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All Journal Media Penelitian dan Pengembangan Kesehatan BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Makara Journal of Science
HEDDY JULISTIONO
Research Center for Biology Lembaga Ilmu Pengetahuan Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Public Health

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TOKSISITAS GLISEROL ATAU SUKROSA PADA SEL KHAMIR accharomyces cerevisiae YANG DISEMPAN PADA SUHU RENDAH BEKU Julistiono, Heddy
BERITA BIOLOGI Vol 5, No 4 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i4.1123

Abstract

The effect of cryoprotectants glycerol and sucrose on cell viability and fermentation rate of Saccharomyces cerevisiae after freezing (-30 °C) and Chawing (30 °C) were studied.Both freezing and thawing were done rapidly. The mortality of cells treated with low concentrations of cryoprotectans (2.5, 5, and 10 %) after 15 and 30 days of cryopreservation, was remarkably higher than that of control and higher concentration (20% and 40%).Glycerol or sucrose with concentration of 20 % and 40 % protected cells from severe mortality only after 90 days of cryopreservation.Fermentation rate of cells treated with 20 % or 40 % of the two cryoprotectants were higher than that of control after 60 and 90 days of cryopreservation.The data indicated that in certain circumstance cryoprotectant could be toxic for the cells during freezing and thawing.Since biomembrane is not permeable to sucrose, therefore we proposed that target of sucrose toxicity may be extracellular, whereas glycerol, which penetrate cells,targets of the toxicity may be both extracellular and intracellular domains.Interaction between cryoprotectant and cell membranes is discussed.
IDENTIFICATION OF YEASTS ISOLATED FROM GUNUNG HALIMUN NATIONAL PARK*[Identifikasi Khamir pada Taman Nasional Gunung Halimun] Kanti, Atit; Julistiono, Heddy; Sudiana, I Made
BERITA BIOLOGI Vol 6, No 1 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (596.655 KB) | DOI: 10.14203/bb.v6i1.1177

Abstract

Dua puluh sembilan isolat khamir diisolasi dari tanah Taman Nasional Gunung Halimun. Sumber isolat berasal dari batang pohon lapuk, akar lapuk yang diambil dari Gunung Botol, Cikaniki, dan Cipta Rasa untuk dipelajari aspek taksonominya. Berdasarkan atas karakter morfologi dan fisiologi, isolat-isolat tersebut digolongkan kepada kelompok ascomyceteous, basidiomyceteous dan imperfect khamir. Selanjutnya ketiga golongan tersebut dimasukkan ke dalam sepuluh kelompok (Kelompok 1 sampai X). Dari 29 isolat tersebut, 7 isolat dimasukkan ke dalam kelompok I diindentifikasi sebagai Debaryomyces hansenii, 6 dalam kelompok II sebagai Candida sp, 2 dalam kelompok III sebagai Pichia membranafaciens, 5 isolat dalam kelompok IV sebagai Candida galacta, 1 dalam kelompok V sebagai Candida sake, 4 dalam kelompok VI sebagai Cryptococcus humicolus, 1 dalam kelompok VII sebagai Rhodotorula minuta, 1 dalam kelompok VIII sebagai Candida sp, 1 dalam kelompok Candida sp, dan 1 dalam kelompok X dalam Candida sp. Macam sampel tampaknya tidak berpengaruh kepada keragaman jenis khamir seperti ditunjukkan oleh jenis yang sama diisolasi dari berbagai jenis sampel. Dari banyaknya jenis khamir yang diisolasi menunjukkan bahwa keragaman jenis khamir di Taman Nasional Gunung Halimun tergolong tinggi.
THE COMMUNITY OF SOIL YEASTS IN GUNUNG HALIMUN NATIONAL PARK Kanti, Atit; Sudiana, I Made; Julistiono, Heddy
BERITA BIOLOGI Vol 5, No 6 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (409.796 KB) | DOI: 10.14203/beritabiologi.v5i6.1081

Abstract

Fifty-two isolates were isolated from Gunung Halimun National Park on the basis of morphological and some physiological characteristics.Those isolates were belonged to three groups namely, ascomycetous, basidiomycetous and imperfect yeasts.Rhodotolum sp.was only found in Ciptarasa site at 1500 m asl, ascomycetous yeasts was only isolated from deteoretic root in Gunung Botol site, while Candida sp.(small globose shaped cells) was only isolated from soil at 1800 m asl of Gunung Botol site. Type of plant species appeared has no effect on yeasts diversity as shown by similar yeasts diversity was observed at rhizosphere soil of three dominating plant (Schima waallichii, Castanopsis javanica and Altingia excelsa) at Cikaniki study site.
KAJIAN KERAGAMAN GENETIK BURUNG KAKATUA TANIMBAR (CACATUA GOFFINI, FINSCH) MENGGUNAKAN PENCIRI "RAPD" Astuti, Dwi; Priyono, Siti N.; Julistiono, Heddy; Duryadi, Dedy
BERITA BIOLOGI Vol 4, No 2&3 (1998)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i2&3.1277

