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All Journal IPTEKMA Agrotekno METAMORFOSA Journal of Biological Sciences Buletin Veteriner Udayana JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Itepa : Jurnal Ilmu dan Teknologi Pangan Jurnal Veteriner E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Media Ilmiah Teknologi Pangan (Scientific Journal of Food Technology) agriTECH Jurnal Ilmiah Teknologi Pertanian Agrotechno Indonesian Journal of Chemistry Agroindustrial Journal Seminar Ilmiah Nasional Teknologi, Sains, dan Sosial Humaniora (SINTESA)

NYOMAN SEMADI ANTARA

Program Studi Teknologi Industri Pertanian, Fakultas Teknologi Pertanian, Universitas Udayana, Bukit Jimbaran-Bali.

Author-ID : 2929399

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Education Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Public Health Social Sciences Veterinary

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Isolasi dan Karakterisasi Jamur Pelapuk Putih Pendegradasi Lignin dari Limbah Cair Pulp dan Kayu Lapuk Eukaliptus (Eucalyptus sp) Ida R Hasibuan; Nyoman Semadi Antara; I M. Mahaputra Wijaya
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 9 No 1 (2021): Maret
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

Lignin is an organic polymer compound that is difficult to degrade in the environment because of its very complex structure consisting of an aromatic ring group and three carbons in the side chain. This exploratory study aims to determine whether white rot fungi isolated from wastewater from eucalypt pulp and weathered wood are lignolytic and the ability of the isolates to degrade tannins as an approach in the lignin degradation process. The experimental design is divided into two stages, namely: 1) Isolation of fungi that have the ability to degrade tannins quantitatively and qualitatively as well as characterization of fungi morphologically (macroscopic and microscopic). 2) Testing the ability of superior white rot fungus isolates in degrading tannins at concentrations of 0.5%, 1%, 1.5% and 2% of tannins. The brown zone formed in white rot fungal isolates was measured the zone diameter and intensity of the brown color. The results of this study indicate that of the 29 isolates obtained, there were five superior isolates capable of degrading tannins, namely isolates LD06, LD07, BE01.3, BE01.4 and BE02.2. BE01.3 was isolated at 2% tannin concentration, the second largest diameter of the brown zone after LD06 isolate and the highest brown color intensity level three with a slightly dark brown color intensity, namely slightly blackish brown. Keywords: Isolation, characterization, white rot fungi, tannins, lignin degradation

ISOLASI DAN KARAKTERISASI BAKTERI POTENSIAL PENGHASIL ETANOL DARI INDUSTRI ARAK BALI DI KARANGASEM-BALI Yeni Veronika Simatupang; I Made Mahaputra Wijaya; Nyoman Semadi Antara
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 7 No 1 (2019): Maret
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

The aim of this reasearch was to isolate and identify ethanol-producing bacteria from the Balinese arak industry. Samples were taken from three places in Bali's Karangasem Regency. Isolation was conducted by growing microorganisms from samples on Zymomonas Sukrosa Mobilis (ZSM) media to obtain pure isolates. The pure isolates were then screened using selective media to obtain pure bacteria isolates. The isolates were then screened for ethanol producing bacteria. The ethanol determination were carried out qualitatively and quantitatively using potassium dichromate and scanning readings using UV-visible spectrophotometry. In the UV-visible spectra, the highest peak is observed at 579 nm. Seven best isolates were then grown in a 900 mL ZSM media and fermented for 10 days. The fermentation results were then distilled using a multilevel distillator. The best distillation alkohol result is 5.04±0,71 mL of ethanol produced by isolates with the code of BE-2410. Isolate BE-2410 were obtained from coconut fibers during fermentation. From the identification results, isolates BE-2410 was Grami-negative bacteria, rod bacteria, have the ability to produce catalase enzymes, and non-motile bacteria. This study proved that the bacteria had the ability to produce ethanol. Keywords : Arak, ethanol, fermentation, bacteria, bacteria isolation and identification, UV visible spectroscopy

