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SPECIES DETERMINATION OF GREEN ALGAE ISOLATED FROM JEPARA COASTAL REGION BASED ON MICROBIOLOGICAL, ECOPHYSIOLOGICAL AND MOLECULAR CHARACTERIZATION FOR IMPROVEMENT OF CAROTENOID PRODUCTION Hermin Pancasakti Kusumaningrum; Endang Kusdiyantini; Triwibowo Yuwono; Joedoro Soedarsono
JOURNAL OF COASTAL DEVELOPMENT Vol 10, No 1 (2006): Volume 10, Number 1, Year 2006
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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A local isolate of green algae called C1 from Jepara waters is usually used as a source for carotenoid supplement for animal fisheries in the local area. This indigenous algae has been successfully purificated. Although the local isolate was known as eucaryotic green algae Dunaliella, our previous molecular study by 18S rDNA analysis to determine the species of this organisms showed negative result. In order to improve carotenoid production especially detection of biosynthetic pathway from the organisms investigated in this study, the main purpose of this study was species determination of local isolate of green algae based on microbiologycal, ecophysiologycal and molecular characterization. The results of this research indicated that local isolate of green algae posseses Cyanobacteria characteristic, especially Synechocystis. Analyses with 16S rDNA sequence from genome of green algae isolates were also in accordance with these results, showing close similarities with Synechocystis 16S rDNA sequence. However, it should be noted that, instead of having Synechocystis dominant feature, it was also found that local isolate of green algae exhibit different characteristic in having chlorophyll a, chlorophyll b and lack of phycobillins. This character was typical for abberant Cyanophyta, Prochlorophyta.

Kontribusi Pakan Chlorella sp. dan Tetraselmis chuii terhadap Densitas Copepoda Hadi Endrawati; Muhammad Zaenuri; Endang Kusdiyantini; Hermin Pancasakti Kusumaningrum
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 13, No 1 (2008): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Keberhasilan kultivasi copepoda diantaranya dipengaruhi oleh jenis pakan fitoplankton. Penelitian ini bertujuan untuk mengetahui densitas copepoda yang diberi pakan Chlorella sp. dan Tetraselmis chuii. Penelitian ini dilaksanakan dari Mei hingga Desember 2005 di Laboratorium Biologi Oseanografi UNDIP. Copepoda diambil per bulan dari bulan Mei hingga Oktober 2005, dari Perairan Demak. Kultivasi copepoda menggunakan 6 beker bervolume 2 L dengan densitas awal 100 ind. / L dan 6 akuarium bervolume 20 L dengan densitas awal 1000 ind. / L. Chlorella sp. dengan densitas 15.200 sel / ml dan Tetraselmis chuii dengan densitas 11.000 sel / ml yang berasal dari Balai Besar Pengembangan Budidaya Air Payau (BBPBAP) Jepara, digunakan sebagai pakan copepoda. Determinasi densitas copepoda dilakukan per minggu dari setiap perioda kultivasi ( 3 minggu ). Perioda kultivasi diulang per bulan selama 6 bulan. Parameter suhu, salinitas dan pH diukur setiap hari. Copepoda yang dikultivasi pada media 2 I dan 201 dengan pakan Tetraselmis chuii menunjukkan densitas rata - rata berturut-turut 227 - 303 ind. / L, dan 2534 - 3808 ind. / L. Densitas copepoda pada media 2 L dan 20 L dengan pakan Chlorella sp. menunjukkan rata - rata 297 -377 ind. / L pada media 2 l, serta 3241 - 4824 ind. / L. Pakan Chlorella sp. dan Tetraselmis chuii memberikan kontribusi densitas copepoda dengan model yang sama. Mesklpun demikian pakan Chlorella sp. menghasilkan densitas copepoda yang lebih tinggi dibandingkan Tetraselmis chuii pada dua volume media kultivasi yang berbeda. Kata kunci: Copepoda, Chlorella sp., Tetraselmis chuii, densitas

