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All Journal METAMORFOSA Journal of Biological Sciences Bumi Lestari Biogenesis: Jurnal Ilmiah Biologi Jurnal Ilmiah Perikanan dan Kelautan The Indonesian Biomedical Journal Jurnal Biologi Tropis Jurnal Medik Veteriner Aquacultura Indonesiana Journal of Aquaculture and Fish Health Indian Journal of Forensic Medicine & Toxicology Jurnal Ilmiah Sains Seminar Ilmiah Nasional Teknologi, Sains, dan Sosial Humaniora (SINTESA) Jurnal aplikasi IPTEK
Putu Angga Wiradana
Program Studi Biologi, Fakultas Ilmu Kesehatan, Sains, Dan Teknologi, Universitas Dhyana Pura
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Earth & Planetary Sciences Education Energy Environmental Science Health Professions Immunology & microbiology Mathematics Physics Public Health Social Sciences Veterinary

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Biomonitoring Escherichia coli and Coliform Contamination in Abalone (Haliotis squamata) Cultivation Pond in Musi Village, Gerokgak Sub-District, Buleleng-Bali. Wiradana, Putu Angga; Yusup, Deny Suhernawan; Soegianto, Agoes
Aquacultura Indonesiana Vol 20, No 1 (2019)
Publisher : Indonesian Aquaculture Society (MAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (357.013 KB) | DOI: 10.21534/ai.v20i1.143

Abstract

Abalone (Haliotis squamata) is one of the fisheries commodities that have high economic value. This study was conducted to investigate contamination of Escherichia coli and coliform in Abalone cultivation ponds in the Musi Village area, Buleleng Regency, Bali. An experiment was carried out by taking water samples in the abalone pond every week for one month. The results showed that there was no difference between the total number of Escherichia coli and coliform. Water samples that were positive for coliform in the determination test had no differences in each sample with total coliform values (Colonies / 100mL) of 4, 3, 7 and 9 (MPN / 100mL) respectively. While the total number of Escherichia coli is shown after being grown in EMBA selective media which are 4, 3, 7, 4 (MPN / 100mL) respectively. Meanwhile, when compared with the number referenced from the Decree of the State Minister of Environment Number 51 of 2004 concerning Sea Water Quality Standards, the quality of seawater for aquatic cultivation in this study is still classified as Safe (<1000 MPN / 100mL).
Steroidogenesis Mechanism, Disruption Factor, Gene Function, and Role in Male Fertility : A Mini Review I Gede Widhiantara; Anak Agung Ayu Putri Permatasari; I Wayan Rosiana; Putu Angga Wiradana; BagusKomang Satriyasa
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 4 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i4.16914

Abstract

Male fertility can be defined as the ability of the male reproductive system to impregnate a woman, whileinfertility is the condition of a married couple who have been married for one year or more and have had sexualintercourse regularly or without using contraception but do not have a pregnancy or offspring. About 10% ofmarried couples experience infertility. The main organ of male reproduction is the testes because in the testesthe process of forming spermatozoa and the hormone testosterone occurs. The hormone testosterone plays adirect role in the continuity of spermatogenesis. Testosterone is produced through a series of steroidogenesismechanisms in testicular Leydig cells. Several factors influence the course of steroidogenesis such as Leydigcells, steroidogenesis proteins, related genes to the influence of free radicals. These factors are closelyrelated to diet and lifestyle. This study is important to understand in efforts to prevent infertility in men.
Evaluation of antimicrobial activity and phytochemical screening of red Kamboja (Plumeria rubra L.) extracts Ni Kadek Yunita Sari; Ni Wayan Deswiniyanti; Putu Angga Wiradana
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 2 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i2.25409

