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INDUKSI KALUS DAN ORGANOGENESIS KULTUR IN VITRO Dendrobium lineale Rolfe Hoesen, Djadja Siti Hazar; Witjaksono, Witjaksono; Sukamto, LA
BERITA BIOLOGI Vol 9, No 3 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i3.790

Callus induction and organogenesis were evaluated in the protocorm like bodies (PLB) culture of Dendrobium lineale Rolfe (Orchidaceae). The globular PLB were cultured in Murashige and Skoog (MS) medium with half strength macronutrients QA MS), supplemented with thidiazuron (TDZ 0,1, 0,5 and 1) mg/1 and 2,4 Dichlorophenoxy acetic acid (2,4-D 0.1, 0.5, 1 and 5) mg/1 as treatments. The media containing 1 mg/1 TDZ and (1 and 5) mg/1 2,4-D was the best treatment for callus initiation (100%). The largest diameter of callus was obtained from 1 mg/1 TDZ (average 3.5 cm). Shoot buds regeneration achieved on 1 mg/1 TDZ (average 41.66 %). However roots regeneration was very low (average 6.66 %) obtained from 1 mg/1 2,4-D. The number of adventitious buds produced from the regenerated shoots on media without plant growth regulator

PERBANYAKAN ULIN (Eusideroxylon zwageri T.et.B) DENGAN BUI DAN SETEK Utami, Ning Wikan; Hoesen, Djadja Siti Hazar; Witjaksono, Witjaksono; Danu, Danu
BERITA BIOLOGI Vol 7, No 4 (2005)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The ulin or so called vernacular name kayu besi (Eusideroxylon zwageri T.et.B.) is one of the the important timber in East Kalimantan (East Borneo).However, there are some problems in the regeneration of this species. The research on propagation of ulin was carried out in Treub Laboratory, Research Center for Biology. The research consist of two experiments The research consisted of two experiments.The first experiment seeds which was treated in order to stimulate the germination and the second experiment by treating the mixture between 1BA and vitamin C for stimulating root cutting.The best result of seeds germination was recorded 90%,which the seeds were treated by removing the whole seedcoat and then by exposing to the sunlight for 5 hours The seed itself began to germinate 7 weeks after sowing.Meanwhile, the control no germination et all until 28 weeks.The best result on cutting was indicated by treating with the combination of IBA 10 mg/1 and vitamin C 50 mg/1 which was achieved the highest rooting 100%,although without hormone treatment only resulted 33.3% rooting.

PERBANYAKAN IN-VITRO Gynurapseudo-china (L.) DC. (Compositae) Hoesen, Djadja Siti Hazar
BERITA BIOLOGI Vol 7, No 4 (2005)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Gynura pseudo-china (L.) DC./umbi dewa (Asteraceae/Compositae), is a species that produce tuber, the whole parts of plant especially the tuber is used as medicine. Extract of the whole plants of "umbi dewa" contains iridoids, terpenyl coumarins spirostanol steroids, pyrolizidines, purines, pyrimidines and chromanes, which has inhibit glucose cerum effect.Industrial usage of the plant requires continuos supply of materials which in turn necessitate its cultivation and planting materials.Planting materials can be produced efficiently by micropropagation technique. This objective of the study was to evaluate the culture respond to the plant growth regulator (PGR) treatments. The experiment was designed with completely randomized designed (CRD) and micropropagation result indicated that proliferation of shoots were optimum in the growth medium supplemented with N6-Benzyladenine (BA) 2 mg/l and adenine sulphate 15 mg/l, BA 4 mg/1 and adenine sulphate 10 mg/1 in combination.Inclusions chlorocholine chloride/chlormequat (CCC) and higher sucrose had positively effect on proliferation microtuber in growth medium supplemented with BA 2mg/l without adenine sulphate. Acclimatization stage and planted to the soil were successful, almost whole (95-96%) plants were survive.

