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All Journal HAYATI Journal of Biosciences MEDIA PETERNAKAN - Journal of Animal Science and Technology Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Akuakultur Indonesia Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Ilmu dan Teknologi Kelautan Tropis Jurnal Akuatika Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Medical Journal of Indonesia Jurnal AgroBiogen Current Biochemistry Journal Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) JSDH Elkawnie Journal of Tropical Biodiversity and Biotechnology JURNAL BIOLOGI INDONESIA Jurnal Biodjati Omni-Akuatika Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis ANNALES BOGORIENSES LIMNOTEK - Perairan Darat Tropis di Indonesia Biosaintifika: Journal of Biology & Biology Education Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Jurnal Riset Akuakultur Menara Perkebunan Jurnal Mangifera Edu Grouper: Jurnal Ilmiah Perikanan Makara Journal of Science Jurnal Ilmu Kehutanan Jurnal Ilmu Kehutanan
Iman Rusmana
Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Physics Veterinary

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Salmonella population in waste water treatment installation (IPAK) at Pulo Gebang DKI Jakarta Budiarti, Sri; Rusmana, Iman
Medical Journal of Indonesia Vol 7 (1998): Supplement 1
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (82.759 KB) | DOI: 10.13181/mji.v7iSupp1.1124

Abstract

[no abstract available]
Fluks Bentik dan Potensi Aktivitas Bakteri Terkait Siklus Nitrogen di Sedimen Perairan Mangrove Pulau Dua, Banten Iswantari, Aliati; Wardiatno, Yusli; Pratiwi, Niken T.M; Rusmana, Iman
JURNAL BIOLOGI INDONESIA Vol 10, No 1 (2014): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (265.728 KB) | DOI: 10.14203/jbi.v10i1.336

Abstract

Mangrove ecosystem has important role as source of nutrient particularly nitrogen in coastal area. Nitrogen (N) is alimiting factor in marine and coastal area. The aim of this research was to study benthic fluxes and potency ofbacterial activity in sediment of mangroves area, related to nitrogen cycle. This research was conducted in floodedmangroves area in Pulau Dua. The sediment and overlying water was sampled using sediment core sampler.Experimental treatment for flux analysis and sediment-slurry were conducted in three hours. Nutrient of NH3-N,NO2-N, and NO3-N and abundance of nitrifier, denitrifier, DNRA, and ammonifier were analyzed. The resultsshowed that the abundance of anaerobic bacteria was higher than aerobic bacteria. The dominance of bacterialgroups found in sediment was ammonification bacteria. The highest nutrient concentration in sediment was NH3-N. Benthic fluxes value showed higher NH3-N tends to release from the sediment to water than NO2-N and NO3-N. Generally, mangrove sediment in Pulau Dua has higher potency ofbacterial activity (Vmax and Km) in NO3reduction by anaerobic bacteria than NH3 oxidation by aerobic bacteria.Keywords: bacteria, benthic fluxes, mangrove, potency of bacterial activity, sediment
STRUKTUR KOMUNITAS BAKTERI PENGOKSIDASI AMONIA BERDASARKAN GEN amoA DI SITU SAWANGAN-BOJONGSARI, JAWA BARAT Novita, Lena; Rusmana, Iman; Widiyanto, Tri
LIMNOTEK - Perairan Darat Tropis di Indonesia Vol 21, No 1 (2014)
Publisher : Research Center for Limnology

