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All Journal JBIO: jurnal biosains (the journal of biosciences) Medical Journal of Indonesia Prosiding Seminar Biologi Elkawnie Padjadjaran Journal of Dentistry Jurnal Penelitian Kesehatan Abdimas Talenta : Jurnal Pengabdian Kepada Masyarakat Buletin Farmatera Indonesian Journal of Medicine Prosiding Seminar Nasional Biologi, Teknologi dan Kependidikan (Biotik)
Syafruddin Ilyas
Universitas Sumatera Utara
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Earth & Planetary Sciences Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

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Testosterone undecanoate and depo medroxyprogesterone acetate induced azoospermia through increased expression of spermatogenic cell caspase 3 Moeloek, Nukman; Asmarinah, Asmarinah; Siregar, Nurjati C.; Ilyas, Syafruddin
Medical Journal of Indonesia Vol 17, No 3 (2008): July-September
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (615.264 KB) | DOI: 10.13181/mji.v17i3.313

Abstract

The administration of a combination of testosterone undecanoate (TU, a long-acting androgen) and depo-medroxyprogesterone acetate (DMPA) were investigated in term of suppression of rat sperm concentration in vivo to azoospermia through increasing activity of spermatogenic cell caspase 3. Adult Sprague Dawley rats received TU and DMPA of 2.5 mg and 1.25 mg, respectively, a regimen known to rapidly reduce intra testicular testosterone and to produce azoospermia within 12 weeks. Caspase 3 positive sperm cells increased compared with control levels during 6 weeks post-injection and increased further through 60 weeks. Immunohistochemistry for caspase 3 revealed that spermatocytes represented the predominant caspase 3 positive germ cells. Modest immunoreactivity for caspase-3 was localized to nuclear region of the germ cells of control and treated testes. Immunohistochemistry study revealed significantly increased caspase-3 expression in nuclei of germ cells during administration of TU+DMPA to rats. Additionally, the caspase 3 content was significantly increased in germ cells during rats were administered TU+DMPA (453.90±84.88 cells/200 seminiferous tubules) and caspase 3 significant increase in immunoreactivity was localized to the nuclei of spermatogonia, spermatocytes, and spermatids. Taken together, these results indicated that azoospermia due to reduced intratesticular testosterone concentration was caspase-3 activation dependent and suggested that the increase in active caspase-3 in the nucleus may be involved in the induction of decreased sperm production. (Med J Indones 2008; 17: 149-56)Keywords: TU, DMPA, sperm concentration, germ cells
Anticancer potency of black sea cucumber (Holothuria atra) from Mentawai Islands, Indonesia Satari, Mieke Hemiawati; Arma, Utmi; Ilyas, Syafruddin; Handayani, Dian
Padjadjaran Journal of Dentistry Vol 29, No 1 (2017): March
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (148.217 KB) | DOI: 10.24198/pjd.vol29no1.11654

Abstract

Introduction: The source of bioactive compounds believed to have strong anticancer potency is derived from sea cucumber. Black sea cucumber (Holothuria atra) is a dominant species in Mentawai Islands, West Sumatera, Indonesia. Key factor compound that acts as anticancer in sea cucumber extract is tritepenoid also known as Frondoside A. The purpose of this study was to determine the effectiveness of the active compound taken from black sea cucumber as anticancer. Methods: Methods used was experimental laboratory. Anticancer activity of black sea cucumber was determined by using the MTT assay cytotoxic test to measure the IC50. Cytotoxic test was conducted with Supris clone-1 (SP-C1) derived from cloned lymphadenopathy of patients with oral squamous cell carcinoma on lingual part. Results: It was found that the compounds contained in black sea cucumber has strong anticancer activity (IC50 = 5.25 μL/mL) against SP-C1. Conclusion: Black sea cucumber extract has strong cytotoxic effects against cancer cells SP-C1, therefore black sea cucumber extract potentially developed as a new source of cancer medicine.
Effects of Bean Ethanol Extract (Phaseolus Vulgaris L.) on Superoxide Dismutase Activity in Wistar Mice (Micetus Norvegicus) Hyperglycemia Sinaga, Ade Putra Fratama; Ilyas, Syafruddin; Anggraini, Dwi Rita
Indonesian Journal of Medicine Vol 2, No 2 (2017)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

