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Kemampuan Bakteri Pelarut Fosfat Isolat Asal Sei Garo dalam Penyediaan Fosfat Terlarut dan Serapannya pada Tanaman Kedelai Wahyu Lestari; Tetty M. Linda; Atria Martina
Biospecies Vol. 4 No. 2 (2011)
Publisher : Universitas Jambi

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Bakteri pelarut fosfat dari tanah gambut asal Sei. Garo telah diuji potensinya padamedium Pikovskaya dan tanah PMK. Delapan isolat berhasil didapatkan, 3 diantaranya memilikikriteria tinggi melarutkan fosfat. Isolat dengan kriteria tinggi dan campurannya diuji potensinya dalampenyediaan fosfat pada medium Pikovskaya cair dan tanah PMK. Kemampuan tertinggi melarutkanfosfat pada medium Pikovskaya cair adalah isolat GGO1 (I1) yaitu 0,4736%. Secara keseluruhan adakecenderungan bahwa inokulum memiliki kemampuan dalam penyediaan fosfat pada tanah PMKnamun hasil menunjukkan tidak ada interaksi antara tanah dan inokulum terhadap kandungan fosfattersedia tanah dan serapannya pada tanaman. Perlakuan tanah tanpa sterilisasi berpengaruh nyataterhadap kandungan fosfat tersedia tanah.

UJI EFEKTIVITAS JAMUR Metarhizium anisopliae Cps.T.B ISOLAT LOKAL TERHADAP RAYAP (Coptotermes curvignathus) Rozalia '; Atria Martina; Titrawani '
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Termite is one of the major pest on oil palm plantations, especially in peat soil. Application of synthetic insecticides will cause resistant insect, enviromental pollution, and other problems. The entomopathogenic fungus Metarhizium anisopliae has been reported to infect many insects, including termite. This study was aimed to analyze the effectiveness of treatment of M. anisopliae Cps.T.B and M. anisopliae Cps.T.B with zeolit in controlling termite, C. curvignathus. This research used a completely randomized design with three treatments and eight replications. The result showed that spore density of M. anisopliae Cps.T.B with zeolite was 2,5 x108 spores/g and M. anisopliae Cps.T.B without zeolite was 3,2 x108 spores/gram respectively. Germination ability of M. anisopliae Cps.T.B with zeolite was 91.11% and M. anisopliae Cps.T.B without zeolite was 87.41% respectively. Mortality of C. curvignathus on eight days after treatment using M. anisopliae Cps.T.B was 100% and using M. anisopliae Cps.T.B with zeolite was 95,5% while the mortality of C. curvignathus a control was 15%.

SELEKSI DAN UJI ANTIBAKTERI AKTINOMISETES ASAL TANAH GAMBUT RIMBO PANJANG KAMPAR RIAU TERHADAP Escherichia coli DAN Salmonella typhi Siti Suryani; Rodesia Mustika Roza; Atria Martina
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Actinomycetes is one of microorganisms that can be used as a natural antibiotic. Metabolites produced by actinomycetes have antagonistic activity on bacteria, therefore it can be used as antibacteria. The purposes of this study were to select and test the antibacterial activity of actinomycetes isolated from peat soil in Desa Rimbo Panjang Kabupaten Kampar Riau to inhibit the growth of Escherichia coliand Salmonella typhi. This study was conducted from December 2013-January2014 at the Microbiology Laboratory, University of Riau. Serial dilutions and pour plate method used for isolation of actinomycetes while agar disk method used for antibacterial test. The result found a total of 24 actinomycetes isolates, 10 isolates inhibiting E. coli, and 16 isolates inhibiting S. typhi. The inhibition zone diameter of E. coliand S. typhi were 8,3 to 16,9 mm and 7,8 to 12,9 mm, respectively.

UJI AKTIVITAS ANTIFUNGAL KULIT MANGGIS (Garcinia mangostana) DAN SEMANGKA (Citrullus vulgaris)TERHADAP Trichophyton mentagrophytes PENYEBAB DERMATOMYCOSIS Tengku Putri Sholihah; Atria Martina; Yuharmen '
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 2, No 1 (2015): Wisuda Februari 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Dermatomycosis is the most common infection diseasesuffered by inhabitants of tropical countries and caused by Trichophyton mentagrophytes. Mangosteen and watermelon epicarp have high phytochemical compounds which is potential as antibacterial, antifungal and antioxidant. This study aimed to determine antifungal activity of mangosteen and watermelon epicarp against Trichophyton mentagrophytes. The study was conducted using completely randomizedfactorial design. Epicarp of mangosteen and watermelon were processed to fresh and dry extracts with concentration 40% and 80% (fresh extract) and 5%, 10% and 20% (dry extract). Dry extract were dissolved using 3 different solvent, e.g: aquabides, heated aquabides and etyl alcohol. Antifungal activity was tested using paper disc method. The results showed that almost all of watermelon and mangosteen epicarp dry extract had antifungal activity againts Trichophyton mentagrophytes, while fresh extract did not showed antifungal activity againts Trichophyton mentagrophytes. The highest antifungal activity of watermelon was showed in 20% extract in heated aquabides with inhibition zone diameter is 36,8 mm. The highest antifungal activity of mangosteen was showed in 10% extract in heated aquabides with inhibition zone diameter is 28,5 mm.

