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All Journal Agrotrop : Journal on Agriculture Science International Journal of Biosciences and Biotechnology Buletin Udayana Mengabdi E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology)

I Wayan Dana Atmaja

Program Studi Agroekoteknologi Fakultas Pertanian Universitas Udayana

Author-ID : 2952510

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science

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ISOLATION AND IDENTIFICATION OF AZOTOBACTER OF SOME TYPE OF LAND USE IN JEGU VILLAGES Ni Nengah Soniari; I Wayan Dana Atmaja
International Journal of Biosciences and Biotechnology Vol 6 No 2 (2019)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Conventional farming systems have a negative impact on the life of Azotobacter. Through the results of this study, we want to provide information on the existence of Azotobacter in several rhizosphere of cultivated plants, and looking for isolates that have advantages as biofertilizers and decomposers. This research is an exploration of bacteria Azotobacter spp. from several plant rhizosphere namely: organic and inorganic rice paddy, cassava, coconut, and chocolate. Three samples was taken from each type of land use, so that the number of isolate sources were 15 samples. All analyzes were conducted at Soil Biology Laboratory, Faculty of Agriculture Udayana University. This study aims to find isolates of Azotobacter spp. which is superior to be utilized as biofertilizer and decomposer. Parameters used to support isolation and identification results are total population of bacteria Azotobacter spp., soil respiration, gram staining, halo zone and optical density. The results showed that organic rice rhizosphere was the best isolate source compared with inorganic rice rhizosphere, coconut, cassava and chocolate. Isolate from this organic rice rhizosphere has the highest total population (40.10 cfu x107g-1 soil), on positive catalase test yield bubbles and optical density (average 1.217ABS at 550 nm wavelength). While the superior isolates of Azotobacter spp. As biofertilizer and decomposer candidates are TSO2 isolates (samples from organic rice plant rhizosphere) with soil respiration rate (8.057mgC-CO2 kg-1 soil/day), high optical density (1.147 ABS on spectrophotometer with 550 nm wavelength) and highest halo zone diameter (10 mm).

PELATIHAN PEMBUATAN PUPUK ORGANIK YANG BERKUALITAS DARI LIMBAH PETERNAKAN SAPI DAN BABI DI DESA MARGA DAUHPURI, KECAMATAN MARGA, KABUPATEN TABANAN MEGA I M; I W.DANA ATMAJA; ID .OKA WIDYARSHANA; I A. SUTY ADNYANI; I N. DIBIA; DWI PUTRA DARMAWAN
Buletin Udayana Mengabdi Vol 8 No 1 (2009): Volume 8 No.1 – April 2009
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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ABSTRACT The training programme of making the qualities organic fertilizers derived from waste livestock cattle and pig were conducted at Marga Dauhpuri village, Marga District, Regency of Tabanan, from June until October 2008. The purpose of this programme was improving society skill on making organic fertilizers.The methods of programme were : !) giving counseling to livestock farmers who merged into group of Livestock Sari Buana about : environmental contamination problem by waste of livestock of cattle and pig, and way of the settlement of disposal 2) Training of making organic fertilizers ( compost) having quality from waste of ranch of cattle and pig. The results of devotion activity to this society were 1) can improve the society awareness specially group of farmer of livestock Sari Buana about : a) livestock waste deriving from cattle and pig become the economic valuable substance in order not to spoil environment b) Dirt of pig and cattle become the organic manure (compost) which is good for crop 2) Can improve skilled in making organic fertilizers ( compost) having quality from dirt of cattle and pig 3) The yielding of certifiable compost with the characteristic : dark brown color, granulous refine, not smell and content of element nutrient namely : C-Organic ( 3.04 %); N-Total ( 0.41 %), available-P ( 20.56 ppm), available-K ( 842.31 ppm), C / N ( 7.41) for the compost of cattle dirt, and C-Organic ( 3.70 %); N-Total ( 0.16 %), available-P ( 35.91ppm), available-K ( 2517.10 ppm), C / N (23.13) for the compost of pig dirt

