was room temperatureinstead of 20 0C since pH, total N, and EPS and phytohormones content was relatively stable during storage.
Populasi Mikrob di Rizosfer dan Pertumbuhan Caisim (Brassica juncea) di Tanah Dikontaminasi Insektisida Organoklorin setelah Aplikasi Konsorsia Mikrob dan Kompos Hindersah, Reginawanti; Rachman, Wahyuda; Fitriatin, Betty Natalie; Nursyamsi, Dedi
Jurnal Natur Indonesia Vol 15, No 2 (2013)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (79.076 KB) | DOI: 10.31258/jnat.15.2.115-120

Abstract

Organochlorine insecticide hed been banned for agriculture but nowadays its residue was still found in some agriculturalsoil. One of ways to lower organochlorine residue in soil is by using degrading-organochlorine microbial consortiaaccompanied by compost application. However, application of exogenous microbes might affect bacterial and fungalpopulation in soil and hence plant growth. The pot experiment has been set up to verify the influence of Pseudomonasmallei and Trichoderma sp. and compost on total bacteria and fungi on rhizosphere of caisim (Brassica juncea L.) grownon Andisols which were contaminated with organochlorine insecticide of Heptachlor, endosulfan, dieldrin, and DDT.Experimental design was Factorial Randomized Block Design with three replicates. The first factor was microbial consortiaof P.mallei and Trichoderma sp., and the second one was the kind of compost. The result showed that effect of microbialconsortia on total bacteria population was determined by the kind of compost. Total bacterial population in rhizosphere ofcaisim grown with cow manure compost and microbial consortia was more increased. However, microbial consortia as wellas any kind of compost did not influence total fungal population in caisim rhizosphere. Amount of leaf increased due tomicrobial and compost application however plant yield did not.
Pengaruh CdCl2 terhadap Produksi Eksopolisakarida dan Daya Hidup Azotobacter Hindersah, Reginawanti; Arief, Dedeh Hudaya; Soemitro, Soetijoso; Gunarto, Lukman
Jurnal Natur Indonesia Vol 12, No 1 (2009)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (49.62 KB) | DOI: 10.31258/jnat.12.1.34-37

Abstract

The contamination of toxic heavy metal Cadmium (Cd) in soils will be endanger the human health because it ismore available comparing to another toxic heavy metals. One method of Cd-contaminated soil bioremediation isusing exopolysachharide-producing bacteria Azotobacter. Exopolysachharides (EPS) can mobilize Cd through theformation of complex Cd-EPS which sequentially can increase the availability of Cd for plants uptake. A laboratoryexperiment has been done to study the EPS production and the viability of six Azotobacter isolates in the liquidculture containing 0.01, 0.1, and 1 mM CdCl2. The bacteria were cultured in liquid medium with and without CdCl2 for72 hours at room temperature. The EPS production was determined by gravimetric method after precipitationusing acetone and centrifugation at 7000 rpm. The result was that all of Azotobacter isolates produce EPS in thepresence of CdCl2. In the culture with 1 mM CdCl2, the density of Azotobacter sp. isolate BS3, LK5, LKM6 increasedsignificantly, and that of isolate LH16 decreased. No significant effect of CdCl2 on the density of isolate BS2 andLH15. This research suggested that some Azotobacter isolates were relatively resistence to the Cd and could bedeveloped as biological agents in Cd-contaminated soil bioremediation.
Suhu dan Waktu Inkubasi untuk Optimasi Kandungan Eksopolisakarida dan Fitohormon Inokulan Cair Azotobacter sp. LKM6 Hindersah, Reginawanti; Sudirja, Rija
Jurnal Natur Indonesia Vol 13, No 1 (2010)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (59.034 KB) | DOI: 10.31258/jnat.13.1.67-71

