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All Journal Archive of Community Health METAMORFOSA Journal of Biological Sciences Buletin Udayana Mengabdi Jurnal Farmasi Udayana E-Journal Of Animal Science Udayana University Jurnal Veteriner Kesmas: Jurnal Kesehatan Masyarakat Nasional Jurnal Peternakan Indonesia (Indonesian Journal of Animal Science) Media Ilmiah Teknologi Pangan (Scientific Journal of Food Technology) Bali Anatomy Journal Interdisciplinary Social Studies Public Health and Preventive Medicine Archive
I Nengah Sujaya
Program Studi Ilmu Kesehatan Masyarakat Fakultas Kedokteran Universitas Udayana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Public Health Social Sciences Veterinary

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Eksopolisakarida dari Lactobacillus sp. Isolat Susu Kuda Sumbawa dan Potensinya sebagai Prebiotik (EXOPOLYSACCHARIDES FROM LACTOBACILLUS SP. ISOLATED FROM SUMBAWA MARE’S MILK AND ITS POTENTIAL APPLICATION AS PREBIOTICS) I Nengah Sujaya; Ni Putu Desy Aryantini; Ni Wayan Nursini; Cok. Istri Dewiyani Cakrawati; Ni Luh Made Ema Juliasari; Ni Made Utami Dwipayanti; Yan Ramona
Jurnal Veteriner Vol 13 No 2 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This research aimed to isolate exopolysaccharides (EPS) producing Lactobacilli isolated fromsumbawa mare’s milk and its potential as prebiotics for modulating the growth of Bifidobacteriumbreve. Nine strains of Lactobacillus sp. were screened for their capabilities to produce EPS usingmodified MRS medium containing sucrose. Prebiotics potential of the EPS was verified by culturingB. breve JCM1273 in TOS medium containing EPS. The results showed that all strains ofLactobacillus sp. produced EPS on MRS sucrose medium and two strains (Lactobacillus SK3.1 andLactobacillus SK4) produced more EPS compared to the other strains tested. Bifidobacteriumbreve JCM1273 showed weak activity while in direct metabolism of EPS produced by Lactobacillussp. SK4 and its growth was enhanced on acid hydrolyzed EPS. Since this phenomenon mighthappened when the EPS exposed by the low pH during gastric passage, hence the EPS might be apotential source to be developed as prebiotics. Nevertheless, further investigation is necessary toevaluate the bifidogenic affects of EPS in Lactobacillus sp. SK4.
CHARACTERIZATION OF LACTIC ACID BACTERIA ISOLATED FROM SUMBAWA MARE MILK Nengah Sujaya; Yan Ramona; Ni Putu Widarini; Ni Putu Suariani; Ni Made Utama Dwipayanti; Komang Ayu Nocianitri; Ni Wayan Nursini
Jurnal Veteriner Vol 9 No 2 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A study was carried out to isolate and characterize lactic acid bacteria (LAB) from the Sumbawa mares milk The Isolation of LAB was conducted in Man Rogosa Sharpe (MRS) agar. The isolates were characterized by standard methods, such as Gram staining, cell morphology study and fermentation activities. The ability of the isolates to inhibit some pathogenic bacteria was studied by dual culture assay. Isolates showing the widest spectrum of inhibiting pathogenic bacteria were further identified using API 50 CHL. The results showed that Sumbawa mare milk was dominated by lactobacilli and weisella/leuconostoc. As many as 26 out 36 isolates belong to homofermentative lactobacilli and another 10 isolates belong to both heterofermentative lactobacilli and weissella or leuconostoc. Twenty four isolates inhibited the growth of Escherichia coli 25922, Shigela flexneri, Salmonella typhimurium, and Staphylococcus aureus 29213. Two promising isolates with the widest spectrum of inhibiting pathogenic bacteria, Lactobacillus sp. SKG34 and Lactobacillus sp. SKG49, were identified respectively as Lactobacillus rhamnosus SKG34 and Lactobacillus ramnosus SKG49. These two isolates were specific strains of the sumbawa mare milk and are very potential to be developed as probiotic for human.
PROBIOTIC POTENCY OF LACTOBACILLUS SPP. ISOLATED FROM SUMBAWA MARE MILK I Nengah Sujaya; I Made Utami Dwipayanti; Ni Luh Putu Suariani; Ni Putu Widarini; Komang Ayu Nocianitri; Ni Wayan Nursini
Jurnal Veteriner Vol 9 No 1 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This research was deigned to elucidate the potency of Lactobacillus spp. isolated from sumbawa mare milk to be developed as a probiotic. Sixteen lacobacilli were screened based on their resitancy to a model of gastric juice at pH 2, 3, and 4, then followed by their resistncy to small intestional fluid model containing deoxycholic. Three lactobacilli i.e. Lactobacillus sp. SKA13, Lactobacillus rhamnosus SKG34 and Lactobacillus rhamnosus SKG49 were found to be resistentent to gastric juice at pH 3 and 4. However, there were no lactobacilli resisted to pH 2. Lactobacillus rhamnosus SKG34 and Lactobacillus rhamnosus SKG49 were able to reach the colon even after being expossed to a model of intestinal fluid containing 0,4 mM deoxycholate and pancreatine. Therefore, these isolates have a potency to be developed as probiotic lactobacilli. Nevertherless, these lactobcailli could probably transform cholic acid into secondary bile acids, which were not expected to be found in the probiotic, and this capability is not appropriate for probiotic. This character is worthly to be studied since it has never been reported in lactobacilli.
Front Cover & TOC Vol.8 No.3 I Nengah Sujaya
ARCHIVE OF COMMUNITY HEALTH Vol 8 No 3 (2021): Desember 2021
Publisher : Program Studi Sarjana Kesehatan Masyarakat Universitas Udayana Berasosiasi Dengan Ikatan Ahli Kesehatan Masyarakat Indonesia (IAKMI)

