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Genetic relationships of Kampung, Pelung, Sentul and Black Kedu Chicken using Microsatellite DNA Markers: I. Lingkage group of macro chromosome Sartika, Tike; Iskandar, S; Prasetyo, L.H; Takahashi, H; Mitsuru, M
Indonesian Journal of Animal and Veterinary Sciences Vol 9, No 2 (2004)
Publisher : Indonesian Animal Sciences Society

Genetic relationships of Kampung, Pelung, Sentul and Black Kedu chickens were studied on the basis of microsatellite DNA polymorphism. DNA samples were analyzed using nine microsatellite markers which chosen from linkage group of macrochromosome (chromosome 1-8) such as, locus ABR 258, ABR359, ABR 297, ABR 339, ABR 75, ABR 209, ABR 28, ABR 419 and ABR 604. Analyses of amplified DNA fragments were performed using Gene Mapper 2.0 software (PE, Applied Biosystems). The allele frequencies in each breed estimated by direct counting. Since all nine microsatellite markers were polymorphic, genetic distance between the breeds could be calculated based on the frequencies of alleles of the microsatellite. Genetic relationships between the breeds could be constructed. The results indicated that a total of 73 allele were detected while typing all the four breeds of local chicken and one breed of White Leghorn as outgroup breed acrossed nine loci. The number of alleles was observed in all of the breed ranged 3-17 alleles according to the microsatellite under scrutiny. Highest observed number of alleles was found in Kampung Chicken 60 alleles (82.2%). The UPGMA method for dendogram based on Nei genetic distances indicated that the local chickens have the same of ancestor, while Kampung and Sentul chicken have the same cluster followed by Black Kedu and Pelung Chicken. Â Key words: Local chicken, microsatellite, macrochromosome, genetic distance

Comparison of external genetic of Wareng and Kampung Chicken, observed from introgression rate and genetic variability Sartika, T; Wati, D.K; Iman Rahayu, H.S; Iskandar, S
Indonesian Journal of Animal and Veterinary Sciences Vol 13, No 4 (2008)
Publisher : Indonesian Animal Sciences Society

Wareng and Kampung chicken are Indonesian native chicken that have good potential to be dual purpose chicken. Information on these chickens has not hastân widely published so that their genetic potential is unknown. The purpose of thisÂ research is to collect basic data of the external genetic characteristic from Wareng and Kampung chickens consisting feather color, feather pattern, feather feature, feather shine, shank color and comb shape; to identify rate of introgression imported breed (Rhode Island Red, White Leghorn and Barred Plymouth Rock), the purity and genetic variability of Wareng and Kampung chickens. This study was carried out at the Research Institute for Animal Production, Ciawi, Bogor. Materials used were 361 of Wareng chickens (313 females, 48 males) and 439 of Kampung chickens (352 females, 87 males). Data were analyzed using formulas to identify gene frequency, rate of introgression of purity native gene frequency and the genetic variability. The result showed that the control of gene constitution on external characteristic of Wareng chicken was I_ E_ bb S_ Id_ pp and ii e+ _bb ss idid pp on Kampung chicken. Wareng chicken own constitution of gene the same as with White Leghorn (II EE SS BB IdId pp). Wareng Chicken is not containing frequency of original gene of Indonesian local chicken (Kampung). The level of influence value (rate of introgression) from Europe and American chicken for Wareng chicken was of equal to 84% and 25% to Kampung chicken. So that the purity for Wareng chicken was 16% and 75% was for Kampung chicken. The variability genetic of Kampung chickens (39%) higher than Wareng chicken (16%). Â Â Key Words: Wareng Chicken, Kampung Chicken, External Genetic, Introgression Rate

The use of kemiri seed meal (Aleurites mollucana Willd.) in native chickens diet Resnawati, Hesti; Iskandar, S; ., Surayah
Indonesian Journal of Animal and Veterinary Sciences Vol 3, No 3 (1998)
Publisher : Indonesian Animal Sciences Society

