Garuda - Garba Rujukan Digital

p-Index From 2019 - 2024

0.23

P-Index

This Author published in this journals

All Journal Tropical Medicine Journal INDONESIAN JOURNAL OF PHARMACY Jurnal Kimia Terapan Indonesia PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) JURNAL ILMU KEFARMASIAN INDONESIA Media Farmasi Indonesia

Ratna Asmah Susidarti

Unknown Affiliation

Author-ID : 2892967

Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Other

Published : 6 Documents Claim Missing Document

Claim Missing Document

Articles

In vivo Antiplasmodial of the Most Active Fraction and Its Compound of Kapur Leaves (Harmsiopanax aculeatus Harms) Extract Against Plasmodium berghei Rachel Turalely; Ratna Asmah Susidarti; Mahardika Agus Wijayanti
Tropical Medicine Journal Vol 1, No 2 (2011): Tropical Medicine Journal
Publisher : Pusat Kedokteran Tropis

Introduction : The rising of Plasmodium resistance towards chloroquine and other antimalarial drugs have encouraged to discover and develop new drugs mainly derived from natural products. Harmsiopanax aculeatus (kapur plant) has traditionally used by people of in Maluku Province to treat malaria.Objectives: The aims of this study were to identify antiplasmodial activity and its chemical constituents of the most active fraction of kapur leaves.Methods: The dried powder of Kapur leaves (1.3 kg) were extracted successively by maceration with n-hexane, ethyl acetate and methanol. After removal the solvents the hexane 15.6 g (1.2%), ethyl acetate 53.3 g (4.1%) and methanol 61.1 g (4.7%) extracts were obtained. Those extracts were assayed for their in vivo antiplasmodial activities by using 4-days suppressive test in Swiss mice infected with Plasmodium berghei, HPIA and identified the compound by GC-MS.Results: The ED50 of hexane, ethyl acetate and methanol extracts were 467.58, 2074.02 and 16.16 mg/kgBW, respectively. Fractionation of the methanol extract gave 18 combined fractions (FG1 – FG18). FG8 was the most active fraction with the IC50 HPIA of 18.22 μg/ml. Phytochemical test of this fraction using spray reagent showed the existence of essential oils, triterpenoids, and phenolic compounds. Separation of FG8using pressed chromatography gave 19 combined fractions (FG8.1-FG8.19). The fraction containing intense blue fluorescent spot (FG8.5) was further separated by PLC fourthly eluted with chloroform. Seven major components with the percentage of compotition more than 3.11% were identified as eugenol (tr = 12.692; 18.22%), isoprophyl myristate (tr = 16.333; 3.99%); bis(2-methylpropyl) phtalat (tr = 16.939; 7.15%); methyl palmitic (tr = 17.442; 3.11%); palmitic acid (tr = 17.883; 25.72%); butyl 2-methylpropyl phtalat (tr = 17.957; 9.37%) and bis(2-ethylhexyl) phtalat (tr = 23.258; 23%).Conclusion: Methanol extract of H. aculeatus was the most potential in vivo antiplasmodial activity. Combined fraction 8 which contain 7 compounds was the most active fraction.Keywords: Harmsiopanax aculeatus Harms, in vivo antiplasmodial, HPIA, PLC, GC-MS

SYNTHESIS AND CYTOTOXIC ACTIVITY OF 2,5-BIS(4-BORONIC ACID)BENZYLIDINE CYCLOPENTANONE ON HER2 OVEREXPRESSED-CANCER CELLS Rohmad Yudi Utomo; Herwandhani Putri; Pudjono Pudjono; Ratna Asmah Susidarti; Riris Istighfari Jenie; Edy Meiyanto
Indonesian Journal of Pharmacy Vol 28 No 2, 2017
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Development of chemotherapeutic agent and boron carrying pharmaceutical based on HER2 specific targeted is important due to its role in enhancing cancer progression. The purpose of this study is to synthesize curcumin analogue, namely Pentagamaboron-0 (PGB-0) or 2,5-bis(4-boronic acid)benzylidine cyclopentanone, and to explore the cytotoxic activity on HER2 overexpressed-cancer cells. MCF-7/HER2 was used as a model of HER2 overexpressed-cancer cells and NIH3T3 as normal cells. PGB-0 bound to ATP binding site of HER2 and EGFR based on molecular docking study. PGB-0 was synthesized resulting in 33% yield and was confirmed by IR, 1HNMR, 13CNMR and Mass spectroscopy. Based on MTT assay PGB-0 decreased cells viability on MCF-7/HER2 cells with IC50 value of 270 µM but performed no effect on NIH3T3 cells. Cell cycle analysis revealed that PGB-0 increased sub-G1 accumulation. PGB-0 decreased HER2 expression in a dose-dependent manner. We conclude that the new compound PGB-0 inhibits cell growth through cell death induction and decreased HER2 expression. Thus, PGB-0 is potential to be developed as a chemotherapeutic agent and boron carrying pharmaceutical targeted on the HER2 receptor.