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INTRODUKSI PENGOLAHAN YOGURT DRINK DARI SUSU KAMBING DAN SARI BUAH NANAS DI DESA SEPANG BUSUNG BIU BULELENG I.N. Sujaya; K.A. Nocianitri; N.N.D. Fatmawati; N.W.A. Utami; I.D.P.K. Pratiwi; Y. Ramona
Buletin Udayana Mengabdi Vol 21 No 3 (2022): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Abstract

Susu kambing merupakan komoditas yang dihasilkan oleh Kelompok Peternak Kambing Tunas Mekar di Desa Sepang, Busungbiu, Buleleng. Komoditas ini berpotensi untuk meningkatkan kesejahteraan dan pendapatan masyarakat setempat. Namun, pemanfaatan susu kambing sebagai bahan baku produk olahan pangan masih terbatas. Tujuan pengabdian masyarakat ini adalah peningkatan diversifikasi olahan susu kambing untuk pemanfaatan hasil susu kambing pera peternak. Disamping masalah produksi yogurt, adanya aroma kambing pada produk yogurt yang dihasilkan mengganggu flavor yogurt susu kambing. Introduksi pengolahan susu kambing menjadi yogurt drink dengan penambahan sari buah nenas merupakan solusi yang ditawarkan untuk menutupi aroma kambing pada yogurt yang dihasilkan. Program ini dilaksanakan melalui peningkatan pengetahuan kelompok Wanita Tani (KWT) Sumber Rejeki sebagai masyarakat sasaran pengolah susu kambing dan peningkatan keterampilan melalui pelatihann pembuatan yogurt drink susu kambing. Kegiatan pengabdian telah berlangsung dengan baik dan tepat sasaran, terdapat 3 tahapan pelatihan yang dilaksanakan yang menjadi solusi bagi permaslahan mitra yaitu teknik pengolahan susu kambing menjadi yogurt, teknik pengolahan yogurt menjadi yogurt drink dengan memanfaatkan buah lokal yaitu nenas, teknik pengemasan dan pelabelan. Kegiatan pelatihan berhasil dengan baik dengan adanya respon masyarakat yang sangat positif yang terlihat dari keaktifan peserta selama diskusi dan selama pelatihan berlangsung. Melalui kegiatan ini, kelompok peternak Tunas Mekar dan KWT Sumber Rejeki telah mampu memproduksi yogurt dari susu kambing dan yogurt drink sehingga dapat meningkatkan nilai tambah dari susu kambing.
Isolasi dan Idetifikasi Bakteri aam Laktat Penghasil Bakteriosin dari Urutan, Sosis Fermentasi Tradisional Bali Ni Made Sri Dwijastuti; I Nengah Sujaya; Ni Nengah Dwi Fatmawati
Metamorfosa: Journal of Biological Sciences Vol 8 No 1 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i01.p08