An experiment was carried out to evaluate the optimum level of kemiri seed meal for native chickens. One hundred unsexed day-old native chickens were reared in 20 cages with five birds per cage. Five dietary treatments were formulated to contain different levels of kemiri seed meal: 0% (R0), 5% (R5), 10% (R10), 15% (R15) and 20% (R20). All diets were formulated to be isoprotein and isoenergy with 14% crude protein and 2,850 kcal ME/ kg diet. The experiment was arrangedin a completely randomized design with five treatments and four replicates. Feed consumption, body weight gain and intestinal weight were significantly (P<0.01) affected by inclusion of kemiri seed meal in the diet. But the feed conversion, carcass weight and liver weight were not affected significantly. It was concluded that kemiri seed meal can be added until 5% in the diet of growing native chickens. Â Key words : Native chickens, kemiri seed meal, growth

Local Duck Fanning At Paddy Three Times Planting Areas ("IP Padi 300") A.R, Setioko; Iskandar, S; Raharjo, Y.C; Soedjana, T.D; Murtisari, T; Purba, M; Estuninngsih, S; Sunandar, N; Sarosa, D
Indonesian Journal of Animal and Veterinary Sciences Vol 5, No 1 (2000)
Publisher : Indonesian Animal Sciences Society

Most of duck husbandry in Indonesia is still run traditionally, herded in rice field or in the swampy area. This kind of husbandry seemed to be much preferred by farmers as they thought it was a simple and did not need high skill and high capital "IP padi 300" was a term of rice planting system tree times instead of twice in a year. This kind of changing might have significantly affected duck faming. The objective of the study was to observe the interactively effect of "IP padi 300" to duck husbandry at the same area. Two locations were choosen (Subang, West Jawa and Pemalang, Central Java) with 5 farmers at each location to be involved in the study. As many as 1200 laying pullet ducks were distributed to 10 farmers at two locations. The farmers were suggested to raise laying ducks with their own systems (fully intensive, semi intensive and fully herded) and were observed for 6 months. Biota was observed on both field and in the crop of the laying ducks. There was an interactive effect of "IP padi 300" and the duck farming on the same area. The availability of feed was increased on the "IP padi 300", which gave benefit to duck farming especially fue herded system, not to go far from owners home base. Whilst the benefit to "IP padi 300" was assumed to the reduction of pest and desease, which was frequently attacked the rice field. The production of egg from herded duck was very fluctuative due to the movement and feed availability in the rice field. Field biota in Subang and Pemalang was very much the same in profile, although "golden snail" was only found in Subang. Ducks crop content seemed to be very much similar with the profile of field biota, although rice grain was the most in the crop. Thus, it was found that field biota was not reduced by "IP padi 300", in fact it was rather increased. Â Key words: IP 300, herded ducks, duck farming

Laying Performance of Wareng Chicken under Free Choice Feeding and Different Cage Density S Iskandar
Media Peternakan Vol. 34 No. 1 (2011): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

The aim of the experiment was to explore the possibility of using free choice feeding technique under different cage density for unselected native chicken. Eighty hens of laying Wareng chicken were divided into two feeding systems. Half of them were fed complete commercial diet of 17% crude protein, with 2800 kcal ME/kg and the other half were subjected to free choice diet. The diets were in mash form and placed in sufficient feed troughs in front of each cage. Each cage was occupied by four or six hens. Free choice feeding hens were served with commercial concentrate (30% crude protein), ground corn and oyster shell, placed separately in feed troughs. Observation was carried out during 24 weeks laying period, starting from 20 to 44 weeks of age. The results showed that egg production (9.35% hen day), and the intake of feed (42.74 g/day), protein (7.01 g/day), energy (116.6 kcal ME/day), calcium (1.99 g/day and phosphorus (0.22 g/day) were not affected (P>0.05) by feeding system nor by cage density. The results however indicated that free choice feeding technique provided sufficient nutrients in supporting maximum egg production of unselected native chicken.

