Article Per Year (5 Year)
p-Index From 2019 - 2024
2.17
P-Index
This Author published in this journals
All Journal EKSAKTA: Journal of Sciences and Data Analysis Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Biomolecular and Health Science Journal Best Journal (Biology Education, Sains and Technology) Indonesia Berdaya Berkala Penelitian Hayati TAAWUN: Jurnal Pengabdian Kepada Masyarakat
Putri Ayu Ika Setiyowati
Biology Program, Faculty Of Science, Technology, And Education, Universitas Muhammadiyah Lamongan, Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

Published : 14 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 14 Documents
Search

 1   2 
Analysis Metallothionein of Carp fish in The Brantas River, Indonesia Putri Ayu Ika Setiyowati; Rofiatun Solekha; Sri Bintang Sahara; Febianti Dwi Hapsari
EKSAKTA: Journal of Sciences and Data Analysis VOLUME 1, ISSUE 2, August 2020
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/EKSAKTA.vol1.iss2.art8

Abstract

This study aimed to detection of protein profile, expression of metalothionein (MT) protein, and analyze difference of MT density in liver and gill in one of Carp fish that is Barbonymus balleroides in the upstream and downstream of Brantas river. The method used observasional analytic, Two individual of Barbonymus balleroides samples taken three times (with difference of month) from two station there are upstream and downstream of Brantas river. Analysis protein profile used electrophoresis SDS-PAGE (15%), expression and density of MT used western blot method and imageJ software. Difference between MT density in liver and gill of Barbonymus balleroides in the upstream and downstream of Brantas river analyzed with Two-way ANOVA. The results showed, protein profile in liver and gill Barbonymus balleroides in the upstream and downstream Brantas river have molecular weight about 8-93 kDa, expression of MT showed band of MT with molecular weight 24 kDa, results of MT density in liver and gill on upstream and downstream, continuously 231.29 MT/µm2 and 229.87MT/µm2, 232.41 MT/µm2 and  231.56 MT/µm2 but there is not significant.
Immunomodulator Effect of Lemongrass Extract (Cymbopogon nardus L.) to Increase Immune Cells as a Precaution Against SARS-CoV-2 Putri Ayu Ika Setiyowati; Rofiatun Solekha; Sri Bintang Sahara Mahaputra Kusuma Negara; Reny Rosalina
Biomolecular and Health Science Journal Vol. 4 No. 2 (2021): Biomolecular and Health Science Journal
Publisher : Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/bhsj.v4i2.26619

Abstract

Introduction: In humans, Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV-2) can damage some tissue when the immune systems was decrease. Natural product from the plant often used to improve immune response against microorganism including virus. This study aimed to determine the potential antioxidant of lemongrass extract (C . nardus) with various dosage that can provide immunomodulatory effects and find an optimal dosage to be used.  Methods: The method used observasional analytic, using animal model of 30 male mice strain BALB/C, weight 25-30 gram, divided into 5 groups; the positive control group was given 0.05 mL of  0.05% CMC within 14 days, negative control group was given IMBOOST® tablet 200 mg/kg body weight (bw) within 14 days, treatment groups  were given C. nardus extract with various doses 50 mg/kg bw, 150 mg/kg bw, and 300 mg/kg bw.  In day 21 all group were injected with 0,2 ml of  pathogen bacterial (S. aureus). Blood samples were taken three times: 7th day, 14th day, and 21th day. Results: The results showed that lemongrass extract (C. nardus) was able to influence the leukocyte and lymphocytes count with significant different (p<0.05). The optimal dose is 150 mg/kg body weight. Conclusion: The antioxidant compounds that contain in the C. nardus extract have an ability to increasing the immune system in the dose 150 mg/kg bw , but in the dose 300 mg/kg bw became toxic that can make a skin injury or death in animal test.
Protective Effect of Lemongrass Extract (Cymbopogon nardus) on Spermatozoa Cells Induced by Lead Acetate Putri Ayu Ika Setiyowati; Rofiatun Solekha; Dwi Dianita Irawan; Reny Rosalina; Karin Alifia Rachmadani; Angella Ananda Saputra
Biomolecular and Health Science Journal Vol. 5 No. 1 (2022): Biomolecular and Health Science Journal
Publisher : Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/bhsj.v5i1.31450

