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13. The Amount of Melanomacrophage centres (MMC) in Liver and Kidneys of Tilapia (Oreochromis niloticus) Maintained in Various Population Density Nurul Hadi; Dwina Aliza; Razali Daud
Jurnal Medika Veterinaria Vol 11, No 2 (2017): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (275.883 KB) | DOI: 10.21157/j.med.vet..v11i2.4379

Abstract

The aim of this research was to find out the amount of melanomacrophage centres (MMC) in liver and kidney of nile tilapia (Oreochromis niloticus) fish reared in high dense population. Fifty nile tilapia were grouped in four treatment groups, group I contain 5 fish, group II contain 10 fish, group III contain 15 fish and group IV contain 20 fish. Treatment was carried out for 24 hours. Fish was necropsied to collect the liver and kidney, fixed in NBF 10% then proceed to histopathological method using hematoxilin and eosin for staining. Histopathological observation was done microscopically, and MMC was captured by photomicrograph. The data were analyzed descriptively. The result showed that the average of MMC number in fish liver in group K1, K2, K3, and K4 were 9.6, 11.3, 12.6, and 13.2. While the average of MMC number in fish kidney were 5.5, 7.7, 8.5, and 9.2, respectively. The higher the dense of population the higher the amount of MMC found in liver and kidney of nile tilapia.
KORELASI ANTARA DEHIDRASI DENGAN TOTAL PROTEIN PLASMA, HEMOGLOBIN, DAN PACKED CELL VOLUME PADA KAMBING KACANG UMUR 10-14 HARI Roslizawaty -; Sugito -; Syinta Ramadhani; Muhammad Hasan; Razali Daud; Nuzul Asmilia
Jurnal Medika Veterinaria Vol 9, No 1 (2015): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (248.31 KB) | DOI: 10.21157/j.med.vet..v9i1.2982

Abstract

Penelitian ini bertujuan mengetahui korelasi antara kadar packed cell volume, total protein plasma, dan hemoglobin dengan tingkat dehidrasi  pada anak kambing kacang. Sampel penelitian ini adalah 10 ekor anak kambing kacang berumur 10-14 hari, berjenis kelamin jantan, dan tidak cacat fisik. Dehidrasi diinduksi dengan pemberian diuretik yaitu furosemide 2 mg/kg bobot badan secara intramuskular setiap empat jam, spironolactone 50 mg dan hydroclorothiazide 50 mg masing-masing diberikan per oral setiap delapan jam, dan larutan katartika (sukrosa 2 gr/kgbobot badan) di dalam larutan sukrosa 20% diberikan per oral setiap delapan jam selama 12 jam. Analisis yang digunakan adalah analisis regresi linear sederhana. Derajat dehidrasi ditentukan berdasarkan persentase penurunan berat badan. Hasil penelitian menunjukkan bahwa total protein plasma (R2= 0,929) mempunyai hubungan yang erat dengan derajat dehidrasi, hemoglobin (R2 =0,236), dan packed cell volume (R2 = 0,203) mempunyai hubungan yang rendah dengan derajat dehidrasi.
GAMBARAN HISTOPATOLOGIS LIMPA TIKUS PUTIH (Rattus novergicus) JANTAN DIINFEKSI Trypanosoma evansi DAN DIBERI EKSTRAK KULIT BATANG JALOH (Salix tetrasperma Robx) (Histopathological finding of Rat (Rattus novergicus) Spleen Infected with Trypanosoma evansi and Treated with Willow Bark (Salix tetrasperma Roxb) Extract) Elsa Mariane Ramadani; Hamdani Budiman; Sugito Sugito; Ummu Balqis; Muhammad Hambal; Razali Daud
Jurnal Medika Veterinaria Vol 9, No 2 (2015): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v9i2.3811