Abstract

This study aimed to isolate and identify lactic acid bacteria (LAB) that could produce bacteriocins from urutan. Urutan is a Balinese sausage that is traditionally produced and naturally fermented. Urutans used in this study were obtained from several traders in the Baturiti, Mengwi, and Tegallalang region, Bali. More than 300 LAB colonies were isolated and underwent inhibition test against the growth of the indicator bacteria. Crude bacteriocins from two isolates, namely J2 and J6, showed inhibition zones against the growth of Lactobacillus plantarum E12.1 and Escherichia coli ATCC 8739. The J6 isolate also inhibited the growth of Enterococcus faecalis ATCC 29212. Both J2 and J6 were gram-positive, bacilli shaped, and showed negative catalase test. Based on the results of the 16S rDNA gene amplification, the two isolates were identified as Lactobacillus plantarum. Keywords: Lactic Acid Bacteria, Bacteriocin, Lactobacillus plantarum, Urutan
Isolasi dan Identifikasi Bakteri Asam Laktat dari Cairan Rumen Sapi Bali sebagai Kandidat Biopreservatif ISOLATION AND IDENTIFICATION OF ACID LACTIC BACTERIA FROM BALI CATTLE’S GASTRIC FLUID AS A POTENTIAL CANDIDATE OF BIOPRESERVATIVE I Wayan Suardana; I Nyoman Suarsana; I Nengah Sujaya; Komang Gede Wiryawan
Jurnal Veteriner Vol 8 No 4 (2007)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A study was conducted to isolate and identify of lactic acid bacteria originated from gastric fluid of bali cattle, and to determine their potential as the candidates of biopreservative. Lactic acid bacteria were isolated by culturing the gastric fluid of bali cattle in de Mann, Rogosa, Sharpe (MRS) medium; screening the bacteria, and identification of bacteria species by Analytical Profile Index (API) 50 CHL Kit. The results showed that, the new species of lactic acid bacteria were isolated and identified as Lactococcus lactis spp lactis 1 (SR21 isolate) and Lactobacillus brevis 1 (SR54 isolate) that have broad spectrum antimicrobial activities. It is clear from this study that a potential lactic acid bacteria producing antimicrobial agent can be isolated from the gastric fluid of bali cattle.
ENERGY BALANCE AND PERFORMANCE OF BALI CATTLE FED WITH DIFFERENT TYPES AND COMPOSITIONS OF FORAGE Suryani, N. N.; Mahardika, I G.; Putra, S.; Sujaya, N.
E-Journal Of Animal Science Udayana University Vol 1, No 1, Tahun 2012
Publisher : E-Journal of Animal Science Udayana University