Abstract

Introduction: Male infertility can be caused by many factors. One of which is environmental pollution such as lead acetate. Lead acetate exposure can increase Reactive Oxygen Species (ROS), affecting spermatozoa quality. Natural antioxidants and flavonoid on Lemongrass (C. nardus) have a great potential for protecting the male reproductive system. This study aimed to analyze the effect of giving various dosages of C. nardus extract on the sperm quality of mice. Methods: The Balb/C male mice were divided into five equal groups. The negative control group was injected with 0.01 ml Na-CMC 0.5% within 40 days, and the positive control group was injected with 0.01 ml lead acetate 7 mg/kg BW within 5 days, the treatment groups were injected by 0.01 ml lead acetate 7 mg/kg body weight within 5 days and continuously injected by 0.01 ml of C. nardus extract with various dosage 25 mg/kg BW, 50 mg/kg BW, and 100 mg/kg BW within 35 days. At the end of the experiment, mice were sacrificed, and sperm suspensions were collected from cauda epididymal to measure the morphology, concentration, and motility.Results: The results showed that giving C. nardus extract could repair morphology, concentration, and motility of spermatozoa with significantly different (p<0.05). The 25 mg/kg BW dose has a good protective effect. Conclusion: The C. nardus extract can repair the decreasing sperm quality caused by lead acetate exposure.
Metallothionein analysis and cell damage levels on the liver and gill Of Barbonymus gonionotus In Brantas River, Indonesia Alfiah Hayati; Nita Yuliarini; Agoes Soegianto; Hana Widyana; Inesavira Rindaputri; Nuris Auliya; Putri Ayu Ika
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 23 No 1 (2017): December 2017
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (446.438 KB) | DOI: 10.23869/63

Abstract

This study aimed to analyze the levels of metallothionein and damage to hepatocytes and gills of Barbonymus gonionotus in the Brantas River (upstream and downstream), and the correlation levels of metallothionein in the gills and liver of Barbonymus gonionotus with different sampling time variation (March, June, and September 2016). Fishes were caught using trawl in two stations (Karangkates Reservoir and the Kali Jagir river). Fish gills and livers were taken for histopathological analysis and levels of metallothionein were measured by ELISA method. Gills histopathological analysis showed that the highest damage in Karangkates reservoir dan Kali Jagir river is hyperplasia. Liver histopathological analysis in Karangkates reservoir showed normal hepatocytes meanwhile necrosis is highest damage found in Kali Jagir river. Metallothionein levels of fish in Karangkates reservoir for March, June, and September were 0.18 ng/mL; 0,18 ng/mL; and 0.2 ng/mL (gills fish); 0,18 ng/mL; 0,34 ng/mL; and 0,21 ng/mL (liver fish) respectively. Metallothionein levels of fish in Kali Jagir river for March, June, and September were 0,26 ng/mL; 0,18 ng/mL; and 0,18 ng/mL (gills fish); 0,2 ng/mL; 0,45 ng/mL; 0,19 ng/mL (liver fish) respectively. We conclude that the damage to the gills and liver of Barbonymus gonionotus was mostly found in Kali Jagir River and the highest metallothionein level of Barbonymus gonionotus also presented in Kali Jagir River.
Efek Proteksi Ekstrak Cymbopogon nardus pada Histologi Testis Mencit (Mus musculus) Setelah Induksi Timbal Asetat Lilis Maghfuroh; Putri Ayu Ika Setyowati; Rofiatun Solekha; Khoirin Nisa; Nynda Ayu Nadira Savitri
Buletin Anatomi dan Fisiologi Volume 7, Nomor 1, Tahun 2022
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.7.1.2022.20-26

