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All Journal Jurnal Sain Veteriner Buletin Peternakan Buletin Veteriner Udayana Jurnal Veteriner JOURNAL OF COASTAL DEVELOPMENT Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati IGKOJEI: Jurnal Pengabdian Masyarakat
Hastari Wuryastuty
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Education Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Pengaruh Pemberian Teri Asin Terhadap Ekskresi Kalsium Urin dan Mineralisasi Tulang Femur Tikus Penderita Hartiningsih .; Irkham Widiyono; Hastari Wuryastuty
Jurnal Sain Veteriner Vol 23, No 2 (2005): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (950.383 KB) | DOI: 10.22146/jsv.472

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Dinamika Folikel Ovulasi Setelah Sinkronisasi Estrus dengan Prostaglandin F2a pada Sapi Perah Prabowo Purwono Putro; R. Wasito; Hastari Wuryastuty; Soedarmanto Indarjulianto
Buletin Peternakan Vol 32, No 3 (2008): Buletin Peternakan Vol. 32 (3) Oktober 2008
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v32i3.1257

Abstract

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Efek Deksametason pada Homeostasis Kalsium dan Ekspresi Transforming Growth Factor Beta-1 Tulang Femur Tikus Wistar Pasca Transplantasi Menggunakan Demineralized Freeze-Dried Bovine Bone Xenograft Luh Made Sudimantini; Dhirgo Adji; Hastari Wuryastuty
Buletin Veteriner Udayana Vol. 12 No. 2 August 2020
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1495.041 KB) | DOI: 10.24843/bulvet.2020.v12.i02.p01

Abstract

This research aims to evaluate the safety of dexamethasone use as an anti-inflammatory and analgesic on the handling of femur fracture of rats by bone graft method. The sample used was 32 male Wistar rats aged 2 months were divided into two groups, 16 rats for each. Group I was fractured rats and fixed with intramedullary pin without bone graft, whereas Group II was fractured and fixed with intramedullary pin and demineralized freeze-dried bovine bone xenograft (DFDBBX). Group I and II were then divided into two groups for each, Group IA, IB, IIA, and IIB, eight rats for each. Group IA and IIA were not injected with dexamethasone, whereas Group IB and IIB were injected with dexamethasone 0.5 mg/kg BW/ day intramuscularly. At the end of the first week, blood was collected from eight rats (two rats for each) of Group IA, IIA, IB, and IIB for total leucocytes and blood Calcium analysis. Urine was also collected for urine Calcium analysis. Rats were then killed to collect the femur for histopathologic analysis using HE staining and immunohistochemistry to analyze the expression of TGF?-1 using Streptavidin-biotin method. Subsequent sampling was conducted at the end of 2nd, 4th, and 8th weeks of treatment, respectively. The total data of leucocytes, blood, and urine Calcium were statistically analyzed using a 2x2x4 factorial method, while the histopathologic and immunohistochemistry specimens were analyzed descriptively under the microscope based on the alteration of the bones. The statistical analysis showed that dexamethasone increased the concentration of urine Calcium (p<0,05), the concentration of blood Calcium insignificantly (p>0,05) affected by dexamethasone but cause homeostatic calcium disorder and the total leucocytes significantly (p<0,05) affected by grafted materials. Histopathologic analysis of the femur showed that there was an increased infiltration of inflammatory cells in the fracture area from the group untreated with dexamethasone. Whereas the immunohistochemistry analysis showed positive results from all groups. Based on the whole analysis, it can be concluded that although dexamethasone depressed inflammation process, it can also disturb the fracture healing process.
Avian Influenza Virus H5N1 Remained Exist in Gastrointestinal Tracts of House Flies 24 Hours Post-infection) (VIRUS FLU BURUNG H5N1 TETAP BERADA DALAM SALURAN PENCERNAAN LALAT RUMAH 24 JAM PASCAINFEKSI) Tisna Tyasasmaya; Hastari Wuryastuty; Wasito Wasito; Kai Sievert
Jurnal Veteriner Vol 17 No 2 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (93.156 KB)

