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JFMR (Journal of Fisheries and Marine Research) Vol 4, No 1 (2020): JFMR VOL 4 NO 1
Publisher : JFMR (Journal of Fisheries and Marine Research)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2020.004.01.5


Produksi ikan asap di Kabupaten Tuban tumbuh secara signifikan karena lokasi penjualannya dekat lokasi wisata.Salah satu kawasan penghasil ikan asap ini beradadi Desa Karangsari, Tuban. Permasalahan keamanan pangan masih belum diperhatikan oleh para produsen ikan asap yang terlihat dari tampilan produk dalam keranjang secara terbuka dan pengemasan yang seadanya.Hal ini memengaruhi daya simpan produk yang hanya bertahan 1-2 hari. Oleh karena itu, kegiatan Doktor Mengabdi Universitas Brawijayabertujuan untuk meningkatkan kualitas produk ikan asap (mikrobiologi dan organoleptik) melalui induksi teknologi pengemasan vakum pada kelompok usaha pengasapan ikan. Metode pelaksanaan program ini adalah penyediaan teknologi pengemasan vakum, penyuluhan dan pelatihan pada mitra, serta pendampingan dan pengawasan. Adanya peningkatan status keamanan pangan produk ikan asap melalui penerapan teknologi pengemasan vakum yang disimpan dalam tiga kondisi (suhu ruang, dingin dan beku) ini dievaluasi melalui uji kualitasbaik secara mikrobiologimaupun organoleptik. Hasil uji total bakteri dan organoleptik menunjukkan pengemasan vakum dapat mempertahankan kualitas produk menjadi lebih baik dan lebih diterima konsumen. Kombinasi antara pengemasan vakum dan penyimpanan pada suhu dingin danbeku dapat menghambat pertumbuhan bakteri secara optimal. Teknologi pengemasan vakum untuk produk ikan asap dapat meningkatkan keamanan pangan dannilai jual.
Characterization of Probiotics Isolated from Intestine of Mackerel Fish (Rastrelliger sp.) from Lembata Regency of East Nusa Tenggara Daten, Helena; Ardyati, Tri; Jatmiko, Yoga Dwi
The Journal of Experimental Life Science Vol 10, No 2 (2020)
Publisher : Graduate School, University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2020.010.02.04


The research aimed to isolate, characterize, and analyze the ability of lactic acid bacteria (LAB) potential as probiotics to produce hydrolase enzyme. The LAB was isolated using MRS agar by the spread plate method. The LAB characterization includes antimicrobial activity, tolerance to low pH, bile salt, salinity, autoaggregation properties, and ability to produce hydrolytic enzymes. The isolate which has the highest ability to inhibit Aeromonas hydrophila is KBP 3.3, while the isolate which inhibits the highest Streptococcus agalactiae is KBP 1.1.1. The KBP 3.3 and KBP 1.1.1 were able to survive at pH 1 for 24 hours with a survival rate of 93.6% and 98.3%. The KBP 3.3 and KBP 1.1.1 are tolerant to 7.5% bile salt concentrations for 24 hours of 99.46% and 99.11%. The KBP 3.3 is tolerant to 0.5 % salinity for 24 hours with the highest survival rate of 113.38%, while KBP 1.1.1 is 94%. The KBP 3.3 and 1.1.1 have autoaggregation properties of 92.18% and 87.84%. The KBP 3.3 produced the highest lipase enzyme, while KBP 1.1.1 produced the protease enzyme.Keywords: hydrolytic enzyme, lactic acid bacteria, mackerel, probiotic
Antimicrobial Activity of Combination Bacteriocin and Asam Sunti Extract (Averrhoa bilimbi L. fermented) Against Multidrug Resistant Escherichia coli in Lettuces (Lactuca sativa) Kimbal, Angie Via Resty; Jatmiko, Yoga Dwi; Ardyati, Tri
The Journal of Experimental Life Science Vol 11, No 2 (2021)
Publisher : Graduate School, University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.02.06


