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PURIFICATION AND CHARACTERIZATION OF Aeromonas media KLU 11.16 CHITOSANASE ISOLATED FROM SHRIMP WASTE Ekowati Chasanah; Gintung Patantis; Dewi Seswita Zilda; Mahrus Ali; Yenny Risjani
JOURNAL OF COASTAL DEVELOPMENT Vol 15, No 1 (2011): Volume 15, Number 1, Year 2011
Publisher : JOURNAL OF COASTAL DEVELOPMENT

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (612.616 KB)

Abstract

Our previous study found that KLU 11.16, isolated from shrimp waste secreted chitinolytic enzymes. The crude enzyme was interesting since their chitooligosccharide was able to inhibit some pathogenic bacteria. In this study we report a purification and characterization of the chitosanase enzyme produced and the identification of the KLU 11.16. Purification of the enzyme was done two steps by ion exchange chromatography followed by gel filtration. Two out of 4 peaks from Gel Filtration step, i.e. fraction 16 and 33 were capable of hydrolyzing 100% deacetylated chitosan, indicating that both fractions contained chitosanase enzyme. The enzyme from fraction 16 had approximate molecular weight of 98.3 kDa. The enzyme worked optimally at temperature of 300C, and pH 6. Addition of Ca2+, Fe2+, K+, Na+ ions in the form of Cl2 salt and detergent Triton X-100 increased the enzyme activity, while Co2+, Mn2+ and Zn2+ ions in the same concentration decreased the enzyme acitivity. Addition of EDTA and SDS significantly decreased the enzyme activity. Molecular based identification revealed that KLU 11.16 was 99% similar to Aeromonas media.
Analysis of Water Quality Status in Porong River, Sidoarjo by Using NSF-WQI Index (Nasional Sanitation Foundation – Water Quality Index) Muhammad Triaji; Yenny Risjani; Mohammad Mahmudi
Indonesian Journal of Environment and Sustainable Development Vol 8, No 2 (2017)
Publisher : Graduate Program

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (809.309 KB) | DOI: 10.21776/ub.jpal.2017.008.02.10

Abstract

Porong River can be described as a walking landfill that can deliver variety of waste to go to estuary, where it would be settled and accumulated. That numerous waste in the river will lead to pollution and provide huge negative impact for water quality status and organism life. Therefore, this study aim to assess the extent of contamination that occurred in Porong river by using WQI NSF (National Sanitation Foundation - Water Quality Index) Index. The research site was in along Porong river from Mojokerto to Jaban Sidoarjo estuary, which will be divided into 7 sampling sites. Next, the determination of the sampling location was conducted by using purposive sampling method. The use of WQI NSF index is able to analyze data using 8 or 9 parameters. In order to obtain complete overview of water quality status in Porong River, then this research used 9 parameters, including: BOD, DO, nitrate, total phosphate, temperature, turbidity, total solids, pH, and  Fecal Coliform. Based on the results of 9 parameters data analysis using NSF-WQI index, it is known that the recent water quality status in Porong River is classified as Medium criteria. By knowing the results of data analysis showing the criteria of medium, so we are obliged to maintain the water cleanness, so that the water quality status will not decline.Keywords: NSF-WQI, Porong River, Status of Water Quality
Analysis of Heavy Metal Pollutant in Wangi River Pasuruan and Its Impact on Gambusia affinis Mohammad Awaludin Adam; Maftuch Maftuch; Yuni Kilawati; Siti Nur Tahirah; Yenny Risjani
Indonesian Journal of Environment and Sustainable Development Vol 9, No 2 (2018)
Publisher : Graduate Program

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpal.2018.009.02.09

Abstract

Pollution that occurred in Pasuruan area’s watershed Wangi–Beujeng river, District of Beji, Indonesia has been initiated in 2007 and continues to this day. The activity was caused by many factors such as the industrial (I), household wastes (II) and agriculture wastes (III) as well as the erosion process.The aims of this study to analyzed the heavy metal pollution that occurs in the Wangi river flow and the effect of gill histology and antioxidant activity on gambusia fish. The research method of observation at river flow and sampling for laboratory test.Three sites were assigned for chemical sampling and tissue histologycal in this study. Fish (Gambusia affinis, local name: Gatul) and water were used as indikatorfrom each site to determine of cadmium (Cd), lead (Pb) and mercury (Hg) concentration using Atomic Absorption Spectrophotometry (AAS). The study was indicated that the streams previously used by residents for daily activities. Results showed level of cadmium (Cd), lead (Pb) and mercury (Hg) in Gambusia affinis exceeded the permissible standard (0.01 ppm Cd;0.03 ppm Pb;0.001 ppm Hg)respectively. Based on histologycal, the tissue showed of damage ofchloride cell (CC) which was used in ion homeostasis process and heavy metal route in grill fish. While protease activities, CAT, MDA and peroxidasse was increased in each sampling area, with significant different (>0.95) between the three sampling sitesKeywords: Chloride cell, Heavy metal, Toxicity, Wangi river