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Pemanfaatan Bakteri Sebagai Pereduksi Emisi Gas Metana Pada Limbah Cair Kelapa Sawit (Elaeis guineesis Jacq.) Nisa Widya Amanda; I Made Artika; Iman Rusmana
Current Biochemistry Vol. 4 No. 2 (2017)
Publisher : IPB University

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Abstract

Kelapa sawit merupakan salah satu tumbuhan penghasil minyak terbesar di Indonesia. Proses pengolahan buah kelapa sawit menjadi minyak memiliki dampak negatif, yakni terciptanya gas metana. Gas metana merupakan salah satu gas penyumbang efek rumah kaca yang menyebabkan pemanasan global. Oleh karena itu, diperlukan cara untuk menurunkan emisi gas metana. Tujuan penelitian ini adalah penggunaan bakteri untuk menurunkan emisi gas metana pada limbah cair pemrosesan buah kelapa sawit. Bakteri diisolasi dari limbah cair kelapa sawit. Terhadap isolat tunggal yang diperoleh dilakukan uji aktivitas enzim soluble methane monooxygenase (sMMO) dan particulate methane monooxygenase (pMMO). Isolat dengan aktivitas methane monooxygenase tertinggi dipilih untuk analisis sekuen DNA dan konstruksi pohon filogenetika. Tahap isolasi bakteri menghasilkan 13 isolat koloni tunggal. Uji aktivitas methane monooxygenase menunjukkan bahwa semua isolat tidak memiliki aktivitas sMMO. Sebaliknya, semua isolat memiliki aktivitas pMMO dengan tingkat berbeda yaitu dengan rentang nilai antara 0.10 – 0.22 M/mL kultur/hari. Analisis sekuen DNA menunjukkan bahwa isolat terpilih adalah Klebsiella sp. Berdasarkan uji Gram diketahui bahwa bakteri ini termasuk bakteri Gram negatif. Bakteri berpotensi digunakan untuk menurunkan emisi gas metan pada limbah cair pemrosesan buah kelapa sawit. Kata kunci: Klebsiella sp, 16S rRNA, Metana, pMMO, sMMO.
The Abundance of Nitrogen Fixing, Nitrifying, Denitrifying and Ammonifying Bacteria in the Soil of Tropical Rainforests and Oil Palm Plantations in Jambi Zulfarina Zulfarina; Iman Rusmana; Nisa Rachmania Mubarik; Dwi Andreas Santosa
Makara Journal of Science Vol 21, No 4 (2017): December
Publisher : Directorate of Research and Community Engagement, Universitas Indonesia

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Abstract

Alterations in the use of land for oil palm plantations can change the domination and activity of soil bacteria. More specifically, alteration in soil microbial communities can directly affect soil ecosystem functioning, particularly with respect to carbon and nitrogen cycles. Nitrogen can be a limiting nutrient, and the availability of nitrogen in the soil environment becomes a major factor in controlling the production of biomass. This research project aimed at studying the abundance of nitrogen-fixing, nitrogen-oxidizing, nitrogen-reducing, and ammonifying bacteria based on their functional genes in the tropical rain forests of Taman Nasional Bukit Duabelas (TNBD) and the oil palm plantations in Sarolangun Jambi. Samples were collected in November 2015. Soil sampling was performed randomly at three points representing each area of ​​the tropical rainforests of TNBD and the seven- to eight-year-old oil palm plantations. Soil samples were collected using a soil sample core from 0–15 cm below the surface with depth strata of 0–5 cm, 5–10 cm, and 10–15 cm. Composite assessment was conducted on samples from each point corresponding to each respective depth strata. Soil samples were stored at -20ºC prior to testing. Microbial abundance was measured using the most probable number (MPN) method. The abundance of microbes that play a role in nitrogen metabolism between strata of 5–10 cm and 10–15 cm does not appear to be different. The highest abundance of microbes in oil palm plantation land in Jambi was found in samples with nitrifying bacteria, later followed by denitrifying, nitrogen-fixing, and ammonifying bacteria. Ultimately, it was found that microbial abundance in oil palm plantations was higher than the corresponding rates in samples from tropical rainforests.
Salmonella population in waste water treatment installation (IPAK) at Pulo Gebang DKI Jakarta Rusmana, Iman; Budiarti, Sri
Medical Journal of Indonesia Vol 7 (1998): Supplement 1
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (82.759 KB) | DOI: 10.13181/mji.v7iSupp1.1124