Abstract

Penelitian ini bertujuan untuk mengetahui efek perlindungan ekstrak serai wangi Cymbopogon nardus (C. nardus) terhadap induksi timbal asetat. Sebanyak 25 mencit jantan strain BALB/C secara acak dibagi menjadi lima kelompok. Kelompok kontrol negatif hanya mendapat injeksi sub cutan Na-CMC 0,5% selama 30 hari ; kontrol positif  diinjeksi sub cutan timbal asetat sebanyak 7 mg/kg bb selama 5 hari; P1, P2, dan P3 secara berturut-turut diinjeksi sub cutan timbal asetat 7 mg/kg bb selama 5 hari kemudian dilanjutkan dengan injeksi ekstrak C. nardus sebanyak 25, 50, dan 100 mg/kg bb selama 25 hari. Pada akhir penelitian semua mencit dikorbankan dan dilakukan koleksi testis. Jaringan testis diproses menggunakan pewarnaan Hematoxylin-Eosin. Hasil menunjukkan bahwa terjadi peningkatan sel spermatogonia, sel spermatosit, dan sel spermatid serta diameter tubulus seminiferus dibandingkan dengan kelompok positif. Dosis optimal yang paling mampu memperbaiki kerusakan jaringan testis yaitu 25 mg/kg bb. This study aimed to determine the protective effect of Cymbopogon nardus (C. nardus) extract against lead acetate exposure. A total of 25 male mice BALB/C were randomly divided into five groups. The negative control group only received 0.5% Na-CMC subcutaneously for 30 days; positive control was given 7 mg/kg bw of lead acetate subcutaneously for 5 days; P1, P2, and P3 were sequentially injected with 7 mg/kg bw subcutaneously for 5 days followed by injection of 25, 50, and 100 mg/kg bw of C. nardus extract for 25 days. At the end of the study all mice were sacrificed and testes were collected. Testicular tissue using Hematoxylin-Eosin staining. The results showed that there was an increase in spermatogonia cells, spermatocytes, and spermatid cells as well as the diameter of the seminiferous tubules compared to the positive group. The optimal dose most capable of repairing testicular tissue damage was 25 mg/kg bw.  
Uji Aktivitas Antibakteri pada Ekstrak Etanolik Teripang Kering dari Ujung Pangkah Gresik M. Ainul Mahbubillah; Putri Ayu Ika Setiyowati; Muhammad Badrut Tamam; Fika Nur Fitriana Putri Bukhori
BEST Journal (Biology Education, Sains and Technology) Vol 5, No 1 (2022): Juni 2022
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v5i1.5233

Abstract

Sea cucumber is a potential fishery sub-sector, but its use still not popular because of its unfavorable physical form. However, from its unfavorable form, sea cucumbers contain many benefits as nutrition and medicine, including the presence of antimicrobial activity. The use of sea cucumbers as a nutrient is used by the people of Ujung Pangkah Gresik village as crackers. This study aims to prove the antimicrobial potential of dried sea cucumbers with crackers from Ujung Pangkah village, Gresik. The sea cucumbers have been boiled first before drying in the sun. The dried sea cucumbers were blended and then extracted using 96% ethanol. The extract was then sterilized by filtration using a 0.2 m PTFE filter. Extracts were tested using the Kirby-Bauer method on Mueller-Hinton Agar medium inoculated with Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhimurium, Burkholderia cepacia, and yeast Candida albicans. The control antibiotic tetracycline 30 µg/ml and antifungal 2,5 µg/ml fluconazole were used as comparisons. The results of this study showed that all ethanolic extracts of dry sea cucumber with concentrations of 5%, 10%, and 15% did not show any clear zone. While the clear zone was shown in the control of tetracycline and fluconazole. This was caused by the loss of antimicrobial activity in the ethanolic extract of dried sea cucumbers derived from antimicrobial peptides which were damaged by heating during boiling process.
Phytochemical Screening of Ethanol Extract on Stems, Leaves and Roots of Citronella Grass (Cymbopogon nardus L) Rofiatun Solekha; Putri Ayu ika Setiyowati; Sri bintang Sahara Mahaputra Kusumanegara; Chandra Tri uliana Sari
BEST Journal (Biology Education, Sains and Technology) Vol 5, No 1 (2022): Juni 2022
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v5i1.5320

Abstract

Citronella grass is a plant that belongs to the rhizome group. In its application, lemongrass is widely used for both food, medicine and aromatherapy needs. The various benefits of lemongrass are interesting to research about the content of compounds in lemongrass. The purpose of this study is to provide information about screening for chemical compounds in the ethanol extract of citronella (Cimbopogon nardus L). This research method is by way of Phytochemical Screening test, namely Alkaloids with Mayer, Dragendorff, and Bouchardat reagents. Flavonoid test with Shinoda test, tannin test with gelatin test, saponin test with foam test, terpenoid/steroid test with Liebermann-Burchard reagent. The results showed that phytochemical screening showed the presence of several compounds in the serum, namely alkaloids, tannins, saponins, triterpenoids and flavonoids in every member of the plant body, namely stems, roots and leaves, but did not contain steroids in the roots, stems and leaves.
Efek Pericarpium Manggis (Garcinia mangostana L.) Terhadap Protein Spermatozoa Epididimal Mencit Setelah Dipapar 2-Methoxyethanol Putri Ayu Ika Setiyowati
BEST Journal (Biology Education, Sains and Technology) Vol 3, No 2 (2020): September 2020
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v3i2.2809