Abstract

Azotobacter inoculation could play an important role to enhance the effectiveness of bioremediation since bacterialexopolysachharides form a complex with heavy metal. So that metal mobility in soil and its uptake by plantsincreased. Azotobacter also produce phytohormone which induce roots growth and subsequently the uptake ofnutrients. The objective of this research was to obtain optimal incubation temperature and time in Azotobacter sp.LKM6 liquid inoculants production in the fermentor to maximize the synthesis of exopolysachharides andphytohormones. The experiment arranged in Completely Randomized Design consisted of two incubationtemperature (room temperature and 300C). At 24, 36, and 48 hours incubation, the concentration of EPS andphytohormone cytokinin and giberrelin were occurred. The experimental results were 1) the best temperature andincubation time to produce Azotobacter sp. LKM6 liquid for bioremediation of heavy metal-contaminated soil was30oC and 48 hours, and 2) inoculants production at 300C for 48 jam produce liquid inoculants containing 2.87mg L-1 exopolysachharides, 81.0 mg L-1 cytokinins and 18.7 mg L-1 giberrelin, and 13.12 x 108 cell ml-1.
LIMBAH SAGU: POTENSI LOKAL UNTUK MEDIA PUPUK HAYATI Reginawanti Hindersah; A. Marthin Kalay; Agus Jacob; Abraham Talahaturuson
Jurnal Agroekoteknologi Vol 6, No 1 (2014)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (58.86 KB) | DOI: 10.33512/j.agrtek.v6i1.229

Abstract

Sago starch production leaves solid and liquid wastes that has not been used optimally. Both organic substances still contains a lot of nutrients, and could be processed into raw material for bio-fertilizers media. Solid waste, known as ela sago, has been developed into compost while the liquid waste did not. The objective of this study was to verify volume ratio of liquid inoculant of Azotobacter chroococcum in solid inoculant produced from ela sago; and determine the concentration of sago waste water as a growth medium of biofertilizer A. chroococcum. This study confirms population of A.chroocccum and T. harzianum in ela sago compost reached 106 cfu/g and 108 cfu/g consecutively following enrichment with either 2% or 4% of A. chroococcum liquid inoculant. Sago wastewater can used support A.chroococcum growth. This study proved that waste from sago starch production could be used as a natural medium for biofertilizer.
Effect of Vermicompost and Compound inorganic Fertilizer on Soil Phosphate Availability and Yield of Potatoes (Solanum tuberosum L.) Grown in Andisols Hindersah, Reginawanti; Nabila, Agnia; Yuniarti, Ani
Agrologia Vol 8, No 1 (2019)
Publisher : Pattimura University, Ambon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/a.v8i1.874

Abstract

Potatoes (Solanum tuberosum L.) which are comonly grown  in the highland with Andisols require organic and inorganic fertilizers to maintain soil health  and increase yield. The purpose of field experiment  was to obtain information on the effect of vermicompost with and without NPK fertilizer  on soil acidity,  soil phosphor (P) availability and P uptake in potatoes shoot; as well as yield and quality of tuber. The experimental design was a randomized block design with eight treatments and three replication. The treatment consisted of a combination of vermicompost doses (5 and 10 t/ha)  with NPK fertilizer doses (0; 0.5 t/ha and 1 t/ha). The two control treatments were 1) without fertilizer and 2) the method of fertilizing local farmers included 10 t /ha chicken manure and 1 t/ha NPK fertilizer. This experiment verified  that vermicompost and NPK fertilizer increased plant height as well as soil P availability and acidity compared to those of control. Vermicompost has not yet substitute chicken manure to obtain the same tuber production although the percentage of marketable tuber was quite similar.  Keywords : Andisols, NPK Fertilizer, Phosphorus, Potato, Vermicompost.
Azotobacter chroococcum Dan Pembenah Tanah Untuk Menurunkan Serapan Kadmium Oleh Tanaman Padi (Oryza sativa L.) Hindersah, Reginawanti; Nurfitriana, Nofalia; Fitriatin, Betty Natalie
Agrologia Vol 6, No 1 (2017)
Publisher : Pattimura University, Ambon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/a.v6i1.176

