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All Journal Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan
HEDI INDRA JANUAR
Balai Besar Penelitian dan Pengembangan Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

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Correlation between fucoxanthin contents in Turbinaria sp.and sea water nutrients at Binuangeun and Krakal Coasts Januar, Hedi Indra; Wikanta, Thamrin
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 6, No 1 (2011): May 2011
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v6i1.57

Abstract

Fucoxanthin pigment is a potential compound in nutraceutical. It is commonly found in algaesuch as in Turbinaria sp.,a very abundant species in Indonesian coastal. To maximize theproduction of fucoxanthin for the development of nutraceutical industry in the coastal area, it isimportant to identify the optimal environmental parameters for its biosynthesis. It has been reportedthat nitrogen and or phosphate nutrients affect the growth of algae. Therefore, this paper aimed toobtain an optimal environment for Turbinaria sp. to produce fucoxanthin based on correlationanalysis. Correlated data were fucoxanthin isolated from Turbinaria sp.that was collected fromKrakal (Yogyakarta) and Binuangeun (Banten) coasts. Fucoxanthin contents in each sample werecompared based on the peak areas in chromatogram after 1 mg injection of n-hexane extract ofT. decurrrens in the HPLC system. The analysis results showed that the peak areas of fucoxanthinwere significantly different among sampling locations (P = 7.99 x 10-6), sampling periods (P = 6 x10-3) and interaction of both (P = 3 x 10-3). Whereas the correlation analysis revealed that interactionbetween phosphate and temperature could be a predictor that strongly (R = 0.986) and significantlycorrelated (P = 1.09 x 10-7) with the peak areas of fucoxanthin (R2= 96.5%). These results were inline with growth limiting factor in seawater and the function of fucoxanthin as ultraviolet protector.Based on these results, it is suggested that the suitable location for fucoxanthin production is in thearea of agricultural run-off that contains an elevated concentration of dissolved phosphate.
STUDY ON THE EFFECT OF POLLUTANTS ON THE PRODUCTION OF AAPTAMINES AND THE CYTOTOXICITY OF CRUDE EXTRACT FROM Aaptos suberitoides Dewi, Ariyanti Suhita; Hadi, Tri Aryono; Januar, Hedi Indra; Pratitis, Asri; Chasanah, Ekowati
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 7, No 3 (2012): December 2012
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v7i3.4

Abstract

This experiment was conducted to study the effects of anthropogenic stressor on the spatialvariability of secondary metabolites from marine sponge Aaptos suberitoides. Samplings wereconducted at 7 sites in Marine National Park of Thousand Islands that are extended within 30 kmoff Jakarta bay on late February 2011. Sponges were collected and quantified by means of liquidchromatography coupled with photo-diode array detection, whereas, cytotoxicity of sponges extractswas determined against T47D (breast) cancer cell lines. Results showed that the spatialproduction of aaptamine and isoaaptamine did not correlate with the quality of their surroundinghabitat, despite nitrite and nitrate levels significantly affected the bioactivity of crude extracts.
Composition of Fatty Acids in Evaluation of Sea Cucumber Potency for Nutraceutical Product Development Susilowati, Rini; Pratitis, Asri; Januar, Hedi Indra
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 11, No 2 (2016): August 2016
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v11i2.238

Abstract

Unsaturated fatty acid in food has an effect to improve human health, while saturated fatty acid has the opposite impact. Ratio between both types of fatty acids is important to evaluate the biological material for nutraceutical product development. This paper aimed to evaluate the nutraceutical potency of sea cucumber from Kendari Bay Waters, South East Sulawesi, based on their fatty acids composition. The analysis was conducted by the 1H-NMR (Hidrogen-Nuclear Magnetic Resonance) method. Several species within 4 genera (Holothuria, Bohadschia, Actinopyga, and Stichopus) were selected as the subject of the study. Comparison of fatty acid composition has been done in genera level, the preliminary study found that variation between species among the same genera was insignificant (p >0.05). Results of the study detected that Holothuria contained with an even ratio of Poly Unsaturated Fatty Acids (PUFA) and Saturated Fatty Acids (SFA), but Actinopyga was detected with low PUFA/SFA ratio. It may suggest that Holothuria from this region is the most potential raw material for nutraceutical product development. Meanwhile, precaution is needed for genera that contained with low PUFA/SFA ratio. Furthermore, comparison with other studies detected that fatty acids composition is not morphological specific, but vary, according to food viability, environmental and geographic location. Therefore, preliminary screening is important in evaluation of sea cucumber material for nutraceutical product development.  
Logic Structure Determination (LSD) as a Computer Assisted Structure Elucidation (CASE) for Molecular Structure Determination of Cytotoxic Cembranoids from Soft Coral Januar, Hedi Indra; Zamani, Neviaty Putri; Soedharma, Dedi; Chasanah, Ekowati
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 11, No 1 (2016): May 2016
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v11i1.177

