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All Journal Agrotekno METAMORFOSA Journal of Biological Sciences Buletin Udayana Mengabdi JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Itepa : Jurnal Ilmu dan Teknologi Pangan Jurnal Veteriner Jurnal Biologi Udayana SIMBIOSIS Media Ilmiah Teknologi Pangan (Scientific Journal of Food Technology) Indonesian Journal of Biotechnology Jurnal Ilmiah Teknologi Pertanian Agrotechno Jurnal Teknologi & Industri Hasil Pertanian Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Teknologi Indonesia Canrea Journal: Food Technology, Nutritions, and Culinary Journal Jurnal Gastronomi Indonesia AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) SEAS (Sustainable Environment Agricultural Science) Seminar Ilmiah Nasional Teknologi, Sains, dan Sosial Humaniora (SINTESA)

Ida Bagus Wayan Gunam

Program Studi Teknologi Industri Pertanian, Fakultas Teknologi Pertanian, Universitas Udayana, Badung, Bali, Indonesia.

Author-ID : 2929439

Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Education Electrical & Electronics Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Social Sciences Veterinary

Published : 67 Documents Claim Missing Document

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ISOLASI BAKTERI POTENSIAL PENDEGRADASI DIBENZOTIOFENA DARI TANAH TERCEMAR MINYAK BUMI DI BULUH TELANG LANGKAT SUMATERA UTARA I Putu Hendra Prasetya; Ida Bagus Wayan Gunam; Nyoman Semadi Antara
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 4 No 1 (2016): Maret
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (457.051 KB)

This research aimed to isolate some of potential bacteria which could degrade dibenzothiophene (DBT). The bacteria were isolated from the soil which has been contaminated by petroleum for many years in Buluh Telang Langkat North Sumatera. This experiment was also conducted to find out the isolate that had the highest degradation rate of DBT. The results showed that the isolated bacteria had a different ability to degrade dibenzothiophene with OD660 ranged from 1.100 to 1.137 and the degradation rate ranged from 42.62% to 69.88%. The isolate of LSU20 had the highest ability to degrade DBT. The isolated bacteria which was incubated for 96 hours at 45 o C in shaking condition at 150 rpm could degrade 69.88% of 200 ppm DBT in tetradecane. Keywords: Isolation, biodesulfurization, dibenzothiophene

Uji Angka Lempeng Total dan Kontaminan Koliform pada Susu Kedelai Bermerek yang Beredar di Kota Denpasar Michael Jordi Theofanny; Ida Bagus Wayan Gunam; Ni Putu Suwariani
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 9 No 1 (2021): Maret
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

This research aimed to testing total plate count and coliform contaminant on legal product soy milk sold in Denpasar city is qualified to Indonesian National Standard (SNI) and safe to consumption. Soy milk has high nutrition and good for growth microorganisms. Microorganisms in soy milk is good for health but be worried have a dangerous microorganisms. Samples of soy milk based on purposive method, tested with total plate count agar with pour plate method and contaminant coliform with most probable number method. The result of testing total plate count, all samples is under maximum of SNI 5 × 104CFU/g. After that soy milk sample testing of coliform contaminant, the result is all samples has negative coliform contaminant. Conclusion of the research is all of the legal product soy milk sold in Denpasar qualified to SNI No. 01-3830-1995 and safe to consumption. Keyword: coliform, Denpasar city, Indonesian national standard, soy milk, total plate count.

OPTIMASI SUHU DAN pH TERHADAP AKTIVITAS ENZIM ENDOGLUKANASE MENGGUNAKAN RESPONSE SURFACE METHODOLOGY (RSM) Ambar Kusumaningrum; Ida Bagus Wayan Gunam; I Made Mahaputra Wijaya
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 7 No 2 (2019): Juni
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

This study aims to determine the optimal temperature and pH for the growth of cellulolytic bacterial isolates to produce high cellulase enzyme activity. This study used one potential cellulolytic bacterial isolate B2S8 which had the highest cellulose degradation value and highest cellulase enzyme activity in previous studies. Carboxymethyl Cellulose (CMC) was used as a substrate on growth media and enzyme activity test. Optimization of temperature and pH on cellulase enzyme activity was done by Response Surface Methodology (RSM) by Central Composite Design (CCD). The results using Response Surface Methodology (RSM) showed that the highest endoglucanase enzyme activity was at 36.9 ºC and pH 6.9 was equal to 0.0269 IU/mL. Keywords: cellulolytic bacteria, endoglucanase enzyme, activity, Response Surface Methodology.

