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Variasi Morfologi dan Deteksi Leucocytozoon caulleryi dengan Metode PCR pada Ayam Ras di Wilayah Endemis Indonesia Endang Suprihati; Wiwik Misaco Yuniarti
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (8800.643 KB) | DOI: 10.22146/jsv.34666


The purpose of this study was to analyze the variation in morphology and detection Leucocytozoon caulleryi by PCR in mtDNA genes cty b that attacking bred chickens in endemic areas of Indonesia. This study was divided into three stages, data collection of Leucocytozoonosis cases in endemic areas of Indonesia; identification of parasites Leucocytozoon caulleryi morphologically through microscopic examination; and the identification of Leucocytozoon caulleryi cyt b genes that attack bred chickens in endemic areas by PCR. The results showed that there are variations in morphology of Leucocytozoon caulleryi that attacking chicken in endemic areas of Indonesia. Gamete morphometry of L. caulleryi had an average length and width 18.233 ± 4.672 dan 12.934 ± 3.349 µm. Nested PCR clearly showed positive reaction of Leucocytozoon infections by amplicons in 600 bp and 03 bp length. 
EFEKTIVITAS PEMBERIAN NANOPARTIKEL EKSTRAK DAUN JUWET (Syzygium cumini) SEBAGAI ADJUVANT TERAPI TERHADAP GAMBARANHISTOPATOLOGI PULMO MENCIT (Mus musculus) YANG DIINFEKSI Plasmodium bhergei Zerlinda Dyah Ayu; Lilik Maslachah; Koesnoto Supranianondo; Endang Suprihati; Hani Plumeriastuti; Rahmi Sugihartuti
Journal of Basic Medical Veterinary Vol. 9 No. 1 (2020): Journal of Basic Medical Veterinary, June 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v9i1.21091


The purpose of this study was to learn how to use juwet nanoparticle leaf extract (Syzygium cumini) as an adjunct therapy in pulmonary histopathological mice (Mus musculus) supported by Plasmodium berghei. This study used 30 rats divided into six groups consisting of K0 (disagree without agreement), P1 (fight without agreement), P2 (fight and dispute with chloroquine), P3 (fight and talk with juwet leaf extract), P4 (useful and useful with juwet leaf extract)) and P5 (extracts and combinations given with chloropine and juwet leaf extract nanoparticles) protected by 1x105 in 0.2 ml of Plasmodium berghei. Data were analyzed by Kruskal Wallis using SPSS and continued with the Mann-Whitney test. The results of this study showed that there were significant differences (p <0.05) between P2 and P5, but did not show a significant difference (p> 0.05) with P1, P3 and P4. Based on this study, can be witnessed by juwet nanoparticles (Syzygium Cumini) additional therapeutic leaf extract which is expected to reduce pulmonary damage (hemmoragi, alveolar congestion, edema, hemosiderin) mice (Mus musculus) infected with Plasmodium berghei.