PENGARUH LAMA PERENDAMAN DAN PEREBUSAN TERHADAP RESIDU INSEKTISIDA BERBAHAN AKTIF KLORPIRIFOS DAN NILAI NUTRISI KACANG PANJANG (Vigna sinensis) R. Ag. Ay. Ag. Bayu Chandraliawathy; I. G. A. Lani Triani; Nyoman Semadi Antara
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 4 No 3 (2016): September
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (363.671 KB)

The aims of the research were to determine the effect of soaking and boiling time on the chlorpyrifos residu level and the nutrition value, especially Vitamin C and crude fiber of long bean (Vigna sinensis). The other objective of the research was to find out the right soaking and boiling time to reduce chlorpyrifos residu and produce the good nutrition of long bean. The randomized block design was used in the experiment which the factorial pattern of the experiment was done with two factors, namely soaking time and boiling time. The result showed that the interaction treatment of soaking and boiling time influenced the chlorpyrifos residu and the nutrition value of long bean. The soaking time for 10 minutes and boiling time for 5 minutes could decrease the level of chlorpyrifos to 0,0427 ppm from 0,2364 ppm of fresh long bean. The vitamin C and crude fiber content of the long bean were 41,72 mg/g and 1,55% respectively. The color, texture, and the taste of the long bean were dark green, soft, and the panelist were still like the product. Keywords: chlorpyrifos residu, long beans, soaking, boiling, vitamin C, and crude fiber.

Pengaruh Konsentrasi Substrat Tepung Limbah Brem dan Lama Fermentasi Terhadap Produksi Kalsium Sitrat dengan Menggunakan Aspergillus niger ATCC 16404. Gusti Putu Agus Darmataba; Nyoman Semadi Antara; G.P. Ganda Putra
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 5 No 1 (2017): Maret
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

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Dregs brem is a waste of manufacture brem, from basic materials are made to be brem, only about 75% has managed to become brem while the remaining 25% to waste, brem waste still contains 10% sugar and starch by 12%. This research was done to determine the effects of substrate concentration and fermentation time on the production of calcium citrate from waste brem by using Aspergillus niger. The research was experimental research, using a randomized block design factorial design, consisting of two factors. The first factor is the substrate concentration (25%, 30% and 35%), the second factor was the fermentation time (3, 5, and 7 days). The results showed that the concentrations of substrate and fermentation time very significant effect on the level of calcium citrate, total soluble solids and acidity (pH). The interaction of both treatment effect was not significant on the level of calcium citrate, total soluble solids and acidity (pH) fermented flour waste brem using Aspergillus niger. The average value is highest Ca-citrate in the treatment of substrate concentration of 30% amounting to 4.38 g/L and fermentation time 5 days amounted to 4.76 g/L. [Keywords]: Aspergillus niger, flour waste Brem, substrate concentration, time of fermentation, calcium citrate

ISOLASI BAKTERI SELULOLITIK PENDEGRADASI SELULOSA DARI KOMPOS Zainul Arifin; Ida Bagus Wayan Gunam; Nyoman Semadi Antara; Yohanes Setiyo
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 7 No 1 (2019): Maret
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

This study aimed to isolate cellulolytic bacteria that have the potential to degrade cellulose taken compost samples from Temesi and Bondowoso, this study was also conducted to determine the ability of bacteria to degrade cellulose based on Congo red test and filter paper degradation test. All isolates were tested for cellulolytic activity using soluble Carboxymethyl Cellulose (CMC). Characterization was carried out by growing selected pure isolates on CMC media and then dripping 0.1% congo red to test the cellulolytic potential (cellulolytic potential was characterized by the emergence of clear zones around the colony). The results of isolation of bacteria obtained 38 isolates, namely 26 Bondowoso sample isolates and 12 Temesi isolates that were able to grow and utilize cellulose as a carbon source. But only fourteen isolates produced clear zones in the Congo red test with diameters ranging from 1.66 cm to 6.76 cm. Six isolates that have the largest diameters clear zone, were tested for degradation of filter paper (Whatman no. 1). Isolates bacteria of B2S8 obtained from Bondowoso compost samples has the highest ability to degrade cellulose on Whatman paper no. 1 as much as 51.30%. Keywords: Compost, Isolation, Screening, Cellulolytic Bacteria.