Mengungkap Potensi Kluwak (Pangium edule Reinw) Sebagai Antioksidan Alami dan Penghambat Senescence Ratih Kurnia Wardani; Yurananda Magnalia Putri; Dhella Angelina Nurjanah; Ratna Asmah Susidarti
JURNAL ILMU KEFARMASIAN INDONESIA Vol 19 No 2 (2021): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35814/jifi.v19i2.958

Senescence is a condition that causes tissue dysfunction, causing problems such as premature senescence. Premature senescence is caused by oxidative stress that comes from reactions in the body and the environment (UV rays, X rays, and free radicals). Kluwak seeds (Pangium edule Reinw) contain a variety of powerful antioxidant compounds such as vitamin E, vitamin C, polyphenols, flavonoids (quercetin and catechins), quinine, and β carotene. This narrative review aims to uncover kluwak’s potential as a natural senescence-inhibiting agent for antioxidants. The literature search method with the Booelan technique is used by combining several keywords with the notation AND, OR, NOT from various literatures from national and international journals published from various databases. The results showed that the compounds in kluwak can prevent oxidative stress that causes senescence because they have antioxidant activity from phenolic groups and conjugated double bonds. The mechanisms possessed by kluwak seeds are: stabilizing ROS, increasing antioxidant enzymes in the body, inhibiting prooxidant enzymes, preventing lipid peroxidation, and inhibit markers of senescence (anti-SASP). Therefore, kluwak is used as an anti-senescence agent in the form of cosmetic preparations that have local effects, such as hydrogels which are easy and safe to use in the body.

TOKSISITAS AKUT EKSTRAK ETANOLIK BIJI BUAH PINANG (Areca catechu L.) TERHADAP TIKUS JANTAN GALUR SPRAGUE DAWLEY Sri Handayani; Edy Meiyanto; Riris Istighfari Jenie; Ratna Asmah Susidarti
Jurnal Kimia Terapan Indonesia Vol 14, No 2 (2012)
Publisher : Research Center for Chemistry - LIPI

Ekstrak etanolik biji buah pinang (Areca catechu L.) memperlihatkan aktivitas penghambatan proliferasi selkanker payudara T47D dan MCF-7. Tikus (Rattus norvegicus) jantan galur Sprague Dawley usia 8 minggu dibagidalam lima kelompok, yaitu empat kelompok perlakuan dan satu kelompok kontrol pelarut CMC-Na 5%. Pemejanandosis tunggal peroral dilakukan terhadap subjek uji dengan variasi dosis ekstrak etanolik biji pinang mulai dari dosisyang paling aman hingga dosis tertinggi yang diharapkan toksik pada semua hewan uji (0,1; 0,72; 5,36 dan 10gram/kg berat badan). Pengamatan dilakukan selama 24 jam terhadap gejala-gejala toksik, wujud dan mekanismeefek toksik maupun patologi organ vital. Pengamatan mikroskopis berupa histopatologi beberapa organ vitaldilakukan dengan pengecatan Hematoxillen&Eosin (H&E). Hasil uji menunjukan bahwa semua kelompok dosisperlakuan tidak mengalami gejala toksik, sama halnya dengan kelompok kontrol. Pemberian dosis tunggal ekstraketanolik biji pinang tidak menimbulkan kematian pada subjek uji, bahkan pada dosis tertinggi sekalipun. Oleh karenaitu pengamatan dilanjutkan hingga 14 hari. Pada hari ke-15 dilakukan pembedahan pada semua subjek uji.Pengamatan mikroskopis menunjukkan tidak ada perubahan histopatologis yang berarti pada seluruh kelompokperlakuan. Hasil uji ini menambah dasar keamanan penggunaan ekstrak etanolik biji pinang dalam pengembangannyasebagai agen terapi alternatif. Kata kunci: Ekstrak etanolik, Areca catechu, toksisitas akut, tikus