PERTUMBUHAN DAN PERKEMBANGAN TUNAS Typhonium SECARA IN VITRO Hoesen, Djadja Siti Hazar
BERITA BIOLOGI Vol 8, No 5 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Study on shoots growth and development of Typhonium flagelliforme (Lodd.) Blume and T. trilobatum (L.) Schott were carried out by in vitro technique.These species produce tubers are used for cancer medication; also the whole parts of plants has been reported potential for traditional medicine. Chemicals isolated from from crude extract of whole plants of T.flagelliforme are methyl esters of hexedecanoic acid, octadecanoic acid, 9- octadecanoic acid and 9,12- octadecadienoic acid.The chemical contents inhibited/decreased the proliferation of human leukaemia cell lines in test.For medical and cosmetics industrial usage of the plants requires supply materials continously of which in turn necessitate its cultivation and planting. The planting materials can be produced efficiently by micro propagation or in vitro technique. The objective of the study was to evaluate the culture respond to the plant growth regulator (PGR) treatments effect. The experiment was designed with completely randomized designed (CRD).The study was indicated that proliferation of shoot were optimum in the growth medium was supplemented with N6-Benzyladenine (BA) 1mg/l and NAA 0,5 mg/l. Acclimatization stage and planted to the soil were successful, almost whole (90-95 %) plants were survive.

KULTUR IN-VITRO EKSPLAN RIMPANG Zingiber zerumbet van aromaticum Val Hoesen, Djadja Siti Hazar
BERITA BIOLOGI Vol 7, No 3 (2004)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Zingiberaceae species Zingiber zerumbet var. aromaticum Val. usually propagated by vegetative part of the plant. Study on the proliferation of rhizome explants through in-vitro method were conducted on this species for propagation.The explants were cultured in MS (Murashige & Skoog) composition medium in normal and half strength concentration of macro elements, supplemented with micro elements and vitamins. Plant growth regulator (PGR) cytokinin (kinetin, adenine sulphate) were added in initiation stage.Benzyl adenine (BA), thidiazuron and 2,4D (dichlorophenoxy acetic acid) added in medium as treatments to induce shoot multiplication and adventitious shoot development or rooting plants.The media for induced multiple shoot and roots (plantlets) development, supplemented with PGR BA, thidiazuron, Indole butyric acid (D3A) as treatments and activated charcoal were added as antioxidant.In general, most of cultures were indicated have positively responds to PGR treatments. The ability of culture to produce multiple shoots and roots such as in medium with BAP (8 mg/1) + thidiazuron (0.1 mg/1) + IBA (1 mg/l)-indole butyric acid would give a better opportunity of rhizome explants proliferation and plantlets development of Z. zerumbet var. aromaticum.

PERBANYAKAN DAN PENYIMPANAN KULTUR SAMBUNG NYAWA [Gynura procumbens (Lour.) Merr.] DENGAN TEKNIK IN-VITRO Hoesen, Djadja Siti Hazar
BERITA BIOLOGI Vol 5, No 4 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Sambung nyawa [Gynura procumbens(Lour.)Merr.]is one of the traditional medicine sources.The plants are not cultivated intensively in the field. One of the efforts to maintain and to propagate this species is by multiplication through tissue culture method. Nodes explants were cultured in MS normal and half strength concentration macroelements, supplemented with microelements and vitamins; combination of cytokinin BA (2 mg/1), thidiazuron (0.01 mg/1 and 0.1 mg/1) and adenine sulphate (5 mg/1).Auxin (2,4D 0.5 mg/1) in combination were added in media as treatments and activated charcoal (2 g/1) as antioxidant. Young leaves explants were also cultured in the same basic medium (MS and A MS) in treatments with cytokinin (BA and thidiazuron).The results from nodes and young leaves explant indicated that the highest number of survival cultures were obtained from combination between BA (2 mg/1) and thidiazuron (0.01 mg/1) in MS normal strength basic medium.In acclimatization stage, 100% of plantlets survived and successfully transplanted to soil medium in the field; maintenance study indicated that subculture was prolonged until 52 weeks.

KULTUR JARINGAN KUNIR PUTIH (KAEMPFERIA ROTUNDA L.) Hoesen, Djadja Siti Hazar
BERITA BIOLOGI Vol 4, No 4 (1998)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i4.1269

Shoot cultures of Kaempferia rotunda L.were established from rhizome segment.In the 1st experimen these explants were planted on Gamborg/B5 medium that supplemented with BA concentrations were (0, 0.5, 1 and 2) mg/l and/or kinetine were (0, 2 and 4) mg/l.In 2nd experiment these explants planted in Murashige and Skoog medium (MS).This medium supplemented with BA concentrations (0, 2 and 4) mg/l and/or NAA (0 and 1) mg/l.The result in the 1st experiment showed that the best proliferated shoots was from the culture that supplemented with BA 1 mg/l and kinetine 4 mg/l, while in the 2nd experiment the best proliferated shoots was from the culture that supplemented with BA 4 mg/l and NAA 1 mg/l.These shoots were then subcultured on MS liquid medium supplemented with BA (5 mg/l) and MS agar medium was supplemented with BA (2 mg/l) + 2iP (0.5 mg/l) + thidiazuron (0.01 mg/l) + NAA (0.5 mg/l).The cultures could induce the shoots number and produce the morphological plantlets for acclimatization,and acclimatization successed on soil and compost mixed medium in ratio 1:1.

