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Performance, carcass precentages and heterosis values, Alabio and Cihateup line and crossbreeding on eight week old P.R. Matitaputty; R.R. Noor; P.S. Hardjosworo; C.H. Wijaya
Jurnal Ilmu Ternak dan Veteriner Vol 16, No 2 (2011): JUNE 2011
Publisher : Indonesian Center for Animal Research and Development (ICARD)

One effort for improving the productivity of Indonesian local ducks in particular as a meat types is by crossing among the local ducks. Crossbreeding is a fast breeding program for improving some commercial traits. Crossing between two lines or different breed are often used in production systems to utilized the advantage of heterosis (hybrid vigor). Crossbreeding is expected to produce new synthetic strains of duck that perform better than their parents. This study aims to utilize the heterosis and determine best cross, and to study the performance changes due to crossbreeding. The statistical design used in this study was a randomized complete design (RCD) consisting of 4 treatments with 6 replications, each replication consisting of five ducks. The treatments used were the crossing of the pure Cihateup ♂ x Cihateup ♀ (CC), line Alabio ♂ x Alabio ♀ (AA), Alabio ♂ x Cihateup ♀ (AC) cross as well as Cihateup ♂ x Alabio ♀ (CA) cross. This study using male ducklings first hybrid (F1). The results show that the CA is better than AC in the final live weight (7.05%), weight gain (7.32%), carcass weight (9.24%) and carcass yield (2.55%). Fleshy carcass cuts such as breast meat of AA (6.13%) is better when compared to the others, but the highest percentage of meat percentage of the thigh is the AC (10.13%). Duck crossesing CA possess superior properties more and economic value compared with the duck AC. Key Words: Alabio Duck, Cihateup Duck, Crossing, Heterosis, Performance

Molecular characterization of six sub population Indonesian local goats based on mitochondrial DNA D-loop Aron Batubara; R.R. Noor; A. Farajallah; B. Tiesnamurti; M. Doloksaribu
Jurnal Ilmu Ternak dan Veteriner Vol 16, No 1 (2011): MARCH 2011
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Indonesian local goats were spread in some region, but there was still limited data’s known about the characteristics of its genetic diversity and origin. The Mitochondrial DNA D-loop sequences were used to study the genetic diversity and relationships of six sub population Indonesian local goats, namely, Kacang, Marica, Samosir, Jawarandu, Muara and Bengali goats. From 539 blood samples and DNA extraction collections were selected about 60 samples (10 samples each sub populations) analyzed by PCR-RFLP methods, followed sequence analyzed about 5 PCR products each sub population. The results of the sequence analyses were edited and acquired about 957 bp of nucleotides length. After the alignment analyses were found 50 polymorphic sites which divided into 19 haplotype groups of mtDNA D-loop region. The value of nucleotide diversity was 0.014 ± 0.002. Analysis of Neighbour Joining with Kimura 2 Parameter methods and bootstrap test with 1000 replication indicated that each sub population groups was significantly different between one groups to the others. The maternal lineages origin of six breeds of Indonesian local goats was included to the group of lineage B. The Lineage B was the maternal origin of the haplogroup of goats in the region of East Asia, South Asia, China, Mongolia, North and South Africa, Malaysia, Indonesia, Pakistan and India. Key words: Genetic Diversity, mtDNA D-loop, Haplotypes, Local Goats

The qualitative variation of Katingan cattle Bambang Ngaji Utomo; R.R. Noor; C Sumantri; I Supriatna; E.D Gunardi; B Tiesnamurti
Jurnal Ilmu Ternak dan Veteriner Vol 17, No 1 (2012): MARCH 2012
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The characterization is main activities in order to identify important trait of Katingan cattle. Information of its phenotypic characteristic is not available yet. The aim of the study is to describe the qualitative characteristic of Katingan cattle. Three location of the study were sub-district of Tewah Sanggalang Garing (Pendahara Village), sub-district of Pulau Malan (Buntut Bali Village), and sub-district of Katingan Tengah (Tumbang Lahang Village). The samples of Katingan cattle were taken as many as possible based on the field conditions to observe pattern of colour, growth of horn and gibbosity appear among two horns. General characteristic of Katingan cattle was various in colours of coat, having horns, humped and dewlop. The main characteristics were expressed at female of Katingan cattle. There were six variations of horn shape at female, however the growth of arching forwards was dominant (78.4%). Horn shape of male was generally grown upside (98.3%). Gibbosity was founded among horn only at female cattle (92.6%). There were 9 combinations of coat colour of female Katingan Cattle, those were brown reddish (27%), brown whitish (14.1%), brown like colour of Bali cattle (13.8%), black (12.5%), brown dull (9.6%), brown sorrel (9.3%), blackish (7.1%), white brownish (5.5%) and white grayish (4.5%). Male of Katingan cattle had eight colour combinations, those were brown whitish (14.8%), brown whitish and reddish (14.8%), brown reddish (13.1%), blackish (12.3%), brown whiteish with black withers (10.7%), brown sorrel (9.8%) and brown sorrel with black withers (7.8%). Based on qualitative analysis, the Katingan cattle had variations in coat of colour, growth of horn and the gibbosity. The variation of coat colour had potentially selection related to the value of cultural. Key Words: Katingan Cattle, Qualitative, Coat Colour, Horn, Gibbosity

