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Penggunaan Demonstrasi plot untuk Mengubah Metode Aplikasi Pupuk Organik pada Lahan Pertanian Sayuran di Kota Ambon Hindersah, Reginawanti
Dharmakarya Vol 5, No 1 (2016): Dharmakarya
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (349.542 KB)

Abstract

Sayuran daun  adalah  pangan penting dalam  makanan sehari-hari masyarakat Kotamadya Ambon Maluku. Di musim hujan, sayuran menjadi penyebab inflasi. Ambon juga mengimpor sayuran  non daun dari beberapa kota di Jawa dan Sulawesi.  Salah satu  masalah produktivitas sayuran adalan supply pupuk anorganik yang tidak kontinyu dan harganya mahal.  Pemupukan organik dapat menjadi alternatif, saat ini petani sudah  menggunakan pupuk organik berupa kotoran ayam dengan cara ditaburkan. Metode ini diyakini tidak efisien dan kurang efekfif. Tujuan pertama  dari Program Pengabdian kepada Masyarakat ini bertujuan untuk memperoleh informasi mengenai pemahaman petani terhadap fungsi pupuk.  Tujuan kedua adalah melaksanakan demonstrasi plot cara aplikasi pupuk organik yang benar di lahan pertanian  sayuran daun, dalam kerangka peningkatan produktivitasnya. Kegiatan Focus Group Discussion sebelum demonstrasi plot dilakukan di area pertanian sayuran Dusun Waiheru Desa Waiheru Kecamatan Baguala Kota Ambon untuk satu musim tanam di musim kemarau.  Dua perlakuan demonstrasi plot adalah aplikasi pupuk kotoran  di permukaan tanah setelah tanaman tumbuh; dan pupuk kotoran dicampur dengan tanah bagian atas sebelum tanam. FGD memberikan kejelasan bahwa petani cukup mengerti peran pupuk organik namun ketiadaan pupuk organik kadang-kadang menghambat aplikasi yang tepat waktu. Sebagian besar petani juga tidak berminat membuat pupuk organik sendiri. Demplot di pertanaman sawi hijau (Brassica rapa L.), kangkung (Ipomoea aquatica L.) dan bayam (Amaranthus sp.) memperlihatkan bahwa pencampuran bahan organik dengan tanah dapat  meningkatkan hasil sayuran dibandingkan dengan penaburan bahan organik di permukaan tanah.Kata kunci: Ambon, Demonstrasi Plot, Pupuk Organik, Sayuran Daun.
Azotobacter chroococcum Dan Pembenah Tanah Untuk Menurunkan Serapan Kadmium Oleh Tanaman Padi (Oryza sativa L.) Hindersah, Reginawanti; Nurfitriana, Nofalia; Fitriatin, Betty Natalie
Agrologia Vol 6, No 1 (2017)
Publisher : Pattimura University, Ambon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/a.v6i1.176

Abstract

Heavy metal contamination in paddy field which came from industry nearby and agricultural input. Increased level of toxic heavy metal cadmium will threat rice production and quality. Bioremediation by used of rhizosphere bacteria is an easy, cheap and effective method to control toxic metal uptake. Pot experiment has been done to study the change in cadmium uptake after biofertilizer Azotobacter chroococcum inoculation and some soil ameliorant. Expirement has been carried out in factorial completely randomized block design with A. chroococcum and ameliorant as treatments.   Either Azotobacter inoculation or soil ameliorant didn’t change soil acidity, the soil still neutral. Rhizosphere of paddy received biochar, hay compost and biochar and inoculated with 108 cfu mL-1 Azotobacter was colonized by more Azotobacter compared to uninoculated one. All plant treated with Azotobacter has higher nitrogen uptake but lower cadmium uptake. This experiment suggested that biofertilizer Azotobacter has a significant role to decrease cadmium uptake by padi, an important food crop. 
Suhu dan Waktu Inkubasi untuk Optimasi Kandungan Eksopolisakarida dan Fitohormon Inokulan Cair Azotobacter sp. LKM6 Hindersah, Reginawanti; Sudirja, Rija
Jurnal Natur Indonesia Vol 13, No 1 (2010)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (59.034 KB) | DOI: 10.31258/jnat.13.1.67-71

