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Umar Anggara Jenie
Lembaga Ilmu Pengetahuan Indonesia
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

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Impact of Curcuma mangga Val. Rhizome Essential Oil to p53, Bcl-2, H-Ras and Caspase-9 expression of Myeloma Cell Line Astuti, Endang; Sunarminingsih, Retno; Jenie, Umar Anggara; Mubarika, Sofia; S, Sismindari
Indonesian Journal of Biotechnology Vol 19, No 1 (2014)
Publisher : Universitas Gadjah Mada

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Abstract

Cancer is a disease, a public health problem, which is found in the world as well as in Indonesia. Ingeneral, some of cancer theraphies are ineffective, characterized by the resistance performance of cancer cell line,the exposed normal cell and by the side effects. Nowadays, studies to fi nd the specifi c and safely anti-cancerdrugs were increased by the time. Several studies revealed that Curcuma mangga Val. Rhizome contains somesecondary metabolites, essential or non-essential oil, which has cytotoxic activities to the cancer cells. Basedon these anti-cancer potentials, this study has several aims to recognize anti-cancer selectivity and molecularmechanism by inducting apoptosis and inhibiting myeloma cell proliferation. To C. mangga Val. essential oil,immunocyto chemical test was performed to determine the expression of p53, caspase-9, Bcl-2, H-Ras proteinwhile TUNEL test was performed to determine the number of apoptosis cells.The results of this study shown that anti-cancer molecular mechanism of C. mangga Val. essential oil tomyeloma cell line was performed by increasing apoptosis; by increasing the expression of pro-apoptosis p53,caspase-9 protein and reducing protein which is increasing proliferation Bcl-2 and H-Ras.
T47D cells arrested at G2M and Hyperploidy Formation Induced by a Curcumin’s Analogue PGV-1 Da’i, Muhammad; Jenie, Umar Anggara; AM, Supardjan; Kawaichi, Masashi; Meiyanto, Edy
Indonesian Journal of Biotechnology Vol 12, No 2 (2007)
Publisher : Universitas Gadjah Mada

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Abstract

its chemical structure than curcumin. As a curcumin analogue, PGV-1 was considered to have anticanceractivities. This research was conducted to study the effect of PGV-1 on the cycle progression of T47D cells. Cytotoxiceffects of PGV-1 on T47D cells were determined using MTT assay, and the the effect on cell cycle progressionwas carried out using flowcytometry. Western blot analysis was used to analyze protein expression correspondingto cell cycle progression. The result showed that at the concentration of 2.5 μM PGV-1 inhibited cell cycleprogression through G2/M arrest and induced of cells hyperploidy formation. The hyperploidy formation inducedby PGV-1 was related to the increase of cdc-2 expression. PGV-1 2.5 μM elevated the level of p21 CIP/KIPthrough p53- independent manner. Apoptosis was also induced by PGV-1 at early phase of treatment indicated byPARP cleavage due to activation of caspase-3/7 after 12 h treatment. The results above suggest that PGV-1 inhibitsthe growth of T47D cells targeted on microtubules.Keywords: PGV-1, G2/M arrest, apoptosis, p21
PENTAGAMAVUNON-1 MENGHAMBAT SIKLUS SEL T47D TERINDUKSI CASPASE INHIBITOR Z-VAD-Fmk PADA FASE G2-M Da’i, Muhammad; A.M., Supardjan; Jenie, Umar Anggara; M, Kawaichi; Meiyanto, Edy
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 5, No 4 (2011)
Publisher : Indonesian Research Gateway