ISOLASI DAN SELEKSI BAKTERI ASAM LAKTAT DARI YOGHURT KEMASAN YANG BERSIFAT ANTIBAKTERI TERHADAP Escherichia coli DAN Salmonella typhi Ike Yuliana; Rodesia M. Roza; Atria Martina
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 1 (2014): Wisuda Februari 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Yoghurt is a fermented product of a group of lactic acid bacteria (LAB) on milk that hasbeen pasteurized. LAB is a group of bacteria that are able to convert carbohydrates(glucose) into lactic acid. LAB produces metabolites that inhibit the growth ofpathogenic bacteria. This study aimed to isolate LAB from yoghurt packaging with itsinhibitory against bacterial pathogens Escherichia coli and Salmonella typhi. LAB wasisolated from 9 samples with 3 brand products and 3 different flavors. Calculation of thetotal population to the overall LAB samples was obtained between 6.4 × 10 6 cfu/ml to2.1 × 10 7 cfu/ml. In this study, inhibition test of pathogenic bacteria E. coli and S. typhiused samples yoghurt. The method used is the well-agar diffusion. The most effectiveyoghurt in inhibiting E. coli was yogurt Ab with diameter of inhibiting zone 23.55 mm,while the yogurt sample that had the highest inhibitory zone which is 19.93 mm forS.typhi was yoghurt Ap.

ISOLASI DAN KARAKTERISASI FUNGI ENDOFIT DARI KULIT BUAH MANGGIS (Garcinia mangostana L) SEBAGAI ANTIMIKROBA TERHADAP Candida albicans, Staphylococcus aureus DAN Escherichia coli Dewi Elfina; Atria Martina; Rodesia Mustika Roza
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 1 (2014): Wisuda Februari 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Garcinia mangostana Linn is one of the popular local plants that has been used asmedicinal herb. Extract of Garcinia mangostana has been reported having anti-inflammatory, anti-tumour, anti-oxidant and antimicrobial activities. Bioactivecompounds from extract of mangosteen fruit husk were thought to produce byendophytic fungi. The purpose of this research were to isolate and characterizeendophytic fungi from mangosteen fruit husk that potential to produce antimicrobialcompound. Antimicrobial activity was determined by measuring the inhibition zonewith Agar Disc and Kirby-Bauer assay methods using pathogenic microbes i.e. Candidaalbicans, Staphylococcus aureus and Escherichia coli. Eleven of twenty isolatesendophytic fungi which were obtained from mangosteen fruit husk were known to haveantimicrobial activities. Endophytic fungi producing antimicrobial belongs to genusAspergillus, Penicillium, Trichoderma, Alternaria and Fusarium. Endophytic fungihaving the highest antimicrobial activities in agar disc assay were Penicilliumsp.1KMA, Aspergillus sp.5KMR, Aspergillus sp.1KMA, Fusarium sp.KMR andAspergillus sp.3KMB. Endophytic fungi having the highest antimicrobial activities inKirby-Bauer assay was Penicillium sp.1KMA. Antimicrobial activities of Penicilliumsp.1KMA was significantly different with the other four endophytic fungi.