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak fraksi pericarpium manggis (Garcinia mangostana L.) dengan variasi kepolaran dan dosis terhadap profil protein epididimal spermatozoa mencit (Mus musculus) yang terpapar 2-Methoxyethanol. Penelitian ini menggunakan 50 ekor mencit jantan strain BALB/C dengan berat badan 25-30 gram yang dibagi menjadi 8 kelompok; kelompok kontrol positif diberi 0,05 ml larutan CMC 0,05 % selama 40 hari , kelompok kontrol negatif diberi 200 mg/kg BB 2-Methoxyethanol selama 35 hari, kelompok perlakuan (P1, P2, P3, P4, P5, dan P6) diberi 200 mg/kg BB 2-Methoxyethanol selama 5 hari, dilanjutkan dengan pemberian ekstrak fraksi dan dosis, kelompok P1 dan P2 diberi ekstrak fraksi non polar pericarpium manggis dengan dosis 0,6 mg/kg BB dan 3 mg/kg BB selama 35 hari , P3 dan P4 diberi ekstrak fraksi semi polar dengan dosis 4 mg/kg BB dan 20 mg/kg BB selama 35 hari, P5 dan P6  diberi ekstrak fraksi polar pericarpium manggis dengan dosis 0,4 mg/kg BB dan 2 mg/kg BB selama 35 hari. Protein diisolasi dari spermatozoa yang dikoleksi dari epididimis, kemudian diukur konsentrasinya dan dianalisis menggunakan elektroforesis SDS-PAGE. Data yang diperoleh dianalisis secara deskriptif dengan membandingkan profil protein kelompok kontrol dan kelompok perlakuan. Hasil penelitian menunjukkan bahwa ekstrak fraksi polar pericarpium manggis dengan dosis 0,4 mg/kg BB dapat berpengaruh dalam memulihkan profil protein (20 kDa) dari spermatozoa epididimal mencit setelah terpapar 2-Methoxyethanol
Uji Ketahanan dan Total Alkaloid Tembakau (Nicotiana tabaccum) Setelah Infeksi Ralstolnia solanacearum Rofiatun Solekha; Putri Ayu Ika Setiyowati; Dimas Arya Nugraha; Karin Alifia Rachmadani
BEST Journal (Biology Education, Sains and Technology) Vol 4, No 1 (2021): Juni 2021
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v4i1.3588

Abstract

Ralstolnia solanacearum is solanacearum is non forming, gram negative, aerobic plant pathogen that causes wilting in various host plant. Ralstolnia solanacearum is non these bacteria cause wilt disease in tabacco plants which can cause death by up to 50%. In tobacco plants, resistance analysis in the form of alkaloid content is required, the use of alkaloids in plants is as protection form pest attacks, plant reinforcements and hormone regulators. This study aims to analyze the resistance after developing tobacco wilt disease after Ralstolnia solanacearum infection. This study used kemloko 2 and kemloko 3 varieties as resistant treathments, kemloko 1 varieties which were susceptible to being used as negative controls, and moderately resistant Sindoro 1 varieties as positive controls. Reliability analysis using IP and AUDPC than the alkaloid cntent analysis using chloroform. The result showed the kemloko 3 variety tobacco had 103, 40 value; kemloko 2 11,74; sindoro 1 205,76; and kemloko 1 has value of AUDPC 350,22. Kemloko 3 is the most resistant variety after Ralstolnia solanacearum infection.  The result of the analysis showed that the highest total alkaloid levels were found in kemloko 3, namely 15,760 ± 0,51 mg equivalent to the value of caffeine / gram. This shows that there is a correlation between the resistance of a plant to the many alkaloid compounds in the plant. The more resistant, the more lacloid compound content.  
Pengolahan Limbah Kotoran Kambing dan Penambahan Agen Hayati Menjadi Pupuk Kompos di Desa Solokuro, Kabupaten Lamongan Putri Ayu Ika Setiyowati; Aisyah Hadi Ramadani
Indonesia Berdaya Vol 4, No 2 (2023)
Publisher : Utan Kayu Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47679/ib.2023464

