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PRODUKSI ENZIM SELULASE TERMOSTABIL DARI BAKTERI NG2 MENGGUNAKAN BERBAGAI SUMBER SELULOSA ASAL LIMBAH PERTANIAN DAN PERKEBUNAN (Production of Thermostable Cellulase Enzyme by NG2 Bacteria Using Various Cellulose Sources from the Agriculture Waste) Rahmad Fani Ramadhan; M. Montesqrit; Yetti Marlida
Jurnal Ilmu dan Teknologi Peternakan Vol. 8 No. 2 (2020)
Publisher : Fakultas Peternakan, Universitas Hasanuddin, Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jitp.v8i2.8171

Abstract

This experiment aimed to determine the best cellulose sources from the agricultural by product on the production of the thermostable cellulase enzymes by thermophilic bacteria (NG2) and also to find out the best concentration level of the selected sources of cellulose. The experiment was conducted in two stages using a completely randomized design (CRD). In phase 1, four cellulose sources were used, namely A: corn cobs, B: rice straw, C: coconut fiber, and D: oil palm trunk, in which the replication for each source was five. In phase 2,  the best sources of cellulose from the phase 1 was used at four different levels of concentration, i.e., A: 2%, B: 3%, C: 4%, and D: 5%, in which replication for each concentration was five. The result of phase 1 showed that the corn cobs was the best cellulose source  in producing cellulase enzyme. This was indicated by the activity of 0.329 U/ml, the enzyme protein of 0.0328 mg/ml, and the specific activity of 10.165 U/mg, which were significantly higher (P<0.01) than those of other cellulose sources. The results of phase 2 showed that the concentration of 4% (w/v) had the highest (P<0.01) specific activity of 44.002 U/mg  compared with those of the other concentrations. In conclusion, the highest production of cellulase enzyme using thermophilic bacteria (NG2) was obtained at the concentration of 4% (w/v) using the corn cobs as the cellulose source.
Pengaruh Suhu dan Lama Penyimpanan terhadap Stabilitas Minyak Ikan dan Mikrokapsul Minyak Ikan Montesqrit Montesqrit; R Ovianti
Jurnal Peternakan Indonesia (Indonesian Journal of Animal Science) Vol 15, No 1 (2013): Jurnal Peternakan Indonesia
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpi.15.1.62-68.2013

Abstract

Fish oil microcapsules could be stored long longer than fish oil. Storage of the fish oil microcapsules to be more stable and effective to note factors such as temperature and storage time. The purpose of this study was to determine the effect of temperature and storage time on the stability of fish oil (MI) and microencapsulated fish oil (MMI). The research method used was a completely randomized design method factorial 2 x 3 and 3 replications for each MI and MMI. Factor A (storage temperature: refrigerator temperature and room temperature) and factor B (storage time: 15, 30 and 45 days). The variables measured were peroxide, TBA numbers and the total number of oxidation. The results of this study found that the more stable MMI stored in the refrigerator compared to room temperature and the MMI is more stable compared with MI. Temperature and storage time is best for the oxidative stability of microencapsulated fish oil and fish oil are refrigerator temperature and storage time of 45 days.
Pengaruh Pemberian Mikrokapsul Minyak Ikan dalam Ransum Puyuh terhadap Performa Produksi Sestilawarti Sestilawarti; Mirzah Mirzah; Montesqrit Montesqrit
Jurnal Peternakan Indonesia (Indonesian Journal of Animal Science) Vol 15, No 1 (2013): Jurnal Peternakan Indonesia
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpi.15.1.69-74.2013

Abstract

An experiment was conducted to determine the effect of feeding fish oil microcapsule on quail performances.  200 quail layers were randomly allocated into 20 pens.  A randomized completely design was used in this study with 0, 2%, 4% and 6% fish oil microcapsule in diet.  Variable measured were feed consumption, egg production, egg weight, mass of egg and feed conversion. Data were analized by CRD and Duncan Multiple Range Test (DMRT).  Results of the experiment indicated that the inclusion of fish oil microencapsule in laying quails diet did not affect the production performances (feed consumption, egg production, egg weight, mass of egg and feed conversion).
Isolasi dan Karakterisasi Selulase dari Trichoderma Viride dan Rhizopus Spp dengan Substrat Jerami Padi Montesqrit Montesqrit
Jurnal Peternakan Indonesia (Indonesian Journal of Animal Science) Vol 12, No 2 (2007): Jurnal Peternakan Indonesia
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (896.508 KB) | DOI: 10.25077/jpi.12.2.112-123.2007