Abstract

Natural antimicrobial sources such as red Kamboja (Plumeria rubra L.) flower extract can be utilized to treat infectious disorders caused by Staphylococcus aureus, Escherichia coli, and Candida albicans. This study aims to determine evaluate the effectiveness of methanol and ethanol extracts of P. rubra floral against E. coli, S. aureus, and C. albicans growth, as well as the amount of secondary metabolites in P. rubra extract. The study's findings indicate that the highest DIZ value of P. rubra methanol extract was 7.40 mm, 7.36 mm, and 7.30 mm for S. aureus ATCC25923 at 5%, 10%, and 20%, respectively, while the highest DIZ value for C. albicans ATCC10231 at 10%, 10%, and 20% was 25.08 mm, and 25.04 mm, respectively. The DIZ value of the P. rubra flower ethanol extract against E. coli strain was 5.26 mm at 5%, and 7.30 mm at 20%. Secondary metabolites of saponins, tannins, alkaloids, flavonoids, steroids, and phenols were present in the methanol and ethanol extracts of P. rubra flowers. In summary, our findings highlight the use of P. rubra flower extract as a biological source with antibacterial properties for the control of human infectious illnesses.
Komposisi Floristik Vegetasi di Kawasan Hilir Sungai Unda, Kabupaten Klungkung, Bali I Made Sara Wijana; I Made Saka Wijaya; Abd. Rahman As-syakur; Gede Surya Indrawan; Putu Angga Wiradana; Komang Kartika Indi Swari; Dewa Ayu Intan Tirta Sari
Metamorfosa: Journal of Biological Sciences Vol 9 No 1 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i01.p03

Abstract

The downstream area of Unda River Watershed located in Klungkung Regency has experienced succession due to the eruption of Mount Agung in 1963. The ecosystem in the downstream area of the Unda River also experienced anthropogenic succession due to human activities which affected the plant species that composing the vegetation. This study aims to observe the floristic composition of vegetation in the downstream area of the Unda River. The research was conducted by exploration method in three types of ecosystems: lowland forest, marshes, and beaches. The results show the floristic composition in the downstream of Unda River composed by 163 plant species, classified in 48 families and 128 genera. The major growth form is small herb (47 species), grass (35 species), and tree (31 species). Fabaceae is the plant family with the most abundant species, followed by Poaceae and Cyperaceae. Based on the research zones, the lowland forest has the highest number of plant species (120 species), while the beach zone has the lowest (82 species). The ecosystem stability in the downstream area of Unda River can be disturbed by the existence of invasive species, such as Eichhornia crassipes, Chromolaena odorata, Lantana camara, Ipomoea carnea, Vachellia farnesiana, and Typha angustifolia.
Checklist, Indeks Ekologi, dan Status Konservasi Komunitas Fauna Akuatik di Kawasan Sungai Unda dan Sekitar Pantai Jumpai, Kabupaten Klungkung, Provinsi Bali Gede Surya Indrawan; Putu Angga Wiradana; I Made Saka Wijaya; Abd. Rahman As-syakur; M. Rheza Rizki Syahputra; I Made Sara Wijana
Bumi Lestari Journal of Environment Vol 21 No 1 (2021)
Publisher : Environmental Research Center (PPLH) of Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/blje.2021.v21.i01.p02

