Juniastuti Juniastuti
Department of Microbiology, Faculty of Medicine/Airlangga University, Soetomo Hospital, Surabaya.

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The Prevalence of Human Immunodefiency Virus-1 (HIV-1) Subtypes and Transmission Method among HIV/AIDS Infection Patient in Tulungagung, East Java Indonesia Nasronudin, Nasronudin; Lusida, Maria Inge; Witaningrum, Adiana Mutamsari; Kotaki, Tomohiro; Khairunisa, Siti Qamariyah; Utomo, Budi; Widiyanti, Prihartini; Rahayu, Retno Pudji; Juniastuti, Juniastuti; Qushay, M.; Ardianto, Achmad
Indonesian Journal of Tropical and Infectious Disease Vol 5, No 5 (2015)
Publisher : Institute of Topical Disease

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The rapid epidemic growth of HIV is continuing in Indonesia. There are some factors which have influenced the spreading of this epidemic in Indonesia, such as the poor awareness to avoid unsafe free sex attitude and the sharing of needles and syringes among intravenous drug users (IDUs). The sexual transmission of HIV has also apparently increased in Tulungagung. Commercial sex workers play a significant role in the spread of HIV in Tulungagung. People in Tulungagung have worked at other countries as Indonesian migrants. This condition can cause the increase number of HIV-1 case and the possibility of genetic variation (subtype) HIV-1 in Tulungagung. This research is aimed to analyze the subtype and to determine estimation of transmission mode on infected patient of HIV-1 and AIDS who came to Seruni clinic Dr. Iskak hospital in Tulungagung. 40 HIV?AIDSpatients were interviewed to determine the subtype and the transmission mode. The results showed that 14 of 40 plasma samples (35%) were successfully to amplified and sequenced. OverallCRF01-AE wereidentified as predominant subtype among HIV/AIDS patients in Tulungagung. Based on individual information, 31 of 40 subjects (77%) were heterosexual transmission.
HEPATITIS B SEROLOGY PROFILES ON CHILDREN AGED 1–13 YEARS OLD IN SUMENEP, MADURA Lusida, Maria I; Utsumi, Takako; Purwono, Priyo B; Amin, Mochamad; Marcia, Dian; Firdarini, Itja; Juniastuti, Juniastuti; Putera, Edward M
Indonesian Journal of Tropical and Infectious Disease Vol 3, No 2 (2012)
Publisher : Institute of Topical Disease

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Background: Hepatitis B virus (HBV) which was acquired during perinatal or childhood would promote hepatocellular carcinoma with even higher percentage than that which was acquired during adult age. That is why HBV represents a serious public health threat for children. HBV vaccination has been integrated into national expanded programme on immunization (EPI) since 1997. The aimof they study is to investigate the prevalence of HBV among children who were born after 1997 in Sumenep. Material and Methods: a total of 102 children who were born after 1997 were enrolled in this study. All children were admitted in the Emergency Room and Pediatric Ward of dr. H. Moh Anwar General Hospital for some reasons. Written informed consents were obtained from parents/guardians of all the children. Study protocol was reviewed and approved by the Ethics Committees. All of these cases were examined for hepatitis B surface antigen (HBsAg), antibody to HBsAg (Anti-HBs), and antibody to hepatitis B core antigen (Anti-HBc). Result and Discussion: Overall, 6 (5.88%) of 102 samples were positive for HBsAg, 51 (50.00%) of 102 samples were positive for anti-HBs, and 49 (48.04%) of 102 samples were positive for anti-HBc. All the children were born after 1997. Conclusion: HBsAg rate is still high even after universal vaccination program, acquired protective antibodies against hepatitis B surface antigen were sufficient, but there is a suspicion for occult hepatitis B infections (OBI). A further study to confirm OBI is needed.
CORRELATION OF ROUTINE URINE CULTURE AND STONE CULTURE TO POST-OPERATIVE SIRS Indrawan, Taufik; Hardjowijoto, Sunaryo; Soebadi, Doddy M.; Juniastuti, Juniastuti; Budiono, Budiono
Indonesian Journal of Urology Vol 21 No 1 (2014)
Publisher : Indonesian Urological Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32421/juri.v21i1.21


Objective: This study evaluated the correlation between preoperative urine culture and intraoperative stone culture and the impact of stone culture findings on post-operative systemic inflammatory response syndrome. Material & Method:Patients with kidney stones who underwent percutaneous nephrolithotomy (PCNL) from February to May 2012 were prospectively analyzed. A pre-operative urine culture was obtained in the morning before the operation, fragmented stone collected were cultured in Departement of Microbiology. Patients were monitored closely in the postoperative period for signs of systemic inflammatory response syndrome (SIRS). Results:A total of 33 patients underwent PCNL and examined for urine cultures, stone culture and postoperative SIRS, 15 (45.45%) patients with positive urine culture, 18 patients (54.54%) with positive stone culture but only 1 patient (3.03%) had same pathogen (p = 0.629). Ten patients (55.6%) with positive stone cultures had evidence of systemic inflammatory respose syndrome postoperatively. The calculated stone culture value for sensitivity, specificity, positive predictive value and negative predictive value were 100%, 65.2%, 55.6%, and 100%. Preoperative hydronephrosis (p = 0.003) and operative time (p = 0.001) are identified as the key risk factors for SIRS after PCNL.Conclusion: Positive stone culture are better predictors for SIRS after PCNL. Stone culture examination is an essential in directing the proper antibiotic therapyin patients with SIRS after PCNL.  Keywords: Percutaneous nephrolithotomy (PCNL), urine culture, stone culture, systemic inflammatory response syndrome after PCNL.