Konsumsi Harian Copepoda terhadap Pakan Chlorella sp. pada Volume Media Kultivasi yang Berbeda Muhammad Zaenuri; Hadi Endrawati; Endang Kusdiyantini; Hermin Pancasakti Kusumaningrum
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 13, No 3 (2008): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Penelitian ini bertujuan untuk mengetahui konsumsi harian copepoda terhadap Chlorella sp. pada volume media kultivasi yang berbeda. Penelitian ini dilaksanakan dari Mei hingga Oktober 2005 di Laboratorium Biologi Oseanografi UNDIP. Copepoda diambil per bulan dari bulan Mei hingga Oktober 2005 di Perairan Demak. Chlorella sp. digunakan sebagai pakan copepoda, berasal dari Balai Besar Pengembangan Budidaya Air Payau (BBPBAP) Jepara, dengan densitas 15.200 sel/mL. Kultivasi copepoda menggunakan 3 beker 2 L dengan densitas awal 100 ind./L dan 3 akuarium 20 L dengan densitas awal 1000 ind./L. Pengamatan konsumsi harian copepoda terhadap Chlorella sp. dilakukan per 3 jam dari pukul 06.00 sampai 18.00. Perioda kultivasi diulang per bulan selama 6 bulan. Parameter suhu, salinitas, DO dan pH diamati setiap hari. Konsumsi rata-rata copepoda terhadap Chlorella sp. menunjukkan 19,05- 140,47 sel/mL/jam pada media 2 L dan 10,69—102,06 sel/mL/jam pada media 20 L. Model konsumsi copepoda terhadap Chlorella sp. menunjukkan fluktuasi dengan puncak yang berbeda pada Mei-Juli 2005, namun mempunyai pola yang sama pada perioda Agustus-Oktober 2005.Kata kunci : Copepoda, Konsumsi Harian, Chlorella sp.The aim of the research is to know the daily consumption of the copepod on Chlorella sp. cultivated in the different volume of media. The research was conducted from May to October 2005 at Laboratory of BiologicalOceanography, Diponegoro University. Copepods were collected monthly from Demak waters. The copepod cultivations were done using 3 bekers of 2 L and 3 aquariums of 20 L. A stocking density of 100 ind./L ofcopepod for 2 l media and 1000 ind. / l of copepod for 20 l media were used as tests biota per period of cultivation. Chlorella sp. from Balai Besar Pengembangan Budidaya Air Payau (BBPBAP) Jepara were givento copepod with density of 15.200 cell/mL. The daily copepod consumption from 06.00–18.00 was observed at 3 hours intervals. The observations were replied monthly for six month. The temperature, salinity, DO and pH were measured daily. The average daily consumption of copepod on Chlorella sp. cultivated in 2 l anad 20 L media were 19,05–140,47 cell/mL and 10,69–102.,6 cell/ml, respectively. The daily consumption of copepod reveal a fluctuated model with differents peak of the consumption during the May – July 2005, while the one of the August – October 2005 show the same model.Key words : Copepods, Daily Consumption, Chlorella sp

Pertumbuhan Juvenil Ikan Kerapu Macan (Epinephelus fuscoguttatus) yang Dipelihara dengan Padat Penebaran Berbeda Hadi Ajie Endrawati; Muhammad Ajie Zaenuri; Endang Pancasakti Kusdiyantini; Hermin Pancasakti Kusumaningrum
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 13, No 3 (2008): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Penelitian ini bertujuan untuk mengetahui pertumbuhan juvenil ikan kerapu macan (Epinephelus fuscoguttatus) yang dipelihara dengan padat penebaran yang berbeda. Penelitian ini dilaksanakan dari April hingga Mei 2006 di Laboratorium Biologi Oseanografi UNDIP. Percobaan dilakukan di akuarium berukuran 40 x 40 x 60 cm3, dengan media air laut 10 liter. Perlakuan yang diterapkan adalah ikan kerapu macan (E. fuscoguttatus dengan tingkat kepadatan 5, 10 dan 15 ekor per aquarium. Copepoda diberikan sebagai pakan alami dengankepadatan 50 ind /L air media. Pemeliharaan dilakukan selama 4 minggu. Hasil penelitian menunjukkan bahwa semakin tinggi padat penebaran, pertambahan bobot dan panjang yangdicapai ikan kerapu macan (Epinephelus fuscoguttatus ) semakin kecil yaitu berturut-turut 3.67± 0.17; 3.21 ± 0.06 dan 2.16 ± 0.22 gram dan 0.63 ± 0.1 ; 0.55 ± 0.017 dan 0.5 ± 0.05 cm masing-masing untuk penebaran 5, 10 dan 15 ekor per wadah. Hal ini menunjukkan adanya persaingan dan kanibalisme.Kata kunci: larva, kerapu macan, Epinephelus fuscoguttatus, padat penebaran, pertumbuhan

