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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences The Journal of Experimental Life Sciences (JELS) Jurnal Mahasiswa Jurusan Teknik Sipil Jurnal Sains dan Kesehatan Darussalam Journal Of Carbazon
irfan mustafa
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience

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DEGRADATION OF PETROLEUM REFINERY WASTE BY A CONSORTIUM OF HYDROCARBONOCLASTIC BACTERIA ON SEVERAL C:N:P RATIO Syukria I Zam; irfan mustafa
Journal of Tropical Life Science Vol. 2 No. 1 (2012)
Publisher : Journal of Tropical Life Science

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Abstract

Bioremediation is an alternative method to treat petroleum waste using microorganism into nontoxic end product. The method is relatively cheap, effective, and environmental friendly. A key factor influencing bioremediation process for petroleum refinery waste treatment is C:N:P ratio of bacterial growth medium. The objective of this research was to obtain C:N:P ratio of Stone Mineral Salt Solution (SMSS) medium that allow optimal degradation of petroleum refinery waste by consortium of hydrocarbonoclastic bacteria. C:N:P ratio of SMSS medium was adjusted to ratio of 100:10:1, 100:10:0.5, 100:5:1, and 100:5:0.5. We demonstrate that optimal degradation of petroleum refinery waste by a consortium of hydrocarbonoclastic bacteria was achieved in SMSS medium with C:N:P ratio of 100:5:1. It allowed 66.55% degradation of total petroleum hydrocarbon (TPH) and 85.18 % decrease of chemical oxygen demand (COD) value.
The Diversity of Endophytic Bacteria from the Traditional Medicinal Plants Leaves that Have Anti-Phytopathogens Activity Syukria Ikhsan Zam; Anthoni Agustien; Syamsuardi Syamsuardi; Akmal Djamaan; Irfan Mustafa
Journal of Tropical Life Science Vol. 9 No. 1 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.09.01.8

Abstract

Endophytic bacteria live in plant tissues which utilized in plant protection against phytopathogens. This study aims to investigate the diversity of endophytic bacteria from the leaves of traditional medicinal plants that has anti-phytopathogens properties. Isolation of endophytic bacteria was done by spread plate method. The bacteria were characterised by Gram staining and the 16S rRNA gene analysis. Further screening of anti-phytopathogen activity used disc diffusion method for Ralstonia solanacearum, Xanthomonas campestris, Fusarium oxysporum, and Sclerotium rolfsii. All togethers, sixteen isolates of endophytic bacteria from the leaves of eight medicinal plants species were obtained. Fourteen isolates had an anti-phytopathogen (with eight isolates against R. solanacearum, seven isolates against X. campestris, nine isolates against F. oxysporum, and five isolates against S. rolfsii). From the 14 isolates identified, phylum Firmicutes were dominant (64.3%), followed by Proteobacteria (28.6%), and Actinobacteria (7.1%). Phylum Firmicutes consists of Bacillus indicus (BJF1, TCF1, and MCF2), Bacillus pumilus (CAF4), Bacillus sp. (CAF1), Bacillus subtilis (AAF2, MCF1, CAF3, and MCF3); phylum Proteobacteria consists of Pantoea agglomerans (CAF2), Pantoea stewartii (AAF4), Pseudomonas oryzihabitans (AAF3), and Pseudomonas psychrotolerans (AAF1); and phylum Actinobacteria consists of Kocuria kristinae (CSF1).
Isolation and Identification of Nitrogen-Fixing Rhizobacteria associated with Cocoa plantation (Theobroma cacao L) as Biofertilizer Agent Nurul Afiyatul Jannah; Irfan Mustafa; Yoga Dwi Jatmiko
The Journal of Experimental Life Science Vol. 12 No. 3 (2022)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2022.012.03.01