PENGARUH PERSENTASE PENGAMBILAN VOLUME DISTILAT CAIRAN PULPA HASIL SAMPING FERMENTASI BIJI KAKAO TERHADAP KARAKTERISTIK CUKA FERMENTASI I Dewa Putu Rega Elyana; G.P. Ganda Putra; I.B. Wayan Gunam
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 5 No 3 (2017): September
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

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The by-product of fermented cocoa seeds could be fermented into cocoa vinegar. However, the product requires to be distilled to be purified. In this study, an experiment was conducted to determine the percentage in taking the best distillate to produce the best characteristics of cacao vinegar. The percentage was started from 30, 50, 70, and 90% (v/v). The best treatment was determined according to the total acids, acetic acid, total sugars, total soluble solids and pH and distillation time. The result showed that taking distillate volume affected the total acid, acetic acid, and distillation time, and did not affect the total soluble solids, total sugar and pH value. The best distillate preparation of distillate process of cacao vinegar was volume 90% (v/v) with total acid of 0,25 meq NaOH/g, 1,51% acetic acid, pH 2,96, total sugar 0,079%, total soluble solids 1,13oBrix, with a distillation time of 120,25 minutes.Keywords: cocoa, distillation, volume percentage, fermented vinegar

ISOLASI DAN SKRINING BAKTERI SELULOLITIK DARI BEBERAPA TANAH HUTAN DI BALI Ella Dewi Yusnia; Ida Bagus Wayan Gunam; Nyoman Semadi Antara
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 7 No 1 (2019): Maret
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

Soil is one habitats of the cellulolytic bacteria. Soil containing manure and decayed wood is habitation for cellulolytic bacteria. This research is aimed to isolate and screen cellulolytic bacteria from the soil in Bali that has the potentials to degrade cellulose. The soil samples ware taken from forest in Gunaksa-Klungkung, Telaga-Karangasem, Sukahat-Karangasem, Gilimanuk-Jembrana, and Mangrove forests in Suwung-Denpasar. Each single cellulolytic bacteria colonies which grown on solid media containing carboxymethyl cellulose (CMC) were isolated. Screening of cellulolytic bacteria using congo red 0,1 %, staining bacterial isolates which were created clear zones in around the colony were selected. The isolation result are 67 isolated cellulolytic bacteria covered in five soils sample. Twenty-one bacterial isolates were producing cellulase enzymes with an indication of a clear zone in around the colony. Nine superior isolates have high cellulolytic index (CI), namely: G2-8 (5.41), G1-4 (4.86), G2-10 (4.5), G2-2 (3.64), M1-5 (3.10), G2-5 (3.03), M2-12 (2.72), G1-1 (2.38), and M1-1 (2.21). The obtained highest percentage of filter paper (Whatman No.1) degradation was 8.32% and the lowest was 2.48%. Keywords: forest soil, isolation, cellulolytic bacteria, congo red

Pengaruh Penambahan Feri Klorida pada Media NPSi terhadap Biomassa dan Kandungan Protein Mikroalga Chaetoceros calcitrans I Gusti Bagus Ananta Wijaya Putra; Anak Agung Made Dewi Anggreni; Ida Bagus Wayan Gunam
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 5 No 1 (2017): Maret
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

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This study aims were to know the effect of ferric chloride (FeCl3) on biomass concentration andprotein content of microalgae Chaetoceros calcitrans and determine the best concentration of ferricchloride for production of microalgae Chaetoceros calcitrans with highest protein content. This studywas designed with randomized block design with single factor. The factor was different ferric chlorideconcentration consist of 4 concentration, i.e. 0 µM, 12 µM, 24 µM and 36 µM. Each treatment wasgrouped into 4 based on time of cultivation. The data obtained were analyzed by analyzed of variancefollowed by least significant difference test, if a treatment had significant effect. The result showed thataddition of ferric chloride had very significant effect on the b iomass concentration and protein content.The highest biomass concentration and protein content was obtained from the microalgae Chaetoceroscalcitrans which was cultivated in 36 µM ferric chloride amounted of 4.1 x 107cell/mL and 25.41%. Keywords: biomass, Chaetoceros calcitrans, Ferric Chloride, NPSi, protein.

