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ISOLASI PROTEIN DAN PRODUKSI KONSENTRAT PROTEIN DAUN (KPD) SEBAGAI SUPLEMEN PAKAN TERNAK Yatno Yatno; Suparjo Suparjo; Rasmi Murni
Pastura : Jurnal Ilmu Tumbuhan Pakan Ternak Vol 7 No 2 (2018): Pastura Vol. 7 No. 2 Tahun 2018
Publisher : Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1493.158 KB) | DOI: 10.24843/Pastura.2018.v07.i02.p07

Abstract

The research aim to determine the effect of extraction using various combination of pH alkaline-acid to protein and amino acid content of Lamtoro Protein Concentrate Leaf (LPCL). Completely Randomized Design (CRD) with 3 treatments and 5 replications. The Lamtoro Leaf Meal (LLM) (50 gram) was blended in NaOH (200 ml) for 15 menit and centrifuged to separate the supernatant from extracted LLM. The extracted LLM was re-extracted with buffer acetat pH 4,6 and sentrifuged again to separate supernantant from extracted LLM. Both supernatant from NaOH and buffera acetat pH 4,6 extraction were mixed and precipitated with buffer phospat until isoelectric point. The treatments were tested consisted of extracted with NaOH pH 8, NaOH pH 9 and NaOH pH 10. The parameters measured; isoelectric point at precipited, crude protein, total amino acid (TAA), total essential amino acid (TEAA) and individual amino acid. The results showed that the precipited obtained isoelectric point pH 7 was 2.66; 1.03 and 2.58 gram at the extraction treatments using NaOH at pH 8, 9 and 10, respectively. LPCL extracted using NaOH at pH 9 and 10 contained a high crude protein of 54.82 and 52.60% higher than the extraction using NaOH at pH 8 of 46.98%. The total amount of amino acid and essential amino acid (AAE) of LPCL were highest in treatment using NaOH at pH 10 of 26,58; 13.31 %, respectively. Combination extraction LLM using pH buffer 10 followed by re-extract using acetate buffer pH 4,6 contains crude protein and good essential amino acid, especially leucine. Conclution of the research that the combination extraction using NaOH at pH 10 and followed by re-extract using acetate buffer at pH 4.6 is the best treatment contain crude protein and essential amino acid at LPCL. Keywords : Crude protein, essential amico acid, extraction.
Estimasi Kebutuhan Kalsium Dan Fosfor Ayam Arab Betina Fase Pre-Laying Pada Sistem Pemberian Pakan Bebas Pilih Aldi Irawan Tarigan; Syafwan; Rasmi Murni
Humantech : Jurnal Ilmiah Multidisiplin Indonesia Vol. 2 No. Special Issue 2 (2022): Humantech : Jurnal Ilmiah Multidisiplin Indonesia
Publisher : Program Studi Akuntansi IKOPIN

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Arab chickens are descendants of Kriel-Silver Brakel Chickens from Belgium and Indonesian local laying hens. For generations, arab chickens have been delegated as unrivaled arab chickens, because they have a high egg production capacity. This study aimed to calculate the calcium and phosphorus requirements of hens in the pre-laying phase. This study was conducted using a Randomized Block Design (RBD) with 4 groups and each group was repeated 4 times. Each group consists of 9 to 10 birds. The treatments applied were as follows: T1; Control feed, T2; High calcium low phosphorus feed (HCLP) and low calcium high phosphorus feed (LCHP), T3; High calcium high phosphorus (HCHP) feed, high calcium low phosphorus feed (HCLP) and low calcium high phosphorus feed (LCHP). P4: Control feed, high calcium high phosphorus (HCHP) feed, high calcium low phosphorus feed (HCLP), and low calcium high phosphorus (HCLP) diet. The observed variables were feed consumption, calcium and phosphorus consumption and calcium and phosphorus concentrations. The data obtained were analyzed using variance (ANOVA) and if the treatments had a significant effect, it was continued with the Duncan Multiple Range Test. The results showed that the treatments had a significant effect on all observed variables, namely feed consumption, calcium consumption, phosphorus consumption, calcium concentration and phosphorus concentration (P<0.05). It was concluded that the concentration of Ca (2.91-2, 97%) and P (0.51-0.52%) of the feed consumed by Arabic hens with free-choice feeding method in the pre-laying phase were higher than the concentration of Ca (2.70%) and concentration of phosphorus (0.48%) used in the standard Hy-line phase brown chicken pre-laying phase.