Abstract

Azotobacter inoculation could play an important role to enhance the effectiveness of bioremediation since bacterialexopolysachharides form a complex with heavy metal. So that metal mobility in soil and its uptake by plantsincreased. Azotobacter also produce phytohormone which induce roots growth and subsequently the uptake ofnutrients. The objective of this research was to obtain optimal incubation temperature and time in Azotobacter sp.LKM6 liquid inoculants production in the fermentor to maximize the synthesis of exopolysachharides andphytohormones. The experiment arranged in Completely Randomized Design consisted of two incubationtemperature (room temperature and 300C). At 24, 36, and 48 hours incubation, the concentration of EPS andphytohormone cytokinin and giberrelin were occurred. The experimental results were 1) the best temperature andincubation time to produce Azotobacter sp. LKM6 liquid for bioremediation of heavy metal-contaminated soil was30oC and 48 hours, and 2) inoculants production at 300C for 48 jam produce liquid inoculants containing 2.87mg L-1 exopolysachharides, 81.0 mg L-1 cytokinins and 18.7 mg L-1 giberrelin, and 13.12 x 108 cell ml-1.
Pengaruh CdCl2 terhadap Produksi Eksopolisakarida dan Daya Hidup Azotobacter Hindersah, Reginawanti; Arief, Dedeh Hudaya; Soemitro, Soetijoso; Gunarto, Lukman
Jurnal Natur Indonesia Vol 12, No 1 (2009)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (49.62 KB) | DOI: 10.31258/jnat.12.1.34-37

Abstract

The contamination of toxic heavy metal Cadmium (Cd) in soils will be endanger the human health because it ismore available comparing to another toxic heavy metals. One method of Cd-contaminated soil bioremediation isusing exopolysachharide-producing bacteria Azotobacter. Exopolysachharides (EPS) can mobilize Cd through theformation of complex Cd-EPS which sequentially can increase the availability of Cd for plants uptake. A laboratoryexperiment has been done to study the EPS production and the viability of six Azotobacter isolates in the liquidculture containing 0.01, 0.1, and 1 mM CdCl2. The bacteria were cultured in liquid medium with and without CdCl2 for72 hours at room temperature. The EPS production was determined by gravimetric method after precipitationusing acetone and centrifugation at 7000 rpm. The result was that all of Azotobacter isolates produce EPS in thepresence of CdCl2. In the culture with 1 mM CdCl2, the density of Azotobacter sp. isolate BS3, LK5, LKM6 increasedsignificantly, and that of isolate LH16 decreased. No significant effect of CdCl2 on the density of isolate BS2 andLH15. This research suggested that some Azotobacter isolates were relatively resistence to the Cd and could bedeveloped as biological agents in Cd-contaminated soil bioremediation.
Populasi Mikrob di Rizosfer dan Pertumbuhan Caisim (Brassica juncea) di Tanah Dikontaminasi Insektisida Organoklorin setelah Aplikasi Konsorsia Mikrob dan Kompos Hindersah, Reginawanti; Rachman, Wahyuda; Fitriatin, Betty Natalie; Nursyamsi, Dedi
Jurnal Natur Indonesia Vol 15, No 2 (2013)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (79.076 KB) | DOI: 10.31258/jnat.15.2.115-120

Abstract

Organochlorine insecticide hed been banned for agriculture but nowadays its residue was still found in some agriculturalsoil. One of ways to lower organochlorine residue in soil is by using degrading-organochlorine microbial consortiaaccompanied by compost application. However, application of exogenous microbes might affect bacterial and fungalpopulation in soil and hence plant growth. The pot experiment has been set up to verify the influence of Pseudomonasmallei and Trichoderma sp. and compost on total bacteria and fungi on rhizosphere of caisim (Brassica juncea L.) grownon Andisols which were contaminated with organochlorine insecticide of Heptachlor, endosulfan, dieldrin, and DDT.Experimental design was Factorial Randomized Block Design with three replicates. The first factor was microbial consortiaof P.mallei and Trichoderma sp., and the second one was the kind of compost. The result showed that effect of microbialconsortia on total bacteria population was determined by the kind of compost. Total bacterial population in rhizosphere ofcaisim grown with cow manure compost and microbial consortia was more increased. However, microbial consortia as wellas any kind of compost did not influence total fungal population in caisim rhizosphere. Amount of leaf increased due tomicrobial and compost application however plant yield did not.
Kestabilan Inokulan Azotobacter selama Penyimpanan pada Dua Suhu Hindersah, Reginawanti; Sudirja, Rija
Jurnal Natur Indonesia Vol 14, No 1 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (40.395 KB) | DOI: 10.31258/jnat.14.1.52-55

