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Ruminal Biohydrogenation Pattern of Poly-Unsaturated Fatty Acid as Influenced by Dietary Tannin Jayanegara, Anuraga
Indonesian Bulletin of Animal and Veterinary Sciences Vol 23, No 1 (2013)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (151.555 KB) | DOI: 10.14334/wartazoa.v23i1.954

Abstract

Abstract Large amounts of polyunsaturated fatty acids undergo transformation processes in the rumen through microbial biohydrogenation to form fatty acids with higher saturation degree. The respective process explains the high content of saturated fatty acids in products of ruminants and the potential risk of consumers’ health by consuming such products. Various nutritional approaches have been attempted to modulate biohydrogenation process in order to obtain healthier fatty acid profile from consumers’ perspective. The present paper is aimed to review the influence of dietary tannin, a naturally produced plant secondary compound, on the pattern of polyunsaturated fatty acids biohydrogenation occurring in the rumen. The effect of tannin on some key fatty acids involved in biohydrogenation process is presented together with the underlying mechanisms, particularly from up-to-date research results. Accordingly, different form of tannin as well as different level of the application are also discussed. Key words: Tannin, biohydrogenation, fatty acid, ruminant
Evaluation of Agro-Industrial by Products as Potential Local Feed for Ruminant Animals: Chemical Composition, Fiber Fractions and In Vitro Rumen Fermentation Pratama, Said Mirza; Wajizah, Sitti; Jayanegara, Anuraga; Samadi, Samadi
ANIMAL PRODUCTION Vol 20, No 3 (2018)
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (868.044 KB) | DOI: 10.20884/1.jap.2018.20.3.715

Abstract

Animals are considered as source of protein should be improved their productivity with the minimum cost production. Agro-industrial by products have been used as animal feed to reduce feed cost. The purpose of this study is to evaluate agro-industrial by product in Aceh as potential local feed for ruminant animals based on chemical composition, fiber fraction and in vitro rumen fermentation. There were eight sources of agro-industrial by products (sago residues, coconut meal, soybean-ketchup by product, coffee pulp, cacao pod, sago tree, corncob, and rice brand) which were collected from 3 different locations in Aceh.  All agro-industrial by product samples were dried at 600C for 24 h and ground to pass a 1 mm sieve. Grounded samples were analyzed to determine chemical composition, fiber fractions and in vitro rumen fermentation. Incubation was conducted at temperature 390C for 48 h in water bath with three replicates. Data for in vitro rumen fermentation were statically calculated by using SPSS differences between treatments were stated (P≤0.05) by using Duncan Multiple Range Test (DMRT). The results indicated that agro industrial by product from coconut meal, ketchup residues, coffee by product, cacao by product, and rice brand can be used as source of protein and industrial by product from sago by product, sago tree and corncob can be used as source of energy. Neutral detergent  insoluble  CP (NDICP) and Acid detergent insoluble CP (ADICP)  were relatively high for sago by product, sago tree and rice brand but relatively low for cacao by product and corncob. The value of incubated pH for most feed samples was in the normal range. In vitro dry matter digestibility (IVDMD) and in vitro organic matter  digestibility (IVOMD) were significantly difference (P≤0.05) each agro-industrial by products with the highest for sago and the lowest for coffee by product. In conclusion, agro-industrial by products had a potential feed for ruminant animals both as protein and energy sources. However, feed ingredients with high fiber content and low degradability, further treatments such as physical, chemical and biological treatments were required to improve the feed quality. 
Physiological Response and Haematological Profile of Reproductive Ewe Consuming Diet Supplemented with Black Tea Extract and Sunflower Seed Oil Diapari, Didid; Hermana, Widya; Prameswari, Febrina; Jayanegara, Anuraga
ANIMAL PRODUCTION Vol 19, No 3 (2017)
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (653.578 KB) | DOI: 10.20884/1.jap.2017.19.3.622

