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BIODIVERSITY OF ARBUSCULAR MYCHORRIZAL FUNGI (AMF) ON POTATOS RHIZOSPHERE AND IT POTENTIAL AS BIOFERTILIZER Yelianti, Upik; kasli, Kasli; Kasim, Musliar; Husin, Eti Farda
Sainstek Vol 12, No 1 (2009): Vol. XII No. 1, September 2009
Publisher : Lembaga Penelitian Universitas Negeri Padang

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Abstract

AMF as the biofertilizer on several crops  has been reported, but not so much the information about biodiversity of AMF on potatos rhizosphere and its potential to colonize that crop. The research about the biodiversity of AMF has been done in laboratorium  Biology in Faculty of Agriculture of Andalas University and in laboratorium Biotecnology of Agriculture Faculty of Jambi University from November 2004 till April 2005. The sample of soil have been collected from  rhizosphire of potatos plant in Alahan Panjang, West Sumatera, and then the AMF spore are isolated and identification based on morphology and size of spores. Inoculation of single and multi spores to potatos root to see the structure of colonization and percentage of colonization. The result of identification of AMF spores show that there are many kind of spores on rhizosphere of potatos plant and the spores are dominated by: Glomus, Acaulospora, Scutellospora, Gigaspora, and Enterophospora. Unfortunately, the inoculation of single spore has no good effect to the structure of colonization and the potatos plant not vigorous and easy to be severe of stem desease. But, inoculation with multi spores show that the tipe of colonization on potatos root have the coil hyphal and intracellular vesicular. The same result also indicated that colonization with active propagule (mycelium, spores, and infected root) have the coil hyphal and intracellular vesicular. The species of AMF that have the typical characteristic is assumed as Gigaspora sp.. Key words :    AMF, potatos rhizosphere, biofertilizer
Senyawa Phenolik Akar Pisang CV. Kepok (Musa acuminata) yang Diinduksi dengan Fungi Mikoriza Arbuskular Indigenus PU10-Glomus sp 1 terhadap Penyakit Darah Bakteri Suswati, Suswati; Habazar, Trimurti; Husin, Eti Farda; Nasir, Nasril; Putra, Dedi Prima; Taylor, Peter
Jurnal Natur Indonesia Vol 13, No 3 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (136.413 KB) | DOI: 10.31258/jnat.13.3.207-213

Abstract

Cooking banana (Musa acuminata) cv. Kepok is the most susceptible to Blood disease bacterium (BDB) infection.From previous study revealed the best isolate indigenous Arbuscular Mycorrhiza Fungi-Pasar Usang 10 (PU10-Glomus sp 1) could induce cv.Kepok resistance to BDB in green house and field experiment. The AMF could changethe phenolic compound in root plant. This objectives were to measure the root phenolic compound and bioassayto BDB. The 50 grams fresh inoculant PU10-Glomus sp 1 were applicated to banana root plants 60 days old with 6levels time course: 12; 24; 36; 48; 72; 92 hours and control (without PU10-Glomus sp 1). The root methanolicextraction followed to Echeverri et al., (2002) methode with vacuum concentration of the filtrate and partitioninginto ethyl acetate revealed the presence of an antibacterial compound as detected by TLC (Thin LayerChromatography), assay phenolic contained by Spectrofotometer UV-Vis 1700. PharmaSpec. Shimadzu andbioassay using BDB. Nine antibacterial compounds rose from root banana seedling colonized by PU10-Glomus sp1 in 12 hours after applicated (haa) ; 24; 36 and 48 haa. They were with Rf values of 0.16; 0.17; 0.19; 0.26; 0.32; 0.37;0.71; 0.80 and 0.83 on silica plates run in hexane:ethyl acetate (1:2 v/v) and control contained only 0.05 and 0.28.These compounds produced fluorescens which was bright yellow green spots and purple and have antimicbrobialproperties to BDB.
EFEKTIVITAS BERBAGAI FUNGI MIKORIZA ARBUSKULAR INDIGENUS TERHADAP SERAPAN HARA P DAN PERTUMBUHAN TANAMAN JARAK PAGAR (Jatropha curcas L.) Muzakkir Muzakkir; Eti Farda Husin; Agustian Agustian; Auzar Syarif
Jurnal Solum Vol 7, No 2 (2010): JURNAL SOLUM
Publisher : Jurusan Tanah Fakultas Pertanian Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (190.345 KB) | DOI: 10.25077/js.7.2.137-143.2010

Abstract

Utilization of arbuscular mycorrhizae fungi (FMA) is an alternative way to improve soil fertility. Therefore, it must be developed by studying deeply the effectivity of the FMA. This is important due to either its ability to associate with almost all of vegetation family in terrestrial ecosystems or its ability to increase plant growth rate, seedlings quality, and crop yield, especially on critical land. A research about effectivity of variously indigenous arbuscular mycorrhizae fungi on P-sorption and Jatropha growth was conducted at laboratorium and glasshouse Agriculture Faculty, Andalas University. This research was aimed to gain the most effective isolate of arbuscular mycorrhizae fungi on growth of and P-sorption by Jatropha (Jatropha curcas L.) plant on critical land. Single isolat such as Glomus sp2, Acaulospora sp1, Gigaspora sp1, and compound isolate M7 (Glomus sp2,+ Acaulospora sp1 + Gigaspora sp1) significantly affected plant height, canopy dry matter, infection percentage and intensity, and P-sorption. M7 Compound isolate was the most effective innoculum. By this isolate, height of Jatropha seedlings was 27.5 cm, canopy dry matter 5.9 g, infection percentage 78.5%, infection intensity was 49.1, and P-sorption by canopy was 1.6 g for each seedling. Keywords: Effectiveness of FMA
PERUBAHAN HARA DAN MIKROORGANISME PADA ULTISOL AKIBAT KASCING YANG BERBEDA Oktanis Emalinda; Eti Farda Husin; Dina Puspita Rini
Jurnal Solum Vol 2, No 2 (2005): Jurnal SOLUM
Publisher : Jurusan Tanah Fakultas Pertanian Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (59.198 KB) | DOI: 10.25077/js.2.2.%p.2005

