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Daun Binahong (Andredera cordifolia Steenis) Sebagai Alternatif Insektisida Terhadap Miasis yang Disebabkan Lalat Chrysomya bezziana (ANREDERA CORDIFOLIA STEENIS (BINAHONG) LEAF AS AN ALTERNATIVE INSECTICIDE AGAINST CHRYSOMYA BEZZIANA CAUSED MYIASIS) Ietje Wientarsih; Aulia Andi Mustika; April Hari Wardhana; Dodi Darmakusumah; Lina Noviyanti Sutardi
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (103.219 KB) | DOI: 10.19087/jveteriner.2017.18.1.121

Abstract

Larvae Chrysomya bezziana caused myiasis in most livestock in Indonesia. Drugs of choice for treating myasis are synthetic insecticides. Unfortunately the insecticides has negative effect on animal product, killed insect non target and insect resistenst. The research was conducted on the activity of ethanol extract of binahong leaves against various stages of C. bezziana larvae (L1, L2, and L3). Five treatments group tested were: group without any treatment (negative control), groups treated with ethanol extracts of binahong leaves 0.5%, 1%, and 2%, respectively, positive control were given coumaphos. The treatments were applied with five replications. Number of living larvae and pupae, pupae weight and number of imago were observed. The results demonstrated that 0.5% of the extract effective consentration which was able to kill the larvae (L1 and L2). Finally 2% of the extract was the most effective concentration which was able to kill larvae (L3) and decrease the pupae weight. Ethanol extract of binahong leaves was significantly able to reduce the growth of C. bezziana larvae due to contact and digestive effect of the active compounds contained in Anredera cordifolia leaf. ABSTRAK Miasis merupakan infestasi larva lalat yang terdapat pada jaringan hidup. Penyakit ini umum menyerang hewan maupun manusia. Obat yang digunakan sebagai pengendalian kasus miasis di lapangan sampai saat ini sangat terbatas dan menggunakan insektisida sintetik. Penelitian ini bertujuan melihat aktivitas ekstrak etanol daun binahong terhadap larva lalat Chrysomya bezziana pada stadium L1, L2, dan L3. Penelitian dibagi menjadi lima kelompok perlakuan dengan lima ulangan. Kelompok perlakuan terdiri dari kontrol negatif (tanpa pemberian obat), kelompok perlakuan yang diberikan ekstrak binahong dengan konsentrasi berturut-turut 0,5%, 1%, 2%, dan kelompok yang diberikan coumaphos (kontrol positif). Peubah yang diamati adalah jumlah larva yang hidup dan berkembang menjadi pupa, bobot pupa, dan daya tetas pupa menjadi imago. Hasil penelitian menunjukkan bahwa ekstrak etanol daun binahong pada konsentrasi 0,5% mempunyai efikasi sebagai larvasidal terhadap L1 dan L2 serta pada konsentrasi 2% merupakan konsentrasi terbaik sebagai larvasidal L3 larva C. bezziana. Ekstrak etanol daun binahong memiliki kemampuan menghambat larva lalat C. bezziana akibat dari efek cerna dan efek kontak bahan aktif yang terkandung di dalam daun binahong.
Aktivitas Antibakteri Ekstrak Etanol dan Destilat Jahe dan Sirih terhadap Mycoplasma gallisepticum dan Escherichia coli (ANTIBACTERIAL ACTIVITY OF ETHANOL EXTRACT AND DESTILAT OF GINGERS AND PIPER BETLES AGAINST MYCOPLASMA GALLISEPTICUM AND ESCHERICHIA CO Min Rahminiwati; Aulia Andi Mustika; Agung Zaim; Lina Novianti Sutardi
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (106.762 KB)