PEMBUATAN DAN PENGUJIAN KUALITAS KOMPOS DI SUBAK TIBUBIYU KECAMATAN KERAMBITAN KABUPATEN TABANAN I.W. Narka; I.N. `Dibia; T. Kusmawati; I.W.D. Atmaja
Buletin Udayana Mengabdi Vol 17 No 1 (2018): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Devotion in the form of training of composting with existing materials around the farmers using 3 starter thatis EM4, MOL and Nitrobacter TJ has been done in Subak Tibubiyu, Kerambitan Tabanan. After subsequentcomposting is testing the quality of the three types of compost produced. The topic of composting andcompost quality testing is selected based on the farmers' demand because it is pointed out by the organicfertilizer farmers that the packaging is obtained in the form of aid, the quality is not good. Is a problem alsoin terms of transport to the planting area? Therefore, it is necessary to make compost near the farmer'scultivation area. In improving the quality of compost has been tried to make compost with 3 types of starterand then done the quality of compost testing. Farmers welcomed this activity and were interested to find outwhich type of starter was best composted. After fermentation for 6 weeks the compost has matured, then thequality of the compost is tested. The results of compost testing in the laboratory show that the best compostquality is compost with EM4 starter. With the success of farmers to make their own good quality compostfertilizer, the acceleration of organic fertilizer will be done. The use of compost fertilizer can reduce the useof chemical fertilizers and maintain the sustainability of the soil.

PENGOLAHAN LIMBAH PERTANIAN DAN KERAJINAN MENJADI PUPUK ORGANIK BERKUALITAS DI DESA TARO, KECAMATAN TEGALLALANG KABUPATEN GIANYAR A.A.N.G. Suwastika; N. W. S. Sutari; A.A.A.A. S. Sunari; N.N. Soniari; I W. D. Atmaja Atmaja
Buletin Udayana Mengabdi Vol 12 No 1 (2013): Volume 12 No.1 – April 2013
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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This community service was held on Thursday, July 26, 2012 at Gapoktan Sarwa Ada”, the village of Taro,Tegallalang District, Gianyar regency. The method used in this activity through education and training with theaim at improving farmers’ knowledge in the processing agricultural waste and waste wood craft a quality organicfertilizer to use local microorganisms. Training wass going well and in the family atmosphere. Material extensionand how to manufacture quality organic fertilizer were easily understood by farmers. All farmers can make theirown organic fertilizer from organic waste. Preparation of local microorganisms as decomposers bioactivator was easyto handle. Knowledge of farmers on organic waste still needs to be improved to prevent environmental pollution.

Pengaruh Dosis Pupuk Kascing Terhadap Hasil Tanaman Sawi (Brassica juncea L.), Sifat Kimia Dan Biologi Pada Tanah Inceptisol Klungkung KOMANG MELATI NUSANTARI KUSUMA SINDA; NI LUH KARTINI; I WAYAN DANA ATMAJA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.4, No.3, Juli 2015
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT The Effectt of Vermicompost Dose on Mustard Plant (Brassica juncea L.) Result, Some Characteristics of Chemistry and Biological Inceptisol Soil Klungkung This study was carried out on Inceptisol soil in Timuhun village Banjarangkan District, Klungkung and Soil Laboratory of Agroecotechnology Departemen / Studi Program, Faculty of Agriculture, Udayana University on March until April 2014. The purpose of this study was to determine the effect of vermicompost dose on Mustard Plant product, some chemical, biological soil, characteristics and determine the best dose vermicompost. The study design was a Randomized Group Design. The treatments used are organic vermicompost consisting of 9 levels i.e. O0 : Control; O1 : 2,5 ton ha-1 ; O2 : 5,0 ton ha-1 ; O3 : 7,5 ton ha-1; O4 : 10,0 ton ha-1 ; O5 : 12,5 ton ha-1 ; O6 : 15,0 ton ha-1 ; O7 : 17,5 ton ha-1 ; O8 : 20,0 ton ha-1. Each treatment was repeated 3 times so that required 27 experimental plots. The observation result of mustard plants made by counting the number of plants leaves, measuring fresh plot and dry plot weight. Analysis of chemical characteristic is done by calculating the total-N soil, available P, organic C, and soil pH. Analysis of the biological characteristics of the soil is done by calculating the population of microorganism. The results showed that vermicompost heavily influence the mustard plant product, chemical and biological soil characteristics. Vermicompost dose could increase the number of leaves 1.33%; 8.79% of plot fresh weight 8.35% plot dry weight; 1,41% of total-N soil; 5.56% available-P soil; 3,11% C-organic soil; 0,07% soil pH and 12,89 total population of soil microorganisms. Keywords : mustard plants, vermicompost.