Abstract

Azotobacter might be used as biological agents in bioremediation of heavy metal-contaminated soil since this rhizobacteria produceexopolysachharides (EPS) that mobilize soil heavy metals, and phytohormones that regulate root growth. So that heavy metal uptake bythe roots could be increased. The objective of this research was to verify the stability of EPS and phytohormones in Azotobacter liquidinoculants during four months in different temperature storage. Liquid inoculants has been produced in EPS-induced media and stored in200C and room temperature (24-270C) during four months. The results showed that the better temperature storage was room temperatureinstead of 20 0C since pH, total N, and EPS and phytohormones content was relatively stable during storage.
FUNGI MIKORIZA ARBUSKULA ASAL PANGALENGAN JAWA BARAT SEBAGAI AGENS HAYATI PENGENDALI NEMATODA SISTA KENTANG Nurbaity, Anne; Sunarto, Toto; Hindersah, Reginawanti; Solihin, Amir; Kalay, Marthin
JURNAL AGROTROPIKA Vol 16, No 2 (2011): Agrotropika Vol.16 No.2 2011
Publisher : JURNAL AGROTROPIKA

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Abstract

Potato cyst nematode (PCN) is known as organism that could suppress the growth of potatoes and it has been found in West Java. Controlling of PCN by the use of chemical agents has been reduced, hence the use of biological agents such as soil fungi is one of the alternatives. Arbuscular mycorrhizal fungi (AMF) has a potential to be used as a controlling agents. However, the information about it?s effect on nematode, especially in Indonesia is limited. The objectives of this study were to isolate the indigenous AMF from potatoes plantation in a high land at Pangalengan district, West Java, to be used as an agents for controlling nematodes. AMF isolates were cultured and propagated to be used in the test of AMF effectiveness in controlling PCN in a glasshouse. Results showed that AMF at the rate of 150 spores/pot reduced the PCN parameters in term of numbers of juveniles II, fe-males and cysts. The mechanisms of AMF in reducing PCN activities was more likely because of the pro-duction of antifungi isoflavonoid that increased as increasing the rate of AMF spores. The experiment in general showed the successful results in explaining the potential use of AMF as bio-control agents of PCN.  Keywords: Arbuscular Mycorrhizal Fungi, Potato Cyst Nematode
Peningkatan Populasi, Pertumbuhan dan Serapan Nitrogen Tanaman Kedelai dengan Pemberian Azotobacter Penghasil Eksopolisakarida Reginawanti Hindersah; Neni Rostini; Arief Harsono; dan Nuryani
Indonesian Journal of Agronomy Vol. 45 No. 1 (2017): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (346.151 KB) | DOI: 10.24831/jai.v45i1.13801

Abstract

Nitrogen-fixing Azotobacter is widely used as biofertilizer in sustainable agriculture. The bacteria produce exopolysaccharide which might have a significant role in enhancing soybean nitrogen uptake and growth. The objective of this research was to obtain growth media of Exopolysaccharide–producing Azotobacter; and increase shoot and root growth as well as nitrogen uptake of soybean var. Anjasmoro at early vegetative phase following inoculation of Azotobacter chroococcum liquid. Research consist of two phase, 1) determination of organic-based media for A. chroococcum liquid inoculant production, and 2) pot experiment for application of liquid inoculant on soybean.  The first experiment was performed  in a series of  batch fermenter consisted of several organic media for 72 hours.  The second experiment was set in completely randomized design consisted of three density of liquid inoculant. The results verified that the best media which induced exopolysachharide production of A. chroococcum was 1% molase enriched with 0.1% NH4Cl. Liquid inoculant clearly enhanced population of Azotobacter in soybean rhizosphere, plant height, roots dry weight and N uptake of 21 day old soybean. This research implied that A. chroococcum might be used as biofertilizer at early growth of soybean. Keywords: Azotobacter chroococcum, biofertilizer, liquid inoculat
Peran Eksopolisakarida Azotobacter dan Bahan Organik untuk Meningkatkan Nodulasi dan Biomassa Kedelai pada Dua Ordo Tanah Reginawanti Hindersah; Neni Rostini; Agustinus Marthin Kalay; dan Arief Harsono
Indonesian Journal of Agronomy Vol. 47 No. 2 (2019): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (357.77 KB) | DOI: 10.24831/jai.v47i2.23328