Skrining Isolat Bakteri Asam Laktat Penghasil Bakteriosin yang Diisolasi dari Asinan Rebung Bambu Tabah dan Ketahanannya terhadap Panas I Gede Arya Sujana; Nyoman Semadi Antara; Ida Bagus Wayan Gunam
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 8 No 4 (2020): Desember
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

The aim of this study to determine the Lactic Acid Bacteria (LAB) isolated from pickled tabah bamboo shoots can produce bacteriocins and the resistance to heat. This research was conducted in two stages, namely: 1) Screening of LAB isolates producing bacteriocin and 2) Testing the effect of heating temperatures of 80°C, 100°C and 121°C for 5, 10 and 15 minutes and without heating to bacteriocin resistance activity. In this study, the screening stage was carried out in two stages, namely: 1) antibacterial test with cell free supernatant and 2) antibacterial test with neutralized cell free supernatant. The resulting clear zone is observed then followed by calculating the value of Arbitrary Units (AU). The bacteria used as an indicator is Lactobacillus plantarum which is Gram positive bacteria. The results of this study indicated that of 88 LAB isolates there were 27 isolates that were able to produce bacteriocin. From 27 isolates, there were three isolates with the most potential to produce bacteriocins, namely isolate PR.6.10.5 with an inhibition zone of 17.67 mm and a bacteriocin activity value of 264.81 mm2/ml, isolate PR.6.15.2 with an inhibition zone of 17.33 mm and the bacteriocin activity value of 259.57 mm2/ml and isolate PR.3.15.1 with an inhibition zone of 17.00 mm and a bacteriocin activity value of 254.34 mm2/ml. Bacteriocins from LAB isolates isolated from pickled tabah bamboo shoots were able to withstand heating to a temperature of 121°C for 15 minutes. Bacteriocin activity decreased by an average of 27.75% after heating at 80°C, 100°C and 121°C for 5,10 and 15 minutes with a bacteriocin activity value from 141.82 mm2/ml to 254.34 mm2/ml. The smallest decrease in activity value occurred in PR.6.10.5 isolates with an average decrease of 22.98%. Keywords: Screening, lactic acid bacteria (LAB), bacteriocin, heat.

INHIBITION ACTIVITY of ESSENTIAL OIL of LEMONGRASS LEAVES (Cymbopogon citratus) ON THE GROWTH OF Escherichia coli, Staphylococcus aureus, AND Vibrio cholerae. Dwi Ayu Kirani Paramita; Nyoman Semadi Antara; Ida Bagus Wayan Gunam
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 2 No 1 (2014): Maret
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (492.412 KB)

The research aim was to determine the inhibition activity of essential oil of lemongrass (Cymbopogon citratus) on the growth of pathogenic bacteria. This research used a Completely Randomized Design with 5 treatments lemongrass oil concentration in 1% tween 80 namely 1%, 2%, 3%, 4%, 5% (v/v), and each concentration was tested to the three bacteria (Escherichia coli, Staphylococcus aureus, and Vibrio cholerae). Each treatment was repeated 3 times and the data were analyzed by analysis of variance, and followed by Duncan's Multiple Range Test. The results showed that lemongrass oil in tween 80 affected significantly the growth of Escherichia coli, Staphylococcus aureus and Vibrio cholerae. Inhibition against Escherichia coli, Staphylococcus aureus, and Vibrio cholerae at a concentration of 1% produces the highest activity with the inhibition diameter of 6,03 mm, 4,97 mm, and 9,67 mm, respectively. Vibrio cholerae was more sensitive againts lemongrass oil than Escherichia coli and Staphylococcus aureus.