Abstract

House flies (Musca domestica L.) are one of the major pests found in poultry farms resulting in not onlyannoyance and indirect damage to the poultry production but also transmitting many infectious organismsmechanically and biologically. A highly pathogenic avian influenza virus H5N1 (HPAIV H5N1) collectedfrom field house flies in Java Island, have fully succeeded identified and isolated in 2008. The AIV H5N1isolates were further used in the present study to determine the presence and persistence of the AIV H5N1in the laboratory infected house flies. One hundred house flies from a free AIV poultry farm in Yogyakarta,Indonesia were used in this study. The collected house flies were fasted for 12 hours then divided equallyin control and treated groups. The treated group was allowed to imbibe Dulbecco’s modified eagle medium(DMEM) containing AIV H5N1 while the control group imbibed medium without virus for one hour. Theflies from each group were collected at 12 and 24 hours post-exposure, respectively. All flies were immobilizedat 4°C, immersed in absolute ethanol for a few seconds and dissected under the stereomicroscope to collectthe gastrointestinal (GI) tracts. Based on the RT-PCR results, it is concluded that AIV H5N1 remainedexist in GI tracts of house flies for at least 24 hours post-exposure.
Chitosan Mempertipis Dinding dan Memperbesar Diameter Lumen Arteri Koroner Tikus Putih yang Diberi Pakan Lemak Tinggi (CHITOSAN COULD THINEN WALL AND WIDEN LUMEN DIAMETER CORONARY ARTERY OF SPRAGUE DAWLEY RATS INDUCED BY HIGH FAT RATION) Sri Isdadiyanto; Sukarti Moeljopawiro; Nyoman Puniawati; Hastari Wuryastuty
Jurnal Veteriner Vol 14 No 3 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (232.052 KB)

Abstract

The objective of this study was to analyze the influence of chitosan on coronary artery wall thicknessand lumen diameter of Sprague Dawley rats induced by high fat ration. The animals for this study weretwenty adult male rats divided into four groups, i.e. group I as the control was fed with basal rationcontaining normal fat for 3 months, group II was fed ration containing high fat for three months, group IIIwas fed ration containing high fat and given chitosan 180 mg per kg body weight per day orally in 2 mLaquadest for 3 months, group IV was fed ration containing high fat for three months and after one monthgiven chitosan 180 mg per kg body weight per day orally in 2 mL aquadest for two months. Each groupconsisted of five animals. After 90 days, the rats were necropsied and the hearts were collected tohistopathological. The difference between treatments was statistical analysis by Anava, and continued byLowest Significant Difference Test to locate the difference. Normal fat ration did not influence coronaryartery wall thickness and coronary artery lumen diameter in rats. High fat ration increased coronaryartery wall thickness and chitosan could be decreased coronary artery wall thickness in rats. High fatration + chitosan that given simultaneously could be increased coronary artery lumen diameter in rats.Based on the result of this study, it was concluded that high fat ration was a major factor able to causeatherosclerosis and chitosan was able to prevent atheroma plaque formation.
THE INFLUENCES OF CHITOSAN FROM Penaeus monodon ON C-REACTIVE PROTEIN EXPRESSION IN AORTA AND CORONARY ARTERY OF SPRAGUE DAWLEY RATS BY HIGH FAT INDUCTION Sri Isdadiyanto; Sukarti Moeljopawiro; Nyoman Puniawati; Hastari Wuryastuty
JOURNAL OF COASTAL DEVELOPMENT Vol 16, No 1 (2012): Volume 16, Number 1, Year 2012
Publisher : JOURNAL OF COASTAL DEVELOPMENT

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (610.937 KB)

