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R. Indriani
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Biochemistry, Genetics & Molecular Biology Veterinary

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Pemetaan Genetik Virus Avian Influenza di Indonesia 2007 Dharmayanti, NLP Indi; Indriani, R.; Hartawan, R.; Hewajuli, D.A.; Ratnawati, A.; Darminto, Darminto
JURNAL BIOLOGI INDONESIA Vol 5, No 2 (2008): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (667.831 KB) | DOI: 10.14203/jbi.v5i2.3195

Abstract

ABSTRACTGenetic Mapping of Indonesian Avian Influenza Viruses 2007.Department of agricultureimplements vaccination as one a tool to control of avian influenza disease. The vaccinationprogram use virus seed such as H5N1, H5N2 and H5N9. Such as vaccination program for otherdiseases, avian influenza vaccine program have lack implementation in the field. In 2007,department of agriculture evaluated the AI vaccination program such as the master seedvaccine that can be used. Result of the evaluation showed that 11 of AI vaccines that weredistributed in Indonesia did not give protection more than 60% to Indonesian isolates in 2006(A/Ck/Pwt-Wij/06). From this point and many AI cases in the field in 2007, the aim of thisstudy was to conduct genetic diversity of avian influenza viruses which have circulated inIndonesia region. We used virus isolation for propagate the viruses, RT-PCR for identificationand DNA sequencing on HA1 region to analysis genetic diversity for genetic mapping anduseful for master seed candidate. The result of the study showed that there were 6 group ofgenetic diversity in 2007; Isolates from group 1, 5 and 6 can be used for AI vaccine candidate.Key words: genetic mapping, genetic diversity and avian influenza viruses
Kemampuan Primer General CK4/CK2 Virus Infectious Bronchitis (IBV) untuk Mengaplifikasi Genom IBV Isolat Lapang Indonesia Dharmayanti, N.L P.I.; Asmara, W.; Artama, W. T.; Indriani, R.; Darminto, Darminto
JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v3i3.3473

Abstract

ABSTRACTCapability of general primer CK4/CK2 infectious bronchitis virus (IBV) to amplify of genomic IBV field isolates in Indonesia. Avian infectious bronchitis virus (IBV), the prototype of coronaviridae, is highly contangius, economically important pathogen of the chickens. Comparative sequence analysis of IBV S1 gene revealed regions that were conserved among serotypes. Two of conservesd region were used to develop degenarate general primer CK4/CK2 for amplifying IBV genomic RNA by RT-PCR. The aims of this study to demonstrated ability of general primer CK4/CK2 for amplified S1 gene IBV field isolates in Indonesia. The result of this is study suggest that only three IBV field isolates that can be amplified by primer CK4/CK2 that are I-14, I-37 and I-269.Key words: Capability, general primer CK4/CK2, infectious bronchitis virus, field isolates
Potensi Virus A/Ck/West Java/PWT-Wij/2006 Sebagai Vaksin Indriani, R.; Dharmayanti, NLP; Adjid, R.M.A.; Darminto, Darminto
JURNAL BIOLOGI INDONESIA Vol 7, No 2 (2011): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (158.541 KB) | DOI: 10.14203/jbi.v7i2.3117

