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BIOPROSPEKSI BAKTERI YANG BERASOSIASI DENGAN ALGA HIJAU HALIMEDA MACROLOBA, CAULERPA RACEMOSA, DAN ULVA SP SEBAGAI PENGHASIL SENYAWA ANTIBAKTERI Risky Panji Nugroho; Anto Budiharjo; Endang Kusdiyantini
Jurnal Akademika Biologi Vol. 2 No. 4 Oktober 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Bacteria can grow in various environments, including those associated with marine organisms such as algae, sponge, sea grass and soft corals. For these organisms, bacteria help building their defense by producing secondary metabolites such as bioactive compound.  This research aims to study the potency of the bacteria which interact with green algae Halimeda macroloba, Caulerpa racemosa, and Ulva sp which are able to produce antibacterial compound. Algae, which belong to Chlorophyta, are common to be found in marine water. This research used bacterial isolation, morphologic bacterial isolate characterization, antibacterial test, molecular identification of the antibacterial compound producer isolate, and biochemical activity test. From the isolation, the researcher was able to collect five bacterial isolate; one from H. macroloba, three from C. racemosa, and one isolate from Ulva sp. Of the five isolates, one isolate from C. racemosa can prevent the E.coli, and one isolate from H. macroloba can prevent E.coli and P.aeruginosa. Isolate derived from Halimeda macroloba have the biggest prevention zone ability, which is 18.1 mm, towards P.aeruginosa. Keywords: Antibacterial, Bacterial Association, Halimeda macroloba, Caulerpa racemosa,      Ulva sp
ISOLASI DAN KARAKTERISASI BAKTERI ASAM LAKTAT PADA PANGAN FERMENTASI MANDAI Mangasa Tua Pandapotan Siregar; Endang Kusdiyantini; MG Isworo Rukmi
Jurnal Akademika Biologi Vol. 3 No. 2 April 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

East Kalimantan has a lot of processed food products traditionally, one of them is mandai which is fermented food made from fruit leather Cempedak (Arthocarphus champeden). Lactic acid bacteria involved in the fermentation mandai greatly affect the quality of the final product. This study aimed to perform the isolation and characterization of lactic acid bacteria in the mandai fermentation process. MRS medium was used in the isolation of bacteria for 14 days. Isolation of bacteria during the fermentation process mandai obtaining 17 isolates, nine isolates Gram negative isolates, and eight isolates Gram positive. Eight isolates showed positive results against some of the morphological and biochemical characterization. Including biochemical characterization, acid formation and gas production from glucose, motility test, catalase test, growth at 160C and 480C, hydrolysis of starch, fat hydrolysis, and hydrolysis of casein and acid formation test. Eight isolates bacteria made mandai into processed food products that had a sour taste. Keywords: lactic acid bacteria, BAL ,bacterial isolates, cempedak skin.
ISOLASI, KARAKTERISASI BAKTERI ASAM LAKTAT, DAN ANALISIS PROKSIMAT DARI PANGAN FERMENTASI RUSIP IKAN TERI (Stolephorus sp.) Deverina Mergypta; Anto Budiharjo; Endang Kusdiyantini
Jurnal Akademika Biologi Vol. 3 No. 2 April 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Rusip is a traditional food from Bangka-Belitung made from fresh anchovy with salt and palm sugar that is brooded for seven days. Rusip fermentation process is caused by microbial activity mainly from lactic acid bacteria. The purpose of this study was to isolate lactic acid bacteria from rusip, to characterize isolates obtained based on common characteristic of lactic acid bacteria, and to carry out proximate analysis of rusip anchovy. Isolation from rusip fermented food obtained eight bacterial isolates, five isolates were circular and three isolates were rod-shaped. Characterization and identification of lactic acid bacterial were performed using tests that led to the common characteristic of lactic acid bacteria, i.e the gram staining, motility test, acid formation test, catalase test, and proteolytic test. The result showed that eight bacterial isolates were gram-positive, non-motile, catalase-negative, positive to produce acid, and had proteolytic activity. Rusip anchovy had 0,64% of total lactic acid and the pH 5,40. Nutrient content of rusip fermentation had 10,3644% moisture content; 50,0702% ash content; 0,9524% crude fat content; 0,3764% crude fiber content; 34,8603% crude protein content; and 14,1171% carbohydrate content. Keywords: rusip, isolation, characterization, lactic acid bacteria, proximate analysis
