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All Journal JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI
Zainul Arifin
Mahasiswa Program Studi Teknologi Industri Pertanian, Fakultas Teknologi Pertanian, Unud
Agriculture, Biological Sciences & Forestry

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ISOLASI BAKTERI SELULOLITIK PENDEGRADASI SELULOSA DARI KOMPOS Zainul Arifin; Ida Bagus Wayan Gunam; Nyoman Semadi Antara; Yohanes Setiyo
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 7 No 1 (2019): Maret
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (384.898 KB) | DOI: 10.24843/JRMA.2019.v07.i01.p04

Abstract

This study aimed to isolate cellulolytic bacteria that have the potential to degrade cellulose taken compost samples from Temesi and Bondowoso, this study was also conducted to determine the ability of bacteria to degrade cellulose based on Congo red test and filter paper degradation test. All isolates were tested for cellulolytic activity using soluble Carboxymethyl Cellulose (CMC). Characterization was carried out by growing selected pure isolates on CMC media and then dripping 0.1% congo red to test the cellulolytic potential (cellulolytic potential was characterized by the emergence of clear zones around the colony). The results of isolation of bacteria obtained 38 isolates, namely 26 Bondowoso sample isolates and 12 Temesi isolates that were able to grow and utilize cellulose as a carbon source. But only fourteen isolates produced clear zones in the Congo red test with diameters ranging from 1.66 cm to 6.76 cm. Six isolates that have the largest diameters clear zone, were tested for degradation of filter paper (Whatman no. 1). Isolates bacteria of B2S8 obtained from Bondowoso compost samples has the highest ability to degrade cellulose on Whatman paper no. 1 as much as 51.30%. Keywords: Compost, Isolation, Screening, Cellulolytic Bacteria.
Co-Authors Ida Bagus Wayan Gunam NYOMAN SEMADI ANTARA Yohanes Setiyo