The ready-to-eat vegetables are often associated with the presence of multidrug-resistant (MDR) bacteria. This study aimed to evaluate the potency of bacteriocin, Asam Sunti extract, and their combination against MDR E. coli in lettuce. Their antimicrobial activity was assessed using the disk diffusion method and bacterial enumeration after direct application in pre-inoculated lettuce with MDR E. coli. The bacteriocin was produced by Lactobacillus plantarum BP102 at optimum production time or during the stationary phase at 18 h. These bacteriocins were able to inhibit five MDR E. coli isolates, while Asam Sunti extract and the combination of bacteriocin and Asam Sunti extract were only able to inhibit three MDR E. coli (LL1.2, LL3.11, and LL3.12) and (LL1.2, LL1.3, and LL3.11), respectively. In direct application to pre-inoculated fresh lettuce, higher inhibition of MDR E. coli was observed after applying the combination of bacteriocin and Asam Sunti extract with a ratio of 1:1 and 1:2, compared to bacteriocin alone. However, the inhibitory activity of this combination treatment was not significantly different (p>0.05) with the Asam Sunti extract alone. The highest rate of decrease in total bacteria in lettuces was 97% occurred in isolate LL1.2 with bacteriocin treatment alone, and isolate LL3.11 with combination treatment of bacteriocin and Asam Sunti extract (1:2). While on MCA media, the best reduction rate of 94% occurred in isolate LL1.2 with treatment using bacteriocin only, Asam Sunti extract only, and their combination (1:2). The inhibition of MDR E. coli in fresh lettuces by bacteriocin, Asam Sunti extract, and their combination was strain-dependent which was indicated by various inhibition results in all treatments.Key words: Asam Sunti extract, Bacteriocin, multidrug resistant.
Molecular Identification of Yeasts Isolated from Dadih by RFLP-PCR and Assessment on Their Ability in Utilizing Lactate YOGA DWI JATMIKO; MIGUEL DE BARROS LOPES; MARY D BARTON
Microbiology Indonesia Vol. 6 No. 1 (2012): March 2012
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5454/mi.6.1.5


A wide variety of yeasts have been involved in traditional fermented foods, and potentially contributed to the development of product properties. The existence of indigenous yeasts in dadih, a traditionally fermented form of buffalo milk of West Sumatera, has been reported but an accurate identification is still required to be conducted. This study was aimed to identify yeasts isolated from dadih using a molecular approach, and to evaluate their lactate utilization. A total of 51 isolates were characterized and identified as Pichia jadinii and Candida stellimalicola using PCR amplification of the 5.8S - internal transcribed spacer region combined with restriction fragment length polymorphism (RFLP) analyses and gene sequencing. The former species were the dominant one in this tested product. Their ability to utilize lactate was demonstrated, indicating that they could modify the sensory characteristics of dadih, and hence interact with the indigenous lactic acid bacteria in dadih. The restriction profiles of the dadih yeasts can be used as a data base for rapid identification of yeasts in the future. Further work is still needed to elucidate the dadih yeast ecology.
Prediction On Difference In Micro, Small Medium Enterprises-Revenue After Getting Vacum Frying And Spinner Assistance Asep Awaludin Prihanto; Rahmi Nurdiani; Yoga Dwi Jatmiko; Hidayatun Muyasyaroh
Journal of Innovation and Applied Technology Vol 6, No 2 (2020)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiat.2020.006.02.16


Doktor Mengabdi is a community service program at Universitas Brawijaya that is carried out to improve the welfare of the community. One of the efforts to help the community in completing its packaging is by providing assistance in the form of business equipment.  The purpose of this study is to investigate the difference in income of Small medium enterprises, Lele Sehat (IGF) which has been provided with the help of vacuum frying and spinners machines. Data retrieval was done through interviews and live observations. The data were tested for its normality and further analyzed using a paired t-test. The results showed that there was a difference in income of small-medium enterprises difference between before and after getting tool assistance. Prior income was IDR 3,007,000. It was increased to IDR. 4,097,000. This indicates that providing tools to small-medium enterprises will increase income and indirectly improve their welfare.
Isolation and Identification of Nitrogen-Fixing Rhizobacteria associated with Cocoa plantation (Theobroma cacao L) as Biofertilizer Agent Nurul Afiyatul Jannah; Irfan Mustafa; Yoga Dwi Jatmiko
The Journal of Experimental Life Science Vol. 12 No. 3 (2022)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2022.012.03.01


Ringinkembar Village, Sumbermanjing Wetan District, Malang Regency, is one of the centers for cocoa plantations using an organic farming system. However, over time this organic farming system experienced a decrease in fruit production, possibly from soil fertility and biofertilizers that were less available in the soil. This study aims to analyze the nitrogen-fixing ability and identify rhizosphere isolates that excel in nitrogen-fixing obtained from the rhizosphere of cacao (Theobroma cacao L) plant. Bacteria were isolated from the soil surrounding cocoa plant roots and grown on Nfb (Nitrogen free Bromothymol Blue) agar media. The nitrogen-fixing bacteria were analyzed with quantitative and qualitative methods. Six potential nitrogen-fixing isolates were identified based on the 16S rDNA sequence. The total number of isolates obtained from nitrogen-fixing isolation was six isolates. The R3.FN1 isolate showed the highest ammonia index at 0.52 µg.L-1 and was identified as Stenotrophomonas maltophilia KB13 with 99.87% similarity to the 16S rDNA sequence. Keywords: Cocoa, Nitrogen-fixing, Rhizosphere, Stenotrophomonas maltophilia.