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PENGARUH PERLAKUAN DELIGNIFIKASI DENGAN LARUTAN NAOH DAN KONSENTRASI SUBSTRAT JERAMI PADI TERHADAP PRODUKSI ENZIM SELULASE DARI ASPERGILLUS NIGER NRRL A-II, 264 IDA BAGUS WAYAN GUNAM; KETUT BUDA; I MADE YOGA SEMARA GUNA
Jurnal Biologi Udayana Vol 14 No 2 (2010): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

his research was done in order to utilize agricultural waste (rice straw) as substrate to produce crude cellulase enzyme from Aspergillus niger. This research was conducted in two stages; the first stage was determination of the initial pH and fermentation time by pH treatment (5, 6 and 7) and fermentation time (7, 9 and 11 days). The second stage was determination of concentration of NaOH treatment and concentration of substrate, namely: concentrations of NaOH (2, 4 and 6%) and concentrations of substrate (1, 2 and 3% (w/v)). The results showed that the fermentation time of 9 days with the initial pH 6.0 was the optimal condition for production of crude cellulase enzyme from A. niger with rice straw as a substrate. The highest enzyme activity derived from interaction of delignification treatment with NaOH concentration of 6% and 2% rice straw substrate which produces endoglukanase enzyme activity (0.037 units/ml), filter paperase activity (0.033 units/ml), soluble protein (0.362 mg/ml), and specific activity of filter paperase (0.123 units/mg).
PENGARUH JENIS DAN JUMLAH PENAMBAHAN GULA PADA KARAKTERISTIK WINE SALAK Wayan Gunam, Ida Bagus; Wrasiati, Luh Putu; Setioko, Wito
Agrotekno Vol. 15, No. 1 Februari 2009
Publisher : Universitas Udayana

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Abstract

The research was aimed to determine the effect of typeand concentration of sugar on the characteristics of producedsalacca wine and to determine the type and concentration ofsugar yielding salacca wines with the best characteristics andpreferred by panelists. Sensory test results showed that themost preferred salaca wine characteristics by panelists wasthose which produced with addition of 25% sucrose sugar(w/v), and the produced wine contained 14.81% of ethanol,2.66% of total sugar, 0.21% of total acid, pH 3.67, 10.5°Brixof total soluble solids, and methanol content was notdetected.
PEMANFAATAN SAMPAH ORGANIK MENJADI PUPUK KOMPOS DENGAN BANTUAN MIKROORGANISME DI DESA SIBETAN KARANGASEM WAYAN GUNAM; I.B., L.P. WRASIATI; I MD. MAHAPUTRA WIJAYA; IW. ARNATA; I W.G. SEDANA YOGA
Buletin Udayana Mengabdi Vol 7 No 2 (2008): Volume 7 No.2 – September 2008
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Abstract

ABSTRACT Sibetan village which located in Karangasem Regency eastern of Bali is well known for its zallaca farming, that produces high quality and original taste of zallaca fruits. Zallaca,s post harvest process in farmer’s and distributor’s level produces enough quantity of organic waste. This waste currently is become a pollutant to their neighborhood and their soil. Even tough the majority of the waste is organic, it still became a disturbance because of its large volume. Hence, a special method or treatment is needed to solve this problem. Composting this organic waste through the helps of micro-organisms is became the selected method because this process is able to decreases the volume of the organic waste, and also the fermented waste is useful as an organic fertilizer. The fermented waste add a value to the zallaca’s farmer in order to decrease the dependency to artificial fertilizer, and helps increase the income of the zallaca’s farmer with the decrease allocations of farming expenses to bought an artificial fertilizer.
DELIGNIFIKASI AMPAS TEBU DENGAN LARUTAN NATRIUM HIDROKSIDA SEBELUM PROSES SAKARAIFIKASI SECARA ENZIMATIS MENGGUNAKAN ENZIM SELULASE KASAR DARI ASPERGILLUS NIGER FNU 6018 Gunam, Ida Bagus Wayan; Wartini, Ni Made; Dewi Anggreni, Anak Agung Made; Suparyana, Pande Made
Teknologi Indonesia Vol 34 (2011)
Publisher : LIPI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (16.048 KB) | DOI: 10.14203/jti.v34iKhusus.36