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Salah satu permasalahan dalam pengelolaan perairan darat adalah penurunan kualitas air yang disebabkan oleh polusi senyawa nitrogen. Mekanisme transformasi senyawa nitrogen oleh bakteri indigenous menjadi langkah penting untuk mengatasi permasalahan tersebut. Aktivitas antropogenik di sekitar Situ Sawangan-Bojongsari memungkinkan terjadinya polusi senyawa nitrogen seperti amonia. Penelitian bertujuan untuk mengetahui struktur komunitas bakteri pengoksidasi amonia serta faktor fisika dan kimia yang mempengaruhinya di Situ Sawangan-Bojongsari. Kelimpahan dan keragaman bakteri pengoksidasi amonia dipengaruhi oleh faktor fisika dan kimia perairan. Kelimpahan tertinggi bakteri pengoksidasi amonia terdapat pada strata 0 cm (270 sel/mL) dan kelimpahan terendah terdapat pada strata 230 cm (73 sel/mL). Bakteri pengoksidasi amonia tidak ditemukan pada bagian sedimen. Gen amoA hanya teramplifikasi dari contoh air. Sebanyak 10 pita gen amoA dengan posisi yang berbeda dapat terdeteksi pada gel DGGE. Sebanyak enam isolat gen amoA memiliki kemiripan sekuen nukleotida dengan amo A dari uncultured bacterium (86-97%). Sekuen asam amino dari keenam isolat gen amo A menunjukkan kemiripan dengan protein ammonia monooxygenase (56-93%). Sebanyak lima isolat gen amo A teridentifikasi sebagai ammonia monooxygenase dari uncultured bacterium dan satu isolat sebagai ammonia monooxygenase dari Nitrosospira sp. III7. Berdasarkan analisis filogenetik, keenam isolat gen amoA termasuk ke dalam genus Nitrosospira.
The Effect of Biofertilizer on The Diversity of N2O Reducing Bacteria in Paddy Fields of Sukabumi, Indonesia Cahyadi, Alfan; Rusmana, Iman; Mubarik, Nisa Rachmania
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.9796

Abstract

Some of the methanotrophic bacteria and N2O reducing bacteria have been proven to be able to support the plant growth and increase the productivity of paddy. However effect of the methanotrophic and N2O reducing bacteria application as a biofertilizer to indigenous N2O reducing bacteria is still not well known yet. The aim of this study was to analyze the diversity of N2O reducing bacteria in lowland paddy soil based on a nosZ gene. Soil samples were taken from lowland paddy soils in Pelabuhan Ratu Sukabumi, West Java, Indonesia. There were two treatments for the paddy field soil, ie. biofertilizer-treated field 20% fertilizer (50 kg/ha) with the addition of biofertilizer and 100% fertilizer. PCR amplification of nosZ gene was successfully conducted using nosZF and nosZR primer pair. Denaturing Gradient Gel Electrophoresis (DGGE) process was conducted at 150 V for 5.5h. There were three differences nosZ bands were sequenced. The phylogenetic analysis showed that they were close to uncultured bacteria. Microbial diversity in the biofertilizer-treated field was higher than that of in the 100% fertilizer-treated field. The biofertilizer treatment has higher in microbial diversity than that of applied non-biofertilizer paddy fields. This research might have impact in the application of biofertilizers due to the emission of N2O as a green house gas from paddy fields farming activity. The biofertilizer has great potential application in sustainable environmental friendly agriculture systems.
Degradation of Polycyclic Aromatic Hydrocarbon Pyrene by Biosurfactant-Producing Bacteria Gordonia cholesterolivorans AMP 10 Kurniati, Tri Handayani; Rusmana, Iman; Suryani, Ani; Mubarik, Nisa Rachmania
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.6448