KOMUNITAS RHIZOBAKTERIA TANAMAN TEH DENGAN APLIKASI FORMULA BIOIMUNIZER (Chryseobacterium Sp DAN Bacillus Sp) BERDASARKAN GEN 16S rRNA Agnistisya Widaranti; Siti Nur Jannah; Hermin Pancasakti Kusumaningrum; Dwi Ningsih Susilowati
Jurnal Akademika Biologi Vol. 5 No. 4 Oktober 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Commodity of tea (Camellia sinensis) has an important role in the national economy, especially in the field of agro-industries. Based on data obtained from the Directorate General of Plantation, the tea plant productivity declined over the last few years. This is due to the pest attack which result in decreasing the productivity of the tea plant. Increased crop productivity of tea have been done, such as the use of herbicides and insecticides, but until now there is very little effort to increase the production of tea plants by the use of biological agents. Chryseobacterium sp and Bacillus sp are rhizobacteria in tea rhizosphere that could potentially be used as a biocontrol agent (bioimmunizer). The purpose of this study is to determine the community rhizobacteria in tea soil with the addition of bioimmunizer based on 16S rRNA gene using T-RFLP technique. The method used in this research is T-RFLP technique (Terminal Restriction Fragment Length Polymorphism) using enzymes Msp I and Rsa I. The calculated value are relative abundance, Shannon diversity index (H '), evenness index (E), and dominance index. The results of this study indicate that soil samples with the addition of bioimunizer consisting of Arthrobacter sp, Bacillus sp, Actinobacteria, and Chryseobacterium sp.Keyword: T-RFLP, Chryseobacterium sp, Bacillus sp

KONSTRUKSI PLASMID PRHA SEBAGAI PEMBAWA GEN ARAA PENYANDI ENZIM L-ARABINOSA ISOMERASE DARI Thermotoga thermarum M Masfuroh; Anto Budiharjo; Hermin Pancasakti Kusumaningrum
Jurnal Akademika Biologi Vol. 6 No. 3 Juli 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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D-tagatosa as natural low-calorie sweeterners is likely to be a sugar substitute for diabetics type II. D-tagatosa sweetness levels is by 92% the sweetness of sucrose, but only 38% of calories of sucrose. This study aimed to obtain a recombinant plasmid construction pRHA::araA. Results subcloning was then used in biotransformation processes produce D-tagatosa. Propagation vector soure pRHA was done with the cloning procedure in E. coli TOP’10. The process used the vector source cut with enzyme NcoI and XhoI and producing pRHA vectore for gene insertion araA. AraA gene amplification was done using Polymerase Chain Reaction (PCR) with primers of AITth-For and AITth-Rev. Ligation was done using T4 ligase enzyme and transformed in E. coli TOP’10 by heat-shock methods. E. coli was grown in LB medium Agar with ampicillin concentration of 100 mg/ml. Selection was made on a liquid LB and LB Agar with ampicillin concentration range of 100-200 mg/ml. Based on the result of electrophoresis visualization of the pRHA::araA recombinant plasmid isolation were negative. Keyword: L-arabinosa isomerase, araA, D-tagatosa, T. thermarum, E. coli

IDENTIFIKASI MOLEKULER TANAMAN PISANG RAJALAWE BERDASARKAN GEN INTERNAL TRANCRIBED SPACER (ITS) Firly Putri Fardilla; Hermin Pancasakti Kusumaningrum; w wijanarka
Jurnal Akademika Biologi Vol. 6 No. 1 Januari 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Banana is one type of horticultural commodities in a group of fruits that have a socio-economic value is high enough for the people of Indonesia. Bananas have different varieties, one banana type Rajalawe found in Central Java. Rajalawe molecular identification has not been done before. This study aims to determine the result of the identification of the molecular basis of Rajalawe based on genes Internal transcribed spacer (ITS), in search of identity and kinship Rajalawe. The study was conducted by isolating DNA using a method Rajalawe Doyle & Doyle, followed by ITS gene amplification and sequencing analysis. The results of gene amplification ITS produce PCR product of 643 bp. The base sequence of the sequencing results are used for the construction of phylogenetic trees. Sequence similarity analysis Rajalawe show 95% homology with Musa balbisiana and alkaline difference of 1%. Phylogenetic tree analysis showed Rajalawe have a close relationship with Musa balbisiana. However, bananas Rajalawe has several different characters with Musa balbisiana with different base sequences by 5% whereas the base sequence homology between the banana Musa balbisiana and Rajalawe with 95%.Keywords: Molecular Identification, Pisang Rajalawe, Universal Primer ITS, Musa balbisiana.