Abstract

Aquatic organisms or nekton have an important role in supporting the stability of the aquatic environment and human life. This study aims to determine the nekton type community, ecological index, and their conservation status in the Unda River area and around Jumpai Beach, Klungkung Regency, Bali. The study was conducted in July - August 2020. The study was conducted in 2 communities, namely Macrozoobenthos and Nekton. Macrozoobenthos samples were collected using the quadratic method in the Jumpai Beach area and the direct observation method along the Unda River. Nekton communities are collected through primary data using "survey methods" and secondary data through interviews with fishermen and local communities. The ecological index data were analyzed using the Shannon-Weiner diversity index (H'), dominance index (C), and uniformity index (E), while the data on Nekton type and conservation status were analyzed descriptively. The nekton species community in the waters of the Unda River and Jumpai Beach areas was obtained as many as 26 species in 21 families which were dominated by Kepala Timah (Aplocheilus panchax) and sepat rawa (Trichogaster trichopterus), while Nautica sp for the Macrozoobenthos community. The ecological index of the aquatic community is the Shannon-Weiner Diversity Index (H') value of 2.98 (moderate), the wealth index (R) of 3.95 (moderate), and the evenness index (E) of 1.21. (High). The highest percentage ratio of nekton communities was obtained by the Macrozoobenthos community, namely 53%, while the nekton species was 46%. Conservation status shows that 13 species are included in the LC (Least Concern) category, 12 species are included in the NE (Not Evaluated) category, and 1 species are included in the VU (Vulnerable) category.
Antihypercholesterolemic and Antioxidant Effects of Blumea balsamifera L. Leaf Extracts to Maintain Luteinizing Hormone Secretion in Rats Induced by High-Cholesterol Diets I Gede Widhiantara; Anak Agung Ayu Putri Permatasari; I Wayan Rosiana; Putu Angga Wiradana; Luh Putu Widiastini; I Made Jawi
The Indonesian Biomedical Journal Vol 13, No 4 (2021)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v13i4.1694

Abstract

BACKGROUND: The discovery of herbal ingredients for antihypercholesterolemic and to improve male reproductive function is very necessary due to high-cholesterol diet factors. Blumea balsamifera leaf extract (BBLE) is known to be able to increase the number and diameter of Leydig cells in rats given high-fat feed. This study was to conducted to determine the levels of total cholesterol, body weight, plasma malondialdehyde (MDA), Superoxide Dismutase (SOD), Luteinizing Hormone (LH) in high-cholesterol-fed male rats provided with BBLE.METHODS: This research utilized a randomized post-test only control group. Ethanol was used to extract the BBLE, which was then evaporated. For 21 days, 16 Wistar rats were given a high-cholesterol diet. To determine the effect of BBLE on the high-cholesterol diet, the samples were divided into two groups (control and BBLE group) on day 22. The treatments lasted 30 days. SOD, plasma MDA, LH, and total cholesterol were measured.RESULTS: The results showed that the SOD and LH parameters were significantly higher in the treatment of BBLE compared to the control group (p<0.05). The parameters of total cholesterol levels, bodyweight, and MDA of rats given BBLE were significantly lower than those of the control group (p<0.05).CONCLUSION: Our findings highlight that BBLE has antihypercholesterolemic and antioxidant effects. The BBLE also has potential to be used as a therapy to maintain male reproductive function because it has a positive effect on the hypothalamic-pituitary axis through increasing LH secretion.KEYWORDS: Malondialdehyde, Superoxide dismutase, Luteinizing hormone, Blumea balsamifera extract, High-cholesterol diet
Status, Trends, and Potentials of Turtle Conservation in Bali: A Mini Review Yesha Ainesis El Gracianita Pelupessy; Putu Angga Wiradana; I Wayan Rosiana; I Gede Widhiantara
Jurnal Medik Veteriner Vol. 4 No. 2 (2021): October
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss2.2021.256-268

Abstract

Sea turtles in Bali has been exploited excessively, which caused decrease in population, and this issue has placed sea turtles threatened to extinction. This review article aimed to provide information regarding the status, trends, and potential of sea turtles in Bali. Information given in this article is vital to ensure reliable knowledge not to only understand our current situation, but also to increase efficiency in reliant to the problem sea turtles face. The literature study method is used to write this review paper, namely by accessing a number of research paper published nationally and internationally. It was explained that the status of sea turtles is known to be endangered and law enforcement is not sufficient overcoming the problem. Conservation trends such as nurturing hatchlings have shown to not only help restore the population of sea turtles, but also to bring economic benefits to the conservation sites and the people. Potentials of ecotourism and DNA Barcoding has shown to be effective to benefit the people economically and increase the efficiency of law enforcement and conservation. Solutions and methods of improvement such as ecotourism and DNA Barcoding explained in this article is practical for Bali to adapt, so that sea turtle conservation is capable to overcome its status and incline to its potential.
First Report on Molecular Identification of Caulerpa Green Algae from Mandangin Island Indonesia Using Partial 18SrRNA Genes Nwet Darli Kyaw Zaw; Putu Angga Wiradana; Sin War Naw; Aondohemba Samuel Nege; Mochammad Amin Alamsjah; Rizhar Eman Karunia Akbar; Fahror Rosi
Journal of Aquaculture and Fish Health Vol. 9 No. 3 (2020): JAFH Vol. 9 No. 3 September 2020
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v9i3.19255