Konsumsi Harian Copepoda terhadap Pakan Chlorella sp. pada Volume Media Kultivasi yang Berbeda Muhammad Zaenuri; Hadi Endrawati; Endang Kusdiyantini; Hermin Pancasakti Kusumaningrum
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 13, No 3 (2008): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (156.299 KB) | DOI: 10.14710/ik.ijms.13.3.121-126

Abstract

Penelitian ini bertujuan untuk mengetahui konsumsi harian copepoda terhadap Chlorella sp. pada volume media kultivasi yang berbeda. Penelitian ini dilaksanakan dari Mei hingga Oktober 2005 di Laboratorium Biologi Oseanografi UNDIP. Copepoda diambil per bulan dari bulan Mei hingga Oktober 2005 di Perairan Demak. Chlorella sp. digunakan sebagai pakan copepoda, berasal dari Balai Besar Pengembangan Budidaya Air Payau (BBPBAP) Jepara, dengan densitas 15.200 sel/mL. Kultivasi copepoda menggunakan 3 beker 2 L dengan densitas awal 100 ind./L dan 3 akuarium 20 L dengan densitas awal 1000 ind./L. Pengamatan konsumsi harian copepoda terhadap Chlorella sp. dilakukan per 3 jam dari pukul 06.00 sampai 18.00. Perioda kultivasi diulang per bulan selama 6 bulan. Parameter suhu, salinitas, DO dan pH diamati setiap hari. Konsumsi rata-rata copepoda terhadap Chlorella sp. menunjukkan 19,05- 140,47 sel/mL/jam pada media 2 L dan 10,69—102,06 sel/mL/jam pada media 20 L. Model konsumsi copepoda terhadap Chlorella sp. menunjukkan fluktuasi dengan puncak yang berbeda pada Mei-Juli 2005, namun mempunyai pola yang sama pada perioda Agustus-Oktober 2005.Kata kunci : Copepoda, Konsumsi Harian, Chlorella sp.The aim of the research is to know the daily consumption of the copepod on Chlorella sp. cultivated in the different volume of media. The research was conducted from May to October 2005 at Laboratory of BiologicalOceanography, Diponegoro University. Copepods were collected monthly from Demak waters. The copepod cultivations were done using 3 bekers of 2 L and 3 aquariums of 20 L. A stocking density of 100 ind./L ofcopepod for 2 l media and 1000 ind. / l of copepod for 20 l media were used as tests biota per period of cultivation. Chlorella sp. from Balai Besar Pengembangan Budidaya Air Payau (BBPBAP) Jepara were givento copepod with density of 15.200 cell/mL. The daily copepod consumption from 06.00–18.00 was observed at 3 hours intervals. The observations were replied monthly for six month. The temperature, salinity, DO and pH were measured daily. The average daily consumption of copepod on Chlorella sp. cultivated in 2 l anad 20 L media were 19,05–140,47 cell/mL and 10,69–102.,6 cell/ml, respectively. The daily consumption of copepod reveal a fluctuated model with differents peak of the consumption during the May – July 2005, while the one of the August – October 2005 show the same model.Key words : Copepods, Daily Consumption, Chlorella sp
Pertumbuhan Mikroalga Botryococcus braunii Sebagai Penghasil Lipid Pada Medium Campuran Antara Air Kelapa Dan Air Laut Bintoro Rudi Saputro; Endang Kusdiyantini; Hermin Pancasakti Kusumaningrum
Jurnal Akademika Biologi Vol. 4 No. 4 Oktober 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Energy needs in fuel sector tend to increase for supporting human life. Green microalgae (Chlorophyceae) can be used as an alternative solutions  for bioenergy.  Botryococcus braunii is lipid producer microalgae which can be processed further into biodiesel. Microalgae growth medium such as Walne can be subtituted with coconut water that reducing economical cost. This research aims to determine the growth of B. braunii using coconut water and its effect on lipid production. The experiment conducted into five treatments of coconut water consist of  P0 (0%), P1 (10%), P2 (7.5%), P3 (5%), and P4 (2.5%) for five days incubation and three repetitive step. Microalgae growth was calculated according to cell count using haemocytometer. The results showed that 2.5% coconut water subtitution exhibited the best growth rate and lipid production, ie the amount of 547 cells / ml and produced lipid level according to these treatment increased 179% comparing with control. Keywords: Growth, Botryococcus braunii, Lipid, Coconut water, Seawater.