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Abstract

Background: Aloxan tetrahyd micee causes free radicals and higher reactive oxidative species (ROS) production, being able to lead to reduce insulin secretion from pancreatic β cells and receptor sensitivity in insulin-receptor cells resulting in hyperglycemia conditions. Bean extract ethanol can reduce oxidative stress. The purpose of this study was to determine bean’s ethanol extract effectiveness in lowering blood sugar levels and increasing the activity of superoxide dismutase (SOD) enzyme.Subjects and Methods: This was a randomized controlled trial (RCT) study. The samples were mice (Micetus norvegicus) of wistar strains randomized in five groups: P1-negative control, positive P2-control, P-3 ethanol extract group of 200mg / kgBW, P4-group 400mg / kgBW, P5-group 600 mg / kgBW. Dependent variables are blood sugar (mg/ dL) and superoxide dismutase enzymes. The independent variable is the bean extract of ethanol. Differences in blood sugar levels and superoxide enzyme levels of intergroup dismutase were tested by Anova test, followed by post hoc test, to determine the effectiveness of bean extract ethanol.Results: This study showed a statistically significant decrease in blood glucose levels after ethanol extract of green beans 600 mg / kg BW from day 14 to day 28 (p <0.05). This study did not show a decrease in blood sugar levels after ethanol extract of green beans 200 mg / kgBW and 400 mg / kgBW from day 7 until day 28 (p> 0.05). Giving ethanol extract of green beans did not affect the activity of superoxide dismutase enzyme (p = 0.830).Conclusion: Giving ethanol extract of green beans 600 mg / kgBW effectively decrease blood sugar in male wistar mice (Micetus norvegicus) from day 14 to day 28. Giving ethanol extract of green beans 200 mg/ kgBW, 400 mg / kgBW, and 600 mg / kgBW, is not effective to increase the activity of superoxide dismutase enzyme.Keywords: Phaseolus Vulgaris L., blood sugar level, superoxide dismutase enzymeCorrespondence: Ade Putra Fratama Sinaga. Masters Program on Biomedics, Faculty of Medicine, North Sumatra University. Email: adeputra535@ymail.com.Indonesian Journal of Medicine (2017), 2(2): 139-145https://doi.org/10.26911/theijmed.2017.02.02.08
Effect of Lawsonia inermis Linn. Ethanol Extract on the Superoxyde Dismutase Activity in Hyperglycemic Rattus norvegicus Antika, Maya Anjelir; Ilyas, Syafruddin; Sari, Mutiara Indah
Indonesian Journal of Medicine Vol 2, No 2 (2017)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

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Abstract

Background: Alloxan causes experimental diabetic conditions (hyperglycemia) in experi­mental animals through oxidation and the formation of free radicals (oxidative stress) that damage pancreatic β cells. The ethanol extract of the henna leaves can decrease oxidative stress. The purpose of this study is to know the effect of ethanol extract of henna leaves to decrease blood sugar level and increase superoksida enzyme activity.Subjects and Methods: This study is an experimental study. The study design of posttest only control group design, using mice (Rattus norvegicus) strains of wistar, was randomized in five groups: negative control, positive control, group obtaining ethanol extract of henna leaves 200 mg/kg BW, 400 mg/kg BW and 600 mg/kg BW.Results: This study showed a significant reduction in blood glucose levels when compared to the control group. Superoxide dismutase enzyme activity increased but was not statistically significant.Conclusion: Ethanol extract of henna leaves 400 mg/kg BW significantly decreased mice blood glucose level, there was no significant difference in the increase of superoxide dis­mutase enzyme activity.Keywords: Alloxan, antioxidant, flavonoids, hyperglycemia, oxidative stress.Correspondence: Maya Anjelir Antika. Masters Program of Biomedic, Faculty of Medicine, University of North Sumatera.Indonesian Journal of Medicine (2017), 2(2): 79-85https://doi.org/10.26911/theijmed.2017.02.02.01
Pengaruh Pemberian EVOO Terhadap Berat Badan Lahir Tikus Putih (Rattus norvegicus) pada Induk Model Preeklampsia Evi Irianti; Syafruddin Ilyas; Rosidah Rosidah; Salomo Hutahaen; Ramlan Silaban
Jurnal Penelitian Kesehatan SUARA FORIKES Vol 11 (2020): Nomor Khusus Mei-Juni 2020
Publisher : FORIKES