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Cellulose, the most abundant renewable resource, has received much attention as potential energy and carbon source for the production of useful products such as glucose, ethanol and fuels. The possibility of converting cellulose in bagasse enzymatically into glucose, after being loosened its complex structure chemically into primary one by using sodium hydroxide was studied. Bagasse was soaked in 6% sodium hydroxide for 12 hours at room temperature. This treatment resulted in loosening some cellulose bundle structure shown by release of lignin and hemicelluloses up to 32.11 and 42.87%, respectively and high water retention value of 15.90 (w/w). In this condition the delignifi ed bagasse could be saccharifi ed by crude cellulase enzym from Aspergillus niger. Saccharifi cation enzimatically of 2 g delignifi ed bagasse at 50oC pH 4,8 during 120 hours produced reducing sugar of 54.47 mg/100 ml.
PRODUKSI SELULASE KASAR DARI KAPANG TRICHODERMA VIRIDE DENGAN PERLAKUAN KONSENTRASI SUBSTRAT AMPAS TEBU DAN LAMA FERMENTASI IDA BAGUS WAYAN GUNAM; WAYAN REDI ARYANTA; IDA BAGUS N. SURYA DARMA
Jurnal Biologi Udayana Vol 15 No 2 (2011): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

This research was done in order to utilize bagasse as substrates to produce crude cellulase from Trichoderma viride. This research used a randomized block design with factorial pattern which consisted of two factors. The first factor was the concentration of substrate which consisted of three levels namely, substrate concentration of 1%, 2%, and 3%. The second factor was the fermentation time which consisted of three levels namely, 5, 7, and 9 days. Each treatment classified into two groups based on time of production. The results showed that the concentration of bagasse and fermentation time significantly influenced the parameters observation of crude cellulase production from Trichoderma viride. The optimal treatment combination to produce crude cellulase with maximum activity was the treatment of 3% substrate concentration and fermentation time of 7 days with an average value of cellulase activity (filter paperase), soluble protein, and cellulase specific activity were 0.771 Unit/mL, 0.262 mg/mL, and 2.940 Unit/mg, respectively.
Karakteristik Sawi Hijau (Brassica rapa var parachinensis) yang Dihasilkan dari Aplikasi Bakteri Pemacu Pertumbuhan I Gusti Ayu Lani Triani; Ida Bagus Wayan Gunam
Jurnal Ilmiah Teknologi Pertanian Agrotechno Vol 7 No 1 (2022)
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JITPA.2022.v07.i01.p08

Abstract

This study tries to use the roots of bamboo plants that grow in Bali as Plant Growth Promoting Rhizobacteria and applied to vegetable plants to see the physical characteristics of these vegetables. This study aims to determine the physical characteristics of greens mustard produced by using the application of growth-promoting bacteria derived from the roots of bamboo (Bambusa maculata). The characteristics of the greens mustard were compared with the use of commercial PGPR (products from Central Java farmers) and without the use of growth-promoting bacteria. Based on the results of the research on the physical characteristics of greens mustard (Brassica rapa var parachinensis) the results of the application of growth-promoting bacteria from bamboo roots obtained plant heights ranging from 39 cm, weight 34 – 44 g, number of leaves 9 – 12 pieces, texture 22.18 – 30.31 kg .m/sec2, brightness level 42.13 – 47.13 and total dissolved solids 4.4 – 5.1% brix, slightly different from the treatment without PGPR and commercial PGPR. For this reason, it is necessary to continue cultivation with growth-promoting bacteria derived from natural ingredients that grow in Bali, this is an effort towards environmentally friendly cultivation so that it is expected to get quality results and are safe for consumption.
Rebung Bambu Tabah (Gigantochloa Nigrociliata) Berpotensi Sebagai Bahan Afrodisiak pada Mencit Jantan (POTENTIAL APHRODISIAC ACTIVITY OF TABAH BAMBOO SHOOTS (GIGANTOCHLOA NIGROCILIATA) IN MALE MOUSE) Anak Agung Sagung Alit Sukmaningsih; Ida Bagus Wayan Gunam; Nyoman Semadi Antara; Pande Ketut Diah Kencana; I Wayan Widia
Jurnal Veteriner Vol 18 No 3 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (167.876 KB) | DOI: 10.19087/jveteriner.2017.18.3.393