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Abstract

An experiment was carried out to determine the energy balance and performance of bali cattle fed different forage compositions. Randomized Block Design consisted of four feed treatments with 3 block of weight live as replicates were used in this study. The initial weight of male bali cattle used range between 181-265 kg. These four treatments based on dry matter (DM) were: A (45% elephant grass + 0% rice straw + 15% glyricidia + 10% calliandra + 30% concentrate); B (30% elephant grass +10% rice straw + 20% glyricidia + 10% calliandra+ 30% concentrate) ; C (15% elephant grass +20% rice straw + 25% glyricidia +10% calliandra + 30% concentrate)  and treatmen D (0% elephant grass + 30% rice straw + 30% glyricidia  + 10% calliandra+ 30% concentrate) . Variables which were measured: nutrient consumption, balance energy, energy retention, weight gain and FCR. The results showed that Dry Matter and energy intake were highest in treatment C, but not significantly different (P>0.05) compare with the others. Weight gain was significantly the highest (P<0.05) in treatment C and FCR significantly the lowest (P<0.05). Energy retention treatments B, C and D did not show significant differences (P> 0.05) but in the A treatment significantly the lowest (P<0.05). Heat production significantly the lowest (P<0.05) in treatment D compared to treatment A and C. It could be concluded that the ration C gave lowest FCR and had higest weight gain compared to others treatment.
Identifikasi Penyebab Diare di Kabupaten Karangasem, Bali I N. Sujaya; N.P. Desy Aryantini; N.W. Nursini; S.G. Purnama; N.M.U. Dwipayanti; I G. Artawan; I M. Sutarga
Jurnal Kesehatan Masyarakat Nasional Vol. 4 No. 4 Februari 2010
Publisher : Faculty of Public Health Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (229.638 KB) | DOI: 10.21109/kesmas.v4i4.180

Abstract

Pada Februari hingga Maret 2008 terjadi kejadian luar biasa muntah berak (diare) di Kabupaten Karangasem Bali. Tercatat sekitar 600 orang mengalami muntaber dan 5 orang meninggal dunia. Ini merupakan kejadian KLB muntaber pertama kali di Bali serta belum diketahui patogen penyebab diare tersebut. Tujuan penelitian ini adalah mengidentifiaksi penyebab diare di Karangasem serta kemungkinan rantai penularannya. Penelusuran penyebab KLB dilakukan denganmenganalisis sampel air yang diambil dari sumber air umum, cubang/sumur penduduk, bahan makanan, serta rectal swab penderita dengan kombinasi tek-nik pemupukan kuman dan PCR spesifik dengan target gen pembentuk toksin pada Escherichia coli. Dengan melakukan kultur pada sampel makanan diperoleh bahwa 11 dari 21 sampel makanan positif mengandung E. coli. Dari sampel yang positif E. coli, 2 sampel yang diambil di rumah penderita muntaber ter-deteksi gen pembentuk shiga like toxin tipe I dan II pada E. coli. Deteksi gen pengkode shiga like toxin tipe I juga terdeteksi pada penderita dan beberapa sampel air dari cubang penduduk. Hal ini menunjukkan bahwa E. coli pembentuk shiga ike toxin tipe I merupakan penyebab KLB di Karangasem. Lebih lan-jut diperoleh bahwa pita shiga like toxin tipe I dan tipe II. E. coli strain Karangasem berbeda dengan strain EHEC sehingga strain Karangasem ini kemungkinan merupakan strain E. coli patogen baru yang terjadi akibat perubahan genetik pada E. coli pembentuk shiga like toxin yang ditemukan di Bali. Kata kunci : Diare, Escherichia coli, shiga like toxinAbstractIn February to March 2008, Bali was shocked by the outbreak of diarrhea in Karangasem District, Bali. It was recorded that 600 people were having diarrhea and 5 people were died due to the disease. This outbreak was the first time happened in Bali and the causing pathogen was not yet identified. The aim of this study was to identify the causing pathogen of diarrhea in the outbreak case in Karangasem, as well as to identify the possible transmitting pathway. The tracking of outbreak cause was carried out by analyzing water sample taken from communal clean water source, private clean water reservoir, food sample, as well as rectal swab of the patient with the combination of pathogen enrichment technique and specific PCR with Escherichia coli as the target of toxin forming agent. Based on the culture growth from food samples, it was found that 11 from 21 samples were E. colipositive. From samples that E. colipositive, 2 sam-ples that were taken from patient’s house were detected a shiga like toxic forming gene, type I and II on the E. coli. The similar shiga like toxin forming genetype I was also detected on samples from patient and samples from water of private family cubang. This shows that E. colithat forms shiga like toxin type I was the diarrhea causing pathogen in this particular outbreak in Karangasem. Furthermore, it was found out that the ribbon formed by shiga like toxin type I and II differ from the strain of EHEC. Thus, it is possible that the strain found in Karangasem was a new strain of E. colipathogen due to genetic transformation on shiga like toxin forming E. colithat was found in Bali. Key words : Diarrhea, Escherichia coli , shiga like toxin
PENGEMBANGAN STARTER DARI Lactobacillus spp. ISOLAT SUSU KUDA SUMBAWA UNTUK PEMBUATAN SUSU TERFERMENTASI I Gusti Ayu Kusuma Dewi; I Gusti Ngurah Agung Dewantara Putra; I Nengah Sujaya
Jurnal Farmasi Udayana Vol. 2, No. 1, Tahun 2013
Publisher : Departement of Pharmacy, Faculty of Mathematics and Natural Science, Udayana University