Abstract

This research was conducted in Soil Drying Room at Faculty of Agriculture Andalas University. Analysed in Soil Biology, Soil Chemic and Plant test and Disease Laboratories from August 2006 to February 2007. Aim of this research is to know the effect of substrate of vermicompost on population of soil microorganism on Ultisol. This experiment were designed on Complete Randomized Block Design in three treatment and three replicate. The treatment were different substrate of vermicompost, consisted of : without vermicompost, 10 ton/ha vermicompost with rice strow substrate and, 10 ton/ha vermicompost with dust wood. The result was showed, that vermicompost with rice strow could increase the growth rate of microorganism 320,14 % of fungi and 123,3 % bacteri. Used that this vermicompost can also increase soil nutritions (0,22 %, variable P 0,7 %; K-dd 0,04 me/100 g, Ca-dd 0,13 me/100 g and Mg-dd 0,08 me/100. Key word : vermicompost
INTRODUKSI FORMULA FUNGI MIKORIZA ARBUSKULA DARI RIZOSFER PISANG PADA BIBIT PISANG UNTUK PENGENDALIAN PENYAKIT DARAH BAKTERI (RALSTONIA SOLANACEARUM PHYLOTYPE IV) Yefriwati, Yefriwati; Habazar, Trimurti; Husin, Eti Farda
JURNAL AGROTROPIKA Vol 16, No 1 (2011): Agrotropika Vol.16 No.1 2011
Publisher : JURNAL AGROTROPIKA

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Abstract

Blood disease bacteria (BDB) caused by Ralstonia solanacearum Phylotype IV (Pseudomonas solanacearum) is one of the most important diseases on banana. Using biological agents such as arbuscular mycorrhizal fungi (AMF) to control BDB is still not maximal result, based on that need to search a potensial indigeneous AMF specific location. The aim of this experiment were to study the stability of formulated AMF indigenous to control BDB and to increase growth of banana seedlings. This research was arranged by Factorial in Randomized Complete Design (RCD) on green house experiment with 5 replicate. The treatment consist of 2 factors : 1) enrichment of carrier of AMF, sand with rock phosphate (0, 10, 20 and 40 %). 2) incubation periode (0, 1 and 2 months). Banana seedlings were intruduced with formulated AMF at planting date. Two month old banana seedlings were inoculated with Ralstonia solanacearum Phylotype IV. The parameter were observed include: incubation periode, disease insidence, disease severity, discoloration of pseudostem, population density of Ralstonia solanacearum Phylotype IV, colonisation degree of AMF on banana root, spore density on rhizosphere, growth of banana seedlings.The results showed that all formulated AMF introduced on banana seedlings reduced BDB development and increase banana growth compare with control plants, especially the formulated AMF enriched with 30 % rock phosphate.Key Words: arbuscular mycorrhizal fungi, blood disease bacteria, biological controll agents, banana
Potential Selection of Arbuscular Mycorrhizal Fungi (AMF) Indigenous Ultisols through the Production of Glomalin Eddiwal, .; Saidi, Amrizal; Lenin, Ismon; Husin, Eti Farda; Rasyidin, Azwar
JOURNAL OF TROPICAL SOILS Vol 19, No 3: September 2014
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2014.v19i3.181-189

Abstract

The arbuscular mycorrhizal fungi ( AMF ) with plants able to increase the capacity of plants to absorb nutrients and water from the soil. Recently, research was indicated that AMF hyphae containing glomalin as a glycoprotein that serves to unify the dispersed soil particles. The content of glomalin in soil is positively correlated with soil aggregate stability. The research potential of AMF species indigenous of Ultisol Darmasraya District of West Sumatra and glomalin production in experimental pots of sterile sand medium has been carried out. The purpose of this study was to determine the diversity of AMF species on Ultisol and to seeking indigenous AMF isolates that had the best glomalin production capability. AMF spores were isolated and identified from the rhizosphere soil of corn in Ultisol. AMF species that had been identified experimentally were tested in culture medium pot of sand and zeolite (w / w 1:1) using corn crops. The results found nine of the AMF species indigenous of Ultisol Darmasraya, namely Acaulospora scrobiculata, Glomus etunicatum, Glomus luteum, Glomus mosseae, Glomus verruculosum, Glomus versiforme, Scutellospora gregaria, Scutellospora heterogama and Gigaspora sp. AMF species that showed better colonization ability in corn was G. luteum, G. verruculosum and G. versiforme. All three species produced glomalin significantly higher than the other species, i.e. 1.29 mg g-1; 1.17 mg g-1; 1.15 mg g-1, respectively. [How to Cite: Eddiwal, A Saidi, I Lenin, EF Husin and A Rasyidin. 2014. Potential Selection of Arbuscular Mycorrhizal Fungi (AMF) Indigenous Ultisols through the Production of Glomalin. J Trop Soils 19: 181-189. Doi: 10.5400/jts.2014.19.3.181]