Abstract

Exopolysaccharide (EPS) produced by nitrogen-fixing bacteria Azotobacter protect nitrogenase from oxygen. In legume,EPS plays a role in the immobilization of rhizobia to the roots. The objective of this experiment was to study the effect of EPSAzotobacter and organic matter on increasing number of nodules and biomass of soybeans grown in Inceptisols and Ultisols;as well as nitrogen-fixing bacteria population in soybean rhizosphere. The experiment was set up in a completely randomizedblock design with five replications to test combined treatments of two doses of crude EPS and organic matter. Nodule number,shoot dry weight and nitrogen uptake, as well as Azotobacter and Rhizobium population in soybean grown in Inceptisolsfollowing crude EPS and compost application, were higher than those grown in Ultisols. The application of EPS and compostIn Ultisols did not affect the number of nodule and other traits, but in Inceptisols, adding 6.25 g of compost and 20 mL of EPSto each plant increased the number of nodules and shoot weight at 42 days after planting. However, the highest N uptake wasdemonstrated by soybean received 10 mL and 20 mL EPS along with 12.5 g compost.
Pengaruh Topsoil dan Pupuk Organik Terhadap Panjang Sulur dan Jumlah Daun Tanaman Ubi Jalar (Ipomoea Batatas Lamb.) pada Media Tailing Emas: The Effect of Topsoil and Organic Manure Towards Length of Vein and Number of Laeves of Sweet Potatoes (Ipomoea Batatas Lamb.) in Gold Tailing Ninda Meiditia Putri; Rhazista Noviardi; Reginawanti Hindersah; Pujawati Suryatmana
Jurnal Ilmu Tanah dan Lingkungan Vol 23 No 1 (2021): Jurnal Ilmu Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jitl.23.1.33-37