STIMULASI PERTUMBUHAN Lactobacillus casei subsp. rhamnosus PADA MEDIA YANG DISUPLEMENTASI TEPUNG REBUNG BAMBU TABAH (Gigantochloa nigrociliata Buse-Kurz) Ketut Agus Ary Subakti; Nyoman Semadi Antara; Ida Bagus Wayan Gunam
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 4 No 1 (2016): Maret
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

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Shoots of tabah bamboo (Gigantochloa nigrociliata Buse-Kurz) are known containing dietary fiber and oligosaccharides as a prebiotic potential. The components contained in the bamboo shoots are expected to enhance the growth of the microflora of the digestive tract including lactic acid bacteria (LAB). This study was conducted to determine the ability of the bamboo shoot flour as a substrate to stimulate the growth of Lactobacillus casei subsp. rhamnosus. In vitro experiment was conducted in the laboratory by treatment of fermentation time. The media used was Tr-YP media, which was yeast-peptone media added by 2 g bamboo shoots powder per 100 ml. Fermentation was carried out at 37°C with a fermentation time of 0 hours to 24 hours. The results showed that during the fermentation the growth of Lactobacillus casei subsp. rhamnosus were stimulated by the media that supplemented by bamboo shoot powder. The growth of the bacteria were followed by the decrease in pH. Total Lactobacillus casei subsp. rhamnosus after 12 hours fermentation was 5,5x10 8 CFU/ml and the pH was 5,13. [Keywords]: Gigantochloa nigrociliata, Lactobacillus casei subsp. rhamnosus, flour bamboo shoots

PENGARUH SUHU DAN KONSENTRASI ENZIM AMILOGLUKOSIDASEPADA PROSES SAKARIFIKASI TERHADAP PRODUKSI GULA CAIR PATI UBI TALAS (Colocasia esculenta) I Kadek Adi Wijaya Putra; Amna Hartiati; Ida Bagus Wayan Gunam
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 3 No 2 (2015): Juni
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

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The experiment was carried out todetermine the effect of amyloglucosidase concentration and temperature of saccharification process on the characteristics ofliquid sugar and to find out the best characteristics of liquid sugar from taro potato starch. This study used a split plot experimental design with two treatment factors. The main plot was the saccharification temperature which consists of three levels, namely 55°C, 60°C and 65°C. Amyloglucosidase enzyme concentration as the subplot consisted of three levels ie 1500 U/kg of starch, 2500 U/kg of starch and 3500 U/kg of starch. In this experiment obtained 9 combinations for each experiment was repeated 2 times and obtained 18 experimental units. The results of liquid sugar were then analyzedtheir moisture content, ash content, degree of sweetness, and sensory test color. The results showed that the addition of enzyme amyloglucosidase with different concentrations significantly affected the degree of sweetness and gave a very significant effect on the color of liquid sugar and no significant effect on moisture content and ash content. The difference treatment of Saccharification temperature significantly affected the water content, the degree of sweetness and color and no gave significant effect on ash content of liquid sugar. Interaction between treatments significantly affected the degree of sweetness, very significant effect on the color and no significant effect on moisture content and ash content of the resulting liquid sugar. The treatment with the addition of enzyme amyloglucosidase with concentration of 3500 U/kg starch and temperature of saccharification 55°C producedthe best characteristics of liquid sugars from starch of taro potato.

ISOLASI DAN KARAKTERISASI KHAMIR POTENSIAL PENGHASIL BIOETANOL DARI INDUSTRI ARAK DI KARANGASEM BALI Nebay Cronika Simbolon; I Made Mahaputra Wijaya; Ida Bagus Wayan Gunam
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 6 No 4 (2018): Desember
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

This research aimed to isolate and identify potential yeast of bioethanol-producer from arak industri in Karangasem Bali. The isolated sample was taken from 3 different points from 2 villages in Karangasem Bali. Isolation was carried out using PYG media then purified to obtain pure isolates. The pure isolates were screened with several stages, namely a qualitative test of gas production, growth selection with the addition of antibiotics, and quantitative tests with an alcohol oxidation reaction. Determind by UV visible spectroscopy, 9 potential isolates was obtained to continue to the identification stage. At this stage fermentation was carried out in PYG media for 10 days using a starter from a potential isolate with a media glucose level of 20% of the media volume. Fementation results were then distilled. Of the obtained 9 potential isolates the best isolate. IS 258 isolates are determind the best isolates with 86.85 mL ethanol. The total ethanol produced by IS 258 is higher than with 60.73 mL alcohol control experiment (Alcotec). Isolate IS 258 was isolated from bayur skin samples taken from the sap fermentation process. IS 258 then identified macroscopically and microscopically. Based on the results of macroscopic and microscopic identification, IS 258 has many similarities with previous studies on yeast isolation, isolate IS 258 is presumably the yeast genus Saccharomyces sp. Further research is needed to optimize ethanol production of IS 258 and identify species from isolates IS 258. Keyword : bioethanol, Balinese wine, lau, yeast, isolation and identification of yeast, UV visible spectroscopy