ANALISIS FILOGENETIK Curcuma zedoaria (TEMU PUTIH) BERDASARKAN GEN INTERNAL TRANSCRIBED SPACER (ITS) Yuliandini Pangestika; Anto Budiharjo; Hermin Pancasakti Kusumaningrum
Jurnal Akademika Biologi Vol. 4 No. 4 Oktober 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Curcuma zedoaria belongs to Zingiberaceae family which has a local name white turmeric. This local name is not only used by C. zedoaria, but also used by C. mangga and Kaempferia rotunda. This problem leads to incorrect selection of ingredients, so that the therapeutic effect can not to achieved. Therefore phylogenetic analysis is important to differentiate three types of these plants. Phylogenetic analysis illustrated the taxonomic classification of organisms based on evolutionary history. Sequence which used in this study was Internal Transcribed Spacer (ITS). ITS flanked by the coding region of 18S, 5.8S and 26S rDNA on each unit in a series of chromosomes. Aimed of this study was to perform phylogenetic analysis of C. zedoaria which from Indonesia based on ITS gene sequences. Methods included were DNA isolation using Doyle and Doyle method (1987), ITS gene amplification using ITS1 and ITS4 primer, analysis of ITS gene sequences using Basic Local Aligment Search Tool (BLAST), and construction of phylogenetic trees using MEGA6 by neighbor-joining tree method and bootstrap method with 1000 replications. DNA isolation resulted DNA concentration of 853 ng/µl and purity value of 2.17. Amplification of ITS region resulted in 700 bp product. Result of homology search showed C. zedoaria had homology with C. zedoaria voucher JLS 71432 clone 4 from Czezh Republic with 72 % homology and 3 % gap. Phylogenetic analysis showed that white turmeric collected in this study was C. zedoaria and different from C. mangga and K. rotunda. This study concluded that C. zedoaria different from C. zedoaria from the Czech Republic, however both were in a Curcuma monophyletic group.Keywords: Curcuma zedoaria, Internal Transcribed Spacer, phylogenetic, white turmeric

PELACAKAN FRAGMEN GEN PENYANDI ENZIM ß-KETOASIL-ACP SINTASE II (KAS II) DARI MESOKARP KELAPA SAWIT (ELAEIS GUINEENSIS JACQ. L.) Yohanes Chandrawijaya; Teuku Tajuddin; Hermin Pancasakti Kusumaningrum; Anto Budiharjo
Jurnal Akademika Biologi Vol. 2 No. 2 April 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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The standard of quality is one of the determining values of crude palm oil as an international trade commodity. Better standard of quality for crude palm oil is a constant demand of the market. Quality improvement can be made by increasing the contents of oleic acid in the mesocarp of E. guinensis. Among the uses of oleic acid are as follows: anti-carcinogenic agent, anti-oxidant, source of pro-vitamin A, and source of Vitamin E. Oleic acid is a form of non-saturated fatty acid encoded by KAS II genes. The expression profiling of KAS II is achieved through isolation of total RNA by Trizol reagent, RNA purification, using DNAse RNAse free, synthesis of cDNA using Reverse Transcriptation PCR approach, and amplification of KAS II genes with Nested PCR approach. The amplification process of KAS II genes is carried out using both internal and external primers. The first step of the external primer PCR is F-KAS-1 and R-KAS-1. Internal primer of PCR in the second step is F-KAS-2 and R-KAS-2. The results of this research are fragments of KAS II genes between 1500–2000 bp. These amplicons are suitable with primers designed at the approximation of 1796 bp. Selection of three amplicons at the annealing temperatures of 54oC, 55.9oC, and 58oC shows good DNA band visualizations. Annealing at 58oC shows the best result with high intensity DNA band and no smear. Further research is needed to determine the accuracy of the amplicons through sequencing step. Keywords: KAS II, Elaeis guineensis, annealing, Nested PCR, RT-PCR

ANALISIS KANDUNGAN β-KAROTEN FUSAN INTRASPESIES Chlorella vulgaris dan APLIKASINYA SEBAGAI PAKAN TAMBAHAN PADA POST LARVA STADIA 10 UDANG WINDU (Penaeus monodon). Dwi Kristyaningrum; Hermin Pancasakti Kusumaningrum; Endang Kusdiyantini
Jurnal Akademika Biologi Vol. 2 No. 3 Juli 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Carotenoids are one of pigment which needed for development of post-larvae tiger shrimp (Penaeus monodon), but the shrimp unable to synthesis carotenoids by themselves in the body so it takes a feed containing carotenoids. Carotenoids can be obtained from synthetic feed or natural feed. One of the natural feed is microalgae Chlorella vulgaris. β-carotene is the highest content of carotenoid in C. vulgaris, this content can be improved by protoplast fusion. The purpose of this study was to determine the amount of β-carotene in fusan intraspecies C. vulgaris and it’s application as supplement in the post-larvae stadia 10th tiger shrimp (P. monodon) Measurement of the amount of β-carotene using UV-Vis at a wavelength of 460nm. The feeding is done three times a day. The results showed that the amount of β-carotene on fusan C. vulgaris is greater than the wildtype of C. vulgaris. β-carotene in the fusan C. vulgaris is14.88µg/g while the wildtype of C. vulgaris is 7.14 µg/g. Additional applications C. vulgaris by 10 x 104 in 0.0225 g synthetys feed post-larvae stadia 10th shrimp (P. monodon) compared to the post-larvae shrimp fed only control showed that the feed given additional fusan of C. vulgaris increases the weight of shrimp at 39.9%, while the given wildtype of C. vulgaris increasing shrimp weight by 10.1%. Keywords: β-carotene, Chlorella vulgaris, protoplast fusion, post larvae shrimp

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