Abstract

Indonesian tropical soft corals are valuable resources that produce pharmacological cytotoxic cembranoids. However,  the manual structure determination in these compounds requires adequate knowledge of organic chemistry. This study presents the application of Logic Structure Determination (LSD) as a freeware Computer Assisted Structure Elucidation (CASE) for automatic molecular structure determination of cembranoid compounds from soft corals species. 12 Nuclear Magnetic Resonance (NMR) datasets of cytotoxic cembranoids were used to evaluate the accuracy of LSD in generating the possible structures. The results of this study shows that LSD generated numerous possible molecular structures as the data input files were only derived from 2D-NMR HMQC (Heteronuclear Multiple-Quantum Correlation), COSY (Homonuclear Correlation Spectroscopy), and HMBC (Heteronuclear Multiple Bond Correlation). The accuracy was significantly enhanced (only 2-4 possible cembranoid structures from each NMR dataset) with the addition of H2BC (Heteronuclear 2 Bond Correlation) experimental data. This may indicate that VLRC (Very Long-Range Correlations) significantly affects LSD capability. Furthermore, LSD with direct 2 bond NMR experimental data is a reliable CASE technique for cembranoid compounds identification. In general, this freeware-CASE has the potential to be applied  on other types of small molecule compounds and may serves as a solution for elucidation bottleneck step in studies on Indonesian natural products.
Metabolite Profiles and Antioxidant Activity of Caulerpa racemosa with Different Handlings sihono, Sihono; Tarman, Kustiariyah; Madduppa, Hawis; Januar, Hedi Indra
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 3 (2018): December 2018
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v13i3.355

Abstract

 Metabolite profiles and antioxidant activity of Caulerpa racemosa extract with different handlings were investigated. Three different handlings during transportation were applied, namely samples chilled with ice, stored in liquid nitrogen and soaked in seawater. The different handling significantly affected the yield of ethanolic crude extracts and inorganic fractions but insignificantly to organic fractions. Different handlings resulted in differences of major fractions of C. racemosa extracts. Major fractions of the sample that was handled with chilling temperature contained low polar fractions (K10, K11, K12, and K13), while seawater handling extract contained very polar (K1, K2 and K3), polar (K6, K7, and K8) and low polar (K13) fractions. The extract of the sample handled in liquid nitrogen contained balanced fractions. Chilling temperature handling produced highest antioxidant activity (IC50 below 2,000 ppm) in ethanolic extract of C. racemosa. Keywords: antioxidant activity, Caulerpa racemosa, ethanolic extract,handlings, IC50
Bioprospeksi Spons, Karang Lunak dan Ascidian Asal Taman Nasional Laut Kepulauan Wakatobi: Antitumor dan Antioksidan Fajarningsih, Nurrahmi Dewi; Nursid, Muhammad; Januar, Hedi Indra; Wikanta, Thamrin
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 8, No 2 (2013): Desember 2013
Publisher : Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jpbkp.v8i2.60

Abstract

Penelitian ini bertujuan untuk mengetahui potensi manfaat (bioprospeksi) spons, karanglunak, dan ascidian asal Taman Nasional Laut (TNL) Kepulauan Wakatobi sebagai antitumor dan antioksidan. Sampel makroinvertebrata laut diambil dengan menggunakan scuba diving di empat stasiun pengambilan di perairan TNL Kep. Wakatobi. Sebanyak 74 sampel diuji aktivitas antitumornya terhadap 3 jenis sel lestari tumor (HeLa, MCF7, SKOV3) dengan metode Thiazolyl Blue Tetrazolium Blue (MTT) dan diuji pula aktivitas antioksidannya dengan menggunakan metode DPPH (2,2-diphenyl pikryl hidrazil). Sampel yang dapat menghambat >50% sel lestari tumor pada konsentrasi 20 µg/ml dikategorikan sebagai sampel aktif antitumor. Sampel yang memiliki aktivitas penghambatan radikal bebas >50% pada konsentrasi 100 µg/ml dalam uji DPPH dikategorikan sebagai sampel yang aktif antioksidan. Hasil penelitian menunjukkan bahwa terdapat 3 sampel (ascidian W0208; spons W2408 dan W2508) yang aktif terhadap tiga jenis sel lestari tumor, 11 sampel aktif terhadap dua jenis sel lestari tumor dan 22 sampel aktif terhadap satu jenis sel lestari tumor. Dari 74 sampel yang diuji aktivitas antioksidannya, terdapat 8 sampel yang tergolong aktif (7 sampel spons dan 1 sampel karang lunak).
Uji Aktivitas Antioksidan, Toksisitas, The Assays of The Antioxidative Activity, Toxicity, and Cytotoxicity of The Red Algae. Wikanta, Thamrin; Januar, Hedi Indra; Nursid, Muhammad
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 11, No 4 (2005): JPPI Ed Pascapanen
Publisher : Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jpbkp.v11i4.475