Abstract

Betle and ginger juice extracts have been reported to have antibacterial activity against E. coliand M. gallisepticum. However, fractionation analysis showed the have differences in their antibacterialpotency which appeared to be associated with the nature of the solvent polarity. This study wasconducted to obtain information about antibacterial activity of Piper betles and gingers extract againstE. coli and M. gallisepticum. Three types of betle, P. betle, P. betle var nigra, P. crocatum, wereexamined for their antibacterial activity using disc method and three types of ginger, Zingiber officinale,Z. officinale Linn var rubrum, and Z. majus rumph which were extracted by soxhletation using ethanol30%, 60% and 90% as well as distestillation for three and five hours. Piper betle and P. crocatumconsistently have antibacterial effect against E. coli whereas Z. officinale consistently has antibacterialeffect against M. gallisepticum, either extracted by distillation or soxhletation. Piper betle is potentialyield of distillate and extract that has the highest antibacterial activity against E. coli, and M.gallisepticum with inhibitory zone were 9.76 mm and 22 mm respectively.
The Effects of High Fat Diets on Body Weight and Lipid Profile Changes of Sprague-Dawley Rats Lailan Safina Nasution; Aulia Andi Mustika; Andriyanto Andriyanto
Buletin Farmatera Vol 7, No 1 (2022)
Publisher : Universitas Muhammadiyah Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30596/bf.v7i1.9336

Abstract

Background: Lipids come in from many different sources in one’s diet. In this experiment, we intend to find out the kind of lipids that causes the highest increase in rat body weight and the most influence in the lipid profiles. Method: We compared three kinds of lipid: beef fats, egg yolk and palm oil which were added to the rats’ standard diet. Twenty-eight 20-24 week-old male Sprague-Dawley rats weighing 250–300 g were randomly divided into 4 treatment groups given a high beef fat diet (BF), a high egg yolk (EY), a high vegetable fat (VF), and a standard chow diet (SD) as control for 30 days. The body weight of each rat was monitored weekly and the lipid profiles were measured enzymatically at the end of the experiment. Result: The EY and VF body weights increased significantly compared to the SD (p<0.05). The total cholesterol level of BF, EY and VF increased significantly compared to the SD groups (p<0.001). The triglycerides level of BF, EY, and VF increased significantly compared to SD (p<0.001). The HDL-cholesterol levels were significantly lower in BF, EY and VF compared to SD (p<0.05). The LDL-cholesterol in BF, EY and VF were significantly increased compared to SD (p<0.05). Conclusion: High egg yolk diet causes a bigger increase in rat body weight, LDL levels, cholesterol levels, and triglycerides than the high beef fat diet and high vegetable fat diet compared to control. It also causes lower HDL levels than the other diet groups compared to control.
Peningkatan Produktivitas Ayam Petelur Melalui Pemberian Ekstrak Etanol Daun Kemangi (INCREASED LAYING HENS PRODUCTIVITY THROUGH THE ADMINISTRATION OF ETHANOL EXTRACT OF KEMANGI LEAVES) Andriyanto .; Ridi Arif; Mohammad Miftahurrohman; Yayuk Sri Rahayu; Erli Chandra; Alifiana Fitrianingrum; Risna Anggraeni; Diah Nugrahani Pristihadi; Aulia Andi Mustika; Wasmen Manalu
Jurnal Veteriner Vol 15 No 2 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Empirically, kemangi leaves reported to increase health quality in human and livestock. Thepreliminary study was designed to explore the potency of ethanol extract of kemangi leaves to increaselaying hens performance. Sixteen laying hens (pullet) were divided into 4 groups and repeated 4 times.Control group was laying hen administered aquadest orally, treated group was laying hen administeredextract of kemangi leaves orally at a dose of 1, 2, and 3 mg/kg BW, respectively. Every day, the experimentallaying hens were fed for 3 times and drinking water was provided ad libitum. Variables observed were thenumber of eggs, egg weight, time of first laying, egg laying intervals, egg quality ( water content, crudeprotein, and crude fat), and liver function (SGPT and SGOT values) . Results of this research showed thatadministration of kemangi leaves extract at a dose of 3 mg/kg BW significantly increased the number ofegg production and egg weight (p<0.05). Time of first laying and laying interval did not show any significantdifference among treatments. Examination of moisture, crude protein, and crude fat content of the eggindicated that the administration of kemangi leaves extract did not affect egg quality. Extract of kemangileaves decreased SGPT and SGOT values that indicated improvement of liver function. It was concludedthat administration of ethanol extract of kemangi leaves could increase laying hens productivity byimprovement of liver function that is critical in vitellogenesis.
Pemberian Pregnant Mare Serum Gonadotropin Sebelum Perkawinan dan Jamu Selama Kebuntingan untuk Memperbaiki Performa Anak Domba (ADMINISTRATION OF PREGNANT MARE SERUM GONADOTROPIN AND JAMU DURING GESTATION TO IMPROVE FOR LAMB PERFOMANCE) Andriyanto .; Ridi Arif; Adi Winarto; Leo Sapelani Soinbala; Bondan Achmadi; Aulia Andi Mustika; Diah Nugrahani Pristihadi; Amrozi .; Wasmen Manalu
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (104.086 KB)