Abstract

The aim of this research was to determine the histopathological finding of rat (Rattus novergicus) spleen infected with Trypanosoma evansi and treated with willow bark (Salix tetrasperma Roxb) extract.This research used 25 male rats divided into four treatment groups and fed ad libitum. Group 0 (K0) was control group, group I (K1) was infected with 103/0.3 ml. T. evansi, group II (K2), III (K3), and IV (K4) were infected with103/0.3 ml T. evansi and treated with willow bark extract 30, 45, and 60 mg/kg bw, respectively. All groups were treated for 3 consecutive days orally using a stomach tube. On day four, rats were euthanized using chloroform and necropsied, then spleen were collected for histophatological examination. The results showed that all spleen on group K1, K2, K3, and K4 showed hyperplasia of white pulp, hemosiderin, and necrosis with different severity levels compared to normal spleen structure. In conclusion, the administration of willow bark extract at the dose of 30 mg/kg bw has a potency to prevent spleen from T. evansi infection. However, the higher the dose of willow bark extract the higher the level of spleen damage.Key words: spleen, T. evansi, willow bark extract
10. Isolation and identification of Aeromonas sp in goldfish (Carrasius auratus) from several pet shops in Banda Aceh Hamny Hamny; Muhammad Zulfan; Mahdi Abrar; Maryulia Dewi; Darmawi Darmawi; Razali Daud; Budianto Panjaitan
Jurnal Medika Veterinaria Vol 13, No 1 (2019): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (635.519 KB) | DOI: 10.21157/j.med.vet..v13i1.4225

Abstract

The aim of this study was to isolate and identify Aeromonas sp from pet shops in Banda Aceh. A total of 15 goldfish were obtained from 3 pet shop in Banda Aceh and examined at the Laboratory of Microbiology, Faculty of Veterinary Medicine, University of Syiah Kuala in Banda Aceh. Isolation of bacteria obtained from liver, kidneys and spleen were cultured in the media Rimler-Shotts (RS) Medium Base. The bacteria were identified based on morphology of the growing bacteria, Gram stain, IMViC test, and biochemical tests. The results showed that there were no Aeromonas sp in goldfish (Carrasius auratus) from three pet shops in Banda Aceh.
19. Identification of Ectoparasites in the Mud Crab (Scylla serrata) from Alue Naga Coastal Waters Banda Aceh Audi Maldini; Winaruddin Winaruddin; Muttaqien Bakri; Muhammad Hambal; Farida Farida; Razali Daud
Jurnal Medika Veterinaria Vol 11, No 2 (2017): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (241.625 KB) | DOI: 10.21157/j.med.vet..v11i2.5156

Abstract

This study aimsed  to determine the type of ectoparasites on crab (Scylla serrata) in the water of Alue Naga Banda Aceh.  The samples of this study were 10 heads of mud crabs were taken from the Alue Naga waters Banda Aceh and taken to the Laboratory of Parasitology. Veterinary Medicine Faculty of Syiah Kuala University Banda Aceh to identify the take ectoparasites on mud crab (Scylla serrata) by observing the outer body of the organism, then noted bleeding, wounds or swelling and pay attention to the type of organism that is attached to the body of mud crab, then dredge the outer parts of the body like the carapace, foot path, foot pool and gills were take it and then tweezers and then putting the object glass that has been provided and observed with a microscope with 100 times magnification and see what kind of ectoparasites that have been found and then compare with the identification key. From the results of this study concluded that ectoparasites which infects mud crab (Scylla serrata) in the waters of Alue Naga Banda Aceh come  from genus Chilodonella sp. Whichare predilections in the gills with a prevalence rate of 10%.
IDENTIFIKASI PARASIT PADA IKAN NILA (Oreochromis niloticus) DI IRIGASI BARABUNG KECAMATAN DARUSSALAM ACEH BESAR (Identification of Parasites on Nile Tilapia (Oreochromis niloticus) Fish Collected from Barabung Irigation Darussalam Aceh Besar) Nova Lianda; Yudha Fahrimal; Razali Daud; Rusli Rusli; Dwinna Aliza; Mulyadi Adam
Jurnal Medika Veterinaria Vol 9, No 2 (2015): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (42.194 KB) | DOI: 10.21157/j.med.vet..v9i2.3807

