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Abundance and Diversity of Bacteria on Catfish Nursery Rearing (Clarias gariepinus) With Closed Water System (Close Water System) Azhar, Muhammad Hanif; Ulkhaq, Mohammad Faizal; Prayogo, .
Journal of Aquaculture Science Vol 2, No 1 (2017): Journal of Aquaculture Science
Publisher : Airlangga University

This research aimed to identify types of bacteria existed in nursery rearing pond of catfish (Clarias gariepinus) using closed water system. The observed data include bacterial abundance and diversity in the water and in the fish digestion system, fish growth and water quality. This research was performed in the media of 3 concrete pools with the size of 3 x 1 x 0.8 m3 containing fish stock spreading of 3000 tail / pond. The obtained results showed that the highest abundance of bacteria was found in the surface and the bottom of pond C with the abundance number ofÂ Â 3,0 x 104and 1,2 x 103for aerobic and anaerobic bacteria, respectively. Moreover, it was figured that the dominant bacterial species found in the water and the gastrointestinal tract were from the genus of Bacillus, Clostridium and Lactobacillus. Meanwhile, water quality in the pond was observed in the normal range with the temperatures of 28 - 30 oC, pH (6.0 - 7.0), DO (4.5 - 5.7 ppm), ammonia (0.06 - 0.03 ppm), nitrite (0 ppm) and nitrate (0 - 5 ppm).Â Keywords: Type of bacteria, catfish (Clarias gariepinus), Closed water system.

The Effect of Giving Combination Concentration of Leaves of Moringa oleifera with Walne Fertilizer in Culture Media on the Growth and Content of Carotenoids in Dunaliella salina Wahyuni, Nurita; Rahardja, Boedi Setya; Azhar, Muhammad Hanif
Journal of Aquaculture Science Vol 4, No 1 (2019): Journal of Aquaculture Science
Publisher : Airlangga University

The purpose of this study was to determine the effect and optimal dose of addition of Moringa oleifera leaf extract on culture media to the growth and content of carotenoids of Dunaliella salina. The research method used was experimental with Completely Randomized Design (CRD) as the experimental design. The treatments used were different doses of Moringa leaf extract, namely A (100% walne media), B (75% Media walne + 25% Moringa leaf extract), C (50% Media walne + 50% Moringa leaf extract), D ( 25% Media walne + 75% Moringa leaf extract), E (100% Moringa leaf extract) with a dose of Moringa leaf extract that is 28 ml (100%) with repetitions 4 times. The results showed that the addition of Moringa oleifera leaf extract had a significantly different effect (P <0.05) on growth. Based on the density of Dunaliella salina showed the best treatment, namely at treatment B (75% walne + 25% Moringa extract). The best specific growth rate is found in treatment B (75% walne + 25% Moringa extract). The carotenoid content after treatment was not significantly different (P> 0.05) so that the carotenoid content in the control (treatment A) had the highest level of 1.39 Âµg / ml and the lowest was in treatment C of 0.19 Âµg / ml.

Virus Detection of Pacific White Shrimp (Litopenaeus vannamei) at Fish Quarantine Center, Quality Control, and Security of Fishery Product in Surabaya I Azizah, Aulia; Budi, Darmawan Setia; Fasya, Arif Habib; Kenconojati, Hapsari; Azhar, Muhammad Hanif
Journal of Aquaculture Science Vol 4, No 2 (2019): Journal of Aquaculture Science
Publisher : Airlangga University