Abstract

The study was conducted to analyse genetic diversity of Goffini Finsch Cockatoo (Cacatua goffini Finsch) bird using RAPD (Random Amplified Polymorphic DNA) marker.PCR (Polymerase Chain Reaction) was performed on DNA samples extracted from 14 birds,using 18 random 10-merprimers and 2 random 12-merprimers. Fourteen out of 20 primers (70 %) successfully amplified DNAfragmens and 11 out of 14 primers (78,57 %) generated 1-2 specific alkies.The result clearly demonstrated that the RAPD marker allows for genetic diversity analyses of these birds in efficiently. Tree of relationship among 14 birds showed that there were two groups in the population ofGoffin 's Cockatoo.
SUPEROXIDE DISMUTASE ACTIVITY AND ETHANOL RESPIRATION IN A FUNGI RESISTANCE TO ETHANOL MONASCUS SP. MM Julistiono, Heddy; Suharna, Nandang; Desnora, Beni
JURNAL BIOLOGI INDONESIA Vol 3, No 4 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v3i4.3319

Abstract

ABSTRACTMonascus sp. MM was a contaminant fungus isolated from museum specimenpreserved with ethanol 70 %. In order to verify role of superoxide dismutase (SOD) inprotecting cell from ethanol toxicity during ethanol metabolism, SOD activities of Monascussp.MM and a Monmcus sp. NGK, which was isolated fiom fermented red rice (angkak), werecompared. When fungus was grown with glucose, CuIZn-SOD activity of Monascus sp., MMwas 7.1 times of thht of Monascus sp. NGK. Whereas in ethanol medium, CuIZn-SOD activityof Monarcus sp. MM was 24.6 times of that in Monascus sp. NGK. Induction of CuIZn-SODMonmcus sp. MM by ethanol was not observed. Compared with Mn-SOD, activity of CuIZn-SOD was markedly important (I 0 times of Mn-SOD when fungi grown with ethanol; 12 timeswhen the fungi grown with glucose). The data indicated that Cu/Zn-SOD might play animportant role in protecting cell fiom ethanol toxicity during ethanol metabolism. Ethanolrespiration rate of Monascus sp. MM was also important since O2 consumption and ethanoldegradation rates were clearly higher than that of Monascus sp. NGK.Keywords: Monarcw sp., superoxide dismutase, respiration, ethanol resistance.
EKSPLORASI KEANEKARAGAMAN AKTINOMISETES TANAH TERNATE SEBAGAI SUMBER ANTIBIOTIK Nurkanto, Arif; Listyaningsih, Febrianti; Julistiono, Heddy; Agusta, Andria
JURNAL BIOLOGI INDONESIA Vol 6, No 3 (2010): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v6i3.3141

Abstract

ABSTRACTExploration of Soil Actinomycetes Diversity from Ternate as Indigenous Antibiotic Sources.Actinomycetes of soil samples from Ternate, North Moluccas were isolated using SDS-YEmethod in humic acid vitamin agar. Ternate has high abundance of Actinomycetes, approximately6.00 ? 487 x 104 CFU/ g soil, depends on habitat types. We have selected 60 isolates andconducted antibiotic screening against pathogenic bacteria and fungi using agar diffusionmethod and found both narrow and broad antibiotic spectrum types . Based on 16S rDNAanalysis, all Actinomycetes with antibiotic activities are belong to the genus Streptomyces. .Minimum Inhibitor Concentration (MIC) value was determined by broth microdilution method.It was found that MIC values varied, depended on microbial tested. We found two isolateswith higher antibiotic activity compared to the commercial antibiotics (chloramphenicol,erythromycin for antibacterial and nystatin, kabicidin for antifungal). Cell destruction analysiscaused antibiotic activities was conducted through leak of protein and nuclatic acid.Key words : Actinomycetes, soil, Ternate, antibiotic, cell distruction
INDUCTION OF SUPEROXIDE DISMUTASE ACTIVITIES AND ETHANOL RESISTANCE BY SODIUM CHLORIDE AND LEAD ACETATE IN YEAST CANDIDA SP. Y390 Julistiono, Heddy
JURNAL BIOLOGI INDONESIA Vol 4, No 1 (2006): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v4i1.3271