Abstract

This study was aimed to examine the effect of black tea extract addition in diet containing 4% and 6% sunflower oil on ewe physiological responses and blood profiles from late pregnancy until early lactation. This study was designed using a 2×2 factorial completely randomized design with 5 replications on 20 late-pregnant ewes. The first factor was two levels of sunflower seed oil (4% and 6%) and the second factor was the levels of black tea extract addition (0 ppm and 500 ppm). Data were analyzed by using analysis of variance (ANOVA) and Duncan’s multiple range test. Results showed that supplementation of sunflower seed oil and black tea extract had no effect on physiological responses and blood profiles of ewe during late pregnancy until early lactation except for erythrocyte numbers. Erythrocyte numbers were higher on 6% sunflower seed oil supplementation than that of 4% (P<0.05). In conclusion, addition of black tea extract in diets containing 4% and 6% sunflower seed oil limitedly affect physiological responses and blood profiles of ewe.
SUPPLEMENTARY FEEDING ON THE NUTRIENT BALANCE OF LACTATING DAIRY COW AT CONTRASTING TEMPERATURE REGIMES: ASSESSMENT USING CORNELL NET CARBOHYDRATE AND PROTEIN SYSTEM (CNCPS) MODEL Jayanegara, A.; Sofyan, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 34, No 3 (2009): (September)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.34.3.196-204

Abstract

Dairy cows often do not receive adequate nutrient supply during their lactation period. This condition caneven be worse if the environmental temperature is not in comfortable range which may occur especially intropical regions. The present research was aimed to simulate the effect of supplementary feeding on nutrientbalance of lactating dairy cow at contrasting temperature regimes using Cornell Net Carbohydrate andProtein System (CNCPS) model. Treatments consisted of feeds (R1: Pennisetum purpureum, R2: P.purpureum + concentrate (60:40), R3: P. purpureum + Gliricidia sepium + Leucaena leucocephala(60:20:20), R4: P. purpureum + concentrate + G. sepium + L. leucocephala (60:20:10:10)) and environmentaltemperatures (T1: 20 oC, T2: 30 oC). The dairy cow inputs in CNCPS were Holstein breed, body weight of500 kg, feed intake of 15 kg (dry matter basis) per day and produced milk 15 kg/day. Based on the CNCPSmodel, there were negative balances of metabolisable energy (ME) and metabolisable protein (MP) if alactating dairy cow fed only by P. purpureum. The ME balance was worse at higher temperature, while theMP balance was remain unchanged. Addition of concentrate mixture (R2) fulfilled the ME and MPrequirements as well as other nutrients. Addition of leguminous tree leaves (R3 and R4) improved thenutritional status of the lactating cow model compared to R1, but did not better than R2. It was concludedthat supplementary feeding is necessary for improving the nutrient balance of lactating dairy cow, especiallywhen the cow is maintained under uncomfortable environmental temperature.
THE USE OF PRINCIPAL COMPONENT ANALYSIS IN IDENTIFYING AND INTEGRATING VARIABLES RELATED TO FORAGE QUALITY AND METHANE PRODUCTION Jayanegara, A.; Makkar, H.P.S.; Becker, K.
Journal of the Indonesian Tropical Animal Agriculture Vol 34, No 4 (2009): (December)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.34.4.241-247

Abstract

This research was aimed to explore the use of multivariate statistics i.e. principal componentanalysis (PCA) in identifying and integrating variables related to forage quality and ruminal methaneproduction, and in classifying forage species into both characteristics. Seventeen plants were used as adatabase for the above mentioned purposes. Plant samples were determined for their chemicalcomposition, cumulative gas production (represents the nutrient degradation) and methane productionafter 24 hours of fermentation period using the Hohenheim gas test. The results showed that the PCAcould clearly identify factors related to forage quality and methane production and separated them intodifferent principal components (PC). The obtained PC1 was related to methane production andsubstantially influenced positively by crude protein, NDF, ADF (positive), total phenols, total tannins,condensed tannins and tannin activity (negative). On the other hand, the obtained PC2 was related tocumulative gas production (forage quality) and substantially influenced by crude protein (positive),NDF, ADF and condensed tannins (negative). Classification and screening of forages that have highquality and low methane production are possible using the PCA technique. Rhenum undulatum,Peltiphyllum peltatum and Rhus typhina were found to have such desired characteristics.
Nutritional comparison between dried and ensiled indigofera, papaya and moringa leaves Jayanegara, A.; Ardani, V.; Sukria, H. A.
Journal of the Indonesian Tropical Animal Agriculture Vol 44, No 1 (2019): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.44.1.77-83

