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All Journal Jurnal Medika Veterinaria
Darmawi Darmawi
Laboratorium Mikrobiologi Fakultas Kedokteran Hewan Universitas Syiah Kuala
Health Professions

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12. Antibacterial Activity of Ethanol Extract of Siamih Leaf (Ageratum conyzoides) on Staphylococcus aureus bacteria divina dinda hayati; Herrialfian Herrialfian; M. Isa; Darmawi Darmawi; Fakhrurrazi Fakhrurrazi; Abdul Harris
Jurnal Medika Veterinaria Vol 14, No 1 (2020): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v14i1.10333

Abstract

The aim of this research is to know the effect of siamih leaf ethanol extract (Ageratum conyzoides) on the growth of Staphylococcus aureus bacteria. The bacteria which is  used are obtained from the Microbiology Laboratory of the Faculty of Veterinary Medicine of Syiah Kuala University on isolate bacteria of the honey bear wound (Helarctos malayanus). Bacterial identification is done by culturing the isolate on lateral NA media then it is planted on MSA media and  staining Gram, catalase test and hemolysis test. The extraction is done by maceration with 96% ethanol solvent. Extract of Siamih leaf  is done by phytochemical test. The results of pythritic test of Siamih leaf show that Siamih leaf contains alkaloid compounds, steroids, terpenoids, flavonoids and tannins. The extract concentration which is used is 20%, 40%, 60% and 80%. Nergative control by using aquades and positive controls using Amoxicillin with three repetitions. The method used is disc diffusion or Kirby-Bauer method. The results show that Siamih leaf ethanol extract inhibit the growth of Staphylococcus aureus bacteria at concentrations of 20% (0,7 mm), 40% (1,4 mm), 60% (2,2 mm), and 80 % (3,0 mm). Thus it can be concluded that the extract of siamih leaf ethanol has inhibitory power to Staphylococcus aureus bacteria with low category.
7. Inhibition Activity of Ethanolic Extract of Binahong Leaf (Anredera cordifolia (Ten.) Steenis) on Staphylococcus aureus Bacteria Herrialfian Herrialfian; Mizla Maulia Nisya Lubis; Darmawi Darmawi; Maryulia Dewi; Erina Erina; Hennivanda Hennivanda; Abdul Harris
Jurnal Medika Veterinaria Vol 15, No 1 (2021): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v15i1.9988

Abstract

This research aimed to investigate the inhibition activity of ethanolic extract of binahong leaf (Anredera cordifolia (Ten.) Steenis) on Staphylococcus aureus. Bacteria were obtained from the Microbiology Laboratory of Faculty of Veterinary Medicine Unsyiah, isolated from the honey bear (Helarctos malayanus) wound area. This experiment was conducted with re-identification of the bacteria before treatment. Afterward, the bacteria were treated with ethanolic extract of binahong leaf with a concentration of 20%, 40%, 60%, and 80% by using the disc diffusion (Kirby-Bauer) method. The data were analyzed descriptively by measuring and comparing the inhibition zone among treatments. The results showed that the treatment could inhibit the growth of Staphylococcus aureus, however, the inhibition zone was narrow with a mean diameter of 20% was 6.8 mm, 40% was 7.4 mm, 60% was 8.2 mm, and 80% was 8.1 mm. It can be concluded that ethanolic extract of binahong leaf had comparative inhibition activity toward Staphylococcus aureus.
Co-Authors Abdul Harris divina dinda hayati Erina Erina Fakhrurrazi Fakhrurrazi Hennivanda Hennivanda Herrialfian . M. Isa Maryulia Dewi Mizla Maulia Nisya Lubis