Abstract

This study was aimed to identify parasite types that infected Nile tilapia (Oreochromis niloticus) fish collected from Barabung irigation Darussalam, Aceh Besar. A total of 26 Nile tilapia fish were collected from Barabung irigation then examined for the presence of ectoparasite and endoparasite at Parasitology Laboratory, Veterinary Medicine Faculty, Syiah Kuala University Banda Aceh. The ectoparasite examination was conducted by observed the external body part of fish macroscopically and microscopically. While endoparasite examination was conducted by observed the inner surface of digestive track. The results showed that four types of parasites were found namely Dactylogyrus sp., Gyrodactylus sp., Camallanus sp., and P. nagpurensis. Overall,73.1% Nile tilapia fish was infested by Dactylogyrus sp., 11.3% was infested by Gyrodactylus , 10 % was infested by Camallanus, and 5% was infested with P. nagpurensis.Key words: endoparasite, ectoparasite, tilapia fish
16. Healing Process Of Burns (Vulnus combustion) Degrees IIB Using Mixed Leaf (Spondias dulcis F.) Fresh And Dry With Vaselin In Rats (Rattus Norvegicus) Ummu Balqis; Mirna Safrani Fauzi; Zuhrawati NA; Nazaruddin Nazaruddin; Razali Daud; Abdullah Hamzah; Darniati Darniati
Jurnal Medika Veterinaria Vol 13, No 1 (2019): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (754.012 KB) | DOI: 10.21157/j.med.vet..v13i1.4310

Abstract

           The aims of this research was looked to determine the healing process of burns  Grade II B using a mixture of fresh and dried leaf kedondong with vaseline white rats (Rattus norvegicus). Animals used were 18 rats. This study was designed using three treatment groups namely (KI) vaseline, (KII) mixture of crushed leaves and fresh kedondong (KIII) mixture of crushed dried leaves kedondong and each two replications. Burns made on the backs of mice and the treatments are done twice a day for 21 days.Observation research parameter is the description of macroscopic and microscopic observed at day 7, 14, and 21 in the skin tissue with hematoxylin and eosin staining. The observation of macroscopic studies showed that the formation of a reddish color, presence of edema, and loss of the fastest consecutive scab is KI, KIII, and KII, while the formation of a scab fastest respectively KIII, KI and KII. The observation of histopathology showed on day 7, KI, KII and KIII found inflammatory cell infiltration, hemorrhage and edema with the spread of many.on the 14th day of KI and KII infiltration of inflammatory cells and their udema much, whereas KIII inflammatory cell infiltration decreased, and hyperemia increase, on the 21st day KI, KII and KII inflammatory cell infiltration, hyperemia and hemorrhage spread with a little, but KI and KII oedemanya still being spread. Based on the results of this study concluded that KIII accelerate the healing process of burns on rats
PENGARUH PENINGKATAN SUHU TERHADAP JUMLAH ERITROSIT IKAN NILA (Oreochromis niloticus) Puja Cikal Bangsa; Sugito -; Zuhrawati -; Razali Daud; Nuzul Asmilia; Azhar -
Jurnal Medika Veterinaria Vol 9, No 1 (2015): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (144.789 KB) | DOI: 10.21157/j.med.vet..v9i1.2985

Abstract

Penelitian ini bertujuan mengetahui pengaruh peningkatan suhu terhadap jumlah eritrosit ikan nila. Sampel yang digunakan adalah darah ikan nila yang berasal dari 18 ekor ikan nila dengan bobot badan berkisar 40-50 g. Rancangan penelitian yang digunakan adalah rancangan acaklengkap (RAL) pola searah dengan tiga perlakuan dan enam kali ulangan. Perlakuan P1 sebagai kontrol merupakan perlakuan dengan suhu akuarium 29±1°C, P2 merupakan perlakuan dengan suhu akuarium 32±1°C, dan P3 merupakan perlakuan dengan suhu akuarium 35±1°C. Ukuran akuarium pada masing-masing kelompok perlakuan adalah 80 x 60 x 40 cm dengan ketinggian air 30 cm. Perlakuan dilakukan selama 15hari dan pada hari ke-16 dilakukan pengambilan sampel darah. Darah diambil melalui vena caudalis. Data dianalisis dengan analisis varian(ANAVA) dan dilanjutkan dengan uji Duncan. Rata-rata (±SD) jumlah eritrosit ikan nila (106/mm3) pada P1, P2, dan P3 masing-masing adalah 3,01x106/mm3; 2,63x106/mm3; dan 2,08x106/mm3. Hasil penelitian menunjukkan bahwa peningkatan suhu berpengaruh (P0,05) terhadap jumlaheritrosit ikan nila. Dapat disimpulkan bahwa peningkatan suhu air 32±1°C dan 35±1°C dalam akuarium dapat menurunkan jumlah eritrosit ikan nila.
PENGARUH PENGGUNAAN GETAH BATANG PISANG KEPOK (Musaparadisiaca forma typica) TERHADAP PENYEMBUHAN LUKA OPERASI PADA ANJING LOKAL (Canis familiaris) Razali Daud
Jurnal Medika Veterinaria Vol 9, No 1 (2015): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (123.709 KB) | DOI: 10.21157/j.med.vet..v9i1.2996