Abstract

Heavy metal contamination in paddy field which came from industry nearby and agricultural input. Increased level of toxic heavy metal cadmium will threat rice production and quality. Bioremediation by used of rhizosphere bacteria is an easy, cheap and effective method to control toxic metal uptake. Pot experiment has been done to study the change in cadmium uptake after biofertilizer Azotobacter chroococcum inoculation and some soil ameliorant. Expirement has been carried out in factorial completely randomized block design with A. chroococcum and ameliorant as treatments.   Either Azotobacter inoculation or soil ameliorant didn’t change soil acidity, the soil still neutral. Rhizosphere of paddy received biochar, hay compost and biochar and inoculated with 108 cfu mL-1 Azotobacter was colonized by more Azotobacter compared to uninoculated one. All plant treated with Azotobacter has higher nitrogen uptake but lower cadmium uptake. This experiment suggested that biofertilizer Azotobacter has a significant role to decrease cadmium uptake by padi, an important food crop. 
Populasi Bakteri Pelarut Fosfat, P-Potensial Dan Hasil Jagung Yang Dipengaruhi Oleh Aplikasi MPF Pada Ultisols Jatinangor Fitriatin, Betty Natalie; Agustina, Mayang; Hindersah, Reginawanti
Agrologia Vol 6, No 2 (2017)
Publisher : Pattimura University, Ambon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/a.v6i2.171

Abstract

Ultisol is low fertility soil and as low available P due to P fixation by Al and Fe. The phosphate solubilizing microbes (PSM) is the microbial group that enable realease fixed-P to soil solution and be absorbed by plant. This research was aimed to determine the effects of PSM biofertilizer and phosphate fertilizer dosage combination on soil potential P, total phosphate solubilizing bacteria (PSB) population and yield of maize (Zea mays L.) grown in Ultisols. This research used a Randomized Block Design that consist of nine treatments with three replications. The treatments were 100 kg ha-1 of SP-36 fertilizer, 50 kg ha-1 of PSM biofertilizer, SP-36 fertilizer (50 kg ha-1 and 100 kg ha-1) with PSM biofertilizer (25 kg ha-1, 50 kg ha-1 and 75 kg ha-1) repectively. The result showed that the combination of PSM and phosphate fertilizer dosage had significantly effect on PSB total population and maize yield, but did not not on  potential P. This experiment suggested that SP-36 fertilizer with 50 kg ha-1 dosage and PSM biofertilizer with 50 kg ha-1 dosage is the best combination.
Respons Pertumbuhan Vegetatif Jagung Di Tailing Tambang Timah Terkontaminasi Kadmium Setelah Inokulasi Bakteri Indogenus Reginawanti Hindersah; James Matheus
Agrologia Vol 4, No 1 (2015)
Publisher : Faculty of Agriculture, Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/a.v4i1.211

Abstract

Contaminant levels of cadmium (Cd) in tin mine tailings often exceeds the upper limit.  Relatively high Cd content might  decrease crop yields and threatened food chain. Bioremediation by using  indigenous microbes to lower soil Cd shoud be done prior to use tailings for cultivation. The objective of this experiment was to obtain information about the capacity of  indigenous bacterias to induce bacterial proliferation  in the rhizosphere, corn growth during the early vegetative stage and change the concentration of Cd in soil and plants. Pot experiment was set up in  a Randomized Block Design consist of several combination of  two bacterial isolates and their doses.  Maize seeds were sown in  tailings from the mine site on the island of Bangka and inoculated with indigenous bacteria. The results showed that the bacterial inoculation increased number of bacteria in the rhizosphere  and plant height, but only isolates BKH1 with a dose of 5 mL/plants that increased plant dry weight. Soil Cd content decreased when inoculated by bacteria although not statistically significant, while the effect of inoculation on plant Cd uptake was inconsistent.