Abstract

ABSTRAKAktivitas Induksi Superoksida Dismutase dan Resistensi Etanol oleh Sodium Kloridadan Plumbum Asetat pada Khamir Candida sp. Y390. Untuk mengetahui sifat khamirCandida sp. Y390 dalam melindungi diri dari cekaman oksidatif, telah dilakukan risetkajian toleransi sel terhadap etanol dan hubungannya dengan enzim superoksida dismutase(SOD) pada khamir tersebut. Viabilitas sel yang ditumbuhkan pada media gliserol dandiinkubasi pada etanol 17,s % selama 1 jam adalah 0.30 f 0.09 % , pada media gliseroldan 1 1,7 % natrium khlorida adalah 1.65 f 0.5 % sedang pada media giserol dan 1 ppmplumbum asetat adalah 1.16 f 0.7 %. Aktivitas CuZnSOD pada sel yang diinduksi olehnatrium khlorida atau plumbum asetat turun sedikit, yakni masing-masing 1,7 dan 1,9kali. Aktivitas MnSOD pada sel yang diperlakukan dengan natrium khlorida naik sedikit(sekitar 1,03 kali), namun pada sel yang diperlakukan dengan plumbum asetat naik secaramenyolok (sekitar 3,6 kali). Data ini menunjukkan bahwa metabolisme etanol dapatmenyebabkan Candida sp. Y390 mengalami cekaman oksidatif. MnSOD khamir mungkinberperan dalam melindungi sel dari kerusakan akibat cekaman oksidatif.Keywords: Candida sp. Y390, yeast, ethanol metabolism, oxidative stress, SOD.
PEROKSIDASI LIPID OLEH PARASETAMOL DAN EKSTRAK AIR PANAS TEH HIJAU (CAMELLIA SISNENSIS ) PADA SEL KHAMIR CANDIDA TROPICALIS YANG DI SIMPAN PADA SUHU RENDAH Julistiono, Heddy
JURNAL BIOLOGI INDONESIA Vol 8, No 1 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v8i1.3070

Abstract

ABSTRACTLipid Peroxidation by Paracetamol and Hot Water Extract of Green Tea (Camellia sinensis ) in Yeast Candida tropicalis preserved in Low Temperature. The use of C. tropicalis cell as a tool to evaluate antioxidant property of green tea to protect oxidative stress caused by paracetamol in cell level was investigated in our laboratory. Immediate availability of cell culture will significantly reduce the time of cell preparation. Low temperature preservation of cell culture is one of methods to produce cell cultures. However, low temperature might affectphysiological state of the cell. In this study, effect of low temperature preservation (4 ºC, 10 days) on the oxidative response of yeast cell treated with paracetamol and hot water extract of green tea had been investigated. Cells incubation with paracetamol 0.3 % for 2 h caused oxidative stress in both fresh and preserved culture since the content of a marker of oxidativestress, peroxyd lipids increased significantly. Whereas, concentration of peroxidised lipids in preserved cultures was lower than that of fresh culture. Increasing of peroxydized lipids followed with decreasing of cell viability in fresh culture but not in preserved culture. Green tea withconcentration of 0.1 % decreased peroxyd lipids in fresh cultures treated with paracetamol but not in preserved cultures. Green tea with concentration of 0.2 % or 0.4 % in increased peroxyd lipids in fresh cultures treated with paracetamol but not in preserved cultures. The data indicated that green tea did not show anti- or pro-oxidative effect in cultures preserved in low temperature treated with paracetamol. However, induction of super oxide dismutase (SOD), an antioxidant defense enzyme, was not observed in cell preserved in low temperature. The study revealedthat low temperature might make the cell more resistant against prooxidative properties of paracetamol.Keywords: C. tropicalis, oxidative stress, low temperature, paracetamol, green tea
Antifungal Activity of (+)-2,2’-Epicytoskyrin A and Its Membrane-Disruptive Action Wulansari, Dewi; Praptiwi,; Julistiono, Heddy; Nurkanto, Arif; Agusta, Andria
Makara Journal of Science Vol. 20, No. 4
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

(+)-2,2’-Epicytoskyrin A, a bis-anthraquinone isolated from fungal endophyte Diaporthe sp. GNBP-10 associated with Uncaria gambir Roxb., was investigated for its antifungal activity. The broth microdilution method was used to determine the minimum inhibitory concentration (MIC) against 22 yeast strains and three filamentous fungi. The MICs of (+)-2,2’-epicytoskyrin A ranged from 16 to 128 µg/mL, which exhibited lower activity than the antifungal nystatin. A study of the mechanism of action revealed similar effects of (+)-2,2’-epicytoskyrin A and nystatin on Candida tropicalis at their MICs (16 and 8 µg/mL, respectively) and 2 times of the MIC. Both compounds caused cytoplasmic material and ion leakages on fungal cell, which were characterized by an increase in absorbance at 260 nm and 280 nm as well as Ca2+ and K+ ion concentrations. The morphology of the fungal cells after (+)-2,2’-epicytoskyrin A treatment was observed under a scanning electron microscope. The control cells, which were not treated with either (+)-2,2’-epicytoskyrin A or nystatin, showed a smooth surface, while the cells treated with either (+)-2,2’-epicytoskyrin A or nystatin shrank and displayed a donut-like shape. More shrinkage was observed in the 2 times MIC concentration and even more in the cells exposed to nystatin. The action of (+)-2,2’-epicytoskyrin A was proposed through membrane disruption.
Co-Authors Adam Adam ANDRIA AGUSTA Arif Nurkanto Atit Kanti Desnora, Beni Desnora, Beni DEWI WULANSARI Dwi Astuti Hanjono, Sukamto I Made Sudiana Listyaningsih, Febrianti Listyaningsih, Febrianti Muthmainah, Resti Siti Nandang Suharna, Nandang Praptiwi, Priyono, Siti N. Saragih, Ernawati TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Yulineri, Titin Yulineri, Titin