PENGENDALIAN Ganoderma boninense OLEH Trichoderma sp. SBJ8 PADA KECAMBAH DAN BIBIT KELAPA SAWIT (Elaeis guineensis Jacq.) DI TANAH GAMBUT Rizky Alviodinasyari; Atria Martina; Wahyu Lestari
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 2, No 1 (2015): Wisuda Februari 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Basal stem rot disease caused by Ganoderma boninense is the most destructive disease in oil palm plantation in Indonesia, especially in the peatland. The use of chemical pesticides as controlling agent is hazardous to environment and human health. The alternative to control this disease is by using biological agent Trichoderma sp. SBJ8 in the form of Ganofend biofungicide. The research were aimed to study the effectivity ofTrichoderma sp. SBJ8 isolate in suppressing in vitro growth of G. boninense, as well as to determine the ability of Ganofend biofungicide in inhibiting the growth of G. boninense on the oil palm germination and seedling. The experimental study included two cultures i.e. in vitro and in vivo using randomized design with 6 treatments: without Ganofend biofungicide treatment, 100 g Ganofend biofungicide treatment, and with immersing in Ganofend biofungicide solution for both root and seedling of oil palm. The results showed that the isolates of Trichoderma sp. SBJ8 could inhibit the growth of G. boninense in 4th day up to 65,25%. The treatment using Ganofend biofungicide was effective to decrease the mortality percentage of oil palm germination and not causes seedling death.

ANALISIS FISIOLOGI JAMUR LIGNINOLITIK DAN SELULOLITIK ASAL TANAH GAMBUT DESA RIMBO PANJANG KABUPATEN KAMPAR SEBAGAI AGEN BIOKOMPOS Karina Larasati; Rodesia Mustika Roza; Atria Martina
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Cellulolytic and ligninolytic fungi have an important role in degradation of organic materials, especially lignin and cellulose, in a composting process. The success of the fungi in the composting process is strongly influenced by temperature and pH. The purpose of this research was to obtain isolates of the fungi with the best ability to be applied as biocompost agent by analyzing its ability to grow in different temperature and pH. A total of 110 isolates from peatsoil in Rimbo Panjang, Kampar Regency, Riau was inoculated into Potato Dextrose Broth medium with pH of 5 and pH of 7 then incubated for 3 days at room temperature (±27ºC), 40ºC, 50ºC, 60°C, 70ºC and 80ºC. The results obtained 110 isolates which were grown at room temperature (±27ºC) with pH of 5 and pH of 7 and 96 isolates which were grown at 40ºC temperature. A total of 27 isolates which were grown at 50ºC temperature consisted of genus Penicillium, Trichoderma, and Aspergillus.

ISOLASI DAN SELEKSI BAKTERI ASAM LAKTAT DARI YOGHURT PRODUKSI INDUSTRI RUMAH TANGGA DI PEKANBARU YANG BERSIFAT ANTIBAKTERI TERHADAP Escherichia coli DAN Salmonella typhi Lili Yani; Rodesia Mustika Roza; Atria Martina
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 1 (2014): Wisuda Februari 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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The occurance of Lactic Acid Bacteria (LAB) in yoghurt produced by home industry inPekanbaru as probiotic potentially improve intestinal physiological function, useful forhealth as well as prevention of the growth of pathogenic bacteria. The purposes of thisstudy were to calculate the total LAB population of yoghurt with the effect of mango andstrawberry juice during different storage process, and also to determine the ability ofantibacterial activity against Escherichia coli and Salmonella typhi. The population of LABin yoghurt was 4,0 × 10 7 – 2,9 × 10 8 CFU/ml. Antimicrobial activity assay used the welldiffusion method. The mango juice yoghurt incubated in 7 day was the most effective forEscherichia coli with the inhibition zone 27,1 mm. While the mango juice yoghurtincubated in 3 day was the most effective for Salmonella typhi with inhibition zone 29,9mm.

UJI POTENSI ANTIFUNGI AKTINOMISETES SELULOLITIK DAN LIGNINOLITIK DAN BAKTERI LIGNOSELULOLITIK ISOLAT LOKAL TERHADAP PERTUMBUHAN JAMUR Ganoderma boninense DAN Colletotrichum capsici Dede Martin; Atria Martina; Rodesia Mustika Roza
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 2, No 1 (2015): Wisuda Februari 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Colletotrichum capsici fungi, causing agent of anthracnose in chili, and Ganoderma boninense, fungi causing agent of basal stem rotten in oil palm, can reduce the productivity of chili and oil palm. These disease are increasing so that it is necessary to find local biological agents that are environmentally friendly. The purpose of this research was to determine the potential isolates of lignocellulolyltic bacteria and cellulolytic and ligninolytic actinomycetes from peat soil of Rimbo Panjang Kampar, Riau as an antifungal agent to inhibit the growth of C. capsici and G. boninense.Antifungal activity was screened using agar disc method by measured the inhibition zone for seven days. The results showed that 13 isolates of actinomycetes haveantifungal activity against C. capsici with the highest inhibition zone 13,3 mm by RB2S40. Six isolates of actinomycetes have antifungal activity against G. boninense with the highest inhibition zone 29,15 mm by RB1S4. Five isolates have the ability to inhibit both fungi which were targeted.

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