PERTUMBUHAN DAN PERKEMBANGAN TUNAS KULTUR EMBRIO CARYOTA NO BECC. Hoesen, Djadja Siti Hazar
BERITA BIOLOGI Vol 4, No 1 (1997)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i1.1293

Caryota no Becc,(fish-tailpalm) is considered vulnerable palm, Its population is declining from the habitat by destructive exploitation but also to some extent by local people who remove the edible apex or 'cabbage 'as a vegetable and also obtain sago from the pith of the trunk,so kill the tree.A though its biological characteristics have not been fully studied,this species is also potentially for ornamental plant Thus conservation measures should be taken to improve the status of this threatened species. Tissue culture techniques may provide ways either to propagate or to conserve itin-vitro. Naturally Caryota no Becc.palm fruit after 20years and they can germinate more than 3 months after sowing with low germination value.In this experiment,the tissue culture response of this species are studied using the explant of embryo from (relatively) young fruit (juvenility fruit).The effect ofBA and 2,4D on the shoot growth, root growth and calluses was evaluated, while the evaluation of the effect ofactifated charcoal was discussed. The satisfactory results have not been achieved.

TEKNIK BUDIDAYA IN VITRO Eleutherine sp. (Bawang Sabrang) Hoesen, Djadja Siti Hazar
Jurnal Teknologi Lingkungan Vol. 11 No. 3 (2010)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Vegetative propagation from bulb excised of Eleutherine sp. (Iridaceae) were culturedÃ‚Â in Murashige and Skoog (MS) medium supplemented with plant growth regulatorÃ‚Â (PGR) Benzyl adenine (BA) 1 mg/l at initiation stage, BA (2 and 5) mg/l for inducedÃ‚Â shoot buds formation at multiplication stage. In this study also BA 2 mg/l and BA 2 mg/lÃ‚Â combined with naphthalein acetic acid (NAA) 0.5 mg/l were treated for rooting planletsÃ‚Â formation. Calli formation were induced with auxin PGR picloram 1 mg/l combined withÃ‚Â 2,4 dichlorophenoxy acetic acid (2,4-D) 0.5 and 1 mg/l in concentrations. The mediaÃ‚Â contained 2 mg/l cytokinin (BA) without auxin (NAA), resulted the highest shoot budsÃ‚Â formation. Rooting planlets were produced in MS medium combined with BA and NAA.MS medium contains Picloram 1 mg/l and 2,4-D 1 mg/l was optimal for frequency oncalli initiation (100%) and the largest diameter of calli also represented in cultured MSÃ‚Â medium with picloram 1 mg/l and 2,4-D 1mg/l. In acclimatization stage, 100% of planletsÃ‚Â survived and successfully transplanted to soil medium in the field.Key words: in vitro, Eleutherine sp.

PEMBENTUKAN TUNAS Lilium sp. SECARA EX VITRO DAN IN VITRO Hoesen, Djadja Siti Hazar
Jurnal Teknologi Lingkungan Vol. 10 No. 2 (2009)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Buds, planlets and bulblets formation from excised bulbscales was the preferredmethod for vegetative propagation of Lilium sp (Liliaceae). The ex vitro techniqueswith Gibberellic acid (GA3) pretreatment was induced buds formation on scalescutting which planted on sterilized sand media. Buds rised from basal scales 7days after planted. However scales untreated GA3 obtained in 35-42 after planted.In vitro methods to promote buds initiated from bulbscales explants, was inducedon media MS (Murashige and Skoog) supplemented with GA3 1 mg/l. Media forinduced buds formation, MS contained Benzyl adenine (BA) 1 mg/l and 2 mg/lincreased multiple shoots formation significantly compared cultured on mediawithout BA. Roots growth improved on media contained NAA, but the highestplanlets achieved on cultured MS media without BA. Bulblets formation obtainedon media contained higher concentration of BA (5 mg/l).

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