Eksplorasi Bakteri Selulolitik dari Sampah Organik Kota Denpasar NI MADE INDRA PUSPAWATI; I WAYAN DANA ATMAJA; NI WAYAN SRI SUTARI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.7, No.3, Juli 2018
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Exploration of Cellulolytic Bacteria from Organic Waste in DenpasarMunicipal waste is one of the problem that must be overcome in Denpasar City. Municipal waste volume of Denpasar City 2014 was 1,247,769 m3, Year 2015 was 1,294,696 m3, and 2016 was increased to 1,296,438 m3. Total of waste that produced by Denpasar City is 70% organic waste. This type of organic waste has a major component of lignocellulose consisting of three polymers namely, cellulose, hemicellulose, and lignin. Cellulose is a component of plant cell walls. Cellulose has a low digestibility so it takes a long time to be degraded. One of the microorganisms that can produce the enzyme is the cellulolytic bacteria. The purpose of this research was to find and identify isolate of cellulolytic bacteria isolated from organic waste of Denpasar City. This research was conducted from September to 2017 until March 2018. Organic municipal waste samples were taken from each District in Denpasar City each one sample. Bacteria from the organic waste was isolated dan identified by moleculer identification in order to know the cellulolytic bacteria spesies. Based on the result of the research, two isolates of bacteria with the highest cellulolytic indeks were B-6 and U-6 isolates that were 7.3 and 3,0. The formation of clear zones around the colony showed that the isolates had qualitatively measured cellulolytic activity. The identification of both isolates was found were the B-6 isolate had the same percentage as Lactobacillus acidophilus (95%) and U-6 isolate had similarity percentage with Enterobacter cloacae (94%).

Perbedaan Karakteristik Kotoran Cacing Tanah dari Lahan Sayuran Organik dan Konvensional di Kecamatan Baturiti BINTANG PURNIASARI; I WAYAN DANA ATMAJA; NI NENGAH SONIARI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.8, No.3, Juli 2019
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Characteristics Differences of Casting from Organic and Conventional Vegetables Field in Baturiti District Casting is solid waste released from the anus of earthworms, containing organic matter, and microorganisme that can provide nutrients in the soil so they can be directly absorbed by plants. This study discusses the characteristic differences of casting seen from the physical, chemical and biological properties of organic vegetable field and conventional vegetable field in Baturiti District. The research was conducted from October 2018 to April 2019. The study including in deciding the location, taking samples of earthworms with soil, acclimatization process for earthworms, and analysis of physical, chemical, and biological properties of casting. The results of physical analysis of casting in organic and conventional farms have the same texture, the sandy clay loam textures. The results of chemical analysis of casting showed pH, N-total, Amonium, Nitrate and P-available were higher in organic field than conventional field. The pH value in organic soil is 6,58 and conventional soil is 6,50; N-total in organic soil is 0,75% and conventional soil is 0,65%; amonium and nitrate in organic soils is 6,44% and 2,1% compared to conventional field 4,35% and 1,92%, and P-available on organic field 393,40 mg kg-1 and conventional field 130,22 mg kg-1. The results of biological analysis of bacteria and fungi shows higher value in organic field than conventional field. The population of bacteria and fungi in organic field was 20,35 x 108 spk g-1 of casting and 4,44 x 105 spk g-1 of casting whereas in conventional field 10,20 x 108 spk g-1 of casting and 2,99 x 105 spk g-1 of casting.