Uji Potensi Bakteri Selulotik dari Lahan Pertanian yang Tercemar Pestisida Putu Setia Budi; Ida Bagus Wayan Gunam; Anak Agung Made Dewi Anggreni
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 4 No 1 (2016): Maret
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (589.002 KB)

The aim of this study was to isolate bacteria that could potentially degrade the cellulose from of agricultural land in the village Candikuning Bedugul and to determine the ability of isolates in degrade cellulose.These bacteria are capable of utilizing cellulose as carbon source 11 only isolates that produce a clear zone with diameters above 12.07 mm up to 45.59 mm on Gram ’sIodine test. Eleven isolates bacteria cellulolytic pure who afford occurrence bacterial growth on media selective and isolates produce clear zone that most highest is CK 18.Keywords: cellulose degrading bacteria, isolation, Gram's Iodine test.

Co-Authors A.A.S. A. Sukmaningsih Ambar Kusumaningrum Amna Hartiati Anak Agung Made Dewi Anggreni Azis Akbar Hakim Bambang Admadi Harsojuwono Bimby Issassam Deddy Setiadi Kadir Madjid Dwi Ayu Kirani Paramita Dylla Hanggaeni Dyah Puspaningrum Ella Dewi Yusnia ENIEK KRISWIYANTI Fitra Ayu Sitanggang G.P. Ganda Putra Gde Agung Bagus Surya Adnyana Gede Bagus Suwarrizki G. P Hamonangan Sipayung I B. K. Widnyana Yoga I Dewa Gde Mayun Permana I Dewa Putu Rega Elyana I Gede Arya Sujana I Gede Krisna Putra Pratama I Gusti Ayu Lani Triani I Gusti Bagus Ananta Wijaya Putra I Gusti Ngurah Agung Paranatha I Kadek Adi Wijaya Putra I Komang Trikuti I M. Mahaputra Wijaya I Made Mahaputra Wijaya I Made Mahaputra Wijaya I Made Supartha Utama I MADE SUPARTHA UTAMA I Made Yoga Saputra I MADE YOGA SEMARA GUNA I Putu Hendra Prasetya I W W Aryanika I W. Wisma P. Putra I W.G. SEDANA YOGA I Wayan Arnata I Wayan Kasa I Wayan Suarta I Wayan Widia I Wayan Wisma Pradnyana Putra I.M.S. Wijaya IDA AYU ASTARINI Ida Bagus Gede Brahmantara IDA BAGUS N. SURYA DARMA Ida Bagus Wisnu Matra Atmaja Janurianti , Ni Made Defy Janurianti, Ni Made Defy Ketut Agus Ary Subakti KETUT BUDA Kozo Asano Luh Putu Wrasiati Lutfi Suhendra Made Ria Defiani Mahaputra Wijaya Michael Jordi Theofanny Monalisa Nababan Monikasari, Ni Nyoman Trisa Muhammad Iqbal N.P.I. Widyantari Nebay Cronika Simbolon Ni Kadek Eni Juniantari Ni Made Defy Janurianti Ni Made Ratih Despianti Ni Made Wartini Ni Putu Puspadi Aristyanti Ni Putu Suwariani Ni Wayan Wisaniyasa Noval Wahyu Adi Nursatria Purba Nurul Octavia Wasis NYOMAN SEMADI ANTARA Odilia Keron Pande Ketut Diah Kencana Pande Komang Suparyana Putu Agus Nadiarta Putu Setia Budi Rendy Sinaga Rike Pratiwi Sadyasmara, Cokorda Anom Bayu Sayi Hatiningsih Shenni Maulina Teguh Pribadi Girsang Teruo Sone Thobie E. B Kaban WAYAN REDI ARYANTA Wito Setioko Yohanes Setiyo Yohannes Eko Putra Simanullang Yudisthira Dharma Bhusana Dasa Zainul Arifin

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