Genetic relationships of Kampung, Pelung, Sentul and Black Kedu Chicken using Microsatellite DNA Markers: I. Lingkage group of macro chromosome Tike Sartika; S Iskandar; L.H Prasetyo; H Takahashi; M Mitsuru
Jurnal Ilmu Ternak dan Veteriner Vol 9, No 2 (2004): JUNE 2004
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Genetic relationships of Kampung, Pelung, Sentul and Black Kedu chickens were studied on the basis of microsatellite DNA polymorphism. DNA samples were analyzed using nine microsatellite markers which chosen from linkage group of macrochromosome (chromosome 1-8) such as, locus ABR 258, ABR359, ABR 297, ABR 339, ABR 75, ABR 209, ABR 28, ABR 419 and ABR 604. Analyses of amplified DNA fragments were performed using Gene Mapper 2.0 software (PE, Applied Biosystems). The allele frequencies in each breed estimated by direct counting. Since all nine microsatellite markers were polymorphic, genetic distance between the breeds could be calculated based on the frequencies of alleles of the microsatellite. Genetic relationships between the breeds could be constructed. The results indicated that a total of 73 allele were detected while typing all the four breeds of local chicken and one breed of White Leghorn as outgroup breed acrossed nine loci. The number of alleles was observed in all of the breed ranged 3-17 alleles according to the microsatellite under scrutiny. Highest observed number of alleles was found in Kampung Chicken 60 alleles (82.2%). The UPGMA method for dendogram based on Nei genetic distances indicated that the local chickens have the same of ancestor, while Kampung and Sentul chicken have the same cluster followed by Black Kedu and Pelung Chicken. Key words: Local chicken, microsatellite, macrochromosome, genetic distance

The use of kemiri seed meal (Aleurites mollucana Willd.) in native chickens diet Hesti Resnawati; S Iskandar; Surayah .
Jurnal Ilmu Ternak dan Veteriner Vol 3, No 3 (1998)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

An experiment was carried out to evaluate the optimum level of kemiri seed meal for native chickens. One hundred unsexed day-old native chickens were reared in 20 cages with five birds per cage. Five dietary treatments were formulated to contain different levels of kemiri seed meal: 0% (R0), 5% (R5), 10% (R10), 15% (R15) and 20% (R20). All diets were formulated to be isoprotein and isoenergy with 14% crude protein and 2,850 kcal ME/ kg diet. The experiment was arrangedin a completely randomized design with five treatments and four replicates. Feed consumption, body weight gain and intestinal weight were significantly (P<0.01) affected by inclusion of kemiri seed meal in the diet. But the feed conversion, carcass weight and liver weight were not affected significantly. It was concluded that kemiri seed meal can be added until 5% in the diet of growing native chickens. Key words : Native chickens, kemiri seed meal, growth

Local Duck Fanning At Paddy Three Times Planting Areas ("IP Padi 300") Setioko A.R; S Iskandar; Y.C Raharjo; T.D Soedjana; T Murtisari; M Purba; S Estuninngsih; N Sunandar; D Sarosa
Jurnal Ilmu Ternak dan Veteriner Vol 5, No 1 (2000): MARCH 2000
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Most of duck husbandry in Indonesia is still run traditionally, herded in rice field or in the swampy area. This kind of husbandry seemed to be much preferred by farmers as they thought it was a simple and did not need high skill and high capital "IP padi 300" was a term of rice planting system tree times instead of twice in a year. This kind of changing might have significantly affected duck faming. The objective of the study was to observe the interactively effect of "IP padi 300" to duck husbandry at the same area. Two locations were choosen (Subang, West Jawa and Pemalang, Central Java) with 5 farmers at each location to be involved in the study. As many as 1200 laying pullet ducks were distributed to 10 farmers at two locations. The farmers were suggested to raise laying ducks with their own systems (fully intensive, semi intensive and fully herded) and were observed for 6 months. Biota was observed on both field and in the crop of the laying ducks. There was an interactive effect of "IP padi 300" and the duck farming on the same area. The availability of feed was increased on the "IP padi 300", which gave benefit to duck farming especially fue herded system, not to go far from owner's home base. Whilst the benefit to "IP padi 300" was assumed to the reduction of pest and desease, which was frequently attacked the rice field. The production of egg from herded duck was very fluctuative due to the movement and feed availability in the rice field. Field biota in Subang and Pemalang was very much the same in profile, although "golden snail" was only found in Subang. Ducks' crop content seemed to be very much similar with the profile of field biota, although rice grain was the most in the crop. Thus, it was found that field biota was not reduced by "IP padi 300", in fact it was rather increased. Key words: IP 300, herded ducks, duck farming