Abstract

[no abstract available]
Penapisan dan Identifikasi Bakteri Kitinolitik Penghambat Pertumbuhan Ganoderma boninense in Vitro Risky Hadi Wibowo; Nisa Rachmania Mubarik; Iman Rusmana; Maggy Thenawidjaya
Jurnal Fitopatologi Indonesia Vol. 13 No. 3 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (383.406 KB) | DOI: 10.14692/jfi.13.3.105

Abstract

Chitinolytic bacteria have been reported as biocontrol agents and have the ability to produce chitinase enzymes. The objective of the research was to obtain chitinase producing bacteria that had antagonistic activity to Ganoderma boninense, a causal agent of basal stem rot on oil palm. A total of 63 isolates of chitinase producing bacteria were isolated from soil of Bukit Dua Belas National Park and oil palm plantation in Jambi Province; all was screened for their potency in inhibiting G. boninense in vitro. Three isolates designated TB04-05, SW01-11, and SW02-08 were potentially suppressed and inhibited the mycelium growth of G. boninense in vitro. Based on their specific chitinase activity, these three isolates produced the highest level of chitinase enzyme of 6.3072 U mg-1 protein, 6.0385 U mg-1 protein and 6.1279 U mg-1 protein, respectively after 24 hr incubation. Based on 16S RNA identification, strain TB04-05 had similarity with Bacillus cereus, whereas strains SW01 and SW02-08 had similarity with Bacillus thuringiensis.
Physiological Characterization and Molecular Identification of Denitrifying Bacteria Possesing Nitrous Oxide High Reduction Activity Isolated from Rice Soils RATNA SETYANINGSIH; IMAN RUSMANA; PRIHASTO SETYANTO; ANTONIUS SUWANTO
Microbiology Indonesia Vol. 4 No. 2 (2010): August 2010
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5454/mi.4.2.5

Abstract

Rice fields are one of the main sources of high nitrous oxide N2O emission. Soil denitrifiers possessing high N2O reduction activity are important for controlling N2O emission. Nitrous oxide reduction is the last step of denitrification process. The aims of this study were to characterize and to identify denitrifying bacteria isolated from rice soils possessing high activity of N2O reduction. Soil samples were taken from 6 locations of rice fields in Bogor (West Java) and Tangerang (Banten), Indonesia. Physiological characterization was performed using API 20 NE, while molecular identification was conducted based on the 16S rRNA gene sequence. It was found that ten isolates of denitrifying bacteria were able to grow using N2O as an electron acceptor as indicated by decreasing N2O concentration in the headspace of the cultures. The bacterial growth indicated by optical density, increased up to 0.12-0.47 after 5 days incubation. Isolate BL2 had the highest activity of N2O reduction followed by BL1 and BLN1 at up to 5.41, 4.09, and 3.91 μmol mL-1 bacterial cultures, respectively. The BL1, BL2, and BLN1 isolates had some different physiological characteristics. Based on their 16S rRNA sequence, BL1 and BLN1 were closely related to Ochrobactrum anthropi ATCC 49188 with similarity of 99%.
Potensi Quorum Quencher Bakteri Filosfer dan Rizosfer terhadap Dickeya dadantii Taruna D. Satwika; Iman Rusmana; Alina Akhdiya
Jurnal AgroBiogen Vol 13, No 2 (2017): Desember
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v13n2.2017.p101-110