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Abstract

Previous experiment indicated Curcumin’s analogue Pentagamavunon-1 (2,5-bis(41-hidroxy-31,51-dimethyl)-benzilidine-cyclopentanone) has inhibitory activity on T47D cell proliferation through induction of apoptosis and cell cycle arrest at G2-M phase. This research was conducted to observe the relationship between the induction of apoptosis and inhibition of cell cycle in T47D cells induced by Pentagamavunon-1 (PGV-1). The T47D cells were treated with 2.5 PGV-1 mM; Z-VAD-Fmk 2.5 mM; (Z-VAD-Fmk 2.5 mM+PGV-1 2.5 mM). Kinetics of cell proliferation was observed with flowcytometer analysis, molecular expression was observed with Western blot methods. The results showed induction of PGV-1 and Z-VAD-Fmk stimulate the accumulation of cells in G2-M phase (39.28%), did not differ significantly with cells that  induced by PGV-1 only (34.19%). Molecular analysis showed that treatment with (PGV-1+Z-VAD-Fmk) could prevent apoptosis through inhibition of activation of Caspase-3, increased the expression of p21 and activated Cdc-2. Overall the study showed inhibition of cell cycle at G2M phase by PGV-1 compound is not affected by the activation of caspase-3. ABSTRAK Analog kurkumin Pentagamavunon-1  (2,5-bis(41-hidroksi-31,51-dimetil)-benzilidinsiklo-pentanon) telah diteliti dapat menghambat proliferasi sel melalui mekanisme induksi apoptosis dan cell cycle arrest pada fase G2-M. Penelitian ini dilakukan untuk mengamati keterkaitan antara proses induksi apoptosis dan penghambatan siklus sel pada sel T47D yang diinduksi senyawa Pentagamavunon-1 (PGV-1). Sel T47D diberi perlakuan PGV-1 2,5 mM; Z-VAD-Fmk 2,5 mM dan (Z-VAD-Fmk 2,5 mM+PGV-1 2,5 mM). Kinetika proliferasi sel diamati dengan analisis flowcytometric, ekspresi molekuler diamati dengan metode Western blot. Hasil pengamatan menunjukkan induksi (PGV-1+Z-VAD-Fmk) memacu akumulasi sel pada fase G2-M (39,28%) tidak berbeda signifikan dengan sel yang hanya diinduksi PGV-1 (34,19%). Pengamatan molekuler menunjukkan perlakuan (PGV-1+Z-VAD-Fmk) mencegah terjadinya apoptosis melalui penghambatan aktivasi Caspase-3. Secara keseluruhan penelitian menunjukkan penghambatan siklus sel pada fase G2-M oleh senyawa PGV-1 tidak tergantung oleh aktivasi Caspase-3.
PENTAGAMAVUNON-1 MENGHAMBAT SIKLUS SEL T47D TERINDUKSI CASPASE INHIBITOR Z-VAD-Fmk PADA FASE G2-M Daâ??i, Muhammad; A.M., Supardjan; Jenie, Umar Anggara; M, Kawaichi; Meiyanto, Edy
Jurnal Farmasi Indonesia Vol 5, No 4 (2011)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35617/jfi.v5i4.54

Abstract

Previous experiment indicated Curcuminâ??s analogue Pentagamavunon-1 (2,5-bis(41-hidroxy-31,51-dimethyl)-benzilidine-cyclopentanone) has inhibitory activity on T47D cell proliferation through induction of apoptosis and cell cycle arrest at G2-M phase. This research was conducted to observe the relationship between the induction of apoptosis and inhibition of cell cycle in T47D cells induced by Pentagamavunon-1 (PGV-1). The T47D cells were treated with 2.5 PGV-1 mM; Z-VAD-Fmk 2.5 mM; (Z-VAD-Fmk 2.5 mM+PGV-1 2.5 mM). Kinetics of cell proliferation was observed with flowcytometer analysis, molecular expression was observed with Western blot methods. The results showed induction of PGV-1 and Z-VAD-Fmk stimulate the accumulation of cells in G2-M phase (39.28%), did not differ significantly with cells that  induced by PGV-1 only (34.19%). Molecular analysis showed that treatment with (PGV-1+Z-VAD-Fmk) could prevent apoptosis through inhibition of activation of Caspase-3, increased the expression of p21 and activated Cdc-2. Overall the study showed inhibition of cell cycle at G2M phase by PGV-1 compound is not affected by the activation of caspase-3. ABSTRAK Analog kurkumin Pentagamavunon-1  (2,5-bis(41-hidroksi-31,51-dimetil)-benzilidinsiklo-pentanon) telah diteliti dapat menghambat proliferasi sel melalui mekanisme induksi apoptosis dan cell cycle arrest pada fase G2-M. Penelitian ini dilakukan untuk mengamati keterkaitan antara proses induksi apoptosis dan penghambatan siklus sel pada sel T47D yang diinduksi senyawa Pentagamavunon-1 (PGV-1). Sel T47D diberi perlakuan PGV-1 2,5 mM; Z-VAD-Fmk 2,5 mM dan (Z-VAD-Fmk 2,5 mM+PGV-1 2,5 mM). Kinetika proliferasi sel diamati dengan analisis flowcytometric, ekspresi molekuler diamati dengan metode Western blot. Hasil pengamatan menunjukkan induksi (PGV-1+Z-VAD-Fmk) memacu akumulasi sel pada fase G2-M (39,28%) tidak berbeda signifikan dengan sel yang hanya diinduksi PGV-1 (34,19%). Pengamatan molekuler menunjukkan perlakuan (PGV-1+Z-VAD-Fmk) mencegah terjadinya apoptosis melalui penghambatan aktivasi Caspase-3. Secara keseluruhan penelitian menunjukkan penghambatan siklus sel pada fase G2-M oleh senyawa PGV-1 tidak tergantung oleh aktivasi Caspase-3.
Co-Authors Da'i, Muhammad Da’i, Muhammad Edy Meiyanto Endang Astuti M, Kawaichi M, Kawaichi Masashi Kawaichi, Masashi Muhammad Da'i Retno Sunarminingsih Sudibyo Sismindari . Sofia Mubarika H Supardjan A.M., Supardjan Supardjan AM, Supardjan