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PENGARUH JENIS PELARUT DAN WAKTU MASERASITERHADAP KANDUNGAN SENYAWA FLAVONOID DAN AKTIVITAS ANTIOKSIDAN EKSTRAK DAUN ALPUKAT (Persea Americana Mill) Nico Kemit; I Wayan Rai Widarta; Komang Ayu Nocianitri
Jurnal Ilmu dan Teknologi Pangan (ITEPA) Vol 5 No 2 (2016): Jurnal ITEPA
Publisher : Department of Food Science and Technology Study Program, Faculty of Agricultural Technology, Udayana University

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Abstract

This research was conducted to determine effect of the kinds solvent and maceration time on flavonoid content and antioxidant activity of avocado leaf extract and to obtain maceration conditions that can produce flavonoid extract with the highest antioxidant activity. The experimental design used in this research was a randomize block design, which consisted of two factors. The first factor was the kinds of solvent, which consisted of 4 kinds namely aquades, acetone 90%, ethanol 90% and methanol 90%. The second factor was the time of maceration, which consisted of 4 level namely 18, 24, 30 and 36 hours. The treatment was repeated twice to obtain 32 units of the experiment. Data were analyzed with analysis of variance, followed by Duncan test. The results showed that the best treatment of avocado leaf extract is maceration by ethanol 90% for 30 hours which the highest resulted rendemen was 27,84%, total flavonoid was 64,12 mgQE/g dry weight sampel, antioxidant activity was 82,75%, and the IC50 value 417 mg/L.
STABILITAS SENYAWA FLAVONOID EKSTRAK DAUN ALPUKAT (Persea americana Mill.) TERHADAP PERLAKUAN pH DAN SUHU Nico Kemit; Mayun Permana; Pande Ketut Diah Kencana
Media Ilmiah Teknologi Pangan (Scientific Journal of Food Technology) Vol 6 No 1 (2019): Scientific Journal of Food Technology (March)
Publisher : Master Program of Food Science and Technology, Faculty of Agricultural Technology, Udayana University

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Abstract

This research aimed to get the etanol’s consentration and ratio avocado leaf to solvents on highestflavonoid content and antioxidant activity of avocado leaf extract and also get stability of pH andtemperature for flavonoid extract avocado leaf. This research did into two step. First step was effect ofetanol’s consentration and ratio avocado leaf to solvents. The experimental design used in this researchwas a completely randomized design with replication, which consisted of two factors. The first factorwas etanol’s concentration (90 and 96%). The second factor was ratio avocado leaf to solvents (1:6,1:8, 1:10). Data were analyzed with analysis of variance, followed by Duncan test. The best treatmenton first step would use into second step of this reseach. Second step was effect of flavonoid content’sstability of extract avocado leaf to pH and temperature. The experimental design used in this researchwas a completely randomized design with replication, which consisted of two factors. The first factorwas pH (4, 6 and 8). The second factor was temperature (70, 80 and 90OC). Data were analyzed withanalysis of variance, followed by Duncan test. The results showed that the best treatment in first stepwas etanol 90% and ratio avocado leaf to solvent 1:6 which the highest resulted rendemen, total fenol,total flavonoid and antioxidant activity were 17.54%, 79.19 mg/g extrak, 69.19 mg/g extrak and72.91%. The results showed that the best treatment second step was pH 4 and temperature 70OC whichthe best resulted total fenol, total flavonoid and antioxidant activity that stablest. Persentase that stillstay at extract were 94.9%, 86.99% dan 85.08%.