Abstract

Pyrene degradation and biosurfactant activity by a new strain identified as Gordonia cholesterolivorans AMP 10 were studied. The strain grew well and produced effective biosurfactants in the presence of glucose, sucrose, and crude oil. The biosurfactants production was detected by the decreased surface tension of the medium and emulsification activity. Analysis of microbial growth parameters showed that AMP10 grew best at 50gmL-1pyrene concentration, leading to 96% degradation of pyrene within 7days. The result of nested PCR analysis revealed that this isolate possessed the nahAc gene which encodes dioxygenase enzyme for initial degradation of Polycyclic Aromatic Hydrocarbon (PAH). Observation of both tensio-active and emulsifying activities indicated that biosurfactants which produced by AMP 10 when grown on glucose could lower the surface tension of medium from 71.3 mN/m to 24.7 mN/m and formed a stable emulsion in used lubricant oil with an emulsificationindex (E24) of 74%. According to the results, it is suggested that the bacterial isolates G. cholesterolivorans AMP10 are suitable candidates for bioremediation of PAH-contaminated environments.How to CiteKurniati, T. H., Rusmana, I. Suryani, A. & Mubarik, N. R. (2016). Degradation of Polycyclic Aromatic Hydrocarbon Pyrene by Biosurfactant-Producing Bacteria Gordonia cholesterolivorans AMP 10. Biosaintifika: Journal of Biology & Biology Education, 8(3), 336-343.
Identification of nifD and nifH Genes of Methanotrophic Bacteria from Rice Field Bintarti, Ari Fina; Rusmana, Iman; Wahyudi, Aris Tri
ANNALES BOGORIENSES Vol 18, No 2 (2014): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (879.425 KB) | DOI: 10.1234/97

Abstract

Metanotrophic bacteria have ability to oxidize methane and fix atmospheric nitrogen, hence the bacteria has an important role as a nitrogen source provider on wetland area like rice fields. Nitrogen fixation process is catalyzed by the nitrogenase enzyme complex, encoded by nifD and nifH genes. However, characteristic of these genes from indigenous-methanotrophic bacteria still poorly understood. Hence, nifD and nifH genes of methanotrophic bacteria isolated from rice fields in Indonesia (BGM3, BGM9, SS1, SS3, SS10, ST18, SP3 and INP4) were identified and characterized. Detection of nifH and nifD genes was conducted by polymerase chain reaction (PCR) amplification. nifH and nifD gene sequences were analyzed using BLAST-X and phylogenetic trees were constructed using Neighbour Joining method. Based on nifH sequences analysis, SS1 closely related to Beijerinckia mobilis and SS3, SS10, ST 18 closely related to Beijerinckia indica subsp. indica ATCC 9039, while, BGM3, INP4, and BGM9 related to nifH of uncultured nitrogen-fixing bacterium. In other hand, sequence analysis of nifD gene showed that SS1, SS3, SS10, ST 18 closely related to B. indica subsp. indica ATCC 9039 and BGM3, BGM9, INP4 closely related to Xanthobacter autotrophicus Py2. Identification by 16 SrRNA indicated that SS1, SS3, SS10, and ST18 had closeness to Beijerinckia sp. P310-1, while INP4 closely related to Xanthobacter sp. M5C24.
FLUKS BENTIK DAN POTENSI AKTIVITAS BAKTERI TERKAIT SIKLUS NITROGEN DI SEDIMEN PERAIRAN MANGROVE PULAU DUA, BANTEN Iswantari, Aliati; Wardiatno, Yusli; Pratiwi, Niken T.M; Rusmana, Iman
JURNAL BIOLOGI INDONESIA Vol 10, No 1 (2014): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v10i1.336

Abstract

Mangrove ecosystem has important role as source of nutrient particularly nitrogen in coastal area. Nitrogen (N) is alimiting factor in marine and coastal area. The aim of this research was to study benthic fluxes and potency ofbacterial activity in sediment of mangroves area, related to nitrogen cycle. This research was conducted in floodedmangroves area in Pulau Dua. The sediment and overlying water was sampled using sediment core sampler.Experimental treatment for flux analysis and sediment-slurry were conducted in three hours. Nutrient of NH3-N,NO2-N, and NO3-N and abundance of nitrifier, denitrifier, DNRA, and ammonifier were analyzed. The resultsshowed that the abundance of anaerobic bacteria was higher than aerobic bacteria. The dominance of bacterialgroups found in sediment was ammonification bacteria. The highest nutrient concentration in sediment was NH3-N. Benthic fluxes value showed higher NH3-N tends to release from the sediment to water than NO2-N and NO3-N. Generally, mangrove sediment in Pulau Dua has higher potency ofbacterial activity (Vmax and Km) in NO3reduction by anaerobic bacteria than NH3 oxidation by aerobic bacteria.Keywords: bacteria, benthic fluxes, mangrove, potency of bacterial activity, sediment
Candida Species Distribution of Clinical Specimens in Banda Aceh, Indonesia Suhartono, Suhartono; Mahdani, Wilda; Masthura, Aderiana; Rusmana, Iman
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 2 (2020): August 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i2.23758