Abstract

The objective of this experiment was to evaluate chemical composition, in vitro fermentation and digestibility of dried and ensiled indigofera, papaya and moringa leaves. The leaves were subjected to artificial drying in an oven at 60oC for 24 h and ensiling treatment for 30 d under room temperature. Dried and ensiled samples were determined for chemical composition, silage fermentation characteristics and in vitro rumen fermentation and digestibility. The experimental design was a factorial design 3 × 2 in which the first factor was different leaves (indigofera, papaya and moringa) and the second factor was conservation treatments (drying and ensiling). Determination of chemical composition was performed in duplicate whereas in vitro evaluation was conducted in three replicates. Results showed that ensiling treatment decreased CP contents of indigofera and moringa but not papaya leaves. Ensiling also decreased NDF and NDICP contents of all experimental leaves in comparison to drying treatment. The pH of all silages was high and they were characterized with high ammonia concentrations. Ensiled indigofera tended to have lower IVDMD and IVOMD as compared to dried indigofera (P<0.1). It can be concluded that ensiling of high protein forages leads to considerable extent of proteolysis.
Effect of napier grass supplemented with Gliricidia sepium, Sapindus rarak or Hibiscus rosa-sinensis on in vitro rumen fermentation profiles and methanogenesis Yuliana, P.; Laconi, E. B.; Jayanegara, A.; Achmadi, S. S.; Samsudin, A. A.
Journal of the Indonesian Tropical Animal Agriculture Vol 44, No 2 (2019): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.44.2.167-176

Abstract

This study examined the supplementation effects of gliricidia leaves(GL, Gliricidia sepium), lerak fruit(LF, Sapindus rarak), or hibiscusleaves(HL, Hibiscusrosa-sinensis) on in vitro rumen fermentation and methanogenesis and made a comparisonwith the Napier grass (NG, Pennisetum purpureum) grass. In vitro rumen fermentation was designed according to a randomized complete block design with four replications and seven treatments: NG, GL, LF, HL, NG 70% + GL 30%, NG 70% + LF 30%and NG 70% + HL 30%. The generated data were subjected to analysis of variance (ANOVA) with Duncan’s multiple range test and compared among treatment means.Addition of a plant containing saponin such as LF or HL to NG did not alter gas production after 24 and 48 h of incubation period in comparison to NG alone.The HLalone or blended with NGproduced the highest IVOMDduring the fermentation processas compared to other treatments (P<0.05). Adding LF supplement either singly or in combination significantly (P<0.05) reduced methane production in terms of %TVFA as compared to NG. Supplementation of LF plants has shifted VFA proportion towards more propionate and less acetate. The microbial population of LF, whether single or in combination had a tendency to reduce the rumen protozoa population but had no effect on methanogen population. It can be concluded that utilization of saponin-rich materials is particularly beneficial for reducing ruminal methane emission.
EXTRACTION OF TANNINS AND SAPONINS FROM PLANT SOURCES AND THEIR EFFECTS ON In vitro METHANOGENESIS AND RUMEN FERMENTATION Yuliana, P.; Laconi, E.B.; Wina, E.; Jayanegara, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 39, No 2 (2014)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.39.2.91-97