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This study was conducted to observe the potential aphrodisiac activity of Gigantochloa nigrociliata in male mouse Mus musculus. The twenty four male mice, aged 12 weeks were divided randomly into four groups, each group consisted of 6 mice. One group was used as control (P0) where the mice were treated with aquadest. Three other groups were given treatment. The extracts contained of water (P1), etanol (P2), and n-hexane (P3). The animals in each treatment were treatments given 200 mg/kg bw Gigantochloa nigrociliata of extract orally (gavage) once daily as much as 0,2 mL for 33 days. The observations of sexual behaviour performed every three days during the treatment. Variables observed for the sexual behaviour are both the number and latency of mount and intromission. Mice were anaesthetized after 33 days. Cauda epidydimis were analyzed for motility, number and viability of sperms. The result showed that testosterone serum level increased significantly on etanol extract group (p<0.05). There were significantly increased the mounting number, intromission number with reduction in mounting latency, and intromission latency of male mice (P<0.05). There was significant an increase in the number of (a) sperm motility and number of sperm on etanol extract group (p<0.05). The results of the present study demonstrate that Gigantochloa nigrociliata extract improve sexual behaviour in male mouse. ABSTRAK Telah dilakukan penelitian mengenai potensi rebung bambu tabah sebagai bahan afrodisiak yang diujikan pada mencit jantan (Mus musculus). Sebanyak 24 mencit dikelompokkan menjadi empat perlakuan dan enam ulangan. Satu kelompok digunakan sebagai kontrol (P0). Tikus pada kelompok kontrol diberikan aquades. Tiga kelompok lainnya diberikan perlakuan ekstrak air bambu tabah (P1), ekstrak etanol bambu tabah (P2), dan ekstrak n-hexane bambu tabah (P3). Setiap hewan pada masing masing perlakuan diberikan ekstrak 200 mg/kg bb (bobot badan) sebanyak 0,2 mL ) secara oral selama 33 hari. Pengamatan perilakukawin berupa mount dan intromission dilakukan setiap 3 hari sekali. Setelah 33 hari hewan dikorbankan untuk dikoleksi epididimis bagian cauda yang digunakan dalam analisis spermatozoa dan pengambilan darah yang digunakan dalam analisis hormon testosteron serum. Hasil analisis dengan menggunakan uji one way Anova menunjukkan adanya perbedaan kadar hormon testosteron serum secara signifikan (p<0,05). Kelompok hewan dengan perlakuan etanol memiliki kadar hormon yang lebih tinggi dibandingkan kelompok lain. Kelompok hewan dengan perlakuan heksan memiliki kadar hormon terendah dengan perbedaan yang signifikan dibandingkan dengan kelompok perlakuan lain. Terjadi pula perbedaan motivasi seksual yang diamati pada perilaku kawin berupa mount dan intromission. Perbedaan jumlah dan waktu terjadinya mount dan intromission terjadi secara signifikan di antara kelompok perlakuan dan kontrol (p<0,05). Peningkatan jumlah mount dan intromission, serta semakin singkatnya waktu untuk mencapai mount dan intromission terjadi secara signifikan dibandingkan dengan kontrol dan diantara kelompok perlakuan (p<0,05). Hasil analisis data juga menunjukkan peningkatan motilitas spermatozoa tipe (a) yakni spermatozoa dengan gerakan yang progresif maju ke depan dan jumlah spermatozoa, terutama pada esktrak etanol rebung secara signifikan. Disimpulkan bahwa rebung bambu berpotensi sebagai bahan afrodisiak karena dapat meningkatkan hormon testosteron yang merupakan salah satu faktor penting dalam pengaturan sistem reproduksi serta dapat meningkatkan motivasi seksual yang diuji pada mencit jantan.
Biodesulfurization of the mixture of dibenzothiophene and its alkylated derivatives by Sphingomonas subarctica T7b Ida Bagus Wayan Gunam; Teruo Sone; Kozo Asano
Indonesian Journal of Biotechnology Vol 26, No 3 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.62584