Abstract

Candidiasis has become increasingly widespread in the community alongside with the developing resistance of Candida sp. to some antifungals. A prevalence study in the present research is required to surveil the distribution of Candida-related infections to administrate the appropriate antifungal treatments. The objective of this research was to determine the species distribution of candidiasis with their antifungal susceptibility isolated from clinical specimens at the Zainoel Abidin Hospital (ZAH) Banda Aceh, Indonesia. The clinical specimens were from inpatients and outpatients in the hospital during January to June 2019. The identification of Candida species and antifungal sensitivity assay were conducted by using VITEK® 2 Compact. Of a total of 68 isolates, there were six species of Candida with the highest species prevalence was Candida tropicalis (52.94%). Additionally, the highest prevalence of candidiasis came from urine specimen (54.41%) and mostly from inpatients in the internal medicine unit (54.41%). Candidiasis occurred predominantly in men (58.82%) and during adulthood (55.88%). The antibiogram of Candida sp. shows a high percentage of sensitivity towards some antifungals including fluconazole (100%), voriconazole (100%), caspofungin (100%), micafungin (100%), amphotericin B (99.5%), and flucytosine (100%). From this result, it can be concluded that candidiasis cases in the ZAH Banda Aceh, Indonesia has prevalently occurred with the few antifungal therapies for candidiasis of were still effective empirically and definitively. This is an initial study of Candida prevalence within different clinical samples in Banda Aceh and the study is expected to be a basis for prevention and control of Candida-related infections in the area.
Comparison of DNA Extraction Methods for Microbial Community Analysis in Indonesian Tempe Employing Amplified Ribosomal Intergenic Spacer Analysis CECILIA ANNA SEUMAHU; ANTONIUS SUWANTO; IMAN RUSMANA; DEDY DURYADI SOLIHIN
HAYATI Journal of Biosciences Vol. 19 No. 2 (2012): June 2012
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (136.378 KB) | DOI: 10.4308/hjb.19.2.93

Abstract

Tempe fermentation involved complex microbial communities which are only revealed partially through culture dependent methods. Culture-independent methods would be potential to unravel this complex microbial fermentation. Appropriate DNA extraction is an essential tool to obtain reliable data from culture independent method. In this study, we employed two commercial DNA extraction methods to find the best one for microbial community characterization employing amplified ribosomal intergenic spacer analysis (ARISA). Our result showed that PowerFood Microbial DNA Isolation Kit-MOBIO (PFMDIK) is an excellent method for microbial DNA extraction from tempe. It gave high quantity and quality of DNA suitable for PCR amplification of 16S-23S rRNA intergenic spacer to yield a diverse and reproducible ARISA profile.
Bacterial and Fungal Communities in Tempeh as Reveal by Amplified Ribosomal Intergenic Sequence Analysis CECILIA ANNA SEUMAHU; ANTONIUS SUWANTO; IMAN RUSMANA; DEDY DURYADI SOLIHIN
HAYATI Journal of Biosciences Vol. 20 No. 2 (2013): June 2013
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (142.073 KB) | DOI: 10.4308/hjb.20.2.65