Inhibition Activity of Basil Leaf Extract on The Growth of Eschericia coli, Salmonela typhi and Listeria monocytogenes Ida Ayu Ary Widnyani; Nyoman Semadi Antara; Ni Made Wartini
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 2 No 2 (2014): September
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

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The research aim was to determine the inhibition activity of basil leaf extract against Eschericia coli, Salmonela typhi, and Listeria monocytogenes, and to find out the minimum inhibitory concentration (MIC) of the extract. This research used split plot design consisting with 2 factors. First factor as a mai n plot was various solvents (Et-OH, Et-ast, and hexane) and the second factor as a sub main plot was various extract concentrations (100 ppm, 200 ppm, 300 ppm, 400 ppm, and 500 ppm). Each experiment was repeated 3 times and the data were analyzed by analysis of variances, followed by Duncan’s multiple range test. The result showed that the interaction of main plot and sub plot affected significantly, the growth of E. coli, S. typhi and L. monocytogenes. The extraction process using ethanol 70% produced the best extract of basil leaf. This extract could inhibit the growth of E. coli, S. typhi and L. monocytogenes with the MIC of about 200 ppm, 100 ppm and 200 ppm respectively. The extraction process using hexane produced basil leaf extract which it only could inhibit S. typhi with MIC of about 500 ppm. Otherwise, the extraction process using Et-ast produced basil leaf extract which it could inhibit S. typhi and L. monocytogenes with the MIC of about 200 ppm for both bacteria.

Potensi Ekstrak Cincau Hijau (Premna oblongifolia Merr.) dalam Menstimulasi Pertumbuhan Bakteri Asam Laktat Saluran Pencernaan Friska Ekagia Ginting; Nyoman Semadi Antara; I Made Mahaputra Wijaya
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 8 No 3 (2020): September
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

The green grass (Premna oblongifolia Merr), is a woody shrub plant mainly found in the forest. The main content of the plant is pectin, so green grass jelly is considered good source of dietary fiber, because the fiber can be fermented properly by digestive microflora (Gallaher, 2000). The fiber contained in green grass extract is believed to stimulate the growth of lactic acid bacteria in the digestive tract. The purpose of this study was to determine the potential of green grass jelly extract in stimulating the growth of digestive bacteria. The research was carried out in a laboratory with the treatment of adding concentrations of green grass jelly extract powder, and glucose as a control. The media used was Yeast-Pepton which added 1% glucose, green cincau extract powder 0.1%, 0.2%, 0.3%, 0.4%, 0.5%. Fermentation was carried out at 37 ° C with 18 hours fermentation time. The results showed that the concentration of the green grass extract 0.2% gave the best total lactic acid bacteria, this could because at this concentration the media had not increased the viscosity which inhibited bacterial growth. Keywords : green gras, pdigestive lactic acid bacteria, ectin.

STUDI PENGARUH pH AWAL MEDIA DAN LAMA FERMENTASI PADA PROSES PRODUKSI ETANOL DARI HIDROLISAT TEPUNG BIJI NANGKA DENGAN MENGGUNAKAN Saccharomycess cerevisiae Umi Fadilah; I Made Mahaputra Wijaya; N. Semadi Antara
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 6 No 2 (2018): April
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

Jackfruit seed (Artocarpus heterophyllus) flour is a substrate used in ethanol production research.The purpose of this study was to determine the effect of the media initial pH and the time length of fermentation on the ethanol fermentation process from the jackfruit seed starch hydrolyzate using Saccharomyces cerevisiae and to obtain optimum initial pH of the medium and the optimum fermentation length in order to obtain the highest ethanol concentration. This research was designed with 2 factors. The first factor is the initial pH of the media consisting of 3 levels, namely 4, 4,5, and 5. The second factor is the fermentation length consisting of three levels, ie 5, 6, and 7 days. Data obtained from the research are analyzed and presented descriptively. The results showed that the initial pH of the media had an effect on ethanol content and final pH, but had no effect on total soluble solids. Hydrolyzate of jackfruit flour, fermentation length has an effect on total ethanol, final pH, and total dissolved solids. The interaction of the two treatments had an effect on total ethanol of fermented jackfruit seed flour. The best treatment to produce ethanol hydrolyzate of jackfruit seed flour is the initial pH of medium 4.5 and the duration of fermentation of 6 days to produce total ethanol of 3.67 mL. Key words: Artocarpus heterophyllus, ethanol, fermentation, initial pH of medium