Pacific white shrimp (Litopenaeus vannamei) is one of the leading fisheries commodities that have important economic value and are in great demand by consumers in the market. Pacific white shrimp has several advantages, namely rapid growth, able to adapt to a high range of salinity, and can be cultured with a super intensive system, but in recent years the total shrimp production in Indonesia has decreased. In 2012 the total shrimp production decreased from 1.900 tons to 1.025 tons, the virus is thought to be a pathogen that triggers disease in shrimp and causes high mortality. The type of virus that causes disease in pacific white shrimp cultivation is Taura Syndrome Virus (TSV), Infectious Myonecrosis Virus (IMNV), and White Spot Syndrome Virus (WSSV). The purpose of this study is to detect viruses that infected and determine the prevalence of viruses that attack pacific white shrimp. The research was conducted in December 2018 to January 2019 at Fish Quarantine Center, Quality Control and Security of Fishery Product Surabaya I. The material used in the study was 37 post larval samples. Samples extracted using Silica Extraction Kit, then detected molecularly using PCR (Polymerase Chain Reaction). This research was observational, then the data obtained were analyzed descriptively. The results showed that there were 3 positive samples of IMNV and 2 positive samples of WSSV with prevalence values of 8.10% and 5.40%. High and low prevalence values are influenced by the level of virus spread and inappropriate environmental conditions.Â Keywords : Pacific white shrimp, PCR (Polymerase Chain Reaction), prevalence, virus.Â

The Insidensi dan Derajat Infeksi Anisakiasis pada Ikan Hasil Tangkapan di Pelabuhan Perikanan Pantai Muncar, Banyuwangi, Jawa Timur (INCIDENCE AND DEGREE OF ANISAKIASIS INFECTION IN FISH CATCHES AT THE FISHERY PORT OF MUNCAR BEACH, BANYUWANGI, EAST JAVA) Mohammad Faizal Ulkhaq; Darmawan Setia Budi; Muhammad Hanif Azhar; Hapsari Kenconojati
Jurnal Veteriner Vol 20 No 1 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

The purpose of this study was to determine the incidence and degree of infection anisakiasis in fish catches at Fishery Seaport Muncar, Banyuwangi as an effort to prevent food borne diseases in humans. Fish samples from Muncar Beach Fishery Seaport, Banyuwangi consisted of tuna fish (Euthynnus sp.), bloated fish (Rastrelliger sp.), giant trevally fish (Caranx sp.) and snapper (Lutjanus sp.) with total amount 192 tail. Sample fish was dissected and examined the parasite by native method. Parasitic worms was fixed with 5% glycerine alcohol, stained with SemichenAcetic Carmine method, identified by key identification and calculated incidence and degree of infection. The highest incidence and degree of infection showed in Lutjanus sp. and Rastrelliger sp. 66.67% and 5.39, respectively, while the lowest showed in Caranx sp. from 6.67% and 0.09 respectively. Continue research to determine incidence and degree of infection anisakiasis in each season was required to mapping and to know the spread of worms Anisakis sp.

Organic carbon source and C/N ratio affect inorganic nitrogen profile in the biofloc-based culture media of Pacific white shrimp (Litopenaeus vannamei) Muhammad Hanif Azhar; Eddy Supriyono; Kukuh Nirmala; Julie Ekasari
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 21, No 1 (2016): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Organic carbon source and C/N ratio play an important role in aquaculture system with biofloc technology application. Nitrogen control by adding carbohydrates to the water to stimulate heterotrophic bacterial growth by converting nitrogen into bacterial biomass. The study investigated the effect molasses, tapioca, tapioca by product and rice bran as carbon sources in a biofloc media at three different C/N ratios i.e. 10, 15, and 20 on total ammonia reduction in biofloc media. Five liters of biofloc media in a conical tank was prepared for each replicate, which consisted of 500 mL of biofloc suspension collected from a shrimp culture unit with biofloc technology application and 4.5 L seawater. Pacific white shrimp culture was performed in 40L glass aquaria at a shrimp density of 30/aquarium. There was a significant interaction between carbon source and the C/N ratio applied (P<0.05). The use of molasses resulted in the highest reduction rate irrespective to the C/N ratio. Keywords: molasses, tapioca, tapioca by product, rice bran, biofloc, total ammonia nitrogen