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/sf11nk314

Abstract

Pre eclampsia is believed to be caused by a missmatch of antioxidants in the body which can be prevented by the administration of EVOO. EVOO which is proved to be rich in tocopherol which plays an important role in preventing this missmatch, thus both free radicals and antioxidants are in balanced concentration, particularly during pregnancy. This study was a laboratory based pre and post test with control group design which was conducted in total of 25 pregnant female albino rats from Sprague Dawley strain with body weight of approximately 200 grams. Samples were then grouped into P0 as control group and other 4 experimental group (P1, P2, P3, P4). Experimental group was given injection of 6% sodium chloride solution as much as 3 ml/day on the 6th until 12th day of their pregnancy and was given stimulation of acute stress on day 18th to model these groups into having pre eclampsia. All these four groups were then given EVOO on day 13th to 19th, except P1. On day 20th, all samples were executed. We found that birth weight among rats within experimental groups which were given either mild or moderate dose of EVOO showed no significant difference compared to control group, 71,43% fetus was found dead in P1, and 41,94% was found in P4. Thus, we concluded that EVOO was able to suppress free radicals and we suggested that other natural antioxidants, such as GPx, GSH and vitamin E should be furtherly investigated to see how these antioxidants work in altering the lipid peroxidation process after the administration of EVOO. Keywords: EVOO; birth weight ABSTRAK Salah satu penyebab preeklampsia adalah ketidak seimbangan antioksidan di dalam tubuh dan dapat dicegah dengan pemberian EVOO. Pemberian EVOO yang kaya akan kandungan tokoferol bertujuan untuk mencegah ketidakseimbangan antara produksi radikal bebas dan antioksidan khususnya pada kondisi hamil. Rancangan penelitian ini pre and post test with control goup design di laboratorium. Subyek tikus putih betina bunting, galur Sprague Dawley, BB ±200g. Total sampel 25 ekor terdiri atas kelompok kontrol (P0) dan 4 kelompok perlakuan sebagai model preeklampsia (P1, P2, P3, P4). Model preeklampsia dilakukan dengan injeksi NaCl 6% 3 ml/hari pada hari ke 6 – 12 periode kebuntingan dan stres akut hari ke18 sekali saja. Seluruh kelompok perlakuan diberi EVOO kecuali P1, dari hari ke 13 - 19. Hari ke 20, seluruh tikus dieksekusi. BBL kelompok perlakuan yang diberi EVOO dosis rendah maupun sedang tidak beda dengan kontrol, 71,43% fetus mati pada kelompok P1, dan 41,94% pada P4. Kesimpulan EVOO mampu menekan radikal bebas dalam darah. Disarankan untuk mengukur antioksidan alami lainnya seperti GPx, GSH serta vitamin E untuk mengetahui peranan dalam mereduksi peroksida lemak setelah pemberian EVOO. Kata kunci: EVOO; berat badan lahir
Pengaruh Ekstrak Methanol Biji Pare (Momordica charantia) dan DMPA Terhadap Jumlah Sel purkinje Cerebellum Mencit (Mus musculus L.) SYAFRUDDIN ILYAS; SALOMO HUTAHAEAN; NURSAL NURSAL
Prosiding Seminar Biologi Vol 2 No 1 (2016): Prosiding Seminar Nasional From Basic Science to Comprehensive Education
Publisher : Jurusan Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/psb.v2i1.3379