Abstract

This study was aimed to extract tannins from Swietenia mahagony and saponins from Sapindusrarak by using different solvents, and to test their extracts on in vitro rumen fermentation andmethanogenesis. Solvents used for extraction were water, methanol, acetone and their combinations.Tannin and saponin extracts were added into each incubation bottle containing Brachiaria humidicolagrass and Indigofera sp. legume (1:1 w/w) according to the following treatments (in four replicates): R1:control substrate, R2: R1 + 0.5 mg/ml tannin extract, R3: R1 + 1 mg/mL tannin extract, R4: R1 + 0.5mg/mL saponin extract, R5: R1 + 1 mg/mL saponin extract, R6: R1 + 0.5 mg/mL tannin extract + 0.5mg/mL saponin extract, and R7: R1 + 1 mg/mL tannin extract + 1 mg/mL saponin extract. Resultsrevealed that 75% water + 25% methanol was the best solvent to extract tannins from S. mahagonywhereas 100% methanol was the best to extract saponins from S. rarak. The highest gas production andthe lowest methane emission were obtained in R7. It can be concluded that combination of tannin andsaponin extracts were potential in mitigating ruminal methane emissions.
Effect of different transportation period on body weight loss, hematological and biochemical stress responses of sheep Lendrawati, L.; Priyanto, R.; Jayanegara, A.; Manalu, W.; Desrial, D.
Journal of the Indonesian Tropical Animal Agriculture Vol 45, No 2 (2020): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.45.2.115-123

Abstract

The study was conducted to evaluate the duration of road transportation during 4, 8 and 12 hours on body weight loss, hematological and biochemical responses of sheep. Twenty-four male Javanese thin tailed sheep were separated into transported group (T4, T8 and T12) and non-transported group (NT4, NT8 and NT12). Transportation (T) groups were transported for 4, 8 and 12 h in the same open pick up with 0.28 m2/head of loading density. Body weight, haematological and biochemical blood profiles were determined. The results showed that increasing of transportation duration significantly increased (P<0.05) body weight loss and decreased (P<0.05) hematocrit, cortisol and glucose values in the blood. However, these values were in the normal range of sheep well-being. In conclusion, transported healthy Javanese thin tailed sheep under good condition without feed and water access up to 12 h can tolerate to adverse effect of transportation without disturbing their welfare.
EVALUATION OF LACTICIN ADDITION ON GAS PRODUCTION KINETICS AND RUMEN METHANOGENESIS in Vitro Jayanegara, A.; Makkar, H.P.S.; Becker, K.
Journal of the Indonesian Tropical Animal Agriculture Vol 39, No 1 (2014): (March)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.39.1.30-36