Dominansi dan Diversitas Lamun dan Makrozoobenthos pada Musim Pancaroba di Pantai Bama, Taman Nasional Baluran, Situbondo [Dominance and Diversity of Seagrass and Macrozoobenthos on Transition Season in Bama Beach, Baluran National Park, Situbondo] Sapto Andriyono; Daruti Dinda Nindarwi; Hapsari Kenconojatia; Daruti Dinda Nindarwi; Darmawan Setia Budia; Muhammad Hanif Azhar; Mohammad Faizal Ulkhaq
Jurnal Ilmiah Perikanan dan Kelautan Vol. 8 No. 1 (2016): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

AbstrakLamun merupakan tanaman berbiji terbuka yang mampu tumbuh dan beradaptasi dengan lingkungan bersalinitas tinggi serta dapat berasosiasi dengan benthos. Keberadaan lamun di perairan merupakan salah satu indikator tingkat kesuburan dan produktivitas perairan. Terdapat perbedaan dominansi antara musim hujan dan musim kemarau, sehingga enelitian ini bertujuan untuk mengidentifikasi dan menghitung dominansi dan keanekaragaman lamun dan makrozoobenthos pada musim pancaroba di Pantai Bama, TN Baluran, Situbondo. Metode penelitian yang digunakan yaitu line transect quadran dengan 5 line transek quadran yang masing-masingnya dipasang 5 plot transek. Ditemukan jenis lamun yang memiliki nilai kelimpahan tertinggi di Pantai Bama, TN Baluran pada musim pancaroba yaitu C. serrulata dengan nilai 48,90% , dan makrozoobenthos dari genus Holothuria dengan nilai 52,06%. Nilai Indeks dominansi (D) mengkategorikan Pantai Bama, TN Baluran dalam perairan dengan dominansi sedang. Sedangkan berdasarkan Indeks keanekaragaman (H’) mengkategorikan Pantai Bama, TN Baluran dalam perairan dengan keanekaragaman rendah. Musim peralihan berpengaruh terhadap jenis, kelimpahan relatif, indeks dominansi dan indeks keanekaragaman lamun dan makrozoobenthos di Pantai Bama, TN Baluran dibandingkan musim kemarau dan hujan. Perlu dilakukan survei secara berkala untuk mengetahui keanekaragaman organisme yang berkaitan dengan produktivitas perairan. AbstractSeagrass is the one of plants that can grow and adaptataion with high salinity environment and associated with benthos. Seagrass presence that indicate the productivity and prosperity in waters environtment. Found a different dominance between rainy season and dry season, so The aims of this study to identify and count dominance and diversity of seagrass and macrozoobenthos on the transition season in Bama Beach, TN Baluran, Situbondo. The method was used is line transect quadran with 5 quadran each of which installed 5 plot transect. Seagrass species was founded that have the highest abundance values in Bama Beach, TN Baluran the transitional season is C. serrulata with a value of 48.90%, and the macrozoobenthos that found from genus Holothuria with a value of 52.06%. Dominance index value (D) categorize Bama Beach, TN Baluran with moderate dominance. While based on the diversity index (H ') categorizes Bama Beach, TN Baluran with low diversity. Transitional seasons affect the type, relative abundance, dominance index and seagrass diversity index and macrozoobenthos in Bama Beach, TN Baluran than the dry and rainy seasons. Needed to investigate the diversity of organism that related to primary produktivity.

Abundance and Diversity of Bacteria on Catfish Nursery Rearing (Clarias gariepinus) With Closed Water System (Close Water System) Muhammad Hanif Azhar; Mohammad Faizal Ulkhaq; . Prayogo
Journal of Aquaculture Science Vol 2 No 1 (2017): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v2i1.19