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Abstract

Susu terfermentasi yang beredar di pasaran menggunakan bakteri probiotik yang bukan berasal dari strain lokal Indonesia. Beberapa strain yang diisolasi dari susu kuda sumbawa menunjukkan potensi untuk dikembangkan menjadi probiotik potensial. Namun, sebagian besar strain-strain belum diuji kemampuannya dalam proses karakterisasi susu untuk membuat susu terfermentasi. Penelitian ini bertujuan untuk menggali potensi Lactobacillus spp. isolat susu kuda Sumbawa sebagai starter susu terfermentasi. Sebanyak 32 isolat Lactobacillus spp. isolat susu kuda Sumbawa yang berpotensi sebagai probiotik diseleksi kemampuannya untuk dikembangkan sebagai starter susu fermentasi yang didasarkan pada produksi massa sel, pH dan karakteristik organoleptik susu terfermentasi. Dari 32 isolat Lactobacillus spp. yang dipergunakan diperoleh 2 isolat potensial yaitu Lactobacillus sp. SMM37 dan Lactobacillus sp. SMM42. Kedua isolat ini dapat memproduksi massa sel yang cepat, menghasilkan susu terfermentasi dengan pH yang baik yaitu Lactobacillus sp. SMM37 sebesar 3,68 dan Lactobacillus sp. SMM42 sebesar 4,19, serta karakteristik organoleptik yang baik yang meliputi rasa, kekentalan (tekstur), ada tidaknya butiran (curd).
Co-Authors Cahyawati, Putu Nita Cok. Istri Dewiyani Cakrawati Fukuda Kenji I D.P. Kartika Pratiwi I Dewa Made Sukrama I g. H. Purnama I Gede Mahardika I Gusti Ayu Kusuma Dewi I Gusti Ngurah Agung Dewantara Putra I Komang Gede Wiryawan I Made Agus Gelgel Wirasuta I MADE SUTARGA I Nyoman Suarsana I Wayan Gede Artawan Eka Putra I Wayan Suardana I.D.P.K. Pratiwi Ida Ayu Sri Sinca Maha Uni K.A. Nocianitri Kadek Tresna Adhi KOMANG AYU NOCIANITRI Levi Anatolia S.M. Exposto Luh Aris Aryandeni Utami Luh Putu Lila Wulandari Made Ayu Hita Pretiwi Suryadhi N T. Suryadhi N. N. Suryani N. T. Suryadhi N.P. Desy Aryantini N.W. Nursini N.W.A. Utami Ni Ketut Sutiari Ni Luh Made Ema Juliasari Ni Luh Putu Suariani Ni Made Sri Dwijastuti Ni Made Utami Dwipayanti Ni Nengah Dwi Fatmawati Ni Nyoman Suryani Ni Putu Desy Aryantini Ni Putu Eka Trisdayanti Ni Putu Suariani Ni Putu Wiastari Ni Putu Widarini Ni Wayan Nursini Putu Rima Sintyadewi S. G. Purnama S. Putra S. PUTRA sang gede purnama Sawitri, Anak Agung Sagung Sentana Putra Tadashu Urashima Wayan Redi Aryanta Wayan Tunas Artama Yan Ramona Yoshitake Orikasa Yuji Oda