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All Journal Pharmaciana: Jurnal Kefarmasian Buletin Penelitian Tanaman Rempah dan Obat Biomedical Engineering Buletin Penelitian Tanaman Rempah dan Obat
Wahyu Widowati
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Medicine & Pharmacology

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Antioxidant properties of spice extracts Wahyu Widowati
Biomedical Engineering Vol 1, No 1 (2015): BIOMEDICAL ENGINEERING
Publisher : Biomedical Engineering

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (243.221 KB)

Abstract

Objective : This study was conducted to determine the antioxidant activities of spice extractsincluding clove (Syzygum aromaticum L.), Indonesian cassia (Cinnamomum burmanni (C. Nees& T.Ness)), coriander (Coriandrum sativum L), nutmeg (Myristica fragrans Houtt), java cardamom(Amomum compactum Soland. Ex maton).Methods : This research was to evaluate antioxidant activities including 1,1-diphenyl-2-picrylhydrazyl(DPPH) scavenging activity, the value of superoxide dismutase (SOD), and total phenoliccontent.Result : The highest DPPH activity is clove and Indonesian cassia extracts with IC50 value 4.16μg/ml and 5.46 μg/ml respectively. The highest SOD value are Indonesian cassia extract (9.1432U/ml) at 500 μg/ml, 7.0045 U/ml at 125 μg/ml and 4.6751 U/ml at 31,25 μg/ml. Clove extract was thehighest of phenolic content (188.35 μg/mg eugenol equivalent).Conclusion : Indonesian cassia extract have high antioxidant activities both DPPH scavenging andSOD activities. Clove extract contain the highest eugenol compared with Indonesian cassia,coriander, nutmeg and java cardamom.
The ethanol extract of the bastard cedar (Guazuma ulmifolia L.) as antioxidants Sijani Prahastuti; Meilinah Hidayat; Stella Tinia Hasiana; Wahyu Widowati; Wahyu Setia Widodo; Rr. Anisa Siwianti Handayani; Rizal Rizal; Hanna Sari Widya Kusuma
Pharmaciana Vol 10, No 1 (2020): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (29.278 KB) | DOI: 10.12928/pharmaciana.v10i1.13636

Abstract

Guazuma ulmifolia, or commonly known as the bastard cedar, has many pharmaceutical activities and is, therefore, claimed as a source of various plant-based medicines. This reserach was purposed to identify the antioxidant activities of the ethanolic extract of G. ulmifolia (EEGU) by phytochemical screening assay, total flavonoid and total phenolic testing, and comparative analysis between the antioxidant activities of EEGU and epicatechin. The qualitative phytochemical screening assay of EEGU detected the availability of phenols, flavonoids, alkaloids, tannins, and terpenoids, but not saponins and triterpenoids. Meanwhile, the total phenolic content was 32.24 µg GAE/mg extract, and the total flavonoid content decided using aluminum chloride reagent with quercetin standard, was 6.48 µg QE/mg extract. The role of antioxidants examined by FRAP, DPPH, H2O2, and ABTS assays. These assay are proved that the IC50 values of EEGU are higher than those of epicatechin. For DPPH scavenging, H2O2 scavenging, and ABTS reduction activities, EEGU resulted IC50 45.70 μg/mL, 162.93 μg/mL, and 35.96 μg/mL, while epicatechin only yielded IC50 0.56 μg/mL, 57.91 μg/mL, and 16.74 μg/mL respectively. Otherwise, the highest reduction in FRAP activities were shown at  50 μg/mL concentration  of epicatechin and EEGU were 236.33 and 202.71 µM Fe (II)/µg respectively. Based on these results, EEGU is concluded as an active natural product because it exhibit antioxidant activities.
POTENCY OF ANTIOXIDANT, ANTICHOLESTEROL AND PLATELET ANTIAGGREGATION OF BLACK TEA (Camelia sinensis ) Wahyu Widowati; Tati Herlina; Hana Ratnawati; Tjandrawati Mozef; Victor Immanuel
Buletin Penelitian Tanaman Rempah dan Obat Vol 22, No 1 (2011): BULETIN PENELITIAN TANAMAN REMPAH DAN OBAT
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v22n1.2011.%p