Abstract

Administration of pregnant mare serum gonadotropin (PMSG) hormone prior to mating increasesquality of lamb born on one and two litter sizes, but in three litter size the motality is higher. Administrationof traditional herbal medicine “jamu” consisting of ginger,green chiretta, cinnamon, Zingiberzerumbet, andpepper during gestation increases performance of lambs. This research was conducted to explore theeffectivity of PMSG injection prior to mating and jamu administration during gestation to increase lambsperformance. Eighteen priangan fat-tail ewes with weight around 20-25 kg were injected with PGF2á at adose of 10 mg/ewe twice with 11 days interval to synchronize estrous cycle. Injection of PMSG at a dose of200 IU/ewe was conducted at the second PGF2á injection. The experimental ewes were mated naturally anddivided into a randomized design with a 2 x 3 factorial arrangement with three replications. The firstfactor was dose of PMSG with two levels i.e., 0 and 200 IU/ewe. The second factor was dose of jamu withthree levels i.e., 0, 15, and 30 mL/ewe. Jamu was administered orally every week during gestation. Injectionof PMSG and administration of jamu decreased prenatal mortality, increased ratio of lamb per ewe, increased total lamb born with average birth weight and total birth weight were higher in jamu at a doseof 15 and 30 mL/ewe by 30.02 and 31.76%, repectively. During the first month postnatal, lambs born toewes injected by PMSG and administered jamu had higher number of lambs survive, average weight, totalweight around 3 times as compared to control. It was concluded that injection of PMSG prior to mating andadministration of jamu during pregnancy increase lamb birth weight and improve the quality of lamb.
Improving the Quality of Oocytes of Old and Productive Ages White Rats (Rattus norvegicus) Using Pregnant Mare Serum Gonadotrophin Sultania A Suleman; Andriyanto Andriyanto; Aulia Andi Mustika; Wasmen Manalu
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 3 No. 1, JANUARY 2019
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v3i1.5627

Abstract

Abstract This study was conducted to improve the quality of oocytes in old-female rats by using pregnant mare serum gonadotrophin (PMSG). Female rats at productive age were used as a control.  The experimental rats were injected with 4 doses of PMSG i.e., 0, 2.5, 5.0, and 10 IU PMSG. After 2 weeks of acclimation to the experimantal condition, the experimental rats were injected with PGF2α at a dose of 25µg/g BW two times with 2 days interval to synchronize estrous cycle. PMSG injections were conducted at the same time with the second PGF2α injection.  After PMSG injection, the experimental rats were divided into two groups rats, i.e., rats without mating for masurement of oocyte qualities and rats mated for measurement of offspring qualities.  Therefore, 16 experimental rats from each age group were sacrifized for maeasurement of oocyte qualities.  The other 16 experimental rats for each age group were mated for measurement of offspring qualities. Parameters measured were hematological profile, uterine and ovarian weights, the qualities of oocytes, the qualities of the offsspring born by using swimming test and rat maze test. The collected data were analyzed by analysis of variance (ANOVA) and continued with Duncan test with a 95% confidence level. The data were analyzed using SSPS. The results showed that the improvement in the quality of oocytes in old female rats using the PMSG hormone in this study showed an increase in the quality of oocytes in old and productive age rats. The highest number of oocyte quality was found in rats of productive age (3.25) and significantly different (P< 0.05) from the other oocyte qualities. The quality of offspring born to old age and productive age rats injected with PMSG were improved.  It was concluded that the improvement of oocyte quality by using PMSG also improves offspring qualities.