Abstract

Tempeh is an Indonesian traditional fermented food produced using Rhizopus as a starter culture. In practice, however, the starter culture as well as fermentation processes would yield a polymicrobial fermentation, which generated a unique tempeh flavor and texture. This condition makes Indonesian tempeh as one of the most complex fermented food, while at the same time would make it difficult to scale up tempeh production with uniform quality and consistency. The aim of this study was to compare a number of tempeh microbial communities employing Amplified Ribosomal Intergenic Sequence Analysis (ARISA). Fresh tempeh samples were obtained from tempeh producers in Java and Moluccas. 16S rRNA gene libraries and DNA sequencing were employed to analyze further the nature of bacterial diversity in two selected tempeh samples. The results of our study showed that different tempeh producer possessed different Bacterial ARISA (BARISA) or fungi ARISA (FARISA) profiles.  However, BARISA profiles were found to be more discriminative than FARISA, and therefore BARISA would be more useful for tempeh genetic fingerprint or barcoding.
Co-Authors Achmad Dinoto Aliati Iswantari ALINA AKHADIYA Alina Akhdiya Alina Akhdiya ALINA AKHDIYA Amanda, Nisa Widya Andri Ferbiyanto Angela Mariana Lusiastuti Angela Mariana Lusiastuti Ani Suryani Ani Widiyati ANIK KUSMIATUN Anja Meryandini Anja Meryandini Antonius Suwanto dan Meity S. Sinaga . Budi Tjahjono Andi Khaeruni R Ari Fina Bintarti Ari Fina Bintarti, Ari Fina Aris Tri Wahyudi Baskoro S Wibowo Cahyadi Irwan Cahyadi, Alfan Cecep Kusmana CECILIA ANNA SEUMAHU Ciptadi Achmad YUSUP Daniel Djokosetiyanto Darmono Taniwiryono Desniar . Dewi Puspaningsih DIAH ISKANDRIATI Dian Hendrayanti Dinamella Wahjuningrum Dondin Sajuthi DWI ANDREAS SANTOSA Dwi Andreas Santosa Dwi Ningsih Susilowati Eddy Supriyono Emei Widiyastuti Fachriyan Hasmi Pasaribu Fachriyan Hasmi Pasaribu Faturrahman Faturrahman Fredinan Yulianda Fredinan Yulianda Frijona Fabiola Lokollo GALUH WENING PERMATASARI Gunawan Djajakirana Hamim Hamim Happy Widiastuti Hefni Effendi Hessy Novita Hessy Novita I Komang Gede Wiryawan I MADE ARTIKA I Made Samudra Ifa Manzila It Jamilah KATHARINA JESSICA Khairul Syahputra Kukuh Nirmala Lena Novita Lizawati Lizawati Maggy Thenawidjaya Maggy Thenawidjaya Suhartono Maggy Thenawidjaya Suhartono Mahdani, Wilda Mahyarudin, Mahyarudin Masrukhin Masrukhin Masthura, Aderiana Miftahudin . Mukti Ali Munti Yuhana MUTTAQIN, MAFRIKHUL Niken T.M Pratiwi, Niken T.M NISA RACHMANIA MUBARIK Nisa Widya Amanda Novita, Lena PRIHASTO SETYANTO Prihasto Setyanto Priyo Handoyo Wicaksono Rahman Rahman Rahman Rahman Rahminovita Herlis RATNA SETYANINGSIH RATNA SETYANINGSIH RIDWAN AFFANDI RIKA RAFFIUDIN Rina Hesti Utami Risky Hadi wibowo Risky Hadi Wibowo Risky Hadi Wibowo Rizal Khoirun Alfisah ROEDHY POERWANTO Sipriyadi Sipriyadi Sipriyadi Siska Tridesianti Siti Nur Jannah Sobir Sobir Solly Aryza Sri Budiarti Poerwanto SUSAN SOKA SUSI RATNANINGTYAS Syaiful Anwar T. Widiyanto Taruna D. Satwika TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Tri Eko WAHYONO Tri Handayani Kurniati Tri Muji Ermayanti Tri P. Priyatno Tri Panji Tri Puji Priyatno Tri Widiyanto Utut Widyastuti Wahyu Budi Kuntari Widanarni Widanarni Widiyanto, Tri Wildan Nurusallam Yadi Suryadi Yani Aryati Yohana A Dahliani Yohanna Anisa Indriyani Yuli Siti Fatma Yuli Siti Fatma YULIN LESTARI Yuni Puji Hastuti Yusli Wardiatno Zulfarina Zulfarina Zulfarina,