Comparison of external genetic of Wareng and Kampung Chicken, observed from introgression rate and genetic variability T Sartika; D.K Wati; H.S Iman Rahayu; S Iskandar
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 4 (2008): DECEMBER 2008
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Wareng and Kampung chicken are Indonesian native chicken that have good potential to be dual purpose chicken. Information on these chickens has not hast’n widely published so that their genetic potential is unknown. The purpose of this research is to collect basic data of the external genetic characteristic from Wareng and Kampung chickens consisting feather color, feather pattern, feather feature, feather shine, shank color and comb shape; to identify rate of introgression imported breed (Rhode Island Red, White Leghorn and Barred Plymouth Rock), the purity and genetic variability of Wareng and Kampung chickens. This study was carried out at the Research Institute for Animal Production, Ciawi, Bogor. Materials used were 361 of Wareng chickens (313 females, 48 males) and 439 of Kampung chickens (352 females, 87 males). Data were analyzed using formulas to identify gene frequency, rate of introgression of purity native gene frequency and the genetic variability. The result showed that the control of gene constitution on external characteristic of Wareng chicken was I_ E_ bb S_ Id_ pp and ii e+ _bb ss idid pp on Kampung chicken. Wareng chicken own constitution of gene the same as with White Leghorn (II EE SS BB IdId pp). Wareng Chicken is not containing frequency of original gene of Indonesian local chicken (Kampung). The level of influence value (rate of introgression) from Europe and American chicken for Wareng chicken was of equal to 84% and 25% to Kampung chicken. So that the purity for Wareng chicken was 16% and 75% was for Kampung chicken. The variability genetic of Kampung chickens (39%) higher than Wareng chicken (16%). Key Words: Wareng Chicken, Kampung Chicken, External Genetic, Introgression Rate

Penilaian performans ayam lokal leher gundul Sampai umur 12 minggu Devi Yuliananda; R Kartasudjana; S Iskandar
Kandang : Jurnal Peternakan Vol 6 No 1 (2014)
Publisher : Prodi Ilmu Peternakan Universitas Muhammadiyah Cirebon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32534/jkd.v6i1.203

AbstrakDalam rangka mempelajari dan menggkaji tentang performans ayam lokal leher gundul ditinjau dari aspek energetik, lima puluh ekor anak ayam leher gundul dan lima puluh ekor anak ayam berbulu lengkap digunakan dalam kajian terhadap pengaruh kadar energi ransum dan fenotip ayam pada variabel-variabel produksi (konsumsi pakan, pertambahan bobot badan, konversi ransum, bobot karkas). Dua kadar energi ransum (3098, 2 kkal me/kg dan 2655,8 kkal me/kg) serta dua fenotip ayam (leher gundul dan berbulu lengkap) digunakan sebagai faktor dalam rancangan penelitian (ral). Setiap kombinasi perlakuan dalam penelitian ini diulang sebanyak lima kali, dan setiap unit percobaan terdiri dari lima ekor ayam. Ayam ditempatkan pada kandang petak dengan alas kawat. Ransum diberikan ad-libitum dengan kandungan protein ransum Â± 16,5%. Hasil penelitian menunjukkan bahwa performans produksi ayam lokal tidak dipengaruhi oleh fenotip maupun kadar energi ransum (pËƒ0,05) dalam hal pertumbuhan, konversi ransum, dan produksi karkas, tetapi fenotip berpengaruh nyata terhadap konsumsi ransum (pË‚0,05) yang ditunjukkan oleh rataan konsumsi ransum kumulatif pada ayam leher gundul lebih banyak daripada ayam berbulu lengkap (4.922,47 vsÂ 4.673,08 g/ekor). Berdasarkan hasil yang diperoleh dari penelitian ini, dapat disimpulkan bahwa performans ayam lokal leher gundul akibat pengaruh kadar energi ransum tidak berbeda dengan respon pertperformans ayam berbulu lengkap. Akan tetapi, pemberian ransum dengan kandungan energi rendah (2655,82 kkal me/kg) memberikan respon positif terhadap peningkatan efisiensi pemanfaatan energi ransum.Kata kunci: ayam lokal leher gundul, performans, umur 12 minggu

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