Abstract

Ringinkembar Village, Sumbermanjing Wetan District, Malang Regency, is one of the centers for cocoa plantations using an organic farming system. However, over time this organic farming system experienced a decrease in fruit production, possibly from soil fertility and biofertilizers that were less available in the soil. This study aims to analyze the nitrogen-fixing ability and identify rhizosphere isolates that excel in nitrogen-fixing obtained from the rhizosphere of cacao (Theobroma cacao L) plant. Bacteria were isolated from the soil surrounding cocoa plant roots and grown on Nfb (Nitrogen free Bromothymol Blue) agar media. The nitrogen-fixing bacteria were analyzed with quantitative and qualitative methods. Six potential nitrogen-fixing isolates were identified based on the 16S rDNA sequence. The total number of isolates obtained from nitrogen-fixing isolation was six isolates. The R3.FN1 isolate showed the highest ammonia index at 0.52 µg.L-1 and was identified as Stenotrophomonas maltophilia KB13 with 99.87% similarity to the 16S rDNA sequence. Keywords: Cocoa, Nitrogen-fixing, Rhizosphere, Stenotrophomonas maltophilia.
PENGARUH BAKTERI Bacillus subtilis YANG DIINOKULASI PADA AGREGAT DAUR ULANG TERHADAP KUAT TEKAN DAN MODULUS ELASTISITAS BETON Ervando Dian Prakosa; Eva Arifi; Irfan Mustafa
Jurnal Mahasiswa Jurusan Teknik Sipil Vol. 1 No. 3 (2023)
Publisher : Jurusan Teknik Sipil, Fakultas Teknik, Universitas Brawijaya

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Abstract

Pesatnya pembangunan infrastruktur menjadi faktor peningkatan kebutuhan bahan konstruksi, yaitu beton. Penggunaan beton menyebabkan turunnya jumlah sumber daya alam serta mengakibatkan adanya limbah beton pada lingkungan. Salah satu alternatif yang dapat dilakukan ialah dengan pemanfaatan agregat kasar daur ulang/recycled coarse aggregate (RCA) sebagai pengganti dari agregat kasar alami/natural coarse aggregate (NCA). Namun, RCA mempunyai kekuatan yang lebih rendah dibandingkan NCA, sehingga diperlukan upaya perbaikan untuk peningkatan kualitas RCA. Upaya perbaikan yang dilakukan ialah penggunaan bakteri pengendap kalsium karbonat (CaCO3) pada RCA karena dianggap ramah lingkungan dan ekonomis dalam pengaplikasiannya. Metode perbaikan yang dilakukan menggunakan isolat bakteri Bacillus subtilis R20 dengan densitas sel dalam medium sebanyak 105 sel/ml. Perbaikan RCA dilakukan dengan proses inkubasi dengan bakteri Bacillus subtilis R20 selama 14 hari. Selanjutnya dilakukan pembuatan benda uji beton silinder berukuran tinggi 30 cm dengan diameter 15 cm. Pengujian benda uji dilakukan ketika umur beton mencapai 28 hari dengan pengujian kuat tekan dan modulus elastisitas. Penggunaan bakteri pengendap kalsium karbonat (CaCO3) pada RCA dapat meningkatkan kualitas agregat dibandingkan dengan RCA tanpa adanya perbaikan dengan meningkatnya berat isi sebesar 1,92% pada kondisi agregat jenuh kering permukaan dan sebesar 1,70% pada kondisi agregat kering oven, berat jenis sebesar 0,41%, serta menurunnya kadar air sebesar 57%. Dengan peningkatan kualitas material RCA, tentunya berpengaruh dalam peningkatan kuat tekan dan modulus elastisitas beton. Namun karena adanya faktor lain yang berpengaruh dalam pembuatan benda uji menyebabkan kuat tekan dan modulus elastisitas beton RCA bakteri tidak maksimal dibanding beton RCA. Kata kunci: agregat kasar daur ulang, bakteri pengendap kalsium karbonat, beton, kuat tekan, modulus elastisitas.
PEMBUATAN KERTAS INDIKATOR ALAMI DARI EKSTRAK BUAH JAMBLANG SEBAGAI PENENTU SIFAT ASAM DAN BASA SUATU LARUTAN: PEMBUATAN KERTAS INDIKATOR ALAMI DARI EKSTRAK BUAH JAMBLANG SEBAGAI PENENTU SIFAT ASAM DAN BASA SUATU LARUTAN Azmalina Adriani; Arina Ahsana; Irma Zarwinda; Irfan Mustafa
Journal of Science and Health Darussalam Vol. 3 No. 1 (2023): Jurnal Sains dan Kesehatan Darussalam
Publisher : Akademi Analis Farmasi dan Makanan Banda Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56690/jskd.v3i1.79