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Ekspresi gen-gen virulensi pada Dickeya dadantii diatur oleh proses quorum sensing menggunakan asil-homoserin lakton (AHL) sebagai molekul sinyal. Patogenisitas bakteri tersebut dapat dihambat oleh aktivitas quorum quenching (QQ) bakteribakteri penghasil AHL-laktonase. Tujuan penelitian ini adalah mengisolasi dan mengarakterisasi bakteri penghasil AHLlaktonase asal rizosfer dan filosfer yang berpotensi untuk dikembangkan sebagai quorum quencher untuk D. dadantii. Isolasi bakteri dilakukan dari sampel daun dan sampel tanah rizosfer beberapa komoditas tanaman asal Sukabumi, Tegal, Kupang, dan Wonosobo. Sebanyak 8 dari 79 isolat bakteri yang diperoleh menunjukkan aktivitas QQ terhadap bioindikator Chromobacterium violaceum. Bioasai respons hipersensitif (hypersensitive response) yang dilakukan pada tanaman tembakaumenunjukkan enam (KT2, KT9, KT10, KUT1, TKF2, and WKF3) dari delapan isolat tersebut tidak menimbulkan respons hipersensitif. Keenam isolat tersebut mampu menekan virulensi D. dadantii pada umbi kentang. Sekuen 16S rRNA enam isolat tersebut memiliki kemiripan tertinggi dengan Bacillus cereus, B. aryabhattai, B. acidiceler, dan Micrococcus aloeverae. B. cereus KT9 and B. aryabhattai TKF2 terdeteksi memiliki gen penyandi AHL-laktonase (aiiA). Ini merupakan laporan yang pertama tentang aktivitas QQ pada spesies M. aloeverae, B. aryabhattai, and B. acidiceler. Keberadaan gen aiiA pada B.aryabhattai juga belum pernah dilaporkan sebelumnya. Penelitian ini memberikan informasi baru tentang aktivitas QQ ketiga isolat tersebut dan potensinya sebagai quorum quencher untuk D. dadantii.
Bacterial and Fungal Communities in Tempeh as Reveal by Amplified Ribosomal Intergenic Sequence Analysis CECILIA ANNA SEUMAHU; ANTONIUS SUWANTO; IMAN RUSMANA; DEDY DURYADI SOLIHIN
HAYATI Journal of Biosciences Vol. 20 No. 2 (2013): June 2013
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (142.073 KB) | DOI: 10.4308/hjb.20.2.65

Abstract

Tempeh is an Indonesian traditional fermented food produced using Rhizopus as a starter culture. In practice, however, the starter culture as well as fermentation processes would yield a polymicrobial fermentation, which generated a unique tempeh flavor and texture. This condition makes Indonesian tempeh as one of the most complex fermented food, while at the same time would make it difficult to scale up tempeh production with uniform quality and consistency. The aim of this study was to compare a number of tempeh microbial communities employing Amplified Ribosomal Intergenic Sequence Analysis (ARISA). Fresh tempeh samples were obtained from tempeh producers in Java and Moluccas. 16S rRNA gene libraries and DNA sequencing were employed to analyze further the nature of bacterial diversity in two selected tempeh samples. The results of our study showed that different tempeh producer possessed different Bacterial ARISA (BARISA) or fungi ARISA (FARISA) profiles.  However, BARISA profiles were found to be more discriminative than FARISA, and therefore BARISA would be more useful for tempeh genetic fingerprint or barcoding.
Fluks Bentik dan Potensi Aktivitas Bakteri Terkait Siklus Nitrogen di Sedimen Perairan Mangrove Pulau Dua, Banten Iswantari, Aliati; Wardiatno, Yusli; Pratiwi, Niken T.M; Rusmana, Iman
JURNAL BIOLOGI INDONESIA Vol 10, No 1 (2014): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (265.728 KB) | DOI: 10.14203/jbi.v10i1.336