Abstract

ABSTRACTPotential virus A/Ck/West Java/PWT-Wij/06 for Vaccin. Vaccination program for controllingavian influenza (AI) virus infection in poultry have been emerged during the past 5 years inIndonesia. However, due to the mutation character of this virus the available vaccines were nolonger effective. Therefore a new local isolate of avian influenza virus A/Ck/West Java/Pwt-Wij/2006 was studied. The virus, formulated as inactive vaccine, was injected in to 3 weeks oldof layer chickens intramuscularly. At 3 weeks after vaccination, vaccinated chickens werechallenged against seven local isolates of HPAI H5N1 intranasaly. Unvaccinated chickenswere included in the challenge test as control. Results showed that the vaccine produced100% protection against A/Ck/West Java/Pwt-Wij/2006 (homologous), A/Ck/West Java/1067/2003, A/Ck/West Java/Hj-18/2007, and A/Ck/Payakumbuh/BPPVRII/2007; produced 90%protection against A/Ck/BB149/5/2007, and 80% protection against A/Ck/West Java/Hamd/2006 isolates. The vaccine also stoppped viral shedding by day 5 to 7 after challange. Thisstudy indicate that the new local isolate of avian influenza A/Ck/West Java/Pwt-Wij/2006 hasgood potency as vaccine with broad spectrum against a range of AI viruses available inIndonesia.Key words: avian influenza, HPAI, H5N1,vaccine, poultry.
Isolasi dan Identifikasi Wabah Avian Influenza pada Bulan Oktober 2004-Maret 2005 di Indonesia Dharmayanti, NLP. I.; Indriani, R.; Damayanti, R.; Wiyono, A.
JURNAL BIOLOGI INDONESIA Vol 3, No 9 (2005): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1043.223 KB) | DOI: 10.14203/jbi.v3i9.3277

Abstract

ABSTRACTIsolation and Identification of Avian Influenza Outbreak in October 2004 - March 2005in Indonesia. Recent outbreak of avian influenza A (HSNI) in poultry throughout Asiaincluding Indonesia caused major economic problems. Birds infection with this virus wasidentified in Indonesia in January 2004. Since March 2004, the avian influenza cases hasdecreased, but then in October 2004 was reported that outbreak has occurred in some districtin Indonesia. This study is the first to isolation and identification the avian influenza outbreakin Indonesia in October 2004 until March 2005. Our finding revealed that outbreak of poultrydisease in this period was caused by an avian influenza H5 subtype.Keywords: Identification, virus avian influenza subtype H5
ISOLASI DAN IDENTIFIKASI WABAH AVIAN INFLUENZA PADA BULAN OKTOBER 2004-MARET 2005 DI INDONESIA Dharmayanti, NLP. I.; Indriani, R.; Damayanti, R.; Wiyono, A.
JURNAL BIOLOGI INDONESIA Vol 3, No 9 (2005): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v3i9.3277

Abstract

ABSTRACTIsolation and Identification of Avian Influenza Outbreak in October 2004 - March 2005in Indonesia. Recent outbreak of avian influenza A (HSNI) in poultry throughout Asiaincluding Indonesia caused major economic problems. Bird's infection with this virus wasidentified in Indonesia in January 2004. Since March 2004, the avian influenza cases hasdecreased, but then in October 2004 was reported that outbreak has occurred in some districtin Indonesia. This study is the first to isolation and identification the avian influenza outbreakin Indonesia in October 2004 until March 2005. Our finding revealed that outbreak of poultrydisease in this period was caused by an avian influenza H5 subtype.Keywords: Identification, virus avian influenza subtype H5
PEMETAAN GENETIK VIRUS AVIAN INFLUENZA DI INDONESIA 2007 Dharmayanti, NLP Indi; Indriani, R.; Hartawan, R.; Hewajuli, D.A.; Ratnawati, A.; Darminto, Darminto
JURNAL BIOLOGI INDONESIA Vol 5, No 2 (2008): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v5i2.3195

