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Diding HP
Faculty of Medicine, Sebelas Maret University
Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology

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Perbedaan Kadar Caffeic Acid Phenethyl Ester Pada Propolis di Pasaran Wilayah Surakarta Pramono, Devina Noviani; HP, Diding; Hadinoto, Sri Hartati
Nexus Biomedika Vol 2, No 1 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: Propolis is composed of several kinds of  biological and pharmacological components that can work as immunomodulator, anti-tumor, anti-inflammation and antioxidants. This is mainly due to its chemical composition, one of which is Caffeic Acid Phenethyl Ester (CAPE). The amount and composition of its chemical components are mainly affected by several factors, some of which are the type of plant vegetation, geographical position, solvent, bees, seasonal factor, and weather. This leads to the different composition of chemical component among different brands of propolis. The same phenomenon happens in Surakarta. This research aimed to reveal either or not the different of CAPE level exist among different brand of propolis found in Surakarta’s market. Methods: This research was an observational analysis using purposive sampling method. Samples were six different brands of propolis found in Surakarta’s market. The samples were divided into 6 groups which were P1, P2, P3, P4, P5 and P6. Each group was measured five times. The measurement of CAPE level of each sample was done using UV Vis spectrophotometer with wave length of 700 nm using Prussian Blue method. Data analysis was done using one way Anova statistical test with SPSS 17.00 for Windows program.   Results: The average level of CAPE found in propolis samples were P1 =11.531±0.078 µg/ml; P2 =5.438±0.106 µg/ml; P3 =12.541±0.052 µg/ml;  P4 =9.036±0.081 µg/ml; P5 =10.593±0.043 µg/ml; and P6 =10.366±0.133 µg/ml with p value is below 0.001 (p < 0.001). Conclusions: There was a difference of CAPE level among propolis found in the market area of Surakarta. Keywords: CAPE, Propolis
Perbedaan Kadar Caffeic Acid Phenethyl Ester antara Propolis yang Diekstraksi dengan Etanol dan Air Hastuti, Riani Dwi; HP, Diding; Hartati, Sri
Nexus Biomedika Vol 2, No 2 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

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Background: Propolis  is a natural product derived from plant resins which is collected by honeybees. One of the major components of propolis is Caffeic Acid Phenethyl Ester (CAPE). The use of various kinds of solvents in propolis extraction effects CAPE level. The aim of this research was to know the difference levels of CAPE in ethanol extract propolis and water extract propolis. Methods: This study was experimental research with post test only group design. Samples were raw propolis taken from bee farm in Gejen RT 03 RW 02, Kerjo, Karanganyar. Samples were extracted with maceration method using ethanol and water as solvents. Both of ethanol extract propolis and water extract propolis were divided into five samples. CAPE levels were determined by UV Vis spectrophotometer at wave length of 700 nm using Prussian Blue method. Data were analyzed using independent sample t test. Results: The result showed that the average of CAPE level in propolis extracted with ethanol was 12.26 ± 0.658 µg/mL, while the average of CAPE level in propolis extracted with water was 5.564 ± 0.332 µg/mL. Conclusions: There was no significant difference between propolis extracted with ethanol and water. Keywords: CAPE, ethanol extract propolis, water extract propolis.
Pengaruh Probiotik terhadap Gambaran Histologis Mukosa Usus pada Mencit Balb/C Model Alergi HP, Diding; Listyaningsih S., Endang; Subijanto, AA
Jurnal Kedokteran YARSI Vol 16, No 1 (2008): JANUARI - APRIL 2008
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33476/jky.v16i1.219

Abstract

The mechanisms by which probiotic bacteria affect the immune system are unknown yet, but many of them are attributed to an increase in the innate or in the acquired immune response. The aim of this study was to examine the effect of the probiotic on the histology structure of intestinal layer in a mice model of allergy. Male Balb/C mice were sensitized and challenged intra peritoneally (i.p) with ovalbumin(OVA). Mice were immunized i.p. on days 0 and 7 with 2.5 mg of OVA adsorbed to 7.75ml of Aluminum hydroxide gel. OVA challenges (10 mg in l0 ml of PBS) were administered orally on days 8, 9, 10, 11, 12 and 13, with mice being sacrificed on day 14. This probiotic was orally administered to Balb/C mice. Intestinal microscopic slides were prepared using parafin method and stained with hematoxylin eosin staining. The data were analyzed by Kruskal-Wallis, followed by MannWhitney Test. The results showed that probiotic can minimized inflammation of intestinal layer in the experimental animal, not significantly different with 3rd generation of antihistamine.
Pengaruh Ekstrak Daun Ceremai (Phyllanthus acidus [L.] Skeels) terhadap Kadar IgE pada Mencit Model Alergi Subijanto, AA; HP, Diding
Jurnal Kedokteran YARSI Vol 16, No 1 (2008): JANUARI - APRIL 2008
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (217.868 KB) | DOI: 10.33476/jky.v16i1.220

Abstract

It was reported in previous studies that pharmacological activities were identified in various compounds extracted from Phyllanthus Acidus (PA) such as anti-inflammatory, antilipoxigenase, anti-allergic and cyclooxigenase inhibitor. It is also acknowledged that Ig E serum level is normally low and usually increase in allergic conditions. This study was aimed to determine the effect of PA leaf extract on Ig E level in mice model of allergic asthma. Male Balb/C mice were sensitized and challenged intraperitoneally (i.p) with ovalbumin(OVA). Mice were immunized i.p. on days 4 and 20 with 2.5 mg of OVA adsorbed to 7.75ml of Aluminum hydroxide gel. OVA challenges (10 mg in l0 ml of PBS) were administered aerosolly on days 26, 29, 32, 39 and 46, with mice being sacrificed on day 47. Ig E level in serum was determined using ELISA kits (Alpha Diagnostic, USA) according to the manufacturer's instructions. A one-way analysis of variance (ANOVA) with LSD post hoc analysis was used to determine significant differences. Results were expressed as mean + SEM, and values of p < 0.05 were considered statistically significant. The results showed that Ig E level in the negative control group was 50.13+33.40 ng/ml, PA leaf extract was 51.17+26.15 ng/ml, and positive control group was 48.97+33.50 ng/ml while OVA group was 152.89+58.00 ng/ml (p >0.05). It was concluded that PA leaf extract could minimize Ig E level, not significantly different with 3rd generation of antihistamine.
Co-Authors AA Subijanto Devina Noviani Pramono Listyaningsih S., Endang Riani Dwi Hastuti Sri Hartati Sri Hartati Hadinoto