Abstract

Processing of gold ore at artisanal gold mining in Kertajaya Village, Sukabumi, West Java produces residual processing waste or tailings which are generally disposed of into rivers or farm, stored in storage ponds or put in sacks for reprocessing. Due to the limited land owned by the community, the ponds and farm that were formerly dumped for tailings are widely used for farming. This study aims to determine the effect of adding topsoil and compost dose on the growth of sweet potato (Ipomoea batatas) at the maximum vegetative phase (7 Weeks after planted). The topsoil used in this study was taken from Kertajaya Village, which is an inceptisol soil type, while the sweet potato plant used was sweet potato clone MZ119. The research was conducted at the Ciparanje Experimental Farm, Faculty of Agriculture UNPAD, Sumedang from January to May 2020. This experiment used a factorial randomized block design with two factors, namely the topsoil-tailing ratio of 3 levels (30% w/w; 50% w/w; and 70% w/w) and compost doses of 4 levels (without compost (control); 10 tonnes ha-1; 20 tonnes ha-1; and 30 tonnes ha-1) with 3 replications. The results showed that the combination treatment of 70% topsoil: 30% tailings and compost dosage of 30 tonnes ha-1 could increase the length of sweet potato vein in the maximum vegetative phase which was 24.33 cm and the number of leaves was 54.33.
Co-Authors A Marthin Kalay A Marthin Kalay A. Marthin Kalay A. Marthin Kalay A. Marthin Kalay Abraham Talahaturuson Abraham Talahaturuson Ade Setiawan Adita Rizky Syntianis Agnia Nabila Agung Karuniawan Agus Jacob Agustinus Marthin Kalay Ai Siti Santriyani Alia Halimatusy Aliya Zahrah Adawiah Alyani Shabrina Amiruddin Teapon Ane Nurbaity Ani Yuniarti Anne Nurbaity ANNE NURBAITY Anni Yuniarti Anny Yuniarti Antonio Yusuf Arief Harsono Asep Purnama Hidayat Aten Komarya Bagus Adityo Bari, Ichsan Nurul Benito R. Kurnani Benny Joy Betty N. Fitriatin Betty Natalie Fitriatin Betty Natalie Fitriatin Betty Natalie Fitriatin Camilla Salsabilla D Herdiyantoro D. A Sulaksana Dadang Epi Sukarsa dan Arief Harsono dan Nuryani Dedeh H. Arief Dedeh Hudaya Arief Dedi Nursyamsi Dedi Nursyamsi Dedi Nursyamsi Dedi Nursyamsi Dedi Nursyamsi Dedy Nursyamsi Dewikusuma Ikhsani Diana Nafitri Cahayaningrum Diky Indra Wibawa Dirga Sapta Sara Diyah Sri Utami DIYAN HERDIYANTORO Diyan Herdiyantoro Diyan Herdiyantoro Dwi Suci Rahayu ELENA F. L. LILIPALY Erni Suminar Etty Pratiwi Fasa Aditya Fauzul Amri Febby Nur Indriani Febby Nur Indriani Fera Siti Meilani Ferra Langoi Fiky Yulianto Wicaksono Gina Nurhabibah Gita Bina Nugraha H Yulina Haris Maulana Harry Rum Haryadi Henry Kesaulya Herman Rehatta Hidiyah Ayu Ratna Ma’rufah Ibnu Haikal Iis Nur Asyiah Imam Mudakir Imran Makatita Indra Herliana Irene A. Ngabalin Ishak, Lily James Matheus June A Putinella June Putinella Juniart Leklioy Lukman Gunarto M Riadh Uluputty Mahdi Argawan Putra Mahfud Arifin Mardiyani Sidayat Marina Jamlean Martha Fani Cahyandito Marthin Kalay Marthin Kalay Maya Damayani Mayang Agustina Meddy Rachmadi Mieke Rochimi Setiawati Mieke Rochimi Setiawati Mieke Rochimi Setiawati Mieke Rochimi Setiawati Mieke Rochimi Setiawati Mieke Rochimi Setiawati Mieke Rochimi Setiawati Mudiyati Rahmatunnisa Mulyadi Mulyadi Nadia Nuraniya Kamaluddin Nadia Nuraniya Kamaluddin Nadia Rachelita Nandha Afrilandha NANJAPPA ASHWATH Neni Rostini Neni Rostini Nenny Nurlaeny NENNY NURLAENY Nia Kurniati Nia Kurniati Nia Kurniati Ninda Meiditia Putri Nini Mila Rahmi Nizar Ulfah Nono Carsono Nurfitriana, Nofalia Osok, Rafael M Oviyanti Mulyani Priyanka Asmiran Priyanka Asmiran Priyanka Asmiran PRIYANKA PRIYANKA Probo Condrosari Pudjawati Suryatmana Pujawati Suryatmana Puji Syara Anggia Putri Sri Judiani Purba Rachmat Harryanto Rara Rahmantika Risanti Rara Rahmatika Risanti Rhazista Noviardi Rhazista Noviardi Riadh Uluputty Rija Sudirja Rija Sudirja Rina Devnita Rita Harni Rita Harni Robby Risamasu Robi Risamasu Rustam Rustam Sandra Amalia Riyadi Saon Banerjee Sarita Sarkar Shabrina Rahma Fauzia Soetijoso Soemitro Solihin, Amir Sondi Kuswaryan Suman Samanta Sunardi Sunardi Tarkus Suganda Toto Sunarto Tri Hanggono Achmad Triani Dewi Triani Dewi Triyani Dewi Triyani Dewi Triyani Dewi Tualar Simarmata Vera Oktavia Subarja Vira Kusuma Dewi Wahyuda Rachman, Wahyuda Wawan Kurniawan Wawan Rachman Wilhelmina Rumahlewang Wilhelmina Rumahlewang Wilhemina Rumahlewang Yansen Lakburlawal Yuliati Mahfud Yusi Firmansyah Yusuf Rahman Yusup Hidayat Zellya Handyman