Abstract

ABSTRACTGenetic Mapping of Indonesian Avian Influenza Viruses 2007.Department of agricultureimplements vaccination as one a tool to control of avian influenza disease. The vaccinationprogram use virus seed such as H5N1, H5N2 and H5N9. Such as vaccination program for otherdiseases, avian influenza vaccine program have lack implementation in the field. In 2007,department of agriculture evaluated the AI vaccination program such as the master seedvaccine that can be used. Result of the evaluation showed that 11 of AI vaccines that weredistributed in Indonesia did not give protection more than 60% to Indonesian isolates in 2006(A/Ck/Pwt-Wij/06). From this point and many AI cases in the field in 2007, the aim of thisstudy was to conduct genetic diversity of avian influenza viruses which have circulated inIndonesia region. We used virus isolation for propagate the viruses, RT-PCR for identificationand DNA sequencing on HA1 region to analysis genetic diversity for genetic mapping anduseful for master seed candidate. The result of the study showed that there were 6 group ofgenetic diversity in 2007; Isolates from group 1, 5 and 6 can be used for AI vaccine candidate.Key words: genetic mapping, genetic diversity and avian influenza viruses
KEMAMPUAN PRIMER GENERAL CK4/CK2 VIRUS INFECTIOUS BRONCHITIS (IBV) UNTUK MENGAPLIFIKASI GENOM IBV ISOLAT LAPANG INDONESIA Dharmayanti, N.L P.I.; Asmara, W.; Artama, W. T.; Indriani, R.; Darminto, Darminto
JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v3i3.3473

Abstract

ABSTRACTCapability of general primer CK4/CK2 infectious bronchitis virus (IBV) to amplify of genomic IBV field isolates in Indonesia. Avian infectious bronchitis virus (IBV), the prototype of coronaviridae, is highly contangius, economically important pathogen of the chickens. Comparative sequence analysis of IBV S1 gene revealed regions that were conserved among serotypes. Two of conservesd region were used to develop degenarate general primer CK4/CK2 for amplifying IBV genomic RNA by RT-PCR. The aims of this study to demonstrated ability of general primer CK4/CK2 for amplified S1 gene IBV field isolates in Indonesia. The result of this is study suggest that only three IBV field isolates that can be amplified by primer CK4/CK2 that are I-14, I-37 and I-269.Key words: Capability, general primer CK4/CK2, infectious bronchitis virus, field isolates
POTENSI VIRUS A/CK/WEST JAVA/PWT-WIJ/2006 SEBAGAI VAKSIN Indriani, R.; Dharmayanti, NLP; Adjid, R.M.A.; Darminto, Darminto
JURNAL BIOLOGI INDONESIA Vol 7, No 2 (2011): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v7i2.3117

Abstract

ABSTRACTPotential virus A/Ck/West Java/PWT-Wij/06 for Vaccin. Vaccination program for controllingavian influenza (AI) virus infection in poultry have been emerged during the past 5 years inIndonesia. However, due to the mutation character of this virus the available vaccines were nolonger effective. Therefore a new local isolate of avian influenza virus A/Ck/West Java/Pwt-Wij/2006 was studied. The virus, formulated as inactive vaccine, was injected in to 3 weeks oldof layer chickens intramuscularly. At 3 weeks after vaccination, vaccinated chickens werechallenged against seven local isolates of HPAI H5N1 intranasaly. Unvaccinated chickenswere included in the challenge test as control. Results showed that the vaccine produced100% protection against A/Ck/West Java/Pwt-Wij/2006 (homologous), A/Ck/West Java/1067/2003, A/Ck/West Java/Hj-18/2007, and A/Ck/Payakumbuh/BPPVRII/2007; produced 90%protection against A/Ck/BB149/5/2007, and 80% protection against A/Ck/West Java/Hamd/2006 isolates. The vaccine also stoppped viral shedding by day 5 to 7 after challange. Thisstudy indicate that the new local isolate of avian influenza A/Ck/West Java/Pwt-Wij/2006 hasgood potency as vaccine with broad spectrum against a range of AI viruses available inIndonesia.Key words: avian influenza, HPAI, H5N1,vaccine, poultry.
Co-Authors A. Wiyono Darminto . Dharmayanti, N.L P.I. Dharmayanti, N.L P.I. Dharmayanti, NLP Dharmayanti, NLP Dharmayanti, NLP. I. Dharmayanti, NLP. I. Hartawan, R. Hartawan, R. Hewajuli, D.A. Hewajuli, D.A. N.L.P.I Dharmayanti NLP Indi Dharmayanti R. Damayanti, R. R.M.A Adjid R.M.A. Adjid Ratnawati, A. Ratnawati, A. Rini Damayanti W. Asmara W. T. Artama, W. T.