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STABILITAS SENYAWA FLAVONOID EKSTRAK DAUN ALPUKAT (Persea americana Mill.) TERHADAP PERLAKUAN pH DAN SUHU Nico Kemit; Mayun Permana; Pande Ketut Diah Kencana
Media Ilmiah Teknologi Pangan (Scientific Journal of Food Technology) Vol 6 No 1 (2019): Scientific Journal of Food Technology (March)
Publisher : Master Program of Food Science and Technology, Faculty of Agricultural Technology, Udayana University

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Abstract

This research aimed to get the etanol’s consentration and ratio avocado leaf to solvents on highestflavonoid content and antioxidant activity of avocado leaf extract and also get stability of pH andtemperature for flavonoid extract avocado leaf. This research did into two step. First step was effect ofetanol’s consentration and ratio avocado leaf to solvents. The experimental design used in this researchwas a completely randomized design with replication, which consisted of two factors. The first factorwas etanol’s concentration (90 and 96%). The second factor was ratio avocado leaf to solvents (1:6,1:8, 1:10). Data were analyzed with analysis of variance, followed by Duncan test. The best treatmenton first step would use into second step of this reseach. Second step was effect of flavonoid content’sstability of extract avocado leaf to pH and temperature. The experimental design used in this researchwas a completely randomized design with replication, which consisted of two factors. The first factorwas pH (4, 6 and 8). The second factor was temperature (70, 80 and 90OC). Data were analyzed withanalysis of variance, followed by Duncan test. The results showed that the best treatment in first stepwas etanol 90% and ratio avocado leaf to solvent 1:6 which the highest resulted rendemen, total fenol,total flavonoid and antioxidant activity were 17.54%, 79.19 mg/g extrak, 69.19 mg/g extrak and72.91%. The results showed that the best treatment second step was pH 4 and temperature 70OC whichthe best resulted total fenol, total flavonoid and antioxidant activity that stablest. Persentase that stillstay at extract were 94.9%, 86.99% dan 85.08%.
Karakteristik Asap Cair Batang Bambu Tabah (Gigantochloa nigrociliata BUSE-KURZ) Hasil Destilasi pada Suhu yang Berbeda Kadek Rahayu Swandewi; Pande Ketut Diah Kencana; Ni Luh Yulianti
Jurnal BETA (Biosistem dan Teknik Pertanian) Vol 8 No 1 (2020): April
Publisher : Program Studi Teknik Pertanian, Fakultas Teknologi Pertanian, Universitas Udayana, Badung, Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (335.789 KB) | DOI: 10.24843/JBETA.2020.v08.i01.p19

Abstract

Tujuan dari penelitian ini ialah agar mengetahui densitas, rendemen, serta senyawa kandungan kimia (pH, total asam, total fenol) yang terdapat didalam destilat asap cair hasil pirolisis batang bambu tabah pada suhu 125oC dan 150oC. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan faktor tunggal yaitu menggunakan suhu (suhu 125oC dan 150oC). Analisis sidik ragam menunjukkan jika perbedaan suhu antara 125oC dan 150oC berpengaruh nyata terhadap hasil analisis destilasi asap cair pirolisis bambu tabah. Adapun hasilnya ialah densitas tertinggi 1,0245 g/ml pada suhu 150oC dan terendah 1,02004 g/ml pada suhu 150oC, pH tertinggi 3,20 pada suhu 125oC dan terendah 3,14 pada suhu 150oC, total asam tertinggi 10,94% pada suhu 150oC dan terendah 7,70% pada suhu 125oC, total fenol tertinggi 0,65% ada suhu 150 oC dan terendah 0,61% pada suhu 125oC. Analisis beda nyata terkecil menunjukkan perbedaan suhu 125oC dan 150oC berpengaruh nyata terhadap pH, total asam, dan total fenol. Hasil menunjukkan adanya perbedaan karakteristik asap cair sebelum dan sesudah distilasi. Kata kunci: asap cair, bambu tabah, destilasi
Rebung Bambu Tabah (Gigantochloa Nigrociliata) Berpotensi Sebagai Bahan Afrodisiak pada Mencit Jantan (POTENTIAL APHRODISIAC ACTIVITY OF TABAH BAMBOO SHOOTS (GIGANTOCHLOA NIGROCILIATA) IN MALE MOUSE) Anak Agung Sagung Alit Sukmaningsih; Ida Bagus Wayan Gunam; Nyoman Semadi Antara; Pande Ketut Diah Kencana; I Wayan Widia
Jurnal Veteriner Vol 18 No 3 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (167.876 KB) | DOI: 10.19087/jveteriner.2017.18.3.393