Abstract

Caulerpa is one of the seaweed that grows naturally in Indonesian waters such as those in Mandangin Island. This study aimed to identify Caulerpa sp. based on molecular analysis using certain genetic markers. This research is expected to provide information on the identification of macroalgae from Indonesia waters, especially Mandangin Island, Madura with the use of molecular analysis based on 18SrRNA primers. The two green seaweed samples from the Caulerpa genus in this study were successfully analyzed using 18SrRNA primers. The BLAST results of samples 1and 2 are related to Caulerpa taxifolia 18SrRNA, but in the phylogenetic tree result, Sample 1 was more closely related to Caulerpa sertularioides f. longipes. 18SrRNA primers have been used for molecular identification of green seaweed from Mandangin for the first time and this shows that barcode markers can be used for molecular identification of seaweed, specifically Caulerpa in the waters of Mandangin Island, Indonesia.
Supplementation of Lysine Essential Amino Acids in Commercial Feed to Increase Eicosapentaenoic Acid (EPA) and Docosahexaenoic Acid (DHA) in Catfish (Pangasius sp.) Fillet Muhammad Taufiq Shidqi; Agustono Agustono; Mirni Lamid; Putu Angga Wiradana
Jurnal Biologi Tropis Vol. 21 No. 3 (2021): September - Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v21i3.2995

Abstract

The purpose of this study is to see how adding the amino acid lysine to commercial feed affects the amount of EPA and DHA in catfish (Pangasius sp.) flesh. This study is an experimental study with a totally randomized design that includes four treatments and five replications. P0 (100% commercial feed + 1.2 percent amino acid lysine), P2 (100% commercial feed + 2.2 percent amino acid lysine), and P3 (100% commercial feed + 3.2 percent amino acid lysine) were the treatments. The parameters observed were EPA and DHA in catfish meat. Data analysis used ANOVA and continued with the DUNCAN test (p 0.05). The results showed that the addition of the amino acid lysine to commercial feed for 30 days of maintenance was able to increase the EPA and DHA content in catfish meat. The highest EPA content was obtained by treatment P1 (1.2% amino acid lysine), which was 0.597%, while the highest DHA content was obtained by treatment P2 (2.2% amino acid lysine), which was 0.747%. Our findings demonstrate that adding the amino acid lysine to the diet can result in nutrient-rich catfish flesh. However, studies on the influence of lysine amino acid on the growth rate, survival rate, and cholesterol ratio in catfish flesh are predicted to enhance the productivity of this very nutritious fish.
Struktur dan Komposisi Vegetasi pada Suksesi di Muara Sungai Unda, Kabupaten Klungkung, Bali I Made Saka Wijaya; Gede Surya Indrawan; Putu Angga Wiradana; I Made Sara Wijana; Abd. Rahman As-syakur; Adi Ariyanto Wibisono; Viryanando Evan Rahardja
JURNAL ILMIAH SAINS Volume 21 Nomor 1, April 2021
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1094.862 KB) | DOI: 10.35799/jis.21.1.2021.31744