Kontribusi Pakan Chlorella sp. dan Tetraselmis chuii terhadap Densitas Copepoda Hadi Endrawati; Muhammad Zaenuri; Endang Kusdiyantini; Hermin Pancasakti Kusumaningrum
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 13, No 1 (2008): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (21.729 KB) | DOI: 10.14710/ik.ijms.13.1.43-48

Abstract

Keberhasilan kultivasi copepoda diantaranya dipengaruhi oleh jenis pakan fitoplankton. Penelitian ini bertujuan untuk mengetahui densitas copepoda yang diberi pakan Chlorella sp. dan Tetraselmis chuii. Penelitian ini dilaksanakan dari Mei hingga Desember 2005 di Laboratorium Biologi Oseanografi UNDIP. Copepoda diambil per bulan dari bulan Mei hingga Oktober 2005, dari Perairan Demak. Kultivasi copepoda menggunakan 6 beker bervolume 2 L dengan densitas awal 100 ind. / L dan 6 akuarium bervolume 20 L dengan densitas awal 1000 ind. / L. Chlorella sp. dengan densitas 15.200 sel / ml dan Tetraselmis chuii dengan densitas 11.000 sel / ml yang berasal dari Balai Besar Pengembangan Budidaya Air Payau (BBPBAP) Jepara, digunakan sebagai pakan copepoda. Determinasi densitas copepoda dilakukan per minggu dari setiap perioda kultivasi ( 3 minggu ). Perioda kultivasi diulang per bulan selama 6 bulan. Parameter suhu, salinitas dan pH diukur setiap hari. Copepoda yang dikultivasi pada media 2 I dan 201 dengan pakan Tetraselmis chuii menunjukkan densitas rata - rata berturut-turut 227 - 303 ind. / L, dan 2534 - 3808 ind. / L. Densitas copepoda pada media 2 L dan 20 L dengan pakan Chlorella sp. menunjukkan rata - rata 297 -377 ind. / L pada media 2 l, serta 3241 - 4824 ind. / L. Pakan Chlorella sp. dan Tetraselmis chuii memberikan kontribusi densitas copepoda dengan model yang sama. Mesklpun demikian pakan Chlorella sp. menghasilkan densitas copepoda yang lebih tinggi dibandingkan Tetraselmis chuii pada dua volume media kultivasi yang berbeda. Kata kunci: Copepoda, Chlorella sp., Tetraselmis chuii, densitas
KARAKTERISTIK MORFOLOGI, BIOKIMIA, DAN MOLEKULER ISOLAT KHAMIR IK-2 HASIL ISOLASI DARI JUS BUAH SIRSAK (Annona muricata L.) Vivi Suryaningsih; Rejeki Siti Ferniah; Endang Kusdiyantini
Jurnal Akademika Biologi Vol. 7 No. 1 Januari 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Yeast was found in foods that contain lots of sugar as fruits. Yeast utilizes simple sugars in food to gain energy. Yeast has a role in the natural fermentation of the fruit that resulting of decay, discoloration, and cause the fruit not durable. The purpose of this research were to isolate the yeast from the soursop fruit and to identify the yeast in morphology, biochemistry, and molecular test based on Internal Transcribed Spacer (ITS). Isolation of  yeast  was  performed by growing on  medium PDA and chloramphenicol. Identification was done through biochemical test by looking at the capabilities in the fermentation of carbohydrate and the abilities to grow on a medium to high osmotic pressure. Molecular identification was done using Internal Transcribed Spacer (ITS) region. The result showed that isolate IK-2 yeast from the soursop fruit juice had a distinctive form round to oval, prominent elevation, the colour creamy white, form a buds, able to ferment glucose and sucrose, but not able to ferment lactose, as well as being able to grow on media with glucose level 50 %. Molecular analysis of the ITS region using ITS1 and ITS4 primers, and phylogenetic analysis using Neighbor Joining. The result of the Basic Local Alignment Tools (BLAST) showed that the isolate had 95% homology with Candida tropicalis. Key Words : Yeast, Soursop (Annona muricata L.), Internal Transcribed Spacer (ITS)
IDENTIFIKASI SENYAWA BIOAKTIF PADA ISOLAT BAKTERI BUAH BELIMBING WULUH (Averrhoa bilimbi L.) SEBAGAI AGENSIA HAYATI Xanthomonas oryzae pv. oryzae Aniza Rachmawati; Agung Suprihadi; Endang Kusdiyantini