Abstract

The objectives of this study were to isolated and produce cellulase from soil molds and to characterize the enzyme. The strains of mold isolated from soil, namely Rhizopus spp and one cultured mold. Trichoderma viride, was used to produce the enzymes. Medium for enzyme production consisted of NH4NO3, KCl, FeSO4.7H2O, MgSO4.7H2O, CuSO4.2H2O and rice straw as a sole source of carbon (pH : 5.45). Culture was done at 28oC for 5,8,11 and 14 days with shaking at 150 rpm. Using this method cellulase production was optimum at 14 days with substrat concentration of 1.5% for T. viride culture. However the optimum production of cellulase for Rhizopus spp culture were three days shorter than culture of T.viride with substrat concentration of 1.5 and 1% respectively. Temperature and pH optimal activity of cellulases were as follow : cellulase from T.viride at temperature 60oC and pH 5 and cellulase from Rhizopus spp at temperature 50oC and pH 5. While temperature and pH stability of cellulases were as follow : cellulase from T. viride at temperature 30 - 80oC and pH 3 - 7 and cellulase from Rhizopus spp at temperature 30 - 80oC and pH 3-6. It was concluded that molds can grow on rice straw as a sole source of carbon produce cellulases.
PENGARUH PENAMBAHAN DAUN MINDI (Melia azedarach Linn) TERHADAP KUALITAS JAGUNG PIPILAN SELAMA PENYIMPANAN Montesqrit Montesqrit; Harnentis Harnentis; Sri Yana
Pastura : Jurnal Ilmu Tumbuhan Pakan Ternak Vol 8 No 2 (2019): Pastura Vol. 8 No. 2 Tahun 2019
Publisher : Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (824.339 KB) | DOI: 10.24843/Pastura.2019.v08.i02.p02

Abstract

This study aims to evaluate how the effect of the addition level of neem leaf flour (Melia azedarach Linn) on the quality of shelled corn during storage. The research material used was neem leaves, shelled corn, storage containers and analytical equipment. Neem leaves are given in the form of flour with a size of 60 mesh while corn used in shell corn with water content and aflatoxin content before storage are 20% and 110 ppb respectively. Shelled corn was used as much as 1 kg for each treatment and stored in modified woven bags for 4 weeks. The research method is an experimental method using a completely randomized design (CRD), with 4 treatments and 4 replications. The treatments were the percentage of neem leaf flour addition in shelled corn, the four treatments were treatment A (0% neem leaf flour), B (2.5% neem leaf flour), C (5% neem leaf flour), and D ( 7.5% neem leaf flour). Parameters measured were percentage of damaged seeds, percentage of moldy seeds, moisture content and aflatoxin content. The results of the study showed that the treatment had a highly significant effect (P<0.01) on% of damaged seeds, % of moldy seeds, and aflatoxin content on shelled corn during storage. The administration of neem leaf flour at 2.5% level was able to maintain the quality of shelled corn during storage, where the aflatoxin content could decrease from 110 ppb before storage to 34,25 ppb after being stored for 4 weeks. Keywords: neem (Melia azedarach Linn), shelled corn, storage, aflatoxin
Ipteks bagi Masyarakat Kelompok Tani Tanjung Saiyo dan Peternak Muda Ayam Kampung Super di Nagari Lolo Kecamatan Pantai Cermin Kabupaten Solok Fitrini Fitrini; Montesqrit Montesqrit; Wazir Wazir
Warta Pengabdian Andalas Vol 24 No 1 (2017): Warta Pengabdian Andalas
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) Universitas Andalas