Abstract

Penelitian ini bertujuan mengetahui potensi getah batang pisang kepok dalam proses penyembuhan luka operasi. Penelitian ini menggunakan 10 ekor anjing lokal (Canis familiaris) sehat yang berumur rata-rata 6 bulan dengan bobot badan 6-7 kg. Sebelum perlakuan dilaksanakan, semua anjing diadaptasikan selama tujuh hari di dalam kandang individu, setiap anjing dilakukan operasi laparotomi. Anjing dibagi dalam dua kelompok secara acak. Kelompok I diinjeksi penisilin-G dan dioleskan salep oksitetrasiklin pagi dan sore sebagai kontrol, kelompok II dioleskan getah batang pisang kepok pagi dan sore hari sebagai perlakuan. Pengamatan dilakukan setiap hari pada pagi hari sampai luka operasi sembuh. Data yang diperoleh dianalisis secara deskriptif berdasarkan pengamatan makroskopis terhadap luka kelompok I dan dibandingkan dengan kelompok II berdasarkan adanya kemerahan, kebengkakan, dan pertautan tepi luka. Pada kelompok II terjadi penyembuhan luka operasi yang sama dengan kelompok I. Dapat disimpulkan bahwa, penggunaan getah batang pisang kepok dapat menyembuhkan luka, sama halnya dengan proses kesembuhan luka yang menggunakan injeksi penisilin-G dan dioleskan salep oksitetrasiklin.
PENGARUH PEMBERIAN KACANG PANJANG (Vigna unguiculata) TERHADAP KADAR GLUKOSA DARAH MENCIT (Mus musculus) YANG DIINDUKSI ALOKSAN (Effect of Long Bean (Vigna unguiculata) on Blood Glucose Level of Mice (Mus musculus) Induced by Alloxan) Intan Fitri Aprila; M. Nur Salim; Razali Daud; T. Armansyah; Nuzul Asmilia; Faisal Jamin
Jurnal Medika Veterinaria Vol 9, No 2 (2015): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (160.992 KB) | DOI: 10.21157/j.med.vet..v9i2.3983

Abstract

The aim of this research was to find out the effect of long bean (Vigna unguiculata) on level of blood glucose on mice (Mus musculus) induced by alloxan. Twelve mice with the weigh of 25-40 grams and clinically healthy were used in this research. All mice were randomly divided into 4 treatment groups, 3 mice each. K0 was negative control group, only given aquadest. K1, K2, and K3 groups were induced by alloxan 0.5 ml. K1 group was positive control. K2 group was fed with 100 grams long bean mixed with 50 ml distilled water. K3 group was fed with 100 grams long bean mixed with 100 ml distilled water. Long bean was fed orally 0.5 ml every morning and evening for 7 consecutive days. On day eight the examination of blood glucose level was performed. The average level of blood glucose were K0 (142.00±23.39), K1 (167.00±10.54), K2 (122.67±12.50), dan K3 (154.67±16.26) mg/dL. In conclusion, the administration of long bean for seven consecutive days does not decrease blood glucose level on mice induced by alloxan.Key words: blood glucose, alloxan, long bean