Abstract

Perubahan jumlah sel purkinje cerebellum mencit dapat dijadikan sebagai indikator terjadinya gangguan terhadap otak kecil pada pemberian ekstrak methanol biji pare (Momordica carantia L.) dan DMPA. Metode eksperimen digunakan untuk menentukan perbedaan yang terjadi pada tiap kelompok kontrol dan pemberian lama pemberian ekstrak biji pare dan DMPA. Kelompok kontrol terbagi dalam K0, K1, dan K2 dengan waktu berturut-turut 0, 4, dan 8 minggu. Kelompok perlakuan terdiri dari (P0) biji pare 0 minggu secara oral dan DMPA intramuskular (@ 6 jam), (P1) biji pare dan DMPA (@4 minggu), (P2) biji pare dan DMPA (@8 minggu). Masing-masing kelompok terdiri dari 5 mencit sehingga total mencit jantan adalah 30 ekor. Dosis ekstrak metanol biji pare adalah 5mg/10g berat badan mencit yang diberikan secara oral) (Yama et al. 2011). Sedangkan dosis DMPA sebesar 0,175 mg/ekor mencit yang diberikan secara intramuskular (Ilyas 2014). Hasil penelitian menunjukkan tidak adanya perbedaan yang nyata  (p>0,05) antara kontrol dan perlakuan pada 0, 4, dan 8 minggu terhadap jumlah sel purkinje cerebellum mencit. Disimpulkan bahwa pemberian ekstrak metanol biji pare dan DMPA aman terhadap perbedaan jumlah cerebellum mencit. Kata kunci: Sel purkinje, Momordica charantia L., DMPA, Mus musculus L. 
HUBUNGAN ANTARA KUALITAS AIR DENGAN KEBIASAAN MAKANAN IKAN BATAK (Tor soro) DI PERAIRAN SUNGAI ASAHAN SUMATERA UTARA Budianto Siregar; Ternala Alexander Barus; Syafruddin Ilyas
JBIO: jurnal biosains (the journal of biosciences) Vol 1, No 2 (2013): Bio
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v1i2.12508

Abstract

Telah dilakukan penelitian tentang hubungan antara kualitas air dengan kebiasaan makanan ikan batak (Tor soro) di perairan Sungai Asahan Sumatera Utara pada bulan November 2012-Januari 2013. Tujuan penelitian ini adalah untuk mengetahui kebiasaan makanan ikan batak (Tor soro), kualitas air Sungai Asahan, serta hubungan antara kualitas air dengan kebiasaan makanan ikan batak (Tor soro). Pengambilan sampel dilakukan dengan menggunakan electrofishing dan jala serta isi lambung dianalisis dengan menggunakan metode volumetrik. Hasil penelitian menunjukkan bahwa ikan batak (Tor soro)  memiliki pola pertumbuhan alometrik. Makanan utama ikan batak (Tor soro) berdasarkan Index of Preponderance: Cladophora (47,01%, 62,29%, 71,11%) pada stasiun 1, 4, dan 5. Cymbella (47,57%) pada stasiun 2, Vaucheria (51,46%) pada stasiun 3. Temperatur, Intensitas cahaya, kecerahan dan pH berkorelasi kuat terhadap Cladophora. Nitrat dan posfat berkorelasi kuat terhadap Cymbella. Nitrat berkorelasi kuat terhadap Vaucheria. Kondisi Sungai Asahan secara relatif dalam keadaan baik dan mendukung pertumbuhan ikan batak (Tor soro).
HUBUNGAN ANTARA KUALITAS AIR DENGAN KEBIASAAN MAKANAN IKAN BATAK (Tor douronensis) DI PERAIRAN SUNGAI ASAHAN SUMATERA UTARA Ria Lumbantoruan; Ternala Alexander Barus; Syafruddin Ilyas
JBIO: jurnal biosains (the journal of biosciences) Vol 1, No 2 (2013): Bio
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v1i2.12728

Abstract

Telah dilakukan penelitian tentang hubungan antara kualitas air dengan kebiasaan makanan ikan batak (Tor douronensis) di Perairan Sungai Asahan Sumatera Utara. Tujuan penelitian ini adalah untuk mengetahui kualitas air Sungai Asahan, kebiasaan makanan ikan batak (Tor douronensis) serta hubungan antara kualitas air dengan kebiasaan makanan ikan batak (Tor douronensis). Hasil penelitian menunjukkan bahwa ikan batak memiliki pola pertumbuhan alometrik. Makanan utama ikan batak berdasarkan indeks prepoderannya adalah Cymbella (56,32%), Navicula (46,65%), Thiothrix (44,25%), dan Ulothrix (76,21%). Temperatur berkorelasi kuat terhadap Thiothrix. Kecerahan berkorelasi kuat terhadap Thiothrix. Intensitas Cahaya berkorelasi sangat kuat terhadap Navicula. Arus berkorelasi kuat terhadap Navicula. Parameter pH berkorelasi sangat kuat terhadap Cymbella. DO berkorelasi kuat terhadap Navicula. BOD 5 berkorelasi kuat terhadap Navicula. NO3 berkorelasi kuat terhadap Thiothrix. PO4 berkorelasi berkorelasi kuat terhadap Thiothrix. Kondisi Sungai Asahan mengindikasikan secara relatif dalam keadaan baik dan mendukung pertumbuhan ikan batak (Tor douronensis).
HUBUNGAN ANTARA KUALITAS AIR DENGAN KEBIASAAN MAKANAN IKAN BATAK (Neolissochilus sumatranus) DI SUNGAI ASAHAN, SUMATERA UTARA Mesrawati Purba; Ternala Alexander Barus; Syafruddin Ilyas
JBIO: jurnal biosains (the journal of biosciences) Vol 1, No 2 (2013): Bio
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v1i2.12729