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Abstract

Nagari lolo is an agricultural area that is dominated by rice fields so the main occupation of this village community is farming. Side jobs such as raising cattle and poultry. Partner devotion is Tanjung Saiyo farmer groups (rice farming and raising cattle) and breeder/ Ermita (raising chicken super). The purpose of this community service activities performed on both partners are providing sustenance to the practical science to both partners ranging from the technical aspects of cultivation, utilization of waste straw into cattle feed, chicken feed precise formulation, business planning until meat processing. Method of execution by means of education, discussion, demonstration, training and mentoring. Results devotion has been carried out as planned, by partner I: manufacture of straw fermentation, the manufacture of a feed processing, repair cowshed GFP standards and treatment of foot disease in cattle, cattle business plan development and manufacture of processed beef into nuggets, while the partner II : nursery and chicken hatching machine manufacture, manufacture of feed formulation chicken super precise, manufacture super chicken business plan, and the manufacture of processed chicken meat into meatballs. The success of visible activity of participation and cooperation given by both partners.
TEKNOLOGI PENANGANAN PAKAN DENGAN SILASE RANSUM KOMPLIT BERBASIS LIMBAH TEBU UNTUK MENINGKATKAN PRODUKTIVITAS TERNAK KAMBING PERAH DI KABUPATEN AGAM Montesqrit montesqrit; Rusmana Setianingrat; Afrizal Afrizal
Jurnal Pengabdian Warta Andalas Vol 24 No 3 (2017): Warta Pengabdian Andalas
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) Universitas Andalas

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Abstract

Usaha peternakan kambing perah cukup berkembang di Kabupaten Agam salah satunya kelompok tani Maju Mandiri di Nagari Sungai Pua, Kecamatan Sungai Pua. Pada saat ini memiliki 11 anggota peternak kambing etawa dengan jumlah kambing 70 ekor (50 ekor betina, 3 ekor pejantan dan 20 ekor anak). Secara teknis telah banyak dibina oleh dinas Peternakan Kota Bukittinggi dan Kabupaten Agam dalam manajemen produksi dan manajemen pemasaran, akan tetapi masih kurang dalam manajemen pakan. Tujuan pengabdian masyarakat ini adalah untuk menerapkan iptek di bidang teknologi penanganan pakan dengan silase ransum komplit. Metode yang dilakukan adalah identifikasi bahan baku pakan di lokasi peternak tersebut, penyusunan formulasi ransum, pembuatan silase ransum komplit selama 3 minggu dan aplikasinya pada ternak. Hasil penelitian yang didapatkan banyak bahan pakan lokal yang berpotensi untuk dimanfaatkan seperti daun paitan (Thitonia diversifolia), terung afkir, daun kembung dan daun mikania. Hasil silase ransum komplit yang dibuat kualitasnya bagus yaitu beraroma asam, tekstur lembut, warna agak kehijauan dan tidak berjamur. Silase tersebut sangat disukai oleh ternak dan produksi susu meningkat setelah ternak mengkonsumsinya. Kesimpulannya adalah teknologi penanganan pakan dengan silase ransum komplit sangat diminati oleh kelompok tani tersebut karena teknologinya mudah, penanganan pakan lebih praktis, disukai oleh ternak dan dapat meningkatkan produktivitas.
PEMBERDAYAAN MASYARAKAT DALAM MEMANFAATKAN TANAMAN PAITAN (Thitonia diversifolia) SEBAGAI PAKAN DAN PUPUK ORGANIK DI BATAGAK KECAMATAN SUNGAI PUA KABUPATEN AGAM Montesqrit Montesqrit; Yuliaty Shafan Nur
Jurnal Pengabdian Warta Andalas Vol 25 No 1 (2018): Warta Pengabdian Andalas
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) Universitas Andalas