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Mangrove ecosystem has important role as source of nutrient particularly nitrogen in coastal area. Nitrogen (N) is alimiting factor in marine and coastal area. The aim of this research was to study benthic fluxes and potency ofbacterial activity in sediment of mangroves area, related to nitrogen cycle. This research was conducted in floodedmangroves area in Pulau Dua. The sediment and overlying water was sampled using sediment core sampler.Experimental treatment for flux analysis and sediment-slurry were conducted in three hours. Nutrient of NH3-N,NO2-N, and NO3-N and abundance of nitrifier, denitrifier, DNRA, and ammonifier were analyzed. The resultsshowed that the abundance of anaerobic bacteria was higher than aerobic bacteria. The dominance of bacterialgroups found in sediment was ammonification bacteria. The highest nutrient concentration in sediment was NH3-N. Benthic fluxes value showed higher NH3-N tends to release from the sediment to water than NO2-N and NO3-N. Generally, mangrove sediment in Pulau Dua has higher potency ofbacterial activity (Vmax and Km) in NO3reduction by anaerobic bacteria than NH3 oxidation by aerobic bacteria.Keywords: bacteria, benthic fluxes, mangrove, potency of bacterial activity, sediment
Identification of nifD and nifH Genes of Methanotrophic Bacteria from Rice Field Bintarti, Ari Fina; Rusmana, Iman; Wahyudi, Aris Tri
ANNALES BOGORIENSES Vol 18, No 2 (2014): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (879.425 KB) | DOI: 10.1234/97

Abstract

Metanotrophic bacteria have ability to oxidize methane and fix atmospheric nitrogen, hence the bacteria has an important role as a nitrogen source provider on wetland area like rice fields. Nitrogen fixation process is catalyzed by the nitrogenase enzyme complex, encoded by nifD and nifH genes. However, characteristic of these genes from indigenous-methanotrophic bacteria still poorly understood. Hence, nifD and nifH genes of methanotrophic bacteria isolated from rice fields in Indonesia (BGM3, BGM9, SS1, SS3, SS10, ST18, SP3 and INP4) were identified and characterized. Detection of nifH and nifD genes was conducted by polymerase chain reaction (PCR) amplification. nifH and nifD gene sequences were analyzed using BLAST-X and phylogenetic trees were constructed using Neighbour Joining method. Based on nifH sequences analysis, SS1 closely related to Beijerinckia mobilis and SS3, SS10, ST 18 closely related to Beijerinckia indica subsp. indica ATCC 9039, while, BGM3, INP4, and BGM9 related to nifH of uncultured nitrogen-fixing bacterium. In other hand, sequence analysis of nifD gene showed that SS1, SS3, SS10, ST 18 closely related to B. indica subsp. indica ATCC 9039 and BGM3, BGM9, INP4 closely related to Xanthobacter autotrophicus Py2. Identification by 16 SrRNA indicated that SS1, SS3, SS10, and ST18 had closeness to Beijerinckia sp. P310-1, while INP4 closely related to Xanthobacter sp. M5C24.
STRUKTUR KOMUNITAS BAKTERI PENGOKSIDASI AMONIA BERDASARKAN GEN amoA DI SITU SAWANGAN-BOJONGSARI, JAWA BARAT Novita, Lena; Rusmana, Iman; Widiyanto, Tri
LIMNOTEK - Perairan Darat Tropis di Indonesia Vol 21, No 1 (2014)
Publisher : Research Center for Limnology