Jurnal Akademika Biologi Vol. 6 No. 3 Juli 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Bilimbi (Averrhoa bilimbi L.) is one of original fruit plants from Indonesia. Bilimbi fruits contain flavonoid, saponin, triterpenoid and tanin that have ability as anti-microbial. Bacteria was isolated from bilimbi fruits expected to be able produce bioactive compounds which can kill pathogenic bacteria such as Xanthomonas oryzae pv. oryzae which caused Bacterial Leaf Blight (BLB). Bacterial Leaf Blight in Indonesia caused harvest losses of 18 – 36 %. Infection was caused by X. oryzae caused the leaf symptoms to turn pale yellow, white, withered, and finally die. The purpose of this research are get bacteria isolate from bilimbi fruits (Averrhoa bilimbi L.) and testing bioactive compounds on bacteria isolate from bilimbi fruits which can inhibit Xanthomonas oryzae pv. oryzae (Xoo) growth. This research use methods, that are isolation of bacteria from bilimbi fruits and isolation of Xanthomonas oryzae pv. oryzae, characterization biochemically, inhibition test, and Thin Layer Chromatography. Obtained two bacteria isolate from bilimbi fruits. They are IBW1 dan IBW2, inhibition zona of IBW1 is 0,15 mm and IBW2 0,35 mm. Both of them have potential in antibacteria of X. oryzae bacteria eventhough in weak catagory. Metabolite secondary compound which play a role in antibacteria of X. oryzae is flavonoid compound. Kata kunci: antibacteria, bilimbi, Thin Layer Chromatography, X. oryzae
PRODUKSI PIGMEN OLEH ISOLAT KAPANG HASIL ISOLASI DARI ANGKAK KOMERSIAL DI SEMARANG PADA SUMBER N DAN pH BERBEDA Soni Nugraha Anwar; Endang Kusdiyantini; Arina Tri Lunggani
Jurnal Akademika Biologi Vol. 2 No. 4 Oktober 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

The development of the food processing industry led to the use of dyes have also increased, especially the type of synthetic dyes that can be harmful to consumers because of its toxicity. Natural dyes to be one of the alternatives used in the field of food. One of the natural dyes is widely used as a food coloring that is red yeast rice. Red yeast rice is rice that is overgrown by the mold Monascus sp. that produces pigment. This study aims to obtain pure isolates of red yeast rice molds that are in Semarang and knowing the growth and production of red pigment in the fungi isolates the different source of N and pH. The treatment is done by growing PDB (potato dextrose broth) in the medium with treatment medium pH 3,5,7 and 9 as well as optimization of the nitrogen source Ammonium chloride 1 %, Ammonium Nitrate 1 %, and Peptone 1 %. Analysis of pigments using a spectrophotometer with a wavelength (λ) of 500 nm and analysis of dry cell weight mycelia (g/l). The results showed the highest pigment concentration at treatment pH 7 with 0.812 absorbance value and the highest value of the cell dry weight at pH 7 is 1.232 g/l. Results of optimization with different nitrogen sources showed the highest pigment levels in the addition of a nitrogen source Ammonium Chloride 1 % to the value of 0.821 absorbance and dry weight of most cells are in Ammonium Nitrate is 2.556 g/l. Keywords: Pigment, Angkak, Isolate Fungus, pH, Nitrogen.
ANALISIS KANDUNGAN β-KAROTEN FUSAN INTRASPESIES Chlorella vulgaris dan APLIKASINYA SEBAGAI PAKAN TAMBAHAN PADA POST LARVA STADIA 10 UDANG WINDU (Penaeus monodon). Dwi Kristyaningrum; Hermin Pancasakti Kusumaningrum; Endang Kusdiyantini
Jurnal Akademika Biologi Vol. 2 No. 3 Juli 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Carotenoids are one of pigment which needed for development of post-larvae tiger shrimp (Penaeus monodon), but the shrimp unable to synthesis carotenoids by themselves in the body so it takes a feed containing carotenoids. Carotenoids can be obtained from synthetic feed or natural feed. One of the natural feed is microalgae Chlorella vulgaris. β-carotene is the highest content of carotenoid in C. vulgaris, this content can be improved by protoplast fusion. The purpose of this study was to determine the amount of β-carotene in fusan intraspecies C. vulgaris and it’s application as supplement in the post-larvae stadia 10th tiger shrimp (P. monodon) Measurement of the amount of  β-carotene using UV-Vis at a wavelength of 460nm. The feeding is done three times a day. The results showed that the amount of β-carotene on fusan C. vulgaris is greater than the wildtype of C. vulgaris. β-carotene in the fusan C. vulgaris is14.88µg/g while the wildtype of C. vulgaris is 7.14 µg/g. Additional applications C. vulgaris by 10 x 104 in 0.0225 g synthetys feed post-larvae stadia 10th shrimp (P. monodon) compared to the post-larvae shrimp fed only control showed that the feed given additional fusan of C. vulgaris increases the weight of shrimp at 39.9%, while the given wildtype of C. vulgaris increasing shrimp weight by 10.1%. Keywords: β-carotene, Chlorella vulgaris, protoplast fusion, post larvae shrimp