Abstract

Agriculture is an unavoidable human activity, as all people require food to survive. As time passes, more and more chemicals for agricultural production are becoming available to farmers, which will gradually diminish crop yields. Therefore, it is vital to provide counseling and training on producing organic fertilizer using the plentiful goat manure waste in Solokuro Village. This course aims to expand knowledge of how to manufacture compost from goat dung waste as an organic fertilizer on food crops on farms and in yards. Counseling and training techniques were utilized in the implementation of the program. Providing detailed information on the benefits of goat dung waste is one counseling method. As part of the training, the community was provided with information on how to manufacture compost and include biological agents through fermentation procedures packaged as practice and simulation. The results of the compost fertilizer advice and training were quite successful. Participants were very enthusiastic and eager to learn about goat dung waste processing processes that may be utilized to create compost, as they were previously unaware of such methods. In addition, farmers understand the characteristics of soil and the many types of organic fertilizers, as well as the methods for producing and applying them on fields. Abstrak: Pertanian merupakan kegiatan yang tidak bisa dihindari manusia, karena semua manusia perlu makan sebagai keberlanjutan hidupnya. Seiring berjalannya waktu, berbagai bahan kimia untuk produksi pertanian semakin bermunculan dikalangan para petani yang lambat laun akan menurunkan produktifitas hasil panen. Sehingga perlu dilakukan penyuluhan dan pelatihan pembuatan pupuk organik yaitu dengan memanfaatkan limbah kotoran kambing yang keberdaannya melimpah di Desa Solokuro. Pelatihan ini bertujuan untuk meningkatkan pemahaman tentang cara pembuatan pupuk kompos dari limbah kotoran kambing sebagai pupuk organik yang dapat digunakan pada tanaman pangan di lahan pertanian maupun pekarangan. Pelaksanaan program dilaksanakan dengan menggunakan metode penyuluhan dan pelatihan. Penyuluhan dilakukan dengan memberi pengetahuan khusus tentang manfaat limbah kotoran kambing. Sedangkan pelatihan dilakukan dengan memberikan informasi kepada masyarakat tentang cara pembuatan kompos dengan penambahan agensia hayati melalui teknik fermentasi yang dikemas dalam bentuk praktek dan simulasi. Hasil penyuluhan dan pelatihan pembuatan pupuk kompos berjalan dengan sangat baik. Peserta sangat antusias dan tertarik untuk mengetahui semuanya karena selama ini belum tahu tentang teknik pengolahan limbah kotoran kambing yang efektif dan dapat dimanfaatkan untuk pembuatan pupuk kompos. Tidak hanya itu, petani juga memahami karakteristik tanah maupun jenis-jenis pupuk organik sekaligus teknik pembuatan dan pengaplikasiaannya di lahan pertanian. 
Co-Authors Agoes Soegianto Aisyah Hadi Ramadani Aisyah Hadi Ramadani Alfiah Hayati Angella Ananda Saputra Arsanul Khakim Ayu Dewi Wulandari Badriyatul Musyarofah Bagus Dwi Jauhari Chandra Tri uliana Sari Chasanah Uswatun Dimas Arya Nugraha Dwi Dianita Irawan Dwi Zulianti Dyah Ayu Sri Hartanti Febianti Dwi Hapsari Fika Nur Fitriana Putri Bukhori Filliana Andalucya Filliana Andalucya Hana Widyana Imam Hanafi Inayah Fitri Inesavira Rindaputri Karin Alifia Rachmadani Karin Alifia Rachmadani Khoirin Nisa Lilis Maghfuroh M. Syahrur Ramadhan Mahbubillah, M. Ainul Mufti Ari Bianto Muhammad Badrut Tamam Nita Yuliarini Nur Fatimah Azzahra Azzahra Nuris Auliya Nynda Ayu Nadira Savitri Putri Gita Ayu Savitri Reny Rosalina Rieke Dwi Ayuni Rofiatun Solekha Salasun Nisa Salasun Nisah Sri Bintang Sahara Sri Bintang Sahara Mahaputra Kusuma Negara Sri bintang Sahara Mahaputra Kusumanegara Syafiq Alfanani Trisnani Alif