Abstract

 Letusan Gunung Agung pada tahun 1963 memberikan dampak ekologis di sekitar muara Sungai Unda, Kabupaten Klungkung, Bali. Lahar yang mengalir melalui sungai tersebut menyebabkan terjadinya suksesi primer akibat tutupan pasir dan batu. Selama 57 tahun, muara Sungai Unda tidak hanya mengalami suksesi alam, tetapi juga mengalami suksesi antropogenik melalui pemanfaatan sebagai tambang pasir, lahan pastura, dan permukiman sementara.  Penelitian ini bertujuan untuk mempelajari struktur dan komposisi vegetasi pada suksesi di muara Sungai Unda. Penentuan struktur vegetasi dilakukan pada 8 stasiun penelitian dengan menggunakan metode plot dengan jumlah 24 plot pohon (20 x 20 m) dan 48 plot vegetasi lantai (1 x 1 m). Data dianalisis menggunakan parameter ekologis seperti densitas, densitas relatif, frekuensi, frekuensi relatif, dominansi, dominansi relatif, dan nilai penting, serta dilengkapi dengan indeks keragaman Shannon-Weiner (H’), indeks dominansi (C), dan indeks keseragaman (E). Hasil penelitian menunjukkan bahwa semak dan rumput merupakan bentuk hidup yang paling menentukan struktur vegetasi di Muara Sungai Unda. Beberapa spesies penyusun vegetasi tersebut merupakan tumbuhan invasif seperti yang harus dikontrol populasinya adalah Typha angustifolia, Ipomoea carnea, Chromolaena odorata, Lantana camara, dan Vachellia farnesiana. Berdasarkan indeks yang digunakan, keanekaragaman vegetasi termasuk dalam kategori tinggi, tanpa ada jenis yang mendominasi, dengan persebaran yang merata.Kata kunci: Gunung Agung; spesies invasive; suksesi antropogenik; vegetasi suksesiStructure and Composition of Successional Vegetation in Unda River, Klungkung Regency, BaliABSTRACTMount Agung eruption in 1963 caused major ecological impact around Unda River, Klungkung Regency, Bali. The volcanic mudflow that surpass the Unda River watershed initiate the primary succession in those areas. This research aimed to study the structure and composition of successional vegetation in Unda River. Twenty-four tree plots (20 x 20 m) and 48 understorey plots (1 x 1 m) derived from 8 research stations were used to determine the structure of vegetation. The data analyzed by ecological parameters, such as density, relative density, frequency, relative frequency, dominancy, relative dominancy, and importance value, then completed with Shannon-Weiner diversity index (H’), dominancy index (C), and evenness index (E). The results shown that the vegetation structure in Unda River determined by two major growth form: shrubs and grasses. Some notable invasive species that should be controlled are Typha angustifolia, Ipomoea carnea, Chromolaena odorata, Lantana camara, and Vachellia farnesiana. Based on the three indexes, the vegetation diversity was categorized as high diversity, without any species domination, and the distribution of the species are uniformKeywords: Anthropogenic succession; invasive species; Mount Agung; successional vegetation
Co-Authors Abd. Rahman As-syakur Adi Ariyanto Wibisono Agoes Soegianto Agoes Soegianto Agung Wahyu Permadi Agustono Agustono Anak Agung Ayu Putri Permatasari Aondohemba Samuel Nege Bagus Komang Satriyasa Bambang Yulianto Deny Suhermawan Yusup Dewa Ayu Intan Tirta Sari Fahror Rosi Gede Surya Indrawan I Gede Widhiantara I Made Gde Sudyadnyana Sandhika I Made Jawi I Made Saka Wijaya I Made Sara Wijana I Made Sara Wijana I Wayan Rosiana Komang Kartika Indi Swari Luh Putu Widiastini M. Rheza Rizki Syahputra Matius Victorino Ola Dame Mirni Lamid Muhammad Taufiq Shidqi NI KADEK YUNITA SARI Ni Komang Widiastuti Ni Wayan Deswiniyanti Nwet Darli Kyaw Zaw Permatasari, Anak Agung Ayu Putri Rahardja, Viryanando Evan Rizhar Eman Karunia Akbar Sin War Naw Viryanando Evan Rahardja Wibisono, Adi Ariyanto Wijaya, I Made Saka Yesha Ainensis El G. Pelupessy Yesha Ainesis El Gracianita Pelupessy