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Wiwin Winarsih
Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali

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Aktivitas Salep Ekstrak Rimpang Kunyit dalam Proses Persembuhan Luka pada Mencit yang Diinduksi Diabetes (THE ACTIVITY OF TURMERIC EXTRACT OINTMENT IN THE WOUND HEALING PROCESS OF INDUCED DIABETIC MICE) Wiwin Winarsih; Ietje Wientarsih; Lina Noviyanti Sutardi
Jurnal Veteriner Vol 13 No 3 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this study was to evaluate the effectiveness of ethanol turmeric extract ointment in woundhealing process of streptozotocin-induced diabetic mice. Ethyl acetate and hexane fractions of ethanolturmeric extract were studied for their wound-healing properties in the formulation of ointment. Sixty micewere divided into 4 groups : group I was control (normal control, without treatment); group II was diabeticgroup (diabetic, without treatment); group III was diabetic and treated with ethyl acetate fraction ointment,group IV was diabetic and treated with hexane fraction ointment. The diabetic groups were i.p injectedwith 40mg/kg of streptozotocin and all groups were received incision 2 cm on their back skin. The ointmentsof ethyl acetate and hexane fraction were given topically twice a day. Three mice from each groups werenecropsied at 2nd, 4th, 7th, 14th and 21st days post incision (pi) for gross pathological and histopathologicalevaluation of the injured skin. Gross examination revealed that the ethyl acetate and hexane fractionointment groups showed better result on wound-healing process compared to the diabetic group.Microscopically, the ethyl acetate and hexane fraction ointment groups showed faster neovascularizationand reepithelialization compared to the diabetic group. In comparison with the diabetic group, the ethylacetate and hexane fraction ointment groups showed fewer neutrophils infiltration which indicated antiinflammatory activities of ethyl acetate and hexane fractions. Based on the macroscopic and microscopicobservation, the ointments of ethyl acetate and hexane fraction have properties to promote wound healingin diabetic mice.
Uji Toksisitas Akut Ekstrak Rimpang Kunyit pada Mencit : Kajian Histopatologis Lambung, Hati dan Ginjal (ACUTE ORAL TOXICITY OF TURMERIC EXTRACT IN MICE : HISTOPATHOLOGICAL STUDIES OF STOMACH, LIVER AND KIDNEY) Wiwin Winarsih; Ietje Wientarsih; Nova P. Sulistyawati; Istifharany Wahyudina
Jurnal Veteriner Vol 13 No 4 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This experiment was carried out to determine the mouse lethal dose (MLD50 of ethanolic extract ofthe turmeric (Curcuma longa) rhizomes following single oral administration and to evaluate thetoxicopathologic effects of the extract in certain organs ie stomach, liver and kidney. Forty five male micewere divided into nine groups. Four groups were treated orally with ethyl acetate fraction, four groups weretreated orally with hexane fraction of ethanolic turmeric extract, and one group was used as control. Theethyl acetate fraction groups were administered orally with the ethyl acetate fraction at doses of 7.5, 1.5,30 and 60 g/kg body weight. The hexane fraction groups were administered orally with the hexane fractionwith the dosage of 7,5, 15, 30 and 60 g/kg body weight. The control group received normal saline. MLD50 ofethyl acetate fraction was 27,98 gram/kg body weight by per oral administration. Oral MLD50 of hexanefraction was 19,50 gram/kg body weight. Histopathological features of the ethyl acetate and hexanefractions groups showed increased amount of parietal cells in stomach and parenchymal degeneration andnecrosis in their liver and kidney.