Abstract

Organosulfur compounds classified as dibenzothiophenes (DBTs) and their derivatives are contained in petroleum. When used as fuel, these substances release SOx emissions, thus contributing to air pollution, acid rain, and climate change. Therefore, it is necessary to reduce the content of these organic sulfur compounds in fuels and one way to achieve this is through bacterial desulfurization. This study reports the biodesulfurization process of a mixture of DBT, 4-hexyl DBT, 4,6-dibutyl DBT, and various organosulfur compounds in light gas oil (LGO). The experiment was conducted by treating 1 mL of aromatic organosulfur compounds with 100 mg/L in \textit{n}-tetradecane or 1 mL LGO with 5 mL mineral salts in sulfur-free medium, incubated at 27 °C for 5 days with shaking at 273 rpm. Gas chromatography analyses revealed that the growing Sphingomonas subarctica T7b cells desulfurized and converted 88.29% of DBT to 2-hydroxybiphenyl as a metabolite while a mixture of DBT and 4,6-dibutyl DBT was desulfurized at 86.40\% and 7.00%, respectively. Furthermore, the mixture of DBT, 4-hexyl DBT, and 4,6-dibutyl DBT had a desulfurization percentage of 84.40%, 41.00%, and 6.66%, respectively, after five days of incubation. The compounds were observed to desulfurize slightly better as single compounds compared to when mixed with other aromatic sulfur compounds.
Pemanfaatan Tepung Rumput Laut Gracilaria sp. pada Tempe sebagai Alternatif Pangan Sumber Yodium Monikasari, Ni Nyoman Trisa; Wayan Gunam, Ida Bagus; Wisaniyasa, Ni Wayan
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 16, No 1 (2021): Juni 2021
Publisher : Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jpbkp.v16i1.689

Abstract

Tempe merupakan produk pangan bergizi tinggi yang digemari dan diterima oleh masyarakat luas, tetapi umumnya memiliki kadar yodium yang rendah. Rumput laut yang kandungan yodiumnya cukup tinggi dapat digunakan sebagai bahan fortifikasi yodium pada makanan. Penambahan rumput laut pada tempe diharapkan dapat menjadi alternatif pangan sumber yodium untuk menanggulangi masalah GAKY (Gangguan Akibat Kekurangan Yodium). Tujuan penelitian ini adalah untuk mengetahui pengaruh penambahan tepung rumput laut Gracilaria sp. terhadap kadar yodium pada tempe serta mengetahui karakteristik yang dihasilkan. Perlakuan yang dilakukan adalah perbandingan kedelai dan tepung rumput laut 100:0; 97,5:2,5; 95:5; 92,5:7,5; dan 90:10. Perbandingan kedelai dan tepung rumput laut 92,5:7,5 merupakan komposisi terbaik yang menghasilkan tempe dengan kadar yodium 0,26 mg/g; nilai sensoris hedonik rata-rata 5 (agak suka), serta 100 gram tempe tersebut memenuhi 17,47% angka kecukupan gizi (AKG) yodium orang dewasa per hari. Studi ini menunjukkan bahwa tempe tersebut dapat digolongkan sebagai pangan sumber yodium karena telah memenuhi 15% AKG orang dewasa.ABSTRACTTempeh is a highly nutritious food that is popular and accepted by diverse community groups, but has low iodine levels. Seaweed, with a high iodine content can be used as a fortification ingredient to increase the iodine levels of the foods. The addition of seaweed in tempeh is expected to be used as an alternative food source of iodine contents to overcome the IDD (Iodine Deficiency Disorders) problem. This study aimed to determine the effect of the addition of Gracilaria sp. seaweed flour to iodine levels in tempeh and to determine the characteristic of the product. The treatments were 5 (five) compositions of soybean and seaweed flour at ratio of (100:0), (97.5:2.5), (95:5), (92.5:7.5), and (90:10). The composition of soybean and seaweed flour at ratio of 92.5:7.5 was the best composition that produces tempeh with an iodine content of 0.26 mg/g; hedonic sensory test results on average 5 (somewhat like) and 100 g of tempeh met 17.47% of the daily iodine requirements for the adults. This study showed that tempeh can be considered as an alternative food source of iodine content as it contributed to 15% of adult’s daily nutritional adequacy.
Pengaruh Konsentrasi Starter dan Gula terhadap Karakteristik Wine Salak Ida Bagus Wayan Gunam
Jurnal Ilmiah Teknologi Pertanian Agrotechno Vol 3 No 1 (2018)
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JITPA.2018.v03.i01.p05