Moleculer analysis of genotype kappa casein and composition of goat milk Etawah grade, Saanen and their crossbred Yayu Zurriyati; R.R. Noor; R.R.A. Maheswari
Jurnal Ilmu Ternak dan Veteriner Vol 16, No 1 (2011): MARCH 2011
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Polymorphism of goat casein gene closely linked to the quality of milk protein. κ-casein is one of the casein fractions that influence the shape and stability of grain milk. This study is aimed to identify the variation of genotype κ-casein and related with milk quality from Etawah grade, Saanen and their crossbreed (PE-SA). The number of dairy goats used in this study was 150 animals consisted of Etawah grade (48 animals), Saanen (51 animals) and PE-SA (51 animals). Steps of experiment were: blood and milk sampling collection, DNA amplification by PCR and the product digestion using Pst1 enzyme, κ-casein gene sequencing and analyzing the quality of fresh milk. The results showed that κ-casein gene is monomorphic by PCR-RFLP (Pst1) for all the goat breeds, but DNA sequencing indicated 38 point of mutation. Observation on goat milk quality showed that Etawah grade milk had highest (P < 0.05) density value (1.033 ± 0.002) and solid non fat (9.577 ± 0.704%) than those of Saanen and PE-SA fresh milk goat. Key Words: Dairy Goats, Κ-Casein Gene, PCR-RFLP, Milk

Evaluation of genetic diversity of Katingan catlle and their genetic relationship with some other local catlle through DNA microsatellite analysis Bambang Ngaji Utomo; R.R. Noor; c. Sumantri; I Supriatna; E.D. Gurnadi
Jurnal Ilmu Ternak dan Veteriner Vol 16, No 2 (2011): JUNE 2011
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Katingan cattle is one of Indonesian animal genetic resources located in Katingan District, Central Kalimantan. The Katingan cattle is predicted to be extint, therefore it is necessary to conserve the cattle. Breed characterization is the primary step in any conservation program. Characterization was done using 10 microsatellite markers to evaluate the genetic diversity of Katingan Cattle in three subpopulations and their genetic relationship with some other local cattle. A total number of 72 random whole blood of Katingan samples consisted of Pendahara (20 samples), Buntut Bali (20 samples), and Tumbang Lahang (32 samples) were used. The samples of Bali, PO, and Limousine Cattle were 11, 6, and 3 respectivelly. The number of 136 aleles were found with the variation from 9 aleles (ILSTS089) to 18 aleles (ILSTS013) and the average of the alele was 13.6 aleles per locus. A number of alel from Tumbang Lahang was higher (10.8 aleles) than Pendahara (10.4 aleles) and Buntut Bali subpopulation (7.3 aleles). Some loci produced polymorphic diagnostic aleles which varied from 1-7 types of allele. HEL013 and BM1818 had four aleles, while ILSTS026 and ILSTS089 had five and six aleles respectively. ILSTS029 and ILSTS036 had seven aleles. The diagnostic aleles were also found in Tumbang Lahang subpopulastion, as well as in Pendahara, and Buntut Bali. Heterozigositas values of Pendahara, Buntut Bali, and Tumbang Lahang subpopulation were 0.454, 0.478, and 0.529 respectively. While the average of heterozigositas (Ĥ) was 0.492. Subpopulation of Tumbang Lahang was closer genetically to Pendahara (0.169) than Buntut Bali (0.173) and also the subpopulation was closer genetically to PO cattle (0.259) when compared to Buntut Bali (0.311) and Pendahara (0.329). The population of Katingan cattle was within one kluster with PO Cattle. Key Words: Katingan Cattle, Microsatellite, Genetic Diversity

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