Co-Authors A.A.S. A. Sukmaningsih Ahmad Ali Akbar Ustadi Sadali Amna Hartiati Anak Agung Made Dewi Anggreni Anak Agung Putu Agung Suryawan Wiranatha Arfira Khofifah Awang Bagaskara Ayu Putu Sarasdewi Bambang Admadi Harsojuwono Dwi Ariesta Wijaya Putra Dwi Ayu Kirani Paramita Dwi Putri Nurmalasari Dylla Hanggaeni Dyah Puspaningrum Ella Dewi Yusnia Febriyawati Cahyanty Nugraha Febriyawati Cahyanty Nugraha Fitra Ayu Sitanggang Friska Ekagia Ginting G.P Ganda Putra G.P. Ganda Putra Gede Asstaradi Kusuma Gusti Putu Agus Darmataba Hendro Sukoco I Dewa Gde Mayun Permana I Dewa Made Sukrama I Gede Arya Sujana I Gusti Agung Teguh Gardipa I Gusti Ayu Ekawati I Gusti Ayu Lani Triani I Kadek Aditya Wirajaya I Ketut Satriawan I KETUT SUMIARTHA I Ketut Suter I Ketut Suter I M. Mahaputra Wijaya I Made Arif Sukmawan I Made Mahaputra Wijaya I Made Sukewijaya I Made Yoga Saputra I Nengah Kencana Putra I Nengah Widedianto I Nyoman Dibia I P. G. Urip Sanjaya Trisna I Putu Hendra Prasetya I W.G. SEDANA YOGA I Wayan Adi Wagestu I Wayan Arnata I WAYAN HENDRA KUSUMAYADI KUSUMAYADI I Wayan Suardana I Wayan Weta I Wayan Widia I. M. Mahaputra Wijaya Ida Ayu Ary Widnyani Ida Ayu Ketut Ariningsih Ida Bagus Djaya Utama Dauh Ida Bagus Wayan Gunam Ida R Hasibuan Irawaty Yolanda Hutajulu Kadek Dedi Widnyana Dinata Ketut Agus Ary Subakti Ketut Ratnayani KOMANG AYU NOCIANITRI Luh Gede Mening Lestari Luh Putu Wrasiati Lutfi Suhendra MARIA ULFA ELLA ELLA Muhammad Najri Mulyani Sri Ni Kadek Maya Isadora Ni Kadek Wiji Astuti Ni Made Ita Seri Utami Ni Made Wartini Ni Nyoman Puspawati Ni Putu Suwariani NI WAYAN SUNITI Nurul Lita Ajizah Nurul Octavia Wasis Pande Ketut Diah Kencana Purnama Yanti, Gusti Ayu Kade Diana Putri Cininta Giskha Putu Agus Nadiarta Putu Agus Nadiarta Putu Sudiarta R. Ag. Ay. Ag. Bayu Chandraliawathy Randall Howbert Rani Situmorang Rendy Sinaga Rian Lamhot Parasian Hutabarat Richard Howard Patty Rike Pratiwi Risky Simarmata Sarah Yohana Sihombing Sayi Hatiningsih Tuti Herlina Br.Naibaho Umi Fadilah Vinnod Gema Prabanca Wayan Redi Aryanta Yan Ramona Yeni Veronika Simatupang Yohanes Setiyo Zainul Arifin Zulkifly Al Haq

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