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Abstract

Salah satu permasalahan dalam pengelolaan perairan darat adalah penurunan kualitas air yang disebabkan oleh polusi senyawa nitrogen. Mekanisme transformasi senyawa nitrogen oleh bakteri indigenous menjadi langkah penting untuk mengatasi permasalahan tersebut. Aktivitas antropogenik di sekitar Situ Sawangan-Bojongsari memungkinkan terjadinya polusi senyawa nitrogen seperti amonia. Penelitian bertujuan untuk mengetahui struktur komunitas bakteri pengoksidasi amonia serta faktor fisika dan kimia yang mempengaruhinya di Situ Sawangan-Bojongsari. Kelimpahan dan keragaman bakteri pengoksidasi amonia dipengaruhi oleh faktor fisika dan kimia perairan. Kelimpahan tertinggi bakteri pengoksidasi amonia terdapat pada strata 0 cm (270 sel/mL) dan kelimpahan terendah terdapat pada strata 230 cm (73 sel/mL). Bakteri pengoksidasi amonia tidak ditemukan pada bagian sedimen. Gen amoA hanya teramplifikasi dari contoh air. Sebanyak 10 pita gen amoA dengan posisi yang berbeda dapat terdeteksi pada gel DGGE. Sebanyak enam isolat gen amoA memiliki kemiripan sekuen nukleotida dengan amo A dari uncultured bacterium (86-97%). Sekuen asam amino dari keenam isolat gen amo A menunjukkan kemiripan dengan protein ammonia monooxygenase (56-93%). Sebanyak lima isolat gen amo A teridentifikasi sebagai ammonia monooxygenase dari uncultured bacterium dan satu isolat sebagai ammonia monooxygenase dari Nitrosospira sp. III7. Berdasarkan analisis filogenetik, keenam isolat gen amoA termasuk ke dalam genus Nitrosospira.
Co-Authors . Mahyarudin Achmad Dinoto Aliati Iswantari ALINA AKHADIYA Alina Akhdiya Alina Akhdiya ALINA AKHDIYA Amanda, Nisa Widya Andri Ferbiyanto Angela Mariana Lusiastuti Angela Mariana Lusiastuti Ani Suryani Ani Widiyati ANIK KUSMIATUN Anja Meryandini Anja Meryandini Antonius Suwanto dan Meity S. Sinaga . Budi Tjahjono Andi Khaeruni R Ari Fina Bintarti Ari Fina Bintarti, Ari Fina Aris Tri Wahyudi Baskoro S Wibowo Cahyadi Irwan Cahyadi, Alfan Cahyadi, Alfan Cecep Kusmana CECILIA ANNA SEUMAHU Ciptadi Achmad YUSUP Daniel Djokosetiyanto Darmono Taniwiryono Desniar . Dewi Puspaningsih DIAH ISKANDRIATI Dian Hendrayanti Dinamella Wahjuningrum Dondin Sajuthi DWI ANDREAS SANTOSA Dwi Ningsih Susilowati Eddy Supriyono Emei Widiyastuti Fachriyan Hasmi Pasaribu Fachriyan Hasmi Pasaribu Faturrahman Faturrahman Fredinan Yulianda GALUH WENING PERMATASARI Hamim Hamim Happy Widiastuti Hefni Effendi Hessy Novita Hessy Novita I Komang Gede Wiryawan I MADE ARTIKA I Made Samudra Ifa Manzila It Jamilah KATHARINA JESSICA Khairul Syahputra Kukuh Nirmala Lena Novita Lizawati . Maggy Thenawidjaya Maggy Thenawidjaya Suhartono Maggy Thenawidjaya Suhartono Mahdani, Wilda Masrukhin Masrukhin Masthura, Aderiana Miftahudin . Mukti Ali Munti Yuhana MUTTAQIN, MAFRIKHUL Niken T.M Pratiwi, Niken T.M NISA RACHMANIA MUBARIK Nisa Widya Amanda Novita, Lena PRIHASTO SETYANTO Prihasto Setyanto Priyo Handoyo Wicaksono Rahman Rahman Rahman Rahman Rahman Rahman Rahman Rahman Rahman Rahman Rahminovita Herlis RATNA SETYANINGSIH RATNA SETYANINGSIH Ridwan Affandi RIKA RAFFIUDIN Rina Hesti Utami Risky Hadi wibowo Risky Hadi Wibowo Risky Hadi Wibowo Risky Hadi Wibowo Rizal Khoirun Alfisah ROEDHY POERWANTO Sipriyadi Sipriyadi Sipriyadi Siska Tridesianti Siti Nur Jannah Sobir Sobir Sri Budiarti Poerwanto Suhartono Suhartono SUSAN SOKA SUSI RATNANINGTYAS T. Widiyanto Taruna D. Satwika TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Tri Eko WAHYONO Tri Handayani Kurniati Tri Muji Ermayanti Tri P. Priyatno Tri Panji Tri Puji Priyatno Tri Widiyanto Utut Widyastuti Wahyu Budi Kuntari Widanarni Widanarni Widiyanto, Tri Wildan Nurusallam Yadi Suryadi Yani Aryati Yohana A Dahliani Yuli Siti Fatma Yuli Siti Fatma YULIN LESTARI Yuni Puji Hastuti Yusli Wardiatno Zulfarina Zulfarina Zulfarina,