Optimasi Sintesis Senyawa 1-(2,5-Dihidroksifenil)-(3-Piridin-2-IL) Propenon Sebagai Antiinflamasi Menggunakan Variasi Katalis NaOH Andy Eko Wibowo; Andy Kurniawan Saputra; Ratna Asmah Susidarti
PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) Jurnal Pharmacy, Vol. 15 No. 02 Desember 2018
Publisher : Pharmacy Faculty, Universitas Muhammadiyah Purwokerto

Senyawa 1-(2,5-dihidroksifenil)-(3-piridin-2-il)-propenon merupakan senyawa kalkon yang memiliki aktifitas antiinflamasi sebanding dengan ibuprofen dan aktifitas antioksidannya sangat kuat setara dengan quercetin. Senyawa ini telah disintesis menggunakan 2,5-dihidroksiasetofenon dan piridin-2-karbaldehida dengan metode radiasi microwave dan katalis K2CO3 tanpa pelarut selama 4 menit. Dalam upaya memperoleh rendemen yang lebih baik, dilakukan penelitian dengan mengganti katalis, yaitu menggunakan katalis NaOH. Penelitian dilakukan dengan mereaksikan senyawa 2,5-dihidroksiasetofenon dan piridin-2-karbaldehida dengan variasi katalis NaOH sebesar 0–0,002 mol. Senyawa disintesis menggunakan kekuatan radiasi microwave sebesar 140 watt selama 4 menit. Setelah proses sintesis maka dilakukan perhitungan rendemen senyawa 1-(2,5-dihidroksifenil)-(3-piridin-2-il)-propenon untuk mengetahui massa katalis NaOH yang optimal dalam menghasilkan rendemen terbanyak. Berdasarkan hasil yang didapat, massa katalis optimum untuk sintesis senyawa 1-(2,5-dihidroksifenil)-(3-piridin-2-il)-propenon adalah 0,0010 mol dengan rendemen sebesar 13,23%.

ANALISIS HKSA SENYAWA TURUNAN EURIKUMANON SEBAGAI ANTIMALARIA MENGGUNAKAN PENDEKATAN REGRESI LINIER BERGANDA Hanifah Yusuf; Mustofa; Mahardika Agus Wijayanti; Ratna Asmah Susidarti; Andrian Saputra; Iqmal Tahir
Media Farmasi Indonesia Vol. 8 No. 1 (2013): Media Farmasi Indonesia
Publisher : SEKOLAH TINGGI ILMU FARMASI YAYASAN PHARMASI SEMARANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (431.819 KB)

Quantitative Structure Activity Relationship (HKSA) study of eurycomanone derivatives as antimalarial compound was done using multiple linear regression approaches. HKSA equation was obtained from theoretical descriptors data which involved atomic net charges (q), partition coefficient (log P), molecular volume (VM), molecular surface area (LP), molecular weight (BM), and HOMO-LUMO energy differences (EHOMO-ELUMO) versus minimum concentration to inhibit 50% of parasite growth Plasmodium falciparum (log IC50) growth. Descriptors data were obtained by applying AM1 semiempirical quantum mechanics calculation using Hyperchem 7.5 software, while the regression data processes were runned using SPSS 17.0 program. Based on multiple linear regression approaches, there is a selected quantitative relationship between log IC50 to the descriptors that was shown by HKSA equation below: Log IC50 = – 3048.930 + 5137.957qC2 – 13799.126qC5 – 1537.764qC12 + 556.313qC16 – 817.654qO29 + 4.654log P – 0.146VM + 0.270BM with n =10, r = 0.982, SE = 1.262, Fcalc/Ftable = 17.805, and PRESS = 1.593

Co-Authors Andrian Saputra Andy Eko Wibowo Andy Kurniawan Saputra Dhella Angelina Nurjanah Edy Meiyanto Hanifah Yusuf Herwandhani Putri Iqmal Tahir Mahardika Agus Wijayanti Mustofa Mustofa Pudjono Pudjono Rachel Turalely Ratih Kurnia Wardani Riris Istighfari Jenie Rohmad Yudi Utomo Sri . Handayani Yurananda Magnalia Putri

Title

Found 6 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 6 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 6 Documents
Search