Abstract

Riset tentang aktivitas antioksidan, toksisitas dan sitotoksisitas ekstrak Rhodymenia palmata telah dilaksanakan. Uji aktivitas antioksidan dilakukan dengan metode DPPH (1,1‑ difenil‑2­pikrilhidrazil).Uji toksisitas terhadap Artemia salina dilakukan dengan metode BSLT (Brine Shrimp Lethality Test). Uji sitotoksisitas terhadap sel lestari tumor HeLa ditentukan dengan metode MTT (3‑(4,5‑dimetilthiazol‑2yi)‑2,5‑difeniI tetrazolium bromida). Hasil uji menunjukkan bahwa ekstralk kasar metanol memiliki aktivitas antioksidan rendah (IC .0~464,89 ppm). Fraksi etil asetat memiliki toksisitas paling tinggi (L C50 =5,36 ppm), dan fraksi n‑heksana memiliki toksisitas rendah (LC 0=55,20 ppm), sedangkan fraksi metanol termasuk kelompok tidak toksik (LC 50=39012,95 ppm). Uji sitotoksisitas terhadap sel lestari tumor HeLa menunjukkan bahwa fraksi etil asetat memiliki sitotolksisitas rendah (LC 5,=432,43 ppm), sedangkan fraksi n‑heksana memiliki sitotoksisitas tinggi (LC 5,=29,68 ppm).
VARIASI TEMPORAL KADAR SAKSITOKSIN DALAM KEKERANGAN DARI PERAIRAN TANJUNG BALAI, SUMATRA UTARA Januar, Hedi Indra; Dwiyitno, Dwiyitno; Annisah, Umi; Putri, Ajeng Kurniasari
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 14, No 2 (2019): Desember 2019
Publisher : Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (518.06 KB) | DOI: 10.15578/jpbkp.v14i2.613

Abstract

Kekerangan merupakan salah satu biota ekonomi penting di sektor perikanan. Namun, dengan sifatnya sebagai filter feeder, pencemaran lingkungan perairan dapat mempengaruhi keamanan pangannya, misalnya pencemaran senyawa saksitoksin, yang sering terakumulasi di biota kekerangan. Saksitoksin adalah senyawa yang dihasilkan oleh fitoplakton perairan, sehingga kadarnya dapat bervariasi secara temporal. Penelitian ini bertujuan untuk mengetahui variasi temporal  secara musiman (muson timur, peralihan, dan barat) dari kadar saksitoksin pada tiga jenis biota kerang ekonomis (Anadara granosa, Anadara antiquata, dan Polymesoda erosa) yang diperoleh dari Perairan Tanjung Balai. Kekerangan diketahui sebagai produk unggulan di sentra perikanan Tanjung Balai. Variasi temporal kadar saksitoksin dihubungkan dengan kualitas air, untuk mengetahui korelasinya terhadap lingkungan tempat hidup kekerangan. Kualitas air dianalisis secara in situ menggunakan metode potensiometri dan kolorimetri, sementara kuantifikasi saksitoksin dilakukan menggunakan teknik spektrometri massa. Hasil analisis memperlihatkan bahwa kadar saksitoksin dari ketiga jenis kerang bervariasi antara 0,04 hingga 0,16 mg STXeq/kg berat basah kerang. Hal ini menunjukkan kekerangan di wilayah ini aman dari bahaya saksitoksin (ambang maksimum 0,8 mg STXeq/kg). Kadar bahan berbahaya ini tidak secara signifikan (P>0,05) dipengaruhi oleh ukuran dan jenis kerang. Namun, akumulasinya di musim muson barat secara signifikan (P<0,05) lebih tinggi dibandingkan dengan musim muson timur dan peralihan. Hal ini diduga terkait dengan musim penghujan di muson barat yang meningkatkan polusi nutrien akibat limpasan terestrial. Kondisi ini diduga memicu peningkatan pertumbuhan fitoplakton, termasuk jenis penghasil saksitoksin, sehingga memicu peningkatan akumulasinya di kekerangan.
Co-Authors Annisah, Umi ARIYANTI SUHITA DEWI DEDI SOEDHARMA Dwiyitno Dwiyitno Ekowati Chasanah Hawis H Madduppa Kustiariyah Tarman Muhammad Nursid Neviaty P Zamani Nurrahmi Dewi Fajarningsih, Nurrahmi Dewi Pratitis, Asri Putri, Ajeng Kurniasari Rini Susilowati, Rini Sihono Sihono Thamrin Wikanta Tri Aryono Hadi