Perbedaan Sifat Biologi Tanah pada Beberapa Tipe Penggunaan Lahan di Tanah Andisol, Inceptisol, dan Vertisol GUSTI AGUNG AYU RATIH SARIDEVI; I WAYAN DANA ATMAJA; I MADE MEGA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.2, No.4, Oktober 2013
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Differences in the nature of Soil Biology in Several Types of Land Use Land Andisol, Inceptisol, and Vertisol The aim of this research is to find out the difference of biological properties of soil on several types of land use in Andisol, Inceptisol, and Vertisol soil. The research was conducted on November 2012 until January 2013, starting from sample collection preparation up to soil analysis. Soil sample collection was conducted at Batunya Village (Andisol soil), Kuwum Village (Inceptisol soil), and Pererenan Village (Vertisol soil). Soil sample was analyzed at Soil Laboratory, Faculty of Agriculture University of Udayana. The design used was Randomized Block Design (RAK) of nested pattern with 2 factors, that was soil type factor (T) consisted of Ta (Andisol Soil), Ti (Inceptisol Soil), and Tv (Vertisol Soil), and second factor of land use type (L) consisted of Ls (irrigated land use types with rice), Lc (mixed farming), and Lj (irrigated land use types with corn). The parameters observed in this research among others were total bacterial population, total fungi population, respiration, C-organic content, N-total content, the ratio of C/N and pH. Andisol soil with land use type of mixed farmland (TaLc) has the highest total of bacterial population, at 6,26 x 108 spk g-1 of soil, highest total fungi population and highest respiration. Inceptisol soil with land use type of mixed farmland (TiLc) has the highest total bacterial population, at 6,62 x 108 spk g-1 of soil, highest total fungi population and highest respiration. Vertisol soil with land use type of mixed farmland (TvLc) has the highest total bacterial population, at 6,07 x 108 spk g-1 of soil, highest total fungi population and highest respiration. Andisol, Inceptisol, and Vertisol with three types of land use that are rice field, mixed farmland, and corn field have different biological properties. The type of land use of mixed farmland has biological properties of soil higher than other types of land use. Key words: Type of soil, Type of land use, and Biological properties of soil

Analisis Kualitas Larutan Mol (Mikoorganisme Lokal) Berbasis Daun Gamal (Gliricidia Sepium) IDA AYU YADNYA SENI; I WAYAN DANA ATMAJA; NI WAYAN SRI SUTARI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.2, No.2, April 2013
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Quality Analysis of MOL (Local Microorganism) Solution Based on Gamal (Gliricidia sepium) Leaves The purpose of this research was to find out the effects of gamal (Gliricidia sepium) leaves concentration and term of fermentation to the quality of MOL (local microorganism) solution. The research was held on September 2012 until November 2012, at Soil Laboratory, Faculty of Agriculture, Udayana University. Randomizing block design with two factors, including gamal leaves concentration (K) and fermentation term (F). The observation parameters of this research were total population of bacteria, total population of fungi, N-total, C-organic, C/N ratio. The analysis of gamal leaves concentration and the fermentation term was highly significant (P<0,01) affect. The total high population of bacteria in the K1 treatment be found the treatment F3 , 4,30 x 108 cfu ml-1, the total high population of bacteria in the K2 treatment can be found in the F3 treatment is 6,37 x 108 cfu ml-1, the total high of population of bacteria in the K3 treatment can be found in the F3 treatment is 8,63 x 108 cfu ml-1, the total population of bacteria in the K4 can be found in the F3 treatment is 9,07 x 108 cfu ml-1. The total population of fungi in the K1 treatment can be found in the F3 is 8,23 x 105 cfu ml-1, the total population of fungi in the K2 treatment can be found in the F3 is 8,27 x 105 cfu ml-1, the total population of fungi in the K3 treatment can be found in the F3 is treatment is 8,67 x 105 cfu ml-1, the total population of fungi in the K4 treatment can be found F3 treatment is 10,20 x 105 cfu ml-1. Concentration of 600 g/l gamal leaves on three weeks of fermentation have the best quality of mol solution. Keywords: Local Microorganisms (MOL), gamal leaves, fermentation term