Abstract

Epidemiological studies have shown an in-verse correlation betweendiets rich in po-lyphenols, flavonoids and reduced risk of cardiovasculardisease. These associationswere mainly ascribed to the antioxidant, antiaggregation and anticholesterol capa-city of polyphenols. This association has been explained that atherogenesis is ini-tiated by hypercholesterol, lipid peroxida-tion and hyper aggregation platelet. The research was carried out to evaluate anti-oxidant, anticholesterol and antiaggrega-tion activities of methanol extract and frac-tions of black tea (Camelia sinensis). To evaluate antioxidant activity of methanol extract and fractions were compared  with (-)-Epigallocatechine 3-gallate (EGCG), an-ticholesterol activity were compared with simvastatin and antiaggregation activity were compared with aspirin. Antioxidant activity using the 1,1-diphenyl-2-picryl-hy-drazyl (DPPH) free radical scavenging acti-vity at two concentrations 100 µg/mL, 50 µg/mL and anticholesterol was cholesterol oxidase activity at two concentrations 0.005 µg/mL, 0.0025 µg/mL, the antiag-gregation  activity  used epinephrine (EPN)agonist at two concentrations 300 μM, 75 μM and one concentration 37.1 µg/mL of methanol extract and fractions of black tea. To know the difference of treatment, the data were analysed using Analysis of Variance (ANOVA) and be continued Dun-can’s new multiple range test. The results showed that DPPH scavenging activity were high (88.59-93.556%), the anticho-lesterol showed high activity (93.663-97.434%) and the antiaggregation sho-wed low up to high activity (79.967-4.31%). Using Duncan’s new multiple ra-nge test showed that anti-oxidant activity were not different among extract and fractions of black tea,the highest anti-cholesterol activity was black tea extract 0.005 µg/mL (97.434%) and the highest antiaggregation activity was ethyl acetate fraction on 75 μM EPN agonist (4.310 %), water fraction 37.1 µg/mL with 300 μM EPN agonist (21.833%).
POTENCY OF ANTIOXIDANT, ANTICHOLESTEROL AND PLATELET ANTIAGGREGATION OF BLACK TEA (Camelia sinensis ) Wahyu Widowati; Tati Herlina; Hana Ratnawati; Tjandrawati Mozef; Victor Immanuel
Buletin Penelitian Tanaman Rempah dan Obat Vol 22, No 1 (2011): BULETIN PENELITIAN TANAMAN REMPAH DAN OBAT
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v22n1.2011.%p

Abstract

Epidemiological studies have shown an in-verse correlation betweendiets rich in po-lyphenols, flavonoids and reduced risk of cardiovasculardisease. These associationswere mainly ascribed to the antioxidant, antiaggregation and anticholesterol capa-city of polyphenols. This association has been explained that atherogenesis is ini-tiated by hypercholesterol, lipid peroxida-tion and hyper aggregation platelet. The research was carried out to evaluate anti-oxidant, anticholesterol and antiaggrega-tion activities of methanol extract and frac-tions of black tea (Camelia sinensis). To evaluate antioxidant activity of methanol extract and fractions were compared  with (-)-Epigallocatechine 3-gallate (EGCG), an-ticholesterol activity were compared with simvastatin and antiaggregation activity were compared with aspirin. Antioxidant activity using the 1,1-diphenyl-2-picryl-hy-drazyl (DPPH) free radical scavenging acti-vity at two concentrations 100 µg/mL, 50 µg/mL and anticholesterol was cholesterol oxidase activity at two concentrations 0.005 µg/mL, 0.0025 µg/mL, the antiag-gregation  activity  used epinephrine (EPN)agonist at two concentrations 300 μM, 75 μM and one concentration 37.1 µg/mL of methanol extract and fractions of black tea. To know the difference of treatment, the data were analysed using Analysis of Variance (ANOVA) and be continued Dun-can’s new multiple range test. The results showed that DPPH scavenging activity were high (88.59-93.556%), the anticho-lesterol showed high activity (93.663-97.434%) and the antiaggregation sho-wed low up to high activity (79.967-4.31%). Using Duncan’s new multiple ra-nge test showed that anti-oxidant activity were not different among extract and fractions of black tea,the highest anti-cholesterol activity was black tea extract 0.005 µg/mL (97.434%) and the highest antiaggregation activity was ethyl acetate fraction on 75 μM EPN agonist (4.310 %), water fraction 37.1 µg/mL with 300 μM EPN agonist (21.833%).
Co-Authors Hana Ratnawati Hanna Sari Widya Kusuma Meilinah Hidayat Rizal Rizal Rr. Anisa Siwianti Handayani Sijani Prahastuti Stella Tinia Hasiana Tati Herlina Tjandrawati Mozef Victor Immanuel Wahyu Setia Widodo