Abstract

The objective of this study was to analyze the influences of chitosan on C-Reactive Protein expression in aorta and coronary artery of Sprague Dawley rats by high fat induction. The animals for this study were 20 adult male rats divided into four groups, i.e. group I as the control was fed with basal diet containing normal fat for 3 months, group II was fed diet containing high fat for 3 months, group III was fed diet containing high fat and given chitosan 180 mg per kg body weight per day orally in  2 ml aquadest for 3 months, group IV was fed diet containing high fat for 3 months and  after 1 month given chitosan 180 mg per kg body weight per day orally in 2 ml aquadest for 2 months. Each group consisted of five animals. After 90 days, the rats were necropsied and the hearts were collected to histopathological and immunohistochemical analysis by immunohistochemistry streptavidin-biotin method. C-Reactive Protein expression in aorta was negative. Chitosan was able to prevent atheroma plaque formation in coronary artery and CRP may involve in atherosclerosis.
Penurunan Glukosa dan Perubahan Profil Lipida Serum Tikus Sprague- Dawley Hiperglikemia-Hiperkolesterolemia Akibat Asupan Sorbitol-Oleat Poliester (SOPE) Agnes Murdiati; Hastari Wuryastuty; Y. Marsono; Eni Harmayani
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 3 (2009): October 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v14i3.2576

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Disorder of carbohydrate metabolism in the Diabetes Mellitus patients resulted in increasing of cholesterol synthesis. This condition increases the risk of heart attack or coronary heart disease, therefore it is recommended to lower fat intake. One of the alternatif to lower fat intake is by replacing fat with fat replacer in the diet. The aim of the present study is to evaluate the effect of replacing 75% of fat with SOPE on the glucose and lipid profile in diabetic-hipercholesterolemic rats. Eihgteen Sprague Dawley rats were devided into 2 groups of 9 rats. They were fed with hipercholesterol diet and were diabetic induced with alloxan IM injection. One group was fed with 75% SOPE substitute the fat in the standard diet and the other group was fed with standard diet. The result indicates that the oil substitution of standard diet of Sprague Dawley rats for sorbitol-oleic polyester as much as 75% gives an advantage to rats which suffer hiperglikemic-hypercholesterolemic because it decreased the glucose, total cholesterol, LDL cholesterol, and LDL/HDL cholesterol ratio, and did not decrease the HDL cholesterol, but increased triglyceride serum.
Pemeriksaan kesehatan kucing di Yogyakarta: Health Examination of Cat in Yogyakarta Soedarmanto Indarjulianto; Hastari Wuryastuty; Slamet Raharjo; Hary Purnamaningsih; Guntari Titik Mulyani; Ida Tjahajati
IGKOJEI: Jurnal Pengabdian Masyarakat Vol. 4 No. 1 (2023): IGKOJEI: Jurnal Pengabdian Masyarakat
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46549/igkojei.v4i1.337

Abstract

Cats are pets that many people, including in Yogyakarta, are keeping. Various kinds of diseases can attack cats, but not all owners are able to check their cat's health status. This community service aims to carry out health checks for cats owned by the community especially animal owners who are customers of the Veterinary Clinic of Internal Medicine, FVM-UGM. The service was carried out from April to October 2022 by checking the cat's health status directly. The service has been conducted and can carry out examinations and therapy of 20 cats. Based on the examination, it was found that 17 cats (85%) were diagnosed as sick and three healthy cats (15%). The three main diseases found were skin, digestive and urinary systems. Through medical examinations by students accompanied by veterinarians in the Department of Internal Medicine, cat owners have been invited to watch and given information regarding disease diagnosis, therapy, and how to administer medicine. The owner already understands the cat’s health status, agree to the action and treatment and can provide medicine for the sick cat. It was concluded that the community service for cat health examination had been successfully carried out on 20 cats, and the owner knew the condition of the cat and was able to give the medicine given. Key words: Cat Examination; Community Service; Veterinary Clinic
Co-Authors Agnes Murdiati Dhirgo Adji Eni Harmayani Guntari Titik Mulyani Hartiningsih . Hary Purnamaningsih Ida Tjahajati Irkham Widiyono Kai Sievert Laksono Trisnantoro Luh Made Sudimantini Nyoman Puniawati Prabowo Purwono Putro R. Wasito Soedarmanto Indarjulianto Soedarmanto Indarjulianto Sri Isdadiyanto Sri Isdadiyanto Sukarti Moeljopawiro Tisna Tyasasmaya Wasito Wasito Y. Marsono