Abstract

The present study was aimed to investigate the effect of a novel bacteriocin, i.e. lacticin 3147, ongas production kinetics and methane emission under in vitro rumen environment in two consecutiveexperiments. In experiment 1, either no or 10 µM of lacticin was added to hay or hay:concentrate (1:1,w/w) substrate. In experiment 2, the levels of lacticin additions were extended to 0, 10, 25 and 50 µM.Samples were incubated in three replicates in both experiments at 39oC for 24 h. Total gas productionand methane emission were measured during and after the incubation, respectively. Results revealedthat, in general, addition of lacticin had limited significant effects on gas production parameterscompared to control (without lacticin addition). Lacticin addition up to 50 µM did not significantlydecrease CH4 emission, although a tendency of methane reduction existed when lacticin was added at 25µM. Hay diet produced significantly less methane emission than that of hay:concentrate diet both inexperiment 1 and experiment 2 (P<0.05). It can be concluded that lacticin addition up to 50 µM wasunable to decrease CH4 emission in vitro and therefore need to be tested further at higher concentrations.
Co-Authors A S Tjakradidjaja A Sofyan A. A. Affan A. A. Samsudin A. A. Samsudin A. Anggraeny A. Atabany A. E. Suryani A. Fitri A. Ikhwanti A. Irawan A. Salleh A. Sofyan A. Sofyan A. Sofyan A. Sudarman A. T. Wahyudi A.K. Takyi A.O. Salih Achmadi, S. S. Afandi, Frendy Ahmad Ahmad Sofyan Ahmad Sofyan Allaily Tarman Anjas Asmara Samsudin Annisa Imran Ardani, V. Asep Sudarman Asep Sudarman Asep Sudarman Awistaros Angger Sakti Ayinda, R. S. K. Ayu Septi Anggraeni Aziz Kustiyo Budi Tangendjaja C. Hidayat D M Sinaga D. A. Astuti D. A. Astuti D. A. Astuti D. Setiawan D.A. Astuti DA Astuti DADIK PANTAYA Dedy Supriadin Desrial Desrial Desrial, D. DEWI APRI ASTUTI Dias Indrasti Didah Nur Faridah Didid Diapari, Didid E B Laconi E B Laconi E Palupi E Wina E. B. Laconi E. B. Laconi E. B. Laconi E. B. Laconi, E. B. E. Damayanti E. Wina E. Wina E.B. Laconi E.M. Hoffmann Elfawati Erika B. Laconi Eylen Desy Novita Gumilang Khairulli H. A. Hassim H. Herdian H. Julendra H. Matsui H. P S Makkar H.P S Makkar H.P.S. Makkar H.P.S. Makkar Hanny Hafiar Hari Agung Adrianto Hendra Herdian Hendra Herdian Heri Ahmad Sukria Hermana, Widya Heru Nugraha I G Permana I Komang Gede Wiryawan I. G. Permana I. Ikhsan Indah Wijayanti Irawan Sugoro Ismail Marzuki Ismail Marzuki Isnaini Dafri J. Nomura K Becker K. A. Fadhil K. A. Sarwono K. Becker K. Becker K. G. Wiryawan K. Nur Khasrad . Krisnan, Rantan L. Abdullah L. Abdullah L. Abdullah L. Khotijah Lendrawati Lendrawati Lendrawati, L. M. Abdelbagi M. Adhyatma M. Kondo M. M. Sholikin M. Makmur M. Zamri-Saad Milana Abdillah Subarkah Mira Andriani Mohamad Yamin Muhammad Arpah Muhammad Ridla Mukhtarom, Asrori Mulianda, R. N. Laylli Nahri Idris Nahrowi Nahrowi Nanang Krisnawan Nanang Krisnawan Novandri, B. Novia Qomariyah Nugraha Edhi Suyatma Nur Rochmah Kumalasari Nurmaya Rahmawati Muis Nurrul Suri Aulia Nurul Fitri Sari P. Yuliana Permana, I G Prameswari, Febrina Pratama, Said Mirza Pristian Yuliana Pudji Muljono Qomariyah, Novia R A Nurfitriani R P Harahap R Ridwan R. K. Rusli R. P. Harahap R. P. Harahap R. Priyanto R. Ridwan R. S. H. Martin R. Solfaine Rakhmad Perkasa Harahap Rantan Krisnan Retnani, Y Retnani, Yuli Retno Budi Lestari Retno Sri Hartati Mulyandari Rima Shidqiyya Hidayati Martin Rita Mutia Rizki Amalia Nurfitriani Rohmatussolihat Rohmatussolihat Roni Ridwan Roni Ridwan Roni Ridwan Rudi Priyanto Rusli Fidriyanto S. P. Dewi S. P. Dewi S. Suharti S. Suharti Sadarman Sadarman Sadarman Samadi Samadi Samsudin, A. A. Santi Ratnakomala Silitonga, Rhoito Frista Sitti Wajizah Sri Suharti Suci Wulandari Suharlina Suharlina Sukria, H. A. Sumiati Suminar Setiati Achmadi T Sutardi T. Ban-Tokuda T. M. Syahniar T. Obitsu T. Sabhan T. Suryati T. Toharmat Tangendjaja, Budi Theo Mahiseta Syahniar Toto Haryanto W. Manalu W. W. Wardani Wardani, W. W. Wasmen Manalu Win Ariga Bungsu Wina, E Wulansih Dwi Astuti Y Retnani Y. Marlida Y. Retnani Y. Yogianto, Y. Yantina, N. Yantyati Widyastuti Yantyati Widyastuti Yeni Widyawati Yeni Widyawati Yesi Chwenta Sari Yuli Retnani Zain, M.