Abstract

Penelitian ini bertujuan untuk menentukan konsentrasi starter dan penambahan gula yang optimal untuk memperoleh karakteristik wine salak yang terbaik. Penelitian ini menggunakan Rancangan Acak Kelompok pola factorial dengan dua faktor. Faktor pertama adalah konsentrasi starter yaitu: 5, 10 dan 15% (v/v) dan faktor kedua adalah penambahan gula yaitu: 10, 15, 20 dan 25% (b/v). Hasil penelitian menunjukkan bahwa konsentrasi starter dan penambahan gula berpengaruh terhadap karakteristik wine salak. Pada konsentrasi gula yang lebih tinggi dapat menhasilkan kadar alkohol yang lebih tinggi juga, karena terdapat lebih banyak substrat yang dimanfaatkan oleh khamir untuk memproduksi alkohol. Wine salak dengan karakteristik terbaik diperoleh dari perlakuan konsentrasi starter 15% (v/v) dan penambahan gula 25% (b/v), dengan kadar alkohol tertinggi yaitu sebesar 12,25% dan juga memiliki nilai uji efektifitas terbaik yaitu sebesar 1,33.
Co-Authors A. A. Made Dewi Anggreni A.A.M. Dewi Anggreni A.A.Made Dewi Anggreni Ambar Kusumaningrum Amna Hartiati Anak Agung Made Dewi Anggreni Anak Agung Made Dewi Anggreni Azis Akbar Hakim Bimby Issassam Cokorda Anom Bayu Sadyasmara Deddy Setiadi Kadir Madjid Dwi Ayu Kirani Paramita Ella Dewi Yusnia Fitra Ayu Sitanggang G.P. Ganda Putra G.P. Ganda Putra Gde Agung Bagus Surya Adnyana Gede Bagus Suwarrizki G. P Hamonangan Sipayung I B. K. Widnyana Yoga I Dewa Putu Rega Elyana I G.A. Lani Triani I Gede Arya Sujana I Gede Krisna Putra Pratama I Gusti Ayu Lani Triani I Gusti Bagus Ananta Wijaya Putra I Gusti Ngurah Agung Paranatha I Kadek Adi Wijaya Putra I Komang Trikuti I M. Mahaputra Wijaya I Made Mahaputra Wijaya I Made Mahaputra Wijaya I Made Supartha Utama I Made Supartha Utama I Made Supartha Utama I Made Yoga Saputra I MADE YOGA SEMARA GUNA I Putu Hendra Prasetya I W. Wisma P. Putra I W.G. SEDANA YOGA I Wayan Arnata I Wayan Kasa I Wayan Suarta I Wayan Widia I Wayan Wisma Pradnyana Putra I.B., L.P. WRASIATI Ida Bagus Gede Brahmantara IDA BAGUS N. SURYA DARMA Ida Bagus Wisnu Matra Atmaja Janurianti , Ni Made Defy Janurianti, Ni Made Defy Ketut Agus Ary Subakti KETUT BUDA Kozo Asano Luh Putu Wrasiati Lutfi Suhendra Mahaputra Wijaya Michael Jordi Theofanny Monalisa Nababan Monikasari, Ni Nyoman Trisa Muhammad Iqbal N.P.I. Widyantari Nebay Cronika Simbolon Ni Kadek Eni Juniantari Ni Made Defy Janurianti Ni Made Defy Janurianti Ni Made Wartini Ni Made Wartini Ni Made Wartini Ni Made Wartini Ni Putu Puspadi Aristyanti Ni Putu Suwariani Ni Wayan Wisaniyasa Nursatria Purba Nurul Octavia Wasis NYOMAN SEMADI ANTARA Odilia Keron Pande Ketut Diah Kencana Pande Komang Suparyana Putu Agus Nadiarta Putu Setia Budi Rike Pratiwi Sayi Hatiningsih Shenni Maulina Teguh Pribadi Girsang Teruo Sone WAYAN REDI ARYANTA Wito Setioko Yohanes Setiyo Yohannes Eko Putra Simanullang Yudisthira Dharma Bhusana Dasa Zainul Arifin