Abstract

The study of the water quality relationship and food habits of batak fish (Neolissochilus sumatranus) in Asahan river, North Sumatra was conducted. Aims of this research are to undesrtand the food habits of batak fish , the water quality of Asahan river and relationship between water quality and food habits of batak fish. Fish samples were caught by electrofishing and casnet and stomach content was analysis using volumetric method. Results showed that the batak fish  have allometric growth patten. Based on the data of stomach content shown that 38 organism, the major food of batak fish  based on index preponderance are Cymbella (42,56%), Navicula (56,90%) and Nitschia (47,09 %). Cymbella positively correlated with light penetration, light intensity, DO and BOD5 and negatively correlated with temperature,water current, pH, NO3 and PO4. Navicula positively correlated with water current, pH, DO, BOD5, NO3 and PO4 and negatively correlated with temperature, light penetration and light intensity. Nitzschia positively correlated with light intensity,   temperature, light penetration, pH and PO4 and negatively correlated with water current, DO, BOD5 and NO3. Indicating the condition of Asahan river is relatively in good condition and support fish growth as well
IKAN BATAK (Neolissochillus sumatranus) SEBAGAI BIOINDIKATOR PENCEMARAN LOGAM BERAT TIMBAL (Pb) DAN CADMIUM (Cd) DI PERAIRAN SUNGAI ASAHAN SUMATERA UTARA Rina Marintan Sitorus; Ternala Alexander Barus; Syafruddin Ilyas
JBIO: jurnal biosains (the journal of biosciences) Vol 1, No 2 (2013): Bio
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v1i2.12735

Abstract

Penelitian ikan batak (Neolissochillus sumatranus) sebagai bioindikator pencemaran logam berat Pb dan Cd di sungai Asahan. Pengambilan sampel dilakukan pada bulan  Januari 2013 di 5 stasiun pengamatan. Sampel yang diambil adalah ikan dan air. Parameter yang diamati adalah logam berat (Pb dan Cd), kualitas air (temperatur, arus, kecerahan dan intensitas cahaya) dan unsur hara (nitrat dan posfat). Kandungan logam berat Pb  di air (<0,01 mg/L) dan pada ikan (<0,054 mg/L)  masih di diluar batas baca untuk deteksi limit. Kandungan Cd di air berkisar antara 0,002-0,007 mg/L, nilai ini masih dibawah baku mutu air golongan I (Peraturan Pemerintah No. 82 tahun 2001), dan di air nilai Cd diluar batas baca untuk deteksi limit yaitu <0,003 mg/L. Konsentrasi logam (Pb dan Cd) di air dan ikan masih memenuhi standar baku mutu. Klasifikasi mutu air kelas IIL dengan metode storet disimpulkan bahwa setiap stasiun tergolong kualitas air kelas A kategori perairan baik sekali.  
Co-Authors Anggraini, Dwi Rita Antika, Maya Anjelir Asmarinah Asmarinah Budianto Siregar Datten Bangun Dian Handayani Dian Handayani Evi Irianti Fatchiyah Fatchiyah Fauzi Yusuf, Fauzi Halimatussakdiah Halimatussakdiah Harry Agusnar Harun Al Rasyid Damanik, Harun Al Rasyid Kesuma Wardani Masitta Tanjung Mesrawati Purba Mieke Hemiawati Satari Mieke Hemiawati Satari Nukman Moeloek Nurjati C. Siregar Nursal Okto P.E Marpaung Ramlan Silaban Ria Lumbantoruan Rina Marintan Sitorus Rosidah Rosidah Salomo Hutahaean Salomo Hutahaen Sari, Mutiara Indah Silvia W Lestari Sinaga, Ade Putra Fratama Siregar C Siregar, Siregar C Ternala Alexander Barus Tisna Harmawan Tisna Hermawan Utmi Arma Utmi Arma, Utmi Vivi Mardina Vivi Mardina Yasmeini Yazir