Abstract

This study was conducted to observe the potential aphrodisiac activity of Gigantochloa nigrociliata in male mouse Mus musculus. The twenty four male mice, aged 12 weeks were divided randomly into four groups, each group consisted of 6 mice. One group was used as control (P0) where the mice were treated with aquadest. Three other groups were given treatment. The extracts contained of water (P1), etanol (P2), and n-hexane (P3). The animals in each treatment were treatments given 200 mg/kg bw Gigantochloa nigrociliata of extract orally (gavage) once daily as much as 0,2 mL for 33 days. The observations of sexual behaviour performed every three days during the treatment. Variables observed for the sexual behaviour are both the number and latency of mount and intromission. Mice were anaesthetized after 33 days. Cauda epidydimis were analyzed for motility, number and viability of sperms. The result showed that testosterone serum level increased significantly on etanol extract group (p<0.05). There were significantly increased the mounting number, intromission number with reduction in mounting latency, and intromission latency of male mice (P<0.05). There was significant an increase in the number of (a) sperm motility and number of sperm on etanol extract group (p<0.05). The results of the present study demonstrate that Gigantochloa nigrociliata extract improve sexual behaviour in male mouse. ABSTRAK Telah dilakukan penelitian mengenai potensi rebung bambu tabah sebagai bahan afrodisiak yang diujikan pada mencit jantan (Mus musculus). Sebanyak 24 mencit dikelompokkan menjadi empat perlakuan dan enam ulangan. Satu kelompok digunakan sebagai kontrol (P0). Tikus pada kelompok kontrol diberikan aquades. Tiga kelompok lainnya diberikan perlakuan ekstrak air bambu tabah (P1), ekstrak etanol bambu tabah (P2), dan ekstrak n-hexane bambu tabah (P3). Setiap hewan pada masing masing perlakuan diberikan ekstrak 200 mg/kg bb (bobot badan) sebanyak 0,2 mL ) secara oral selama 33 hari. Pengamatan perilakukawin berupa mount dan intromission dilakukan setiap 3 hari sekali. Setelah 33 hari hewan dikorbankan untuk dikoleksi epididimis bagian cauda yang digunakan dalam analisis spermatozoa dan pengambilan darah yang digunakan dalam analisis hormon testosteron serum. Hasil analisis dengan menggunakan uji one way Anova menunjukkan adanya perbedaan kadar hormon testosteron serum secara signifikan (p<0,05). Kelompok hewan dengan perlakuan etanol memiliki kadar hormon yang lebih tinggi dibandingkan kelompok lain. Kelompok hewan dengan perlakuan heksan memiliki kadar hormon terendah dengan perbedaan yang signifikan dibandingkan dengan kelompok perlakuan lain. Terjadi pula perbedaan motivasi seksual yang diamati pada perilaku kawin berupa mount dan intromission. Perbedaan jumlah dan waktu terjadinya mount dan intromission terjadi secara signifikan di antara kelompok perlakuan dan kontrol (p<0,05). Peningkatan jumlah mount dan intromission, serta semakin singkatnya waktu untuk mencapai mount dan intromission terjadi secara signifikan dibandingkan dengan kontrol dan diantara kelompok perlakuan (p<0,05). Hasil analisis data juga menunjukkan peningkatan motilitas spermatozoa tipe (a) yakni spermatozoa dengan gerakan yang progresif maju ke depan dan jumlah spermatozoa, terutama pada esktrak etanol rebung secara signifikan. Disimpulkan bahwa rebung bambu berpotensi sebagai bahan afrodisiak karena dapat meningkatkan hormon testosteron yang merupakan salah satu faktor penting dalam pengaturan sistem reproduksi serta dapat meningkatkan motivasi seksual yang diuji pada mencit jantan.