Indratin Indratin
Peneliti Badan Penelitian dan Pengembangan Pertanian, Balai Penelitian Lingkungan Pertanian Jl. Raya Jakenan-Jaken KM 05 Jakenan Pati

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Bioremediasi untuk Menurunkan Kadar Insektisida Klordan di Lahan Sawah Indratin, Indratin; Wahyuni, Sri; Setyanto, Prihasto
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 13, No 1 (2016): Prosiding Seminar Nasional XII Biologi
Publisher : Universitas Sebelas Maret

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Bioremediation   is   one   of the effective   ways   to   remediate   pesticide-contaminated   land,   in order to   reduce environmental pollution problems. Bacillus substillis, Heliothrix oregonensis, Catenococcus thiocycli,and Achoromobacter sp are soil microbes which are capable of lowering the levels of chlordanein the fields. These microbes derived from preliminary research results indigenous soil insulation in the Laboratory of Microbiology LIPI Cibinong. This research is aimed to determine the decrease levels of chlordane in paddy fields cultivated with rice Ciherang variety using bioremediation treatment. The soil used for the planting medium is taken from Cilamaya village, District CilamayaWetan,   Karawang.  This research   is conducted   at   Jakenan Research Station from January to December 2013. This research uses  activated carbon coated urea and biochar enriched by   indigenous  microbes with  a dose 250 kg/ha. This research is designed by using  complete   randomized   block  design  which   is   repeated   three times with for 40 ml microbial concorcia populations 109 in 1 kg of urea coated by biochar or activated carbon. The microplot size 1 x 1 m with spacing of 20 x 20 cm. Insecticide residue analyzes carried out in Balingtan Laboratory in Bogor by gas chromatography, using the SNI 06-6991.1-2004method. The result shows  biochar coconutshell-coated  urea   enriched   with  microbes   in  rice   farming   can  reduce   pesticide  residues   chlordane amountedto 27.10%
Urea Coating with Activated Carbon Enriched by Microbial Indigenous Can Reduce Endrin Concentration Wahyuni, Sri; Indratin, Indratin; Dewi, Widyatmani Sih; Atmanto, Heru
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 12, No 1 (2015): Prosiding Seminar Nasional XII Biologi
Publisher : Universitas Sebelas Maret

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Endrin residues are still remain in the  land field these compounds are no longer used by farmers and have been banned by the government. This residue can stay in the soil longer and persistant. Microbial enrichment is expected to accelerate the degradation of pesticide residues. Microbes stretcher are Bacillus substillis, Heliothrix oregonensis, Catenococcus thiocycli, and Achoromobacter sp obtained from the preliminary research results  from soil isolation of idegenus in LIPI Cibinong Microbiology Laboratory.  Soil for the planting medium obtained from the village of Karawang, Regency Cilamaya Wetan, Cilamaya District. The experiment was conducted in the field by using lysimeter at the Experiment Jakenan station from July 2013 to December 2013. The objective of the research is to obtain technology of activated carbon-coated urea and biochar which  enriched microbial indegenus. The experiment was used randomized block design (RAK) with 3 replications.  Plant used are rice. Insecticide residue analysis was carried out in the laboratory in Bogor Balingtan using gas chromatography (GC), with the SNI method 06-6991.1-2004. The purpose of this study knowing the capabilities of urea coating with activated carbon enrichment microbia in reducing the concentration of residues endrin. Results of research urea coated activated carbon from coconut shell are enriched with microbes on paddy field can lower pesticide residues of endrin respectively to 33.6%.  This is presumably due to the role of microbes degrading effective as activated carbon as the preferred home. Enrichmentwith microbial indegenuscan improve theeffectivenessof ureacoating biochar andureacoatingactivated carbon coconut shell todecrease concentration endrin.
Degradation of Cypermethrin by Indigenous Bacteria from Contaminated Soil Indratin, Indratin; Kurnia, Asep; Wahyuni, Sri
Makara Journal of Science
Publisher : UI Scholars Hub

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Soil contamination by pesticides should be controlled by using soil microbes with the ability to degrade pesticide resi-dues. Microorganisms that have adapted to a particular pesticide could accelerate the degradation process. The present study aimed to select bacteria in soil that could potentially degrade cypermethrin residues. Experiments were carried out in a laboratory and employed soil samples collected from the districts of Demak, Magelang, and Brebes. Each soil sample was initially tested for the presence of bacteria and pesticide residues. Indigenous microbes capable of living in contaminated environments were adapted to cypermethryn and cultured in the laboratory. Experiment was carried out in several stages, namely, (1) isolation and identification of microbes that could degrade cypermethrin, (2) testing of the growth characteristics of isolates in cypermethrin, and (3) determination of cypermethrin residues in cultures. Three isolates, namely Pseudomonas alcaligenes, Bacillus amyloliquenfaciens, and Pseudomonas aeruginosa, were found to decrease cypermethrin residues by up to 95% with fast half-lives and good growth capability. The results demonstrate the potential applications of the isolates in biodegradation processes and remediation of cultivation fields.