Abstract

Buah Jamblang merupakan buah yang memiliki warna ungu tua, warna ungu pada buah disebabkan oleh antosianin, antosianin merupakan pembentuk pigmen warna merah, ungu dan biru pada tanaman yang dapat dimanfaatkan sebagai indikator alami, yang dapat menggantikan indikator sintesis seperti Methyl orange, Phenolthalein dan Metil merah. Penelitian ini bertujuan untuk membuat kertas indikator asam basa dari ekstrak buah jamblang sebagai pengganti indikator lakmus. Metodelogi penelitian ini adalah Eksperimental Laboratorium. Penelitian dilakukan di Laboratorium Kimia Akafarma Banda Aceh pada bulan Mei 2022. Pengambilan sampel secara total sampling dengan ekstrak buah jamblang yang diperoleh dengan cara maserasi menggunakan etanol 96% sebagai pelarut. Indikator dibuat dari kertas saring yang dicelupkan dalam ekstrak buah jamblang dan dikeringkan. Dilakukan pengujian ekstrak buah jamblang pada sampel jeruk nipis, sprite, cuka, shampo, detergen dan baking soda. Dari hasil diperoleh kertas indikator ekstrak buah jamlang mengalami perubahan warna seperti sprite merah keungguan, jeruk nipis merah, cuka unggu, shampo biru, detergen unggu kebiruan serta baking soda berwarna kuning kehijauan. Berdasarkan hasil penelitian dapat disimpulkan bahwa kertas indikator dari ekstrak buah jamblang dapat digunakan sebagai kertas indikator alami dalam penentuan asam basa.
Polyurethane Membranes From Red Seaweeds For Ammonia Adsorption Nurul Kamila; Marlina Marlina; Irfan Mustafa; Salfauqi Nurman; Saiful Saiful
Journal of Carbazon Vol 1, No 1 (2023): June 2023
Publisher : University of Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jokarbazon.v1i1.32874

Abstract

Polyurethane (PU) membranes were prepared from red seaweed (Gracilaria sp.), castor oil, Toluene Diisocyanate (TDI), and added benzoyl peroxide (BPO) additive to improve membrane performance. The membrane is applied for the adsorption of ammonia in the solution. FT-IR analysis showed the presence of functional group N = C = O at a wavenumber of 2276 cm-1 and the O-H functional group at a wave number of 3373.50 cm-1, indicating the urethane group's formation has been formed during membrane polymerization. SEM images showed the morphological structure of the PU membrane, where the more open structure of the PU membrane by the addition of BPO. The DSC and TGA results showed the membrane's thermal stability with the addition of BPO. The PU membrane has an optimum contact time for ammonia uptake of 40 minutes. The adsorption isotherm of ammonia by the PU membrane follows the Freundlich isotherm model. The adsorption capacity of the PU membrane with the addition of BPO additives was 13.2 mg/g,  which shows that the adsorption capacity of the PU-BPO membrane has a better performance than a membrane without the addition of the BPO. The PU-BPO membrane could be used as an alternative method of ammonia removal.
The Effect of Antioxidants of α-Tocopherol on the Characteristic of Mechanical, Thermal, and Morphology Properties of Polypropylene-Montmorillonite (PP-MMT) Nanocomposites Julinawati Julinawati; Rahmi Rahmi; Kartika MZ.; Irfan Mustafa; Suryati Suryati
Journal of Carbazon Vol 1, No 1 (2023): June 2023
Publisher : University of Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jokarbazon.v1i1.32812

Abstract

The purpose of this study was to determine the effect of α-tocopherol on the mechanical, thermal, and morphological properties of polypropylene-montmorillonite (PP-MMT) nanocomposites. The preparation of polypropylene-montmorillonite (PP-MMT-AO) nanocomposites was carried out on PP compositions; PP-g-MA; MMT-octadecyl amine; AO is 80;10;10;5 and the PP-MMT nanocomposite composition is PP; PP-g-MA; MMT-Octadecyl amine: 80;10;10. The SEM results showed that polypropylene, MMT, and the antioxidant α-tocopherol could be exfoliated and intercalated to produce compatible nanocomposites. The mechanical and thermal test results showed that the PP-MMT-AO nanocomposite had higher mechanical properties and thermal stability than the PP-MMT nanocomposite. Based on the FTIR test and degradation test on PP-MMT and PP-MMT-AO films which were carried out for up to 15 days and 30 days showed that the PP-MMT-AO nanocomposite against UV light was given, this shows that α-Tocopherol can prevent oxidation reactions polypropylene.
Co-Authors ., Julinawati Adriani, Azmalina Akmal Djamaan Anthoni Agustien Arina Ahsana Ervando Dian Prakosa Eva Arifi Kartika MZ. Marlina Marlina Nurul Afiyatul Jannah Nurul Kamila Rahmi Rahmi Saiful Saiful Salfauqi Nurman, Salfauqi Suryati Suryati Syamsuardi Syamsuardi Syukria I Zam Syukria Ikhsan Zam Yoga Dwi Jatmiko Zarwinda, Irma