Pengaruh Pupuk Organik dan Anorganik terhadap Peningkatan Produksi Edamame (Glycine max L. Merr) pada Tanah Subgroup Vertik Epiaquepts di Pegok, Denpasar SHERLEYTA PARIPURNANI; I NYOMAN DIBIA; I WAYAN DANA ATMAJA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.7, No.1, Januari 2018
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Effect Organic Fertilizer and Chemical Fertilizer (NPK) Of Increased Soybean Production Edamame On Land Subgroup Vertik Epiaquepts On Experimental Garden Udayana University, Denpasar Edamame have important role in providing nutritious food for the world's population because of the high content of amino acids, every one gram of edamame amino acids contains 340 mg isoleucine, 480 mg leucine, 400 mg lysine, 310 phenylalanine, 200 mg tyrosine, 80 mg methionine, 110 mg cystine, 250 mg threonine, 90 mg tryptophan dan 330 mg valine. This research about influence of organic fertilizer and inorganic fertilizers to increase edamame production conducted in Research Field Faculty of Agriculture and Soil Science and Environmental Laboratories Faculty of Agriculture at Udayana University from August 2016 to November 2016. The research was conducted with a randomized block design (RBD). The measured parameters are soil parameters that are pH, organic C, N total, P and K provided and plants parameters that are plant height, number of branches, number of contains pods, percentage of contains pods, production contains pods per plot, the estimated production of contains pods per hectare and fresh stover weight. The results showed that adding of a combination of organic and inorganic fertilizers affects some growing crops and soil parameters were observed. The combination of organic and inorganic fertilizers are best used with a combination of 150 kg ha-1 Fertilizer NPK + 10 ton ha-1 petroganic (P3) the estimated productions are 22,3 ton fresh pods per hectare, while the lowest is in control (without fertilizer) (P0) that 10,70 ton per hectare.

Co-Authors A.A.A.A. S. Sunari A.A.N.G. Suwastika ANAK AGUNG GDE PREHATRESNA ADI ASMARA Anak Agung Istri Kesumadewi Anak Agung Ngurah Gede Suwastika ARDHIKA FAHRIZA HARVIANTO BINTANG PURNIASARI CHARLES ALEXANDER YAWAN DWI PUTRA DARMAWAN GUSTI AGUNG AYU RATIH SARIDEVI I A. SUTY ADNYANI I DEWA MADE ARTHAGAMA I GUSTI AYU PUTU PRATIWI I Made Mega I MADE WERAYOGA I N. DIBIA I Nyoman Dibia I Nyoman Puja I WAYAN BUNADA I WAYAN NARKA I.N. `Dibia ID .OKA WIDYARSHANA IDA AYU YADNYA SENI IDA BAGUS PUTU WAHYU PERMANA KHOSNUL KHOTIMAH KOMANG MELATI NUSANTARI KUSUMA SINDA MADE SRI SUMARNIASIH MEGA I M N. W. S. Sutari Ni Luh Kartini NI MADE INDRA PUSPAWATI Ni Nengah Soniari NI PUTU NENA LUSIANA NI PUTU SRI SUTARI SAWIDYA FITRIYANI SHERLEYTA PARIPURNANI STEFANIA DESYANI DERGONG T. Kusmawati

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