This research aimed to identify types of bacteria existed in nursery rearing pond of catfish (Clarias gariepinus) using closed water system. The observed data include bacterial abundance and diversity in the water and in the fish digestion system, fish growth and water quality. This research was performed in the media of 3 concrete pools with the size of 3 x 1 x 0.8 m3 containing fish stock spreading of 3000 tail / pond. The obtained results showed that the highest abundance of bacteria was found in the surface and the bottom of pond C with the abundance number of 3,0 x 104and 1,2 x 103for aerobic and anaerobic bacteria, respectively. Moreover, it was figured that the dominant bacterial species found in the water and the gastrointestinal tract were from the genus of Bacillus, Clostridium and Lactobacillus. Meanwhile, water quality in the pond was observed in the normal range with the temperatures of 28 - 30 oC, pH (6.0 - 7.0), DO (4.5 - 5.7 ppm), ammonia (0.06 - 0.03 ppm), nitrite (0 ppm) and nitrate (0 - 5 ppm). Keywords: Type of bacteria, catfish (Clarias gariepinus), Closed water system.

The Effect of Giving Combination Concentration of Leaves of Moringa oleifera with Walne Fertilizer in Culture Media on the Growth and Content of Carotenoids in Dunaliella salina Nurita Wahyuni; Boedi Setya Rahardja; Muhammad Hanif Azhar
Journal of Aquaculture Science Vol 4 No 1 (2019): Journal Of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v4i1.67

The purpose of this study was to determine the effect and optimal dose of addition of Moringa oleifera leaf extract on culture media to the growth and content of carotenoids of Dunaliella salina. The research method used was experimental with Completely Randomized Design (CRD) as the experimental design. The treatments used were different doses of Moringa leaf extract, namely A (100% walne media), B (75% Media walne + 25% Moringa leaf extract), C (50% Media walne + 50% Moringa leaf extract), D ( 25% Media walne + 75% Moringa leaf extract), E (100% Moringa leaf extract) with a dose of Moringa leaf extract that is 28 ml (100%) with repetitions 4 times. The results showed that the addition of Moringa oleifera leaf extract had a significantly different effect (P <0.05) on growth. Based on the density of Dunaliella salina showed the best treatment, namely at treatment B (75% walne + 25% Moringa extract). The best specific growth rate is found in treatment B (75% walne + 25% Moringa extract). The carotenoid content after treatment was not significantly different (P> 0.05) so that the carotenoid content in the control (treatment A) had the highest level of 1.39 µg / ml and the lowest was in treatment C of 0.19 µg / ml.

Virus Detection of Pacific White Shrimp (Litopenaeus vannamei) at Fish Quarantine Center, Quality Control, and Security of Fishery Product in Surabaya I Aulia Azizah; Darmawan Setia Budi; Arif Habib Fasya; Hapsari Kenconojati; Muhammad Hanif Azhar
Journal of Aquaculture Science Vol 4 No 2 (2019): Journal Of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v4i2.71

Pacific white shrimp (Litopenaeus vannamei) is one of the leading fisheries commodities that have important economic value and are in great demand by consumers in the market. Pacific white shrimp has several advantages, namely rapid growth, able to adapt to a high range of salinity, and can be cultured with a super intensive system, but in recent years the total shrimp production in Indonesia has decreased. In 2012 the total shrimp production decreased from 1.900 tons to 1.025 tons, the virus is thought to be a pathogen that triggers disease in shrimp and causes high mortality. The type of virus that causes disease in pacific white shrimp cultivation is Taura Syndrome Virus (TSV), Infectious Myonecrosis Virus (IMNV), and White Spot Syndrome Virus (WSSV). The purpose of this study is to detect viruses that infected and determine the prevalence of viruses that attack pacific white shrimp. The research was conducted in December 2018 to January 2019 at Fish Quarantine Center, Quality Control and Security of Fishery Product Surabaya I. The material used in the study was 37 post larval samples. Samples extracted using Silica Extraction Kit, then detected molecularly using PCR (Polymerase Chain Reaction). This research was observational, then the data obtained were analyzed descriptively. The results showed that there were 3 positive samples of IMNV and 2 positive samples of WSSV with prevalence values of 8.10% and 5.40%. High and low prevalence values are influenced by the level of virus spread and inappropriate environmental conditions. Keywords : Pacific white shrimp, PCR (Polymerase Chain Reaction), prevalence, virus.

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