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Abstract

Thitonia diversifolia are shrub plants that grow very fast and can reach up to 2 meters high. Theseplants are very much found in Nagari Batagak, Sungai Pua District, Agam Regency. The plant has notbeen used properly, which is usually cut down and then discarded. Not many people there use it as liquidor solid organic fertilizer or as animal feed ingredients. The purpose of this service is to empower thecommunity to utilize the local potential as organic feed and fertilizer. The method used is in the form ofcounseling and demonstration and application of the product. Counseling and demonstration carried outis the manufacture of solid and liquid organic fertilizers from linkages, utilization of linked plants as amixture of animal feed ingredients in the form of complete ration silage. The application carried out isthe application of the organic fertilizer to vegetable plants compared to chemical fertilizers and theapplication of complete ration silage to livestock. The application of solid and liquid organic fertilizersfrom paitan plants to vegetable mustard plants obtained morphological results (size, average weight,color, visualization, texture) and harvest age were not significantly different between organic vegetablesfertilized with organic fertilizers compared to fertilized organic vegetables with chemical fertilizers.Costs incurred for processing organic vegetables are cheaper than organic vegetables. The use of linkedplants as feed can be used up to 25% in complete ration silage.
PELATIHAN PEMANFAATAN LIMBAH SAYURAN UNTUK TERNAK RUMINANSIA DI KAWASAN PERKEBUNAN TEBU RAKYAT NAGARI TALANG BABUNGO Montesqrit Montesqrit; Adrizal Adrizal; Irsan Ryanto
Jurnal Pengabdian Warta Andalas Vol 25 No 4 (2018): Warta Pengabdian Andalas
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) Universitas Andalas

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Abstract

Tujuan pengabdian kepada masyarakat ini adalah untuk meningkatkan pendapatanpeternak sapi dan kerbau dengan memanfaatkan limbah tebu yang dipadukan dengan limbahsayuran yang banyak tersedia di lokasi pengabdian. Pengabdian dilakukan dengan transferIPTEK pemanfaatan limbah tebu dan limbah sayuran yang dilakukan dengan memformulasikanransum dengan mempertimbangkan biaya perolehan bahan-bahan pakan, kandungan gizibahan-bahan pakan yang akan digunakan, serta kebutuhan gizi ternak yang dipelihara, sehinggakebutuhan gizi ternak terpenuhi dengan biaya serendah mungkin. Formulasi ransum tersebutdilakukan dengan linear programming. Hasil formula ransum diimplementasikan pada usahapeternakan milik Koperasi Serba Usaha Ekonomi Desa (KSU-ED) Tabek sebagai percontohan.Proses implementasi dilakukan dengan melibatkan peternak yang tergabung dalam koperasi.Tahapan implementasi dimulai dengan pengumpulan bahan-bahan pakan yakni pucuk tebu,ampas tebu, molases, limbah sayuran berupa kubis, sawi dan ubi jalar serta bahan-bahan pakanlainnya sesuai dengan hasil formulasi ransum. Selanjutnya dilakukan proses produksi ransumkomplit pada pabrik pakan milik KSU ED Tabek. Ransum tersebut disimpan dalam bentuksilase dengan menggunakan drum plastik yang dilapisi plastik polietilen. Silase disimpanselama 21 hari, selanjutnya diberikan kepada kerbau milik KSU ED Tabek. Pemberian kepadakerbau diberikan selama 3 bulan, konsumsi, pertambahan berat badan dicatat selamapemeliharaan. Pengaruh pemberian pakan tersebut dievaluasi terutama pengaruhnya terhadapefisiensi ransum dan pendapatan peternak
Pengembangan Usaha Penetasan Telur Itik “Regra Hatchery” di Nagari Lubuk Tarok Kabupaten Sijunjung Montesqrit Montesqrit; Haris Suryamen; Aprizal Aprizal
Warta Pengabdian Andalas Vol 28 No 2 (2021)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jwa.28.2.115-121.2021

Abstract

The RegRa farm and hatchery in Lubuk Tarok, Sijunjung Regency, West Sumatra, has started a duck egg hatchery business. The hatchery business is not following good standards and has not brought high profits. This is because the hatchability of the eggs produced is still low; it takes time every day to turn the eggs over; the size of ducklings is many small, and the fertile eggs to be hatched are still purchased from other places. The community engagement team gave the solution for this problem: renovating the hatching machine with automatic egg turning, repairing the enlargement cage, renovating the cage, and caring for the ducklings. The solutions offered were carried out well by partners. As a result, the hatchability of eggs increases, the ducklings sold are healthy and agile, and there is a desire to raise ducklings to produce their fertile eggs in the future.