Ahmad A. Jusuf
The Department of Histology, Faculty of Medicine, Universitas Indonesia, Jakarta/ ICMR, School of Medicine, Kobe University/Division of Developmental Genetics, School of Medicine, Chiba University

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Iliac crest and femoral bone marrow as the source of plastic-adherent cells Phedy, Phedy; Dilogo, Ismail H.; Jusuf, Ahmad A.; Kholinne, Erica; Efendi, Zuhri
Medical Journal of Indonesia Vol 20, No 2 (2011): May
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (148.332 KB) | DOI: 10.13181/mji.v20i2.436

Abstract

Background: Many studies have used iliac crest as the source of mesenchymal stem cells. In cases of long bone shaft fracture, obtaining marrow from the fracture site offers more advantages. Nevertheless, due to the high number of fat cells in long bones, the yellow marrow of long bones is believed to contain lower number of mesenchymal stem cells than red marrow. Therefore the aim of this study is to compare the potency between red and yellow marrow as the donor site for the isolation of mesenchymal stem cell.Methods: Bone marrow of eight giant Flemish rabbits was aspirated from the iliac crest and femoral shaft. Mononuclear cells were isolated from both aspirates and expanded in low glucose DMEM. After eight weeks, the cells were harvested and counted using improved Neubauer hemocytometer. Comparison of the cell number between the two donor sites was then performed by t-test.Results: After 8 weeks, an average number of 2.93 ± 0.91 x 104 and 3.7 ± 2.50 x 104 cells were obtained from the iliac and femoral group respectively. No statistically significant difference was found between those two groups (p = 0.45).Conclusion: Plastic-adherent cells can be isolated and expanded from both iliac crest and femoral shaft. (Med J Indones 2011; 20:100-4)Keywords: plastic-adherent cells, red marrow, yellow marrow
Transgenic and gene disruption techniques from a concept to a tool in studying the basic pathogenesesis of various human diseases Jusuf, Ahmad A.
Medical Journal of Indonesia Vol 7, No 2 (1998): April-June
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1254.581 KB) | DOI: 10.13181/mji.v7i2.777

Abstract

[no abstract available]
Altered expressions of endothelial junction protein of placental capillaries in premature infants with intraventricular hemorrhage Ekawati, Maria; Mujihartini, Ninik; Jusuf, Ahmad A.; Dharmasetiawani, Nani; Jusman, Sri W.A.; Sadikin, Mohamad
Medical Journal of Indonesia Vol 25, No 3 (2016): September
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (691.713 KB) | DOI: 10.13181/mji.v25i3.1287

Abstract

Background: Placental hypoxia may lead to oxidative stress, which inflicts damage to capillary protein junction. The aim of this study was to evaluate altered expression of endothelial junction protein of capillaries in hypoxia condition and to observe its correlation with the incidence of  intraventricular hemorrhage in premature infants.Methods: A cross-sectional study was conducted by using placental tissues of premature infants as amodel of capillary integrity (29 hypoxic and 29 non-hypoxic). Hypoxia inducible factor (HIF)-1α was measured to define placental tissue response to hypoxia; malondialdehyde (MDA) and glutathione (GSH) served as markers of oxidative stress. The expressions of junctional proteins, N-cadherin and occludin were analyzed by immunohistochemistry. Intraventricular hemorrhage (IVH) was detected by cranial ultrasound at the third day. Unpaired t test, Mann-Whitney, and Chi-square tests were used to analyze the data.Results: The HIF-1α and MDA levels were slightly, but not significantly, higher in hypoxia group {13.64±8.70 pg/mg protein and 10.31 pmol/mg tissue (ranged 1.92–93.61), respectively}  compared to non- hypoxia group {10.65±5.35 pg/mg protein and 9.77 pmol/mg tissue (ranged 2.42–93.31)}. GSH levels were not different in both groups (38.14 (ranged 9.44–118.91) and  38.47(ranged 16.49–126.76) ng/mg protein, respectively. mRNA expression of N-cadherin (0.13) and occludin (0.096) were significantly lower in hypoxia comparedto non-hypoxia group (p=0,001), while protein expression of  N-cadherin (3.4; 75.9; 6.9; 13.8%) and occludin  (20.7; 3.4; 69.0; 3.4; 6.9%)  in hypoxia group was not associated with IVH (p=0.783 and p=0.743).Conclusion: Hypoxia altered expression